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The map label for this gene is proS

Identifier: 50842977

GI number: 50842977

Start: 1618624

End: 1620381

Strand: Reverse

Name: proS

Synonym: PPA1499

Alternate gene names: 50842977

Gene position: 1620381-1618624 (Counterclockwise)

Preceding gene: 50842983

Following gene: 50842976

Centisome position: 63.29

GC content: 60.58

Gene sequence:

>1758_bases
GTGTTGACGCGCATGTCACAGCTGTTCGTCCGAACCCTGCGAGAAGACCCGGCCGATGCCGAGGTCCCTAGCCACCGCTG
GCTCGTCCGGGCTGGATATATCCGCCGGGTCGCTCCCGGCGTCTACTCGTGGCTCCCGCTCGGGCTCCGAGTGATGCGCA
GGATCGAGGCCATCTGCCGCGAAGAGATGGCTAGCATCGGGGCTCAGGAGGTGAGTTTCCCCGCGCTACTCGCTTCTGAC
CCTTACAAGGAGACCGGACGCTGGGTCGAGTACGGCGATAACCTCTTCCGTCTCAAAGACCGTAAGGGGGTGGACATGCT
GCTAGGACCGACCCATGAGGAGATGTTCACCCTCATGGTCAAGGACTTGTACTCCTCGTACAAGGACCTGCCACTGTGCC
TCTATCAGATTCAGAACAAGTATCGCGACGAGGCTCGTCCGCGTGCCGGCATCCTGCGCGGTCGTGAGTTCGTCATGATG
GATGCCTATTCCTTTGATGTCGCTCAGGAAGGGCTCGACGAGGCCTACCAGGCTCAACGCGACGCCTACATTCGCATTTT
CAACCGACTTGGATTCGACTACGCCATTGTCAAGGCCATGTCCGGCCCAATGGGTGGATCTCGATCCGAGGAGTTCCTAG
CCGTTGCAGCCAACGGTGAGGACACTTTCGTTCGTTCTTCCGGCGGTTACGCTGCCAATGTGGAGGCCGTCCGGATGGCC
GTTCCTGATCCGCTGCCCATCGACGGGTTGCCGGAGGCTGAGGTTGTTGATACCCCTGACGCCCGGACCATCGAGTCGCT
TGTCCGGATCGCCAATGAGGTGCGTCCGCGAGCGGACCGTCCGTGGACTGGGGCCGATACCCTGAAAAACGTTGTATTCA
TGGTGGCCCATCCTGACGGCACCCGCGAGCCATTAGCCATCGGGCTTCCTGGAGATCGTGACGTTGATGAGAAGCGACTG
GAAGCGGTTCTGGAGCCCGCGGTGGTTGAACCCTTCACAGAAGAGGACTTTGCTGCCAATCCGCAGTTGGTAAAGGGCTA
CATCGGGCCGGGAGCCCTCGGCGAGGATAACTCCTCAAAGGTGCGGTACCTCGTTGATCCCCGCGTCGTGACCGGGACGT
CATGGATTACCGGAGCTGACCAGAAGGATCGACATGTCTTCAATCTTGTAGCCGGTCGCGATTTCACCCCCGATGGCGTT
ATTGACGTCGCCGAGATTCGCGAAGGTGATCCGGCTCCTGACGGCAGTGGTGAACTCCACCTAGCCCGCGGCATCGAAAT
CGGCCACATCTTCCAGCTTGGTAAGAAATACGCTGATGCCCTTGGGCTCAAGGTGCTCGATCACAACGGCAAGCTGGTGA
CGGTCACCATGGGTTCTTATGGTCTGGGCGTCTCCCGCGCCGTGGCCGCTATCGCTGAGGGTACCTGTGACGAGAAGGGG
TTGTGTTGGCCACGTGAGGTTGCCCCCTACGATGTCCAGGTGCTCGCCACCGGCAAGGGAACGGAGATCCTTGACGAGGC
CACGCGCATTGCCAGTGAACTGGCCGAGGCTGGTCTCGAAGTCCTCCTAGACGACCGCAAGGCGTCCCCTGGAGTGAAGT
TTGCTGATAGCGAGATTCTCGGCATGCCAACCTCTGTGGTCGTCGGCCGGGGGCTCAAGGATGGCCTAGTCGAAGTTCGC
GACCGTAGTACGGGTGAGAACCGTCAGGTTAGCGTAGCCGAGGCTGTGTGCGCCGTCCTCAAGGAGGTGCGCGGGTGA

Upstream 100 bases:

>100_bases
CGTACGAGACGGTGGTGCCTGCCGCTAGGATTCGACATGGCCGTGGCCGGTCACGCCGAAGGGCGGCGGAGGGTCGAGCG
CGGTCGGAAAGGTACACGTC

Downstream 100 bases:

>100_bases
AAGCTGAAGTGGCGTTGATCGTTTAACTGACCGACATCTGCACGTTGCTGTATGATCGCCAACTGTTCTCGAAAACTGGC
GCGGAAGTGACCCAATAAAC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MLTRMSQLFVRTLREDPADAEVPSHRWLVRAGYIRRVAPGVYSWLPLGLRVMRRIEAICREEMASIGAQEVSFPALLASD
PYKETGRWVEYGDNLFRLKDRKGVDMLLGPTHEEMFTLMVKDLYSSYKDLPLCLYQIQNKYRDEARPRAGILRGREFVMM
DAYSFDVAQEGLDEAYQAQRDAYIRIFNRLGFDYAIVKAMSGPMGGSRSEEFLAVAANGEDTFVRSSGGYAANVEAVRMA
VPDPLPIDGLPEAEVVDTPDARTIESLVRIANEVRPRADRPWTGADTLKNVVFMVAHPDGTREPLAIGLPGDRDVDEKRL
EAVLEPAVVEPFTEEDFAANPQLVKGYIGPGALGEDNSSKVRYLVDPRVVTGTSWITGADQKDRHVFNLVAGRDFTPDGV
IDVAEIREGDPAPDGSGELHLARGIEIGHIFQLGKKYADALGLKVLDHNGKLVTVTMGSYGLGVSRAVAAIAEGTCDEKG
LCWPREVAPYDVQVLATGKGTEILDEATRIASELAEAGLEVLLDDRKASPGVKFADSEILGMPTSVVVGRGLKDGLVEVR
DRSTGENRQVSVAEAVCAVLKEVRG

Sequences:

>Translated_585_residues
MLTRMSQLFVRTLREDPADAEVPSHRWLVRAGYIRRVAPGVYSWLPLGLRVMRRIEAICREEMASIGAQEVSFPALLASD
PYKETGRWVEYGDNLFRLKDRKGVDMLLGPTHEEMFTLMVKDLYSSYKDLPLCLYQIQNKYRDEARPRAGILRGREFVMM
DAYSFDVAQEGLDEAYQAQRDAYIRIFNRLGFDYAIVKAMSGPMGGSRSEEFLAVAANGEDTFVRSSGGYAANVEAVRMA
VPDPLPIDGLPEAEVVDTPDARTIESLVRIANEVRPRADRPWTGADTLKNVVFMVAHPDGTREPLAIGLPGDRDVDEKRL
EAVLEPAVVEPFTEEDFAANPQLVKGYIGPGALGEDNSSKVRYLVDPRVVTGTSWITGADQKDRHVFNLVAGRDFTPDGV
IDVAEIREGDPAPDGSGELHLARGIEIGHIFQLGKKYADALGLKVLDHNGKLVTVTMGSYGLGVSRAVAAIAEGTCDEKG
LCWPREVAPYDVQVLATGKGTEILDEATRIASELAEAGLEVLLDDRKASPGVKFADSEILGMPTSVVVGRGLKDGLVEVR
DRSTGENRQVSVAEAVCAVLKEVRG
>Mature_585_residues
MLTRMSQLFVRTLREDPADAEVPSHRWLVRAGYIRRVAPGVYSWLPLGLRVMRRIEAICREEMASIGAQEVSFPALLASD
PYKETGRWVEYGDNLFRLKDRKGVDMLLGPTHEEMFTLMVKDLYSSYKDLPLCLYQIQNKYRDEARPRAGILRGREFVMM
DAYSFDVAQEGLDEAYQAQRDAYIRIFNRLGFDYAIVKAMSGPMGGSRSEEFLAVAANGEDTFVRSSGGYAANVEAVRMA
VPDPLPIDGLPEAEVVDTPDARTIESLVRIANEVRPRADRPWTGADTLKNVVFMVAHPDGTREPLAIGLPGDRDVDEKRL
EAVLEPAVVEPFTEEDFAANPQLVKGYIGPGALGEDNSSKVRYLVDPRVVTGTSWITGADQKDRHVFNLVAGRDFTPDGV
IDVAEIREGDPAPDGSGELHLARGIEIGHIFQLGKKYADALGLKVLDHNGKLVTVTMGSYGLGVSRAVAAIAEGTCDEKG
LCWPREVAPYDVQVLATGKGTEILDEATRIASELAEAGLEVLLDDRKASPGVKFADSEILGMPTSVVVGRGLKDGLVEVR
DRSTGENRQVSVAEAVCAVLKEVRG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=233, Percent_Identity=39.0557939914163, Blast_Score=176, Evalue=5e-44,
Organism=Escherichia coli, GI1786392, Length=590, Percent_Identity=42.0338983050847, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1788013, Length=175, Percent_Identity=26.2857142857143, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532348, Length=205, Percent_Identity=38.5365853658537, Blast_Score=131, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320931, Length=245, Percent_Identity=37.9591836734694, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24656200, Length=223, Percent_Identity=35.8744394618834, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PROAC (Q6A7L9)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056204.1
- ProteinModelPortal:   Q6A7L9
- SMR:   Q6A7L9
- GeneID:   2933044
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1499
- NMPDR:   fig|267747.1.peg.1481
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   PACN267747:PPA1499-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64113; Mature: 64113

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRMSQLFVRTLREDPADAEVPSHRWLVRAGYIRRVAPGVYSWLPLGLRVMRRIEAICR
CCCHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EEMASIGAQEVSFPALLASDPYKETGRWVEYGDNLFRLKDRKGVDMLLGPTHEEMFTLMV
HHHHHCCCHHCCCCHHHCCCCHHHHCCEEECCCCEEEEECCCCCCEEECCCHHHHHHHHH
KDLYSSYKDLPLCLYQIQNKYRDEARPRAGILRGREFVMMDAYSFDVAQEGLDEAYQAQR
HHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHH
DAYIRIFNRLGFDYAIVKAMSGPMGGSRSEEFLAVAANGEDTFVRSSGGYAANVEAVRMA
HHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEECCCCCEECCEEEEEE
VPDPLPIDGLPEAEVVDTPDARTIESLVRIANEVRPRADRPWTGADTLKNVVFMVAHPDG
CCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEEEEECCCC
TREPLAIGLPGDRDVDEKRLEAVLEPAVVEPFTEEDFAANPQLVKGYIGPGALGEDNSSK
CCCCEEEECCCCCCCCHHHHHHHHCCHHCCCCCCCCCCCCCHHEEEECCCCCCCCCCCCC
VRYLVDPRVVTGTSWITGADQKDRHVFNLVAGRDFTPDGVIDVAEIREGDPAPDGSGELH
EEEEECCEEEECCCEECCCCCCCCEEEEEECCCCCCCCCCEEHHHHCCCCCCCCCCCCEE
LARGIEIGHIFQLGKKYADALGLKVLDHNGKLVTVTMGSYGLGVSRAVAAIAEGTCDEKG
EECCCCHHHHHHHHHHHHHHHCEEEEECCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCC
LCWPREVAPYDVQVLATGKGTEILDEATRIASELAEAGLEVLLDDRKASPGVKFADSEIL
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCEECCCCCC
GMPTSVVVGRGLKDGLVEVRDRSTGENRQVSVAEAVCAVLKEVRG
CCCHHHHHCCCCHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTRMSQLFVRTLREDPADAEVPSHRWLVRAGYIRRVAPGVYSWLPLGLRVMRRIEAICR
CCCHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EEMASIGAQEVSFPALLASDPYKETGRWVEYGDNLFRLKDRKGVDMLLGPTHEEMFTLMV
HHHHHCCCHHCCCCHHHCCCCHHHHCCEEECCCCEEEEECCCCCCEEECCCHHHHHHHHH
KDLYSSYKDLPLCLYQIQNKYRDEARPRAGILRGREFVMMDAYSFDVAQEGLDEAYQAQR
HHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHH
DAYIRIFNRLGFDYAIVKAMSGPMGGSRSEEFLAVAANGEDTFVRSSGGYAANVEAVRMA
HHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEECCCCCEECCEEEEEE
VPDPLPIDGLPEAEVVDTPDARTIESLVRIANEVRPRADRPWTGADTLKNVVFMVAHPDG
CCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEEEEECCCC
TREPLAIGLPGDRDVDEKRLEAVLEPAVVEPFTEEDFAANPQLVKGYIGPGALGEDNSSK
CCCCEEEECCCCCCCCHHHHHHHHCCHHCCCCCCCCCCCCCHHEEEECCCCCCCCCCCCC
VRYLVDPRVVTGTSWITGADQKDRHVFNLVAGRDFTPDGVIDVAEIREGDPAPDGSGELH
EEEEECCEEEECCCEECCCCCCCCEEEEEECCCCCCCCCCEEHHHHCCCCCCCCCCCCEE
LARGIEIGHIFQLGKKYADALGLKVLDHNGKLVTVTMGSYGLGVSRAVAAIAEGTCDEKG
EECCCCHHHHHHHHHHHHHHHCEEEEECCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCC
LCWPREVAPYDVQVLATGKGTEILDEATRIASELAEAGLEVLLDDRKASPGVKFADSEIL
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCEECCCCCC
GMPTSVVVGRGLKDGLVEVRDRSTGENRQVSVAEAVCAVLKEVRG
CCCHHHHHCCCCHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA