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The map label for this gene is def1 [H]

Identifier: 50843003

GI number: 50843003

Start: 1647164

End: 1647766

Strand: Direct

Name: def1 [H]

Synonym: PPA1525

Alternate gene names: 50843003

Gene position: 1647164-1647766 (Clockwise)

Preceding gene: 50843002

Following gene: 50843005

Centisome position: 64.34

GC content: 62.19

Gene sequence:

>603_bases
ATGCGCAAGCACACCCCCAATGACAACCCCCTTTGGGAGGATCTGTTCAAAGGTGGGTCTCTGCGTCGGGTGACACGATG
GGGAGAATCCGTCCTGCACGCGCAGACCCGTCCAGTCACAGAGTTCGATGAGGACCTGACGACCCTCATCCGCGACATGT
TCGCCACGATGAACGCCGCCGACGGTGTCGGGCTGGCCGCAACTCAAGTCGGGGTTGATTTGTCCTTGTTCGTCTACGCC
TGCCCTGACGCTAACAACATCGTCCACCACGGAGCATTCTGCAACCCAGTTGTCACCCTGCCGGAAGGACACGATCGCCA
CTTGGAAGCCGCCGATGAGGGCTGCTTGTCATGGCCCGGCGGTTTCCAGTCCATGGCTCGCCCCGATCTGGCCACCTGCA
CTGGTCAGGATCCGTGGGGCAACGACATCACCGTCACCGGAACCGGGTTCTTCGCCCGGTGTCTGCAACACGAAACCGAC
CACTGCAACGGCATCGTCTTCGGGGACCGACTCTCCAAGCGTGCCCGCCGCAAACTCGACGAGCAACACGACAGCGTGGC
GCACCTGTACCCCGACGACTGGCCCCTCACTCCAAAGGGCTGA

Upstream 100 bases:

>100_bases
CATGCGGGTCATCGTGGGCCCGACTACCGGATGATCACGGGCCGTCGTCTACTCGTGACACAATCAGCTACAAACATTTA
TCAACCCCAGGAGATCAGCC

Downstream 100 bases:

>100_bases
CCCGGGTTACCCCCGCAACTCGTAGCAGGCGACCGCAGCTGCCGCCGCAACGTTGAGAGAGTCCACGCCGCCTGCCATCG
GGATACGCACCACGACGTCG

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MRKHTPNDNPLWEDLFKGGSLRRVTRWGESVLHAQTRPVTEFDEDLTTLIRDMFATMNAADGVGLAATQVGVDLSLFVYA
CPDANNIVHHGAFCNPVVTLPEGHDRHLEAADEGCLSWPGGFQSMARPDLATCTGQDPWGNDITVTGTGFFARCLQHETD
HCNGIVFGDRLSKRARRKLDEQHDSVAHLYPDDWPLTPKG

Sequences:

>Translated_200_residues
MRKHTPNDNPLWEDLFKGGSLRRVTRWGESVLHAQTRPVTEFDEDLTTLIRDMFATMNAADGVGLAATQVGVDLSLFVYA
CPDANNIVHHGAFCNPVVTLPEGHDRHLEAADEGCLSWPGGFQSMARPDLATCTGQDPWGNDITVTGTGFFARCLQHETD
HCNGIVFGDRLSKRARRKLDEQHDSVAHLYPDDWPLTPKG
>Mature_200_residues
MRKHTPNDNPLWEDLFKGGSLRRVTRWGESVLHAQTRPVTEFDEDLTTLIRDMFATMNAADGVGLAATQVGVDLSLFVYA
CPDANNIVHHGAFCNPVVTLPEGHDRHLEAADEGCLSWPGGFQSMARPDLATCTGQDPWGNDITVTGTGFFARCLQHETD
HCNGIVFGDRLSKRARRKLDEQHDSVAHLYPDDWPLTPKG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=154, Percent_Identity=30.5194805194805, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22152; Mature: 22152

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKHTPNDNPLWEDLFKGGSLRRVTRWGESVLHAQTRPVTEFDEDLTTLIRDMFATMNAA
CCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DGVGLAATQVGVDLSLFVYACPDANNIVHHGAFCNPVVTLPEGHDRHLEAADEGCLSWPG
CCCCEEHHHHCCCEEEEEEECCCCCCCEECCCCCCCEEECCCCCCCCCCHHCCCCCCCCC
GFQSMARPDLATCTGQDPWGNDITVTGTGFFARCLQHETDHCNGIVFGDRLSKRARRKLD
CHHHHCCCCCEECCCCCCCCCCEEEECHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHH
EQHDSVAHLYPDDWPLTPKG
HHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MRKHTPNDNPLWEDLFKGGSLRRVTRWGESVLHAQTRPVTEFDEDLTTLIRDMFATMNAA
CCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DGVGLAATQVGVDLSLFVYACPDANNIVHHGAFCNPVVTLPEGHDRHLEAADEGCLSWPG
CCCCEEHHHHCCCEEEEEEECCCCCCCEECCCCCCCEEECCCCCCCCCCHHCCCCCCCCC
GFQSMARPDLATCTGQDPWGNDITVTGTGFFARCLQHETDHCNGIVFGDRLSKRARRKLD
CHHHHCCCCCEECCCCCCCCCCEEEECHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHH
EQHDSVAHLYPDDWPLTPKG
HHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]