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The map label for this gene is ilvE [H]

Identifier: 50842852

GI number: 50842852

Start: 1491137

End: 1492228

Strand: Reverse

Name: ilvE [H]

Synonym: PPA1368

Alternate gene names: 50842852

Gene position: 1492228-1491137 (Counterclockwise)

Preceding gene: 50842854

Following gene: 50842850

Centisome position: 58.28

GC content: 59.62

Gene sequence:

>1092_bases
ATGAGTTTGCGCTTTGCTTCTGCTGACGATCTGACGTGGTCCTCCGACGAGCGGATCGCGCTGGAACATGCCAATCCACG
ATTTGGTGCGGTCCTTGCCGACCATATGGCTGTTGCTACCTGGCAGGCCGGTGAAGGCTGGGGAGACGACGCCGTGGTCA
ACTACCACGGACTTGATATCAATCCTGGCAGCGCTGTGCTGCATTATGCCCAGGAGATTTTCGAGGGCCTGAAGGCTTAC
CGCCACGCTGACGGTTCTATCTGGCTCTTCCGCCCTGATCAGAGCGGTGAGCGTTTTGTTCGGTCGGCCGAGCGTCTCGC
CCTGCCCACGCTGCGAGTCGAAGACTTCGTTACTGCTTGTATGCGCTTGGTTGAGGTCGACTCCCGTTGGGTCCCTGAGC
CCGGTGAGGGTGGAGAGAAGTCCCTCTACCTGCGGCCGTTCATGATCGCCTACCAGGATTTCCTCGGTCTTGCCGCGGCA
AGTACCGTGCTGTTTTCCGTTATTGGTTCTCCCGTTGGCGCTTACTTTGTCAGCGGGGTGAAACCGCTGCGGCTCCTCGT
CGAGCGTCGTCAAGCTCGAACGGCCCCCGGCGGTACCGGTGAGGCGAAGTGCGGTGGCAATTACGCAGCTTCGCTGCGTT
CCCAGATCGATGCCAAGACCCGTGGCTGCAACGAGGTGCTCTTTGTCGATGCAGTTGAACATCGCTGGATCGAGGAACTG
GGTGGTATGAACTTCATGGCCATCAGCAAGGACGGTCAGCTCGTCACCCCCGAGTTAGCTGGCACCATCCTACGTGGCGT
GACTCGCAGATCCATTCTGGAAGTTGCCCCCGACCTCGGTCTTGAACCAGTGGAGCGCAAGATCGATGTTGACGAGCTCC
TTGATGGCGTTCGCTCTGGCGAGTTCCCGGAAGTCTTCGCCTGCGGTACCGCCGCGGTTGTCACACCGATCGGCTCTTTC
CTAGATGGAGATACCGACGTGAAGGTCTCTGAGCCCACCGGAAAGACCACGATGGAGATCCGTCGCCGTCTGCTGGATAT
CCAGTTCGGACGCGCTGAGGACACCCGTGGCTGGTTGAAGCGAGTCTGCTGA

Upstream 100 bases:

>100_bases
CGTCGTTGGTCATAGGTGAACCATACTCAGCACGTCCACGATATGATCCGTCGATTCAAAAAATGGAACGAATGGCTCGG
CGTGGGTGTAATGTGTGGCT

Downstream 100 bases:

>100_bases
CGGCGTCGACGTCCATTGGGGCCGGCCCCAATGATATGTTCACGACTGGGCTACGACGGTGTCGATGACAATGTCTTGCG
GCTGGAAGGTTTGCCCGACG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSLRFASADDLTWSSDERIALEHANPRFGAVLADHMAVATWQAGEGWGDDAVVNYHGLDINPGSAVLHYAQEIFEGLKAY
RHADGSIWLFRPDQSGERFVRSAERLALPTLRVEDFVTACMRLVEVDSRWVPEPGEGGEKSLYLRPFMIAYQDFLGLAAA
STVLFSVIGSPVGAYFVSGVKPLRLLVERRQARTAPGGTGEAKCGGNYAASLRSQIDAKTRGCNEVLFVDAVEHRWIEEL
GGMNFMAISKDGQLVTPELAGTILRGVTRRSILEVAPDLGLEPVERKIDVDELLDGVRSGEFPEVFACGTAAVVTPIGSF
LDGDTDVKVSEPTGKTTMEIRRRLLDIQFGRAEDTRGWLKRVC

Sequences:

>Translated_363_residues
MSLRFASADDLTWSSDERIALEHANPRFGAVLADHMAVATWQAGEGWGDDAVVNYHGLDINPGSAVLHYAQEIFEGLKAY
RHADGSIWLFRPDQSGERFVRSAERLALPTLRVEDFVTACMRLVEVDSRWVPEPGEGGEKSLYLRPFMIAYQDFLGLAAA
STVLFSVIGSPVGAYFVSGVKPLRLLVERRQARTAPGGTGEAKCGGNYAASLRSQIDAKTRGCNEVLFVDAVEHRWIEEL
GGMNFMAISKDGQLVTPELAGTILRGVTRRSILEVAPDLGLEPVERKIDVDELLDGVRSGEFPEVFACGTAAVVTPIGSF
LDGDTDVKVSEPTGKTTMEIRRRLLDIQFGRAEDTRGWLKRVC
>Mature_362_residues
SLRFASADDLTWSSDERIALEHANPRFGAVLADHMAVATWQAGEGWGDDAVVNYHGLDINPGSAVLHYAQEIFEGLKAYR
HADGSIWLFRPDQSGERFVRSAERLALPTLRVEDFVTACMRLVEVDSRWVPEPGEGGEKSLYLRPFMIAYQDFLGLAAAS
TVLFSVIGSPVGAYFVSGVKPLRLLVERRQARTAPGGTGEAKCGGNYAASLRSQIDAKTRGCNEVLFVDAVEHRWIEELG
GMNFMAISKDGQLVTPELAGTILRGVTRRSILEVAPDLGLEPVERKIDVDELLDGVRSGEFPEVFACGTAAVVTPIGSFL
DGDTDVKVSEPTGKTTMEIRRRLLDIQFGRAEDTRGWLKRVC

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=337, Percent_Identity=39.4658753709199, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI38176287, Length=337, Percent_Identity=39.4658753709199, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI296010904, Length=337, Percent_Identity=39.4658753709199, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI50658084, Length=331, Percent_Identity=37.1601208459215, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI258614015, Length=287, Percent_Identity=37.6306620209059, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI296010902, Length=337, Percent_Identity=34.1246290801187, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI296010900, Length=337, Percent_Identity=34.1246290801187, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI48994963, Length=334, Percent_Identity=27.8443113772455, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17568601, Length=356, Percent_Identity=35.6741573033708, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI17565728, Length=357, Percent_Identity=34.733893557423, Blast_Score=178, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6322002, Length=350, Percent_Identity=39.1428571428571, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6322608, Length=340, Percent_Identity=37.9411764705882, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24641779, Length=362, Percent_Identity=36.4640883977901, Blast_Score=204, Evalue=7e-53,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39776; Mature: 39644

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRFASADDLTWSSDERIALEHANPRFGAVLADHMAVATWQAGEGWGDDAVVNYHGLDI
CCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHEEEEECCCCCCCCCEEEEECCCC
NPGSAVLHYAQEIFEGLKAYRHADGSIWLFRPDQSGERFVRSAERLALPTLRVEDFVTAC
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
MRLVEVDSRWVPEPGEGGEKSLYLRPFMIAYQDFLGLAAASTVLFSVIGSPVGAYFVSGV
HHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
KPLRLLVERRQARTAPGGTGEAKCGGNYAASLRSQIDAKTRGCNEVLFVDAVEHRWIEEL
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
GGMNFMAISKDGQLVTPELAGTILRGVTRRSILEVAPDLGLEPVERKIDVDELLDGVRSG
CCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCC
EFPEVFACGTAAVVTPIGSFLDGDTDVKVSEPTGKTTMEIRRRLLDIQFGRAEDTRGWLK
CCCCEEECCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
RVC
HCC
>Mature Secondary Structure 
SLRFASADDLTWSSDERIALEHANPRFGAVLADHMAVATWQAGEGWGDDAVVNYHGLDI
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHEEEEECCCCCCCCCEEEEECCCC
NPGSAVLHYAQEIFEGLKAYRHADGSIWLFRPDQSGERFVRSAERLALPTLRVEDFVTAC
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
MRLVEVDSRWVPEPGEGGEKSLYLRPFMIAYQDFLGLAAASTVLFSVIGSPVGAYFVSGV
HHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
KPLRLLVERRQARTAPGGTGEAKCGGNYAASLRSQIDAKTRGCNEVLFVDAVEHRWIEEL
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
GGMNFMAISKDGQLVTPELAGTILRGVTRRSILEVAPDLGLEPVERKIDVDELLDGVRSG
CCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCC
EFPEVFACGTAAVVTPIGSFLDGDTDVKVSEPTGKTTMEIRRRLLDIQFGRAEDTRGWLK
CCCCEEECCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
RVC
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]