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The map label for this gene is myrA [H]

Identifier: 50843014

GI number: 50843014

Start: 1656042

End: 1656863

Strand: Direct

Name: myrA [H]

Synonym: PPA1536

Alternate gene names: 50843014

Gene position: 1656042-1656863 (Clockwise)

Preceding gene: 50843013

Following gene: 50843018

Centisome position: 64.68

GC content: 61.19

Gene sequence:

>822_bases
GTGACGCCGACACTTTCCGTAGTCCTGCCGTGGCTGATCTGTCCCACCTGTCAGCGGCGCGGCTGTGAAAACCCGTTGGC
CCGTGATGGGAGGTCTCTGCGATGCCCCGAGCACCACTGTCTCGATATCGCCAAGCAGGGCTACGTCACCATGACAAGCA
CCCCGGCCGGTGCTAATGCGGACACCGTCGAGATGATCGCTGCCCGCCAAAGGGTGTTGGACGCCGGGCTGTTCGGTGAC
CTTGATGCTCGGCTGGCCGAGATTCTCATCGGCCGAGATCGCATTGTGGAAGTAGGAGCTGGAACCGGTCACCATCTGGC
CCAGGTTCTCGAGGCCCACCCCGATTCCCACGGTCTGGCATCTGACGTCTCGAAAGCAGCTATCCGGCGGGCGGCGAAAG
CTCATCCTAGGATGGCGGCAGTGGTTGCCGATACCTGGGCTGGGCTGCCTATCCGCACTGGCTGTATAGATGCTGGGCTG
TGCGTCTTTGCGCCTCGCAACCGTGACGAGTTCGCGCGCATCATGAAACCTGACGGGATCTTCGTCGTCGTCACCCCGCT
ACCGGACCATTTGCAACAGCTGCGTGAACGCACCGGGATGATCGGCATCCAGCCCCACAAGCACGACGAGCTCATGGCAG
CTTTGGCATCACGGTTTACCGTTATCGGCCATGAAGAGGTGCGAGCGGACATTCCTCTGGACAAACAGACGGCCTCTGAC
GCTGTGGCAATGGGGCCTAGCGCCCACCACATCCACAACGTTCATGTCGATGCCATGTCCATCACGTCAGCTGTGGAAGT
GACGGTTCTGTCTCCCCGGTGA

Upstream 100 bases:

>100_bases
AAGCGGCTCGTTCAGCCGCTCAGCGAGCTGTCAGCGGCCCGGAACCGAATCGCCATGACGCGTCACACAACCGCGGCGCG
AAGACACCAGGGGCCCACAC

Downstream 100 bases:

>100_bases
GCGGGGTTCCTGCCGGAAAGAACCACCCTCAAAACTGGCGGTTATCGATGCTGTGCTCGGCCTTGTCTAGCCGACGACGC
GCCTTGCGTACCTTGCGATC

Product: putative resistance protein

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTPTLSVVLPWLICPTCQRRGCENPLARDGRSLRCPEHHCLDIAKQGYVTMTSTPAGANADTVEMIAARQRVLDAGLFGD
LDARLAEILIGRDRIVEVGAGTGHHLAQVLEAHPDSHGLASDVSKAAIRRAAKAHPRMAAVVADTWAGLPIRTGCIDAGL
CVFAPRNRDEFARIMKPDGIFVVVTPLPDHLQQLRERTGMIGIQPHKHDELMAALASRFTVIGHEEVRADIPLDKQTASD
AVAMGPSAHHIHNVHVDAMSITSAVEVTVLSPR

Sequences:

>Translated_273_residues
MTPTLSVVLPWLICPTCQRRGCENPLARDGRSLRCPEHHCLDIAKQGYVTMTSTPAGANADTVEMIAARQRVLDAGLFGD
LDARLAEILIGRDRIVEVGAGTGHHLAQVLEAHPDSHGLASDVSKAAIRRAAKAHPRMAAVVADTWAGLPIRTGCIDAGL
CVFAPRNRDEFARIMKPDGIFVVVTPLPDHLQQLRERTGMIGIQPHKHDELMAALASRFTVIGHEEVRADIPLDKQTASD
AVAMGPSAHHIHNVHVDAMSITSAVEVTVLSPR
>Mature_272_residues
TPTLSVVLPWLICPTCQRRGCENPLARDGRSLRCPEHHCLDIAKQGYVTMTSTPAGANADTVEMIAARQRVLDAGLFGDL
DARLAEILIGRDRIVEVGAGTGHHLAQVLEAHPDSHGLASDVSKAAIRRAAKAHPRMAAVVADTWAGLPIRTGCIDAGLC
VFAPRNRDEFARIMKPDGIFVVVTPLPDHLQQLRERTGMIGIQPHKHDELMAALASRFTVIGHEEVRADIPLDKQTASDA
VAMGPSAHHIHNVHVDAMSITSAVEVTVLSPR

Specific function: Confers strong resistance to mycinamicin (MM) and tylosin (TY). May function as methyltransferase [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=198, Percent_Identity=34.3434343434343, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 29435; Mature: 29304

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTLSVVLPWLICPTCQRRGCENPLARDGRSLRCPEHHCLDIAKQGYVTMTSTPAGANA
CCCHHHHHHHHHHCCHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCC
DTVEMIAARQRVLDAGLFGDLDARLAEILIGRDRIVEVGAGTGHHLAQVLEAHPDSHGLA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCHH
SDVSKAAIRRAAKAHPRMAAVVADTWAGLPIRTGCIDAGLCVFAPRNRDEFARIMKPDGI
HHHHHHHHHHHHHCCCHHEEEHHHHCCCCCEECCCCCCCEEEECCCCHHHHHHHCCCCCE
FVVVTPLPDHLQQLRERTGMIGIQPHKHDELMAALASRFTVIGHEEVRADIPLDKQTASD
EEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEECHHHHHCCCCCCCCCCCC
AVAMGPSAHHIHNVHVDAMSITSAVEVTVLSPR
HHHCCCCCHHEEEEEEHHHHHCCEEEEEEECCC
>Mature Secondary Structure 
TPTLSVVLPWLICPTCQRRGCENPLARDGRSLRCPEHHCLDIAKQGYVTMTSTPAGANA
CCHHHHHHHHHHCCHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCC
DTVEMIAARQRVLDAGLFGDLDARLAEILIGRDRIVEVGAGTGHHLAQVLEAHPDSHGLA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCHH
SDVSKAAIRRAAKAHPRMAAVVADTWAGLPIRTGCIDAGLCVFAPRNRDEFARIMKPDGI
HHHHHHHHHHHHHCCCHHEEEHHHHCCCCCEECCCCCCCEEEECCCCHHHHHHHCCCCCE
FVVVTPLPDHLQQLRERTGMIGIQPHKHDELMAALASRFTVIGHEEVRADIPLDKQTASD
EEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEECHHHHHCCCCCCCCCCCC
AVAMGPSAHHIHNVHVDAMSITSAVEVTVLSPR
HHHCCCCCHHEEEEEEHHHHHCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8163173 [H]