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The map label for this gene is hisF

Identifier: 50842858

GI number: 50842858

Start: 1496412

End: 1497167

Strand: Reverse

Name: hisF

Synonym: PPA1376

Alternate gene names: 50842858

Gene position: 1497167-1496412 (Counterclockwise)

Preceding gene: 50842859

Following gene: 50842857

Centisome position: 58.48

GC content: 61.11

Gene sequence:

>756_bases
GTGGCTATACGTGTCATTCCATGTCTGGACGTTAAGGATGGGCGGGTCGTTAAAGGGGTTAACTTCGTCGGTCTGCGCGA
TGCCGGTGATCCCGTGGAGCTAGCGGCGGAATACGGGCGGCTCGGTGCCGACGAGGTGACTTTCCTCGACATTTCTGCCT
CAACAGAGGGGCGCGCCACCACTCGCGAGATGGTGACACGGTGCGCTGAGACAGTTTTCGTGCCCCTCACTGTAGGTGGT
GGGGTGCGCGGGGTCGACGACGTCGATGTCCTGCTGAGGGCGGGAGCTGACAAAGTTGGTGTCAACACCGCAGCGATTGC
TCATCCCGGGATGATCGACGAGGTCGCTGACCGGTTTGGCAATCAGGTCATCGTGTTGTCTGTCGATGCCCGTCGCGAAC
CAGATCGGCCATCCGGTTTTGGGGTGACGACCCATGGTGGCACCCGTTCGGCAGGGTTGGACGCCGTTGAGTGGGCTTGC
CAAGCCGTGGAGCGTGGGGCAGGTGAGATTCTGCTCAATTCGATGGACGCCGATGGTACGGCTGATGGCTTCGATATTGA
GATGATCGAGGCAGTGAGAGCAGCTGTCGACGTCCCCCTCATCGCTTCAGGTGGAGCTGGAAAGGTTGCCGATTTCGTCG
AGGCGGCGCGCGCCGGAGTTGACGCGGTGCTCGCCGCGTCAGTTTTTCACTATCACACCCTGACGATCGCGCAGATCAAG
GACGGCCTGCGTCATGCCGGCTTTGAGGTCCGCTGA

Upstream 100 bases:

>100_bases
GCCATGCAGCGTGGGGTTGTCACCATGGACCGCGAAGAAGACTGATTCGTGCGCCCACTAACAAGGGGGATGTGCGGCCC
GGTAGCCTGAGGTCATGGCT

Downstream 100 bases:

>100_bases
GCGAACACGTACTGCACGCCTAGCCGATGTGGTAGTCATAACTTATGGACTGCCTTTTCTGCTCAATCGCTGCGGGAGAC
ATCCCAGCGACGATCATTGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MAIRVIPCLDVKDGRVVKGVNFVGLRDAGDPVELAAEYGRLGADEVTFLDISASTEGRATTREMVTRCAETVFVPLTVGG
GVRGVDDVDVLLRAGADKVGVNTAAIAHPGMIDEVADRFGNQVIVLSVDARREPDRPSGFGVTTHGGTRSAGLDAVEWAC
QAVERGAGEILLNSMDADGTADGFDIEMIEAVRAAVDVPLIASGGAGKVADFVEAARAGVDAVLAASVFHYHTLTIAQIK
DGLRHAGFEVR

Sequences:

>Translated_251_residues
MAIRVIPCLDVKDGRVVKGVNFVGLRDAGDPVELAAEYGRLGADEVTFLDISASTEGRATTREMVTRCAETVFVPLTVGG
GVRGVDDVDVLLRAGADKVGVNTAAIAHPGMIDEVADRFGNQVIVLSVDARREPDRPSGFGVTTHGGTRSAGLDAVEWAC
QAVERGAGEILLNSMDADGTADGFDIEMIEAVRAAVDVPLIASGGAGKVADFVEAARAGVDAVLAASVFHYHTLTIAQIK
DGLRHAGFEVR
>Mature_250_residues
AIRVIPCLDVKDGRVVKGVNFVGLRDAGDPVELAAEYGRLGADEVTFLDISASTEGRATTREMVTRCAETVFVPLTVGGG
VRGVDDVDVLLRAGADKVGVNTAAIAHPGMIDEVADRFGNQVIVLSVDARREPDRPSGFGVTTHGGTRSAGLDAVEWACQ
AVERGAGEILLNSMDADGTADGFDIEMIEAVRAAVDVPLIASGGAGKVADFVEAARAGVDAVLAASVFHYHTLTIAQIKD
GLRHAGFEVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=43.75, Blast_Score=211, Evalue=3e-56,
Organism=Escherichia coli, GI87082028, Length=238, Percent_Identity=27.7310924369748, Blast_Score=78, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=312, Percent_Identity=35.8974358974359, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_PROAC (Q6A7Y8)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056085.1
- ProteinModelPortal:   Q6A7Y8
- SMR:   Q6A7Y8
- GeneID:   2931448
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1376
- NMPDR:   fig|267747.1.peg.1362
- HOGENOM:   HBG541613
- OMA:   ARWEIFT
- ProtClustDB:   CLSK2763931
- BioCyc:   PACN267747:PPA1376-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26141; Mature: 26010

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRVIPCLDVKDGRVVKGVNFVGLRDAGDPVELAAEYGRLGADEVTFLDISASTEGRAT
CEEEEEEEECCCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHH
TREMVTRCAETVFVPLTVGGGVRGVDDVDVLLRAGADKVGVNTAAIAHPGMIDEVADRFG
HHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHC
NQVIVLSVDARREPDRPSGFGVTTHGGTRSAGLDAVEWACQAVERGAGEILLNSMDADGT
CEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
ADGFDIEMIEAVRAAVDVPLIASGGAGKVADFVEAARAGVDAVLAASVFHYHTLTIAQIK
CCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
DGLRHAGFEVR
HHHHHCCCCCC
>Mature Secondary Structure 
AIRVIPCLDVKDGRVVKGVNFVGLRDAGDPVELAAEYGRLGADEVTFLDISASTEGRAT
EEEEEEEECCCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHH
TREMVTRCAETVFVPLTVGGGVRGVDDVDVLLRAGADKVGVNTAAIAHPGMIDEVADRFG
HHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHC
NQVIVLSVDARREPDRPSGFGVTTHGGTRSAGLDAVEWACQAVERGAGEILLNSMDADGT
CEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
ADGFDIEMIEAVRAAVDVPLIASGGAGKVADFVEAARAGVDAVLAASVFHYHTLTIAQIK
CCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
DGLRHAGFEVR
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA