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The map label for this gene is tyrS

Identifier: 50842886

GI number: 50842886

Start: 1525985

End: 1527241

Strand: Reverse

Name: tyrS

Synonym: PPA1404

Alternate gene names: 50842886

Gene position: 1527241-1525985 (Counterclockwise)

Preceding gene: 50842887

Following gene: 50842885

Centisome position: 59.65

GC content: 57.76

Gene sequence:

>1257_bases
GTGTCGGATCTGTTGGACGACCTGGAGTGGAGGGGCCTCGTCGCTGACTCCACCGACCGTGAGGCGTTGGAGGCTCACCT
CGCCCAAGGACCCGTCACCTTCTATGTGGGCTTTGATCCGACCGCCAAAAGCCTCCATATTGGCCATCTTGTCCAGTTGA
TGCTGGTGCGAGCCCTCCAGGAACGCGGCCATAAGCCGTTGCTCCTCGTCGGTGGGGCGACCGGTCTTATTGGTGATCCT
AAGATGACCGGCGAGCGACATATGAACGATCGGGAAGTCGTTGCTGAGTGGGTGGAGTCGATCAAGGACCAGGTGAGCAA
GTATGTCGATACTGACGGCCGGAATGCTGCTCAGATCGTCAATAATTACGACTGGACGGTTAAGTACACAGCCCTAGACT
TGCTGCGCGACGTCGGCAAGCATTTCTCAGTCAACCGTATGCTCGCCCGCGACGTCGTTGCCCGACGACTAGAAAGCGGT
ATCTCATACACGGAGTTCAGCTACGTCCTGTTGCAGTCGCTGGACTTCTACGAACTTCATAAACGCTATGGCTGTACGTT
ACAAACGGGTGCTCAGGATCAGTGGGGAAACATCACTGCCGGTGCTGAGTTCATTCGCAAGACGACCGGTGACGTTGTCC
ATGGGTTGGTGACTCCGTTGATTACTAAGGCCGACGGTACCAAGTACGGCAAGACGGAGTCTGGGACGGTATGGGTGGAT
CCTGAGTTGACGAGCGCCTATGCCTTTCATCAATTCTTCCTCAACGCGGAGGACTCAAAGGTCATTGAGTACCTCAAAAT
CTTTAGCCCTCGTTCTCGGGAGGAGATTGAGGATCTTGCCCGTAAGACTGAGGAGGAACCGTGGCGTAGAGCGGCTCAGC
ATTGCCTTGCGGACGACCTGACCGATCTGGTGCATGGTGTGGAGCAGCGCAAGGCTGCCGAGGCTGCAGCCCAGGCCATC
TTCGGACGTGGCGAGTTGCGCGACCTCGATGAGCGGACGGTGCTCTCTCTGTCTGACGAGCTCGGTGCCGCCCATCATGA
AGGTGGCGAGTACCCGACAGTGGTTGGCGCGATGGTTGCCGCTGGTGTGGTTGACACCAAGTCTGGGGGCAGGCGTGCTG
TCGCCGAGGGTGGGGCTTACCTCAACAATGTTAAAGTCGCCGATCCTGATCAGCGTCTGACTGACGACGACTTCCTATGT
GGCCGTGTCGCCCTGGTCCGCCGTGGAAAGAAGACTGTGGGTGCTCTGACTCGCTGA

Upstream 100 bases:

>100_bases
GTGAGGAATTTGCGTGAACACACAGACTGTCTGGTCGGGCCCATCGCTAGACTTATAGTCAGTCGGCTTCGCACGAGGTC
GTTGACGAGAGGAAAATGAC

Downstream 100 bases:

>100_bases
CCTGTCCTGATCATGATCCGTCCGGGGGCCGCTAGTTGGGCGGTCCCCGGACGATTTTGTGTACCCGCAGATGCGTGCCA
GACAAGCGCCTCGGCTGTTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSDLLDDLEWRGLVADSTDREALEAHLAQGPVTFYVGFDPTAKSLHIGHLVQLMLVRALQERGHKPLLLVGGATGLIGDP
KMTGERHMNDREVVAEWVESIKDQVSKYVDTDGRNAAQIVNNYDWTVKYTALDLLRDVGKHFSVNRMLARDVVARRLESG
ISYTEFSYVLLQSLDFYELHKRYGCTLQTGAQDQWGNITAGAEFIRKTTGDVVHGLVTPLITKADGTKYGKTESGTVWVD
PELTSAYAFHQFFLNAEDSKVIEYLKIFSPRSREEIEDLARKTEEEPWRRAAQHCLADDLTDLVHGVEQRKAAEAAAQAI
FGRGELRDLDERTVLSLSDELGAAHHEGGEYPTVVGAMVAAGVVDTKSGGRRAVAEGGAYLNNVKVADPDQRLTDDDFLC
GRVALVRRGKKTVGALTR

Sequences:

>Translated_418_residues
MSDLLDDLEWRGLVADSTDREALEAHLAQGPVTFYVGFDPTAKSLHIGHLVQLMLVRALQERGHKPLLLVGGATGLIGDP
KMTGERHMNDREVVAEWVESIKDQVSKYVDTDGRNAAQIVNNYDWTVKYTALDLLRDVGKHFSVNRMLARDVVARRLESG
ISYTEFSYVLLQSLDFYELHKRYGCTLQTGAQDQWGNITAGAEFIRKTTGDVVHGLVTPLITKADGTKYGKTESGTVWVD
PELTSAYAFHQFFLNAEDSKVIEYLKIFSPRSREEIEDLARKTEEEPWRRAAQHCLADDLTDLVHGVEQRKAAEAAAQAI
FGRGELRDLDERTVLSLSDELGAAHHEGGEYPTVVGAMVAAGVVDTKSGGRRAVAEGGAYLNNVKVADPDQRLTDDDFLC
GRVALVRRGKKTVGALTR
>Mature_417_residues
SDLLDDLEWRGLVADSTDREALEAHLAQGPVTFYVGFDPTAKSLHIGHLVQLMLVRALQERGHKPLLLVGGATGLIGDPK
MTGERHMNDREVVAEWVESIKDQVSKYVDTDGRNAAQIVNNYDWTVKYTALDLLRDVGKHFSVNRMLARDVVARRLESGI
SYTEFSYVLLQSLDFYELHKRYGCTLQTGAQDQWGNITAGAEFIRKTTGDVVHGLVTPLITKADGTKYGKTESGTVWVDP
ELTSAYAFHQFFLNAEDSKVIEYLKIFSPRSREEIEDLARKTEEEPWRRAAQHCLADDLTDLVHGVEQRKAAEAAAQAIF
GRGELRDLDERTVLSLSDELGAAHHEGGEYPTVVGAMVAAGVVDTKSGGRRAVAEGGAYLNNVKVADPDQRLTDDDFLCG
RVALVRRGKKTVGALTR

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=399, Percent_Identity=35.5889724310777, Blast_Score=211, Evalue=8e-55,
Organism=Escherichia coli, GI1787925, Length=416, Percent_Identity=46.1538461538462, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25145802, Length=346, Percent_Identity=36.7052023121387, Blast_Score=198, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI25145805, Length=243, Percent_Identity=36.2139917695473, Blast_Score=151, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=32.4200913242009, Blast_Score=230, Evalue=3e-61,
Organism=Drosophila melanogaster, GI20130383, Length=430, Percent_Identity=34.1860465116279, Blast_Score=228, Evalue=6e-60,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_PROAC (Q6A7W0)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056113.1
- ProteinModelPortal:   Q6A7W0
- SMR:   Q6A7W0
- GeneID:   2931416
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1404
- NMPDR:   fig|267747.1.peg.1390
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   CLSK2763957
- BioCyc:   PACN267747:PPA1404-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46258; Mature: 46126

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 35-35 BINDING 168-168 BINDING 172-172 BINDING 231-231

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLDDLEWRGLVADSTDREALEAHLAQGPVTFYVGFDPTAKSLHIGHLVQLMLVRALQ
CCHHHHHHHHCCEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
ERGHKPLLLVGGATGLIGDPKMTGERHMNDREVVAEWVESIKDQVSKYVDTDGRNAAQIV
HCCCCCEEEECCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
NNYDWTVKYTALDLLRDVGKHFSVNRMLARDVVARRLESGISYTEFSYVLLQSLDFYELH
CCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHH
KRYGCTLQTGAQDQWGNITAGAEFIRKTTGDVVHGLVTPLITKADGTKYGKTESGTVWVD
HHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
PELTSAYAFHQFFLNAEDSKVIEYLKIFSPRSREEIEDLARKTEEEPWRRAAQHCLADDL
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TDLVHGVEQRKAAEAAAQAIFGRGELRDLDERTVLSLSDELGAAHHEGGEYPTVVGAMVA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHCCCCCCHHHHHHHHH
AGVVDTKSGGRRAVAEGGAYLNNVKVADPDQRLTDDDFLCGRVALVRRGKKTVGALTR
HCCCCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure 
SDLLDDLEWRGLVADSTDREALEAHLAQGPVTFYVGFDPTAKSLHIGHLVQLMLVRALQ
CHHHHHHHHCCEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
ERGHKPLLLVGGATGLIGDPKMTGERHMNDREVVAEWVESIKDQVSKYVDTDGRNAAQIV
HCCCCCEEEECCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
NNYDWTVKYTALDLLRDVGKHFSVNRMLARDVVARRLESGISYTEFSYVLLQSLDFYELH
CCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHH
KRYGCTLQTGAQDQWGNITAGAEFIRKTTGDVVHGLVTPLITKADGTKYGKTESGTVWVD
HHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
PELTSAYAFHQFFLNAEDSKVIEYLKIFSPRSREEIEDLARKTEEEPWRRAAQHCLADDL
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TDLVHGVEQRKAAEAAAQAIFGRGELRDLDERTVLSLSDELGAAHHEGGEYPTVVGAMVA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHCCCCCCHHHHHHHHH
AGVVDTKSGGRRAVAEGGAYLNNVKVADPDQRLTDDDFLCGRVALVRRGKKTVGALTR
HCCCCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA