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The map label for this gene is malE [H]

Identifier: 50843027

GI number: 50843027

Start: 1669544

End: 1670809

Strand: Direct

Name: malE [H]

Synonym: PPA1552

Alternate gene names: 50843027

Gene position: 1669544-1670809 (Clockwise)

Preceding gene: 50843018

Following gene: 50843028

Centisome position: 65.21

GC content: 58.77

Gene sequence:

>1266_bases
ATGTCCGACATGACCCGTCGCTCAATGCTCCTTGGAAGCCTCAATGTTGCTGCCATCGGCGTCCTGTCCGCATGTGGTAG
CAACGACTCCCCCTCCACATCCGCGTCCTCGTCATCAGGTAAGAGCTCCTCCGCAGCTGGAGCCACCGGCTCAATCTTGG
TGTGGACCGACTCCAACCGTGAGCCCGTCTTGCGCAAAGTTGCCGAGCAGTTCAAATCCGACACCGGCGTCACCGTCAAA
CTAGCCGTCAAGGACTTCGCCAAGATTCCCGACGACTTCATCACCCAGGTGCCCACCAGCAAGGGCCCCGACGCCGCCAT
CGCCGCCCACGACGCCACCGGGCGCATGGTCCAAAATGGCGTTGTCGCCCCCTTGGAGCTCGGTGAAACCTCCGCCTATC
AGGACGTCGCCATCCAGGCGTTTACCGTCAACGGCAAGATCTACGGCATCCCCTACGCCACCGAGAACATCGCCCTAGTT
CGCAACACCAAATTCGTCAAGGACGCCCCAAAGACCTTCGACGACATGATCGAGATGGGCAAGAAGTCCGGGGCCAAGTA
CCCCTTCCTCGTCGGTTTGGATCCCAAGCAGTCAGATCCGTACCACCTGTACCCATTCCAGGCCTCCTTCGGGGCCCCAG
TCTTTGAGCTCAAGGACAAAGGATTCGATTCCAGCAAGGTCGCCATGGGAGGTACCAACGGCGAGAAGTTTGCCTCCTGG
CTAGCCGATCAAGGCAAGACCAAGAACTTCAACCTAAACGTGTCTCAAGACATATCGAAGGATCTGTTCGCTAAGGGCCA
GACAGCATTTATCCTCACCGGCCCCTGGAGCCTCGACAGTTTCACAAAGGCCGGCATCAAGTATGAGATCAGCGAGGTCC
CCTCAGCAGGTGATCGGCCCGCCACACCGTTCGTCGGCGTCCAGGGGTTCTGGATGAGCGCCAAGACTAAAAACGCCGTT
GCCACTCAAAAGTTCCTCGTCGAGTACATAGGAAACCCCGACGTCCAAGCCGAACTGTTCAAAGTTGGACACCGTCCGCC
GGCCAACAAGCAGGCTTTTGAGGAAGCCAAAACTGATAAAGACGTCGCCGCATTCGGTGCCGTCGGCCAAAAGGCCGTCC
CGATGCCAAACATCCCGGCCATGGGCTCAGTGTGGGCCGACTGGGGCGTTGCTCAGGCCGAGATTATCAGCGGCAAGGCC
TCTAGCCCGAAGGCCACCTGGGACGCAATGGTCAAGGCCATCGACGAAAAGATCAAGAAGGGCTGA

Upstream 100 bases:

>100_bases
AATCGCGTCAGCTTGCAAGGCAGTGACAACGAAATGTCCTCCTGATGCAAGAACGCTCGCTCGACGATGAGGGTCGAGCT
GCCTCGAAAGGTGTGACCCC

Downstream 100 bases:

>100_bases
TCGCAGCTATCTGGTGGGGCATACCGCATAGTATGCCCCACCCTCGCAACGCCAATGACGGCGTTTCCTATGACCCGTGA
AATCGGAGAATCCCCACATC

Product: putative periplasmic maltose-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSDMTRRSMLLGSLNVAAIGVLSACGSNDSPSTSASSSSGKSSSAAGATGSILVWTDSNREPVLRKVAEQFKSDTGVTVK
LAVKDFAKIPDDFITQVPTSKGPDAAIAAHDATGRMVQNGVVAPLELGETSAYQDVAIQAFTVNGKIYGIPYATENIALV
RNTKFVKDAPKTFDDMIEMGKKSGAKYPFLVGLDPKQSDPYHLYPFQASFGAPVFELKDKGFDSSKVAMGGTNGEKFASW
LADQGKTKNFNLNVSQDISKDLFAKGQTAFILTGPWSLDSFTKAGIKYEISEVPSAGDRPATPFVGVQGFWMSAKTKNAV
ATQKFLVEYIGNPDVQAELFKVGHRPPANKQAFEEAKTDKDVAAFGAVGQKAVPMPNIPAMGSVWADWGVAQAEIISGKA
SSPKATWDAMVKAIDEKIKKG

Sequences:

>Translated_421_residues
MSDMTRRSMLLGSLNVAAIGVLSACGSNDSPSTSASSSSGKSSSAAGATGSILVWTDSNREPVLRKVAEQFKSDTGVTVK
LAVKDFAKIPDDFITQVPTSKGPDAAIAAHDATGRMVQNGVVAPLELGETSAYQDVAIQAFTVNGKIYGIPYATENIALV
RNTKFVKDAPKTFDDMIEMGKKSGAKYPFLVGLDPKQSDPYHLYPFQASFGAPVFELKDKGFDSSKVAMGGTNGEKFASW
LADQGKTKNFNLNVSQDISKDLFAKGQTAFILTGPWSLDSFTKAGIKYEISEVPSAGDRPATPFVGVQGFWMSAKTKNAV
ATQKFLVEYIGNPDVQAELFKVGHRPPANKQAFEEAKTDKDVAAFGAVGQKAVPMPNIPAMGSVWADWGVAQAEIISGKA
SSPKATWDAMVKAIDEKIKKG
>Mature_420_residues
SDMTRRSMLLGSLNVAAIGVLSACGSNDSPSTSASSSSGKSSSAAGATGSILVWTDSNREPVLRKVAEQFKSDTGVTVKL
AVKDFAKIPDDFITQVPTSKGPDAAIAAHDATGRMVQNGVVAPLELGETSAYQDVAIQAFTVNGKIYGIPYATENIALVR
NTKFVKDAPKTFDDMIEMGKKSGAKYPFLVGLDPKQSDPYHLYPFQASFGAPVFELKDKGFDSSKVAMGGTNGEKFASWL
ADQGKTKNFNLNVSQDISKDLFAKGQTAFILTGPWSLDSFTKAGIKYEISEVPSAGDRPATPFVGVQGFWMSAKTKNAVA
TQKFLVEYIGNPDVQAELFKVGHRPPANKQAFEEAKTDKDVAAFGAVGQKAVPMPNIPAMGSVWADWGVAQAEIISGKAS
SPKATWDAMVKAIDEKIKKG

Specific function: Involved in the high-affinity maltose membrane transport system malEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=374, Percent_Identity=27.5401069518717, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44761; Mature: 44630

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMTRRSMLLGSLNVAAIGVLSACGSNDSPSTSASSSSGKSSSAAGATGSILVWTDSNR
CCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
EPVLRKVAEQFKSDTGVTVKLAVKDFAKIPDDFITQVPTSKGPDAAIAAHDATGRMVQNG
CHHHHHHHHHHCCCCCCEEEEEEHHHHHCCHHHHHHCCCCCCCCCEEEECCCCCHHHHCC
VVAPLELGETSAYQDVAIQAFTVNGKIYGIPYATENIALVRNTKFVKDAPKTFDDMIEMG
CCCEEECCCCCCCCEEEEEEEEECCEEEECCCCCCCEEEEECCCHHHCCHHHHHHHHHHH
KKSGAKYPFLVGLDPKQSDPYHLYPFQASFGAPVFELKDKGFDSSKVAMGGTNGEKFASW
HHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCEEEECCCCHHHHHHH
LADQGKTKNFNLNVSQDISKDLFAKGQTAFILTGPWSLDSFTKAGIKYEISEVPSAGDRP
HHHCCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCEEEHHHCCCCCCCC
ATPFVGVQGFWMSAKTKNAVATQKFLVEYIGNPDVQAELFKVGHRPPANKQAFEEAKTDK
CCCEECCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHCCCC
DVAAFGAVGQKAVPMPNIPAMGSVWADWGVAQAEIISGKASSPKATWDAMVKAIDEKIKK
HHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure 
SDMTRRSMLLGSLNVAAIGVLSACGSNDSPSTSASSSSGKSSSAAGATGSILVWTDSNR
CCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
EPVLRKVAEQFKSDTGVTVKLAVKDFAKIPDDFITQVPTSKGPDAAIAAHDATGRMVQNG
CHHHHHHHHHHCCCCCCEEEEEEHHHHHCCHHHHHHCCCCCCCCCEEEECCCCCHHHHCC
VVAPLELGETSAYQDVAIQAFTVNGKIYGIPYATENIALVRNTKFVKDAPKTFDDMIEMG
CCCEEECCCCCCCCEEEEEEEEECCEEEECCCCCCCEEEEECCCHHHCCHHHHHHHHHHH
KKSGAKYPFLVGLDPKQSDPYHLYPFQASFGAPVFELKDKGFDSSKVAMGGTNGEKFASW
HHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCEEEECCCCHHHHHHH
LADQGKTKNFNLNVSQDISKDLFAKGQTAFILTGPWSLDSFTKAGIKYEISEVPSAGDRP
HHHCCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCEEEHHHCCCCCCCC
ATPFVGVQGFWMSAKTKNAVATQKFLVEYIGNPDVQAELFKVGHRPPANKQAFEEAKTDK
CCCEECCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHCCCC
DVAAFGAVGQKAVPMPNIPAMGSVWADWGVAQAEIISGKASSPKATWDAMVKAIDEKIKK
HHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]