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The map label for this gene is tig

Identifier: 50843049

GI number: 50843049

Start: 1702280

End: 1703872

Strand: Reverse

Name: tig

Synonym: PPA1575

Alternate gene names: 50843049

Gene position: 1703872-1702280 (Counterclockwise)

Preceding gene: 50843054

Following gene: 50843048

Centisome position: 66.55

GC content: 59.89

Gene sequence:

>1593_bases
GTGCCCAGCAGCCTTGAGAAGCTCAGCACTAACCGTGTGAAGCTCACGATCGAGATGCCCTTCGATGAACTGAAGCCCAG
CCTTGACAAGGCCTACAAGGACATCGCCAACCAGGTGAACGTTCCCGGCTTCCGCAAGGGAAAGGTGCCCGCTCCGGTTA
TCGACCAGCGTTTCGGTCGCGGGGTCGTCCTTCAAGAGGCCATCAACGACGCCCTCCCGGCTGCTTATGGCAAGGCCATC
GAGGAGAACACGGTCGTTCCCTTGGGTCAGCCCGAGATCGAGGTGACCAAGCTGGAGGATGGCGAGGTCGTCGAGTTCAC
CGCCGAGGTGGACGTTCGTCCTGACTTCGATCTTCCCGACGTCTCCGCCATCTCCGTTGAGGTGCCTGCCGTTGAGGTGC
CGGACGAGGACGTCGACGAGCGCGTCGAGACCCTGCGCCAGCGTTTTGCCACTAATACGGAGGTCGAGCGCGCTGCTGCC
AAGGACGACCTCGTCACTATTGACCTTGCTGGAACCCGTGACGGCGAGGTTCTCGAAGACGCCACCGCATCCGGTGTCAC
CTACAAGGTCGGTTCCGAGGGCATGCTAGAAGGCCTTGATGAGGCTGTTACCGGTCTCAAGGCGGGTGAGTCCGCAGAGT
TCCACTCCACCCTCGTTGGCGGCCCGCTGCGCGGCCAGGATGCCGACATTAAAGTCACCGTTACTAAAGTCTGCGAGCAG
GAGCTCCCGGCCGTTGACGACGACTTCGCCCAGTTGGTCAGTCAGTTCGACACTGTCGACGAGATGCGTGCCGACTTGCG
CACCGCTTTGGAGAATATGGCTCGCCTCGACCAGGCTGCAGACGCCCGCGACAAGGTGCTGGAGGAGGTTATCTCCAAGA
TCGACATTGAGCTGCCGACCAACCTCATCGACTCTGAGCTCGAAGCCCGCCGTCAGCAGGTCAACCAGCAGTTGGCCCAG
GCTGGTATGACCGTCGAGGAGTATCTCGAAGACTCCGAAGAAGAGGTGGATAACGCCGACGACTTCTGGGCTGAGATCGA
GAAGCGTTCCCTGGACGCCCTCAAAGCCCAAATTGTCCTCGACAAGATGGCCGATGACGACGAGATCGGTGTTGAGCAGA
ACGAGTTGACTGAGTTGCTGTTCCGCAAGGCTCAGCAGAACGGCACCTCCCCCGAGGAGGAGGCTCAGCACATGATGCAG
CACAACCACCTGCCTGATTGGATGCAGGAGATCCGTCGTGGCAAGGCTCTGGCCTCCATGGTGGGTGCTGCTACCGTCAC
CGACTCGAAGGGTAATGCTCTCGAGCTTGACCGCATCCAGCCTGACGGCACCATTGCCGACAAGCAGGCCGAAGAGGACG
CTCCTGCTGAAGATTCTGCTGAGAAGGCTGACGGCAAGGCCACAGAGGAGTCCAAGGCTGAGGAGAAGGCTCCCGCTAAG
AAGGCGACCACCAAGAAGATTTCCGCTAAGAAGTCTGCTGGTGCCAAGGCGACCACCAAGAAGGTCGTAGACGCCAAGTC
TGACGACAAGCCCGCTGCTAAGAAGCCTGCTCCTAAGAAGAAGACGGCTGCCAAGGACGATAAGTCCAAGTGA

Upstream 100 bases:

>100_bases
AACCTTCCGGTGGCTCAGGAACTACTGCGTCGCTAGAATTAGATGGAATGTGTACTCCCCTCAACGGGGATGACAGAATC
GGAACCACAGGAGAAACTTC

Downstream 100 bases:

>100_bases
TGATCAGGGGCCACGTGTCCCGTAGGATCGACATGATCCGTGATGACCGGCGTCGTGCGGGTTTCCGCACGACGCCGGTC
CCACGTCAACCCTGAGGAAG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MPSSLEKLSTNRVKLTIEMPFDELKPSLDKAYKDIANQVNVPGFRKGKVPAPVIDQRFGRGVVLQEAINDALPAAYGKAI
EENTVVPLGQPEIEVTKLEDGEVVEFTAEVDVRPDFDLPDVSAISVEVPAVEVPDEDVDERVETLRQRFATNTEVERAAA
KDDLVTIDLAGTRDGEVLEDATASGVTYKVGSEGMLEGLDEAVTGLKAGESAEFHSTLVGGPLRGQDADIKVTVTKVCEQ
ELPAVDDDFAQLVSQFDTVDEMRADLRTALENMARLDQAADARDKVLEEVISKIDIELPTNLIDSELEARRQQVNQQLAQ
AGMTVEEYLEDSEEEVDNADDFWAEIEKRSLDALKAQIVLDKMADDDEIGVEQNELTELLFRKAQQNGTSPEEEAQHMMQ
HNHLPDWMQEIRRGKALASMVGAATVTDSKGNALELDRIQPDGTIADKQAEEDAPAEDSAEKADGKATEESKAEEKAPAK
KATTKKISAKKSAGAKATTKKVVDAKSDDKPAAKKPAPKKKTAAKDDKSK

Sequences:

>Translated_530_residues
MPSSLEKLSTNRVKLTIEMPFDELKPSLDKAYKDIANQVNVPGFRKGKVPAPVIDQRFGRGVVLQEAINDALPAAYGKAI
EENTVVPLGQPEIEVTKLEDGEVVEFTAEVDVRPDFDLPDVSAISVEVPAVEVPDEDVDERVETLRQRFATNTEVERAAA
KDDLVTIDLAGTRDGEVLEDATASGVTYKVGSEGMLEGLDEAVTGLKAGESAEFHSTLVGGPLRGQDADIKVTVTKVCEQ
ELPAVDDDFAQLVSQFDTVDEMRADLRTALENMARLDQAADARDKVLEEVISKIDIELPTNLIDSELEARRQQVNQQLAQ
AGMTVEEYLEDSEEEVDNADDFWAEIEKRSLDALKAQIVLDKMADDDEIGVEQNELTELLFRKAQQNGTSPEEEAQHMMQ
HNHLPDWMQEIRRGKALASMVGAATVTDSKGNALELDRIQPDGTIADKQAEEDAPAEDSAEKADGKATEESKAEEKAPAK
KATTKKISAKKSAGAKATTKKVVDAKSDDKPAAKKPAPKKKTAAKDDKSK
>Mature_529_residues
PSSLEKLSTNRVKLTIEMPFDELKPSLDKAYKDIANQVNVPGFRKGKVPAPVIDQRFGRGVVLQEAINDALPAAYGKAIE
ENTVVPLGQPEIEVTKLEDGEVVEFTAEVDVRPDFDLPDVSAISVEVPAVEVPDEDVDERVETLRQRFATNTEVERAAAK
DDLVTIDLAGTRDGEVLEDATASGVTYKVGSEGMLEGLDEAVTGLKAGESAEFHSTLVGGPLRGQDADIKVTVTKVCEQE
LPAVDDDFAQLVSQFDTVDEMRADLRTALENMARLDQAADARDKVLEEVISKIDIELPTNLIDSELEARRQQVNQQLAQA
GMTVEEYLEDSEEEVDNADDFWAEIEKRSLDALKAQIVLDKMADDDEIGVEQNELTELLFRKAQQNGTSPEEEAQHMMQH
NHLPDWMQEIRRGKALASMVGAATVTDSKGNALELDRIQPDGTIADKQAEEDAPAEDSAEKADGKATEESKAEEKAPAKK
ATTKKISAKKSAGAKATTKKVVDAKSDDKPAAKKPAPKKKTAAKDDKSK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=328, Percent_Identity=31.0975609756098, Blast_Score=148, Evalue=9e-37,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_PROAC (Q6A7E7)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056276.1
- HSSP:   P0A850
- ProteinModelPortal:   Q6A7E7
- GeneID:   2931945
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1575
- NMPDR:   fig|267747.1.peg.1553
- HOGENOM:   HBG635105
- OMA:   ETAINDG
- ProtClustDB:   PRK01490
- BioCyc:   PACN267747:PPA1575-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 57761; Mature: 57630

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSLEKLSTNRVKLTIEMPFDELKPSLDKAYKDIANQVNVPGFRKGKVPAPVIDQRFGR
CCCHHHHHCCCEEEEEEECCHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCC
GVVLQEAINDALPAAYGKAIEENTVVPLGQPEIEVTKLEDGEVVEFTAEVDVRPDFDLPD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCC
VSAISVEVPAVEVPDEDVDERVETLRQRFATNTEVERAAAKDDLVTIDLAGTRDGEVLED
CCEEEEECCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCCHHHH
ATASGVTYKVGSEGMLEGLDEAVTGLKAGESAEFHSTLVGGPLRGQDADIKVTVTKVCEQ
HCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEEHHHHHHH
ELPAVDDDFAQLVSQFDTVDEMRADLRTALENMARLDQAADARDKVLEEVISKIDIELPT
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCH
NLIDSELEARRQQVNQQLAQAGMTVEEYLEDSEEEVDNADDFWAEIEKRSLDALKAQIVL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DKMADDDEIGVEQNELTELLFRKAQQNGTSPEEEAQHMMQHNHLPDWMQEIRRGKALASM
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VGAATVTDSKGNALELDRIQPDGTIADKQAEEDAPAEDSAEKADGKATEESKAEEKAPAK
HHHHEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCHH
KATTKKISAKKSAGAKATTKKVVDAKSDDKPAAKKPAPKKKTAAKDDKSK
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PSSLEKLSTNRVKLTIEMPFDELKPSLDKAYKDIANQVNVPGFRKGKVPAPVIDQRFGR
CCHHHHHCCCEEEEEEECCHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCC
GVVLQEAINDALPAAYGKAIEENTVVPLGQPEIEVTKLEDGEVVEFTAEVDVRPDFDLPD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCC
VSAISVEVPAVEVPDEDVDERVETLRQRFATNTEVERAAAKDDLVTIDLAGTRDGEVLED
CCEEEEECCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCCHHHH
ATASGVTYKVGSEGMLEGLDEAVTGLKAGESAEFHSTLVGGPLRGQDADIKVTVTKVCEQ
HCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEEHHHHHHH
ELPAVDDDFAQLVSQFDTVDEMRADLRTALENMARLDQAADARDKVLEEVISKIDIELPT
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCH
NLIDSELEARRQQVNQQLAQAGMTVEEYLEDSEEEVDNADDFWAEIEKRSLDALKAQIVL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DKMADDDEIGVEQNELTELLFRKAQQNGTSPEEEAQHMMQHNHLPDWMQEIRRGKALASM
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VGAATVTDSKGNALELDRIQPDGTIADKQAEEDAPAEDSAEKADGKATEESKAEEKAPAK
HHHHEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCHH
KATTKKISAKKSAGAKATTKKVVDAKSDDKPAAKKPAPKKKTAAKDDKSK
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA