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The map label for this gene is thrB

Identifier: 50842734

GI number: 50842734

Start: 1359690

End: 1360580

Strand: Reverse

Name: thrB

Synonym: PPA1256

Alternate gene names: 50842734

Gene position: 1360580-1359690 (Counterclockwise)

Preceding gene: 50842735

Following gene: 50842733

Centisome position: 53.14

GC content: 61.28

Gene sequence:

>891_bases
ATGGACAACAGCGTGCGGGTGCGTGTTCCCGCTACTTCTGCGAACCTCGGTCCCGGTTATGACTGTATCGGCTTAGCTCT
GGATCTATGGGATGAGGTTAGTGTCGGGGTACTCGATCGTCCCGGGGTGATGATCGACGTGACAGGGGAAGGGGCTGACA
CGGTTCCCCATGACGAATCCCACCTCGTGATGGCGACGTTACGCCAGGGTTTGGTGGAGCTGGGCTATCCCCACCCTGAT
GCTGGGTTGCATCTGACCGCTATCAACTCCATTCCGCAAAGCCGAGGTCTGGGATCGTCGGCTGCGGCCGTCGTGTCCGG
GTTGGCATTGGCTTGGGGGCTGGCCCGCCCTGGGTTCCCGCTGGATCGCAGTGCGCTTCTGACGATGGCTGCCGCTATCG
AGGGTCATCCGGACAATGCCGCTCCCGCGATCCTCGGGGGTGCGCAGTTGGCGTGGCTCGATGGCGAGGCCGTCAACCAC
ATCGGCTTGACTGTCAACCCGTCCATTGTGTTTCGGGTATATGTTCCCGATCGCCTCGTACCAACGGCCTTGGCCCGTCA
GGTGCTTCCAGAACAGGTCGACCGCGTTGACGCGGTCCATCAGGTCCTGGCGGCGTCACTGCTTGTCACTGCCCTGACAA
CCTCACCTGAACATCTCTTGGCGGCAACTCAGGACTGGATCCACCAACCATACCGGCGCGCTCTTATGCCGGAATCGGCA
GCGCTCACGGATAGATTGCGCGGACGTGGCGTCGCCACTGTCATTTCTGGCGCTGGGCCTACCGTTCTGGCCCTGGGGAG
CCGTGACCAACTCGAGAAGGTGTCTGACGTCGATACTGCCGGGTTCGTGGCACATGACCTAGTTCTTGGTGAAGGGGTCC
ATTTCTTCTGA

Upstream 100 bases:

>100_bases
GACACTGCATTGAGTCGCACTACCTTGTCAACCGAGGCTGTGGAAGCTTCGACTGACCAGGTTGCCGCTTTGTTGGACTT
CTGTTCCTGAGGAATTGACG

Downstream 100 bases:

>100_bases
TCCTCGGTATGAACACGATCCGGTATGTGCGTTATAGTAGTGATGTAGCGCGCTGGACGTGCGCAAATCCCCACTGACGA
TCGTCTCGTGATCCCGGCGT

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDNSVRVRVPATSANLGPGYDCIGLALDLWDEVSVGVLDRPGVMIDVTGEGADTVPHDESHLVMATLRQGLVELGYPHPD
AGLHLTAINSIPQSRGLGSSAAAVVSGLALAWGLARPGFPLDRSALLTMAAAIEGHPDNAAPAILGGAQLAWLDGEAVNH
IGLTVNPSIVFRVYVPDRLVPTALARQVLPEQVDRVDAVHQVLAASLLVTALTTSPEHLLAATQDWIHQPYRRALMPESA
ALTDRLRGRGVATVISGAGPTVLALGSRDQLEKVSDVDTAGFVAHDLVLGEGVHFF

Sequences:

>Translated_296_residues
MDNSVRVRVPATSANLGPGYDCIGLALDLWDEVSVGVLDRPGVMIDVTGEGADTVPHDESHLVMATLRQGLVELGYPHPD
AGLHLTAINSIPQSRGLGSSAAAVVSGLALAWGLARPGFPLDRSALLTMAAAIEGHPDNAAPAILGGAQLAWLDGEAVNH
IGLTVNPSIVFRVYVPDRLVPTALARQVLPEQVDRVDAVHQVLAASLLVTALTTSPEHLLAATQDWIHQPYRRALMPESA
ALTDRLRGRGVATVISGAGPTVLALGSRDQLEKVSDVDTAGFVAHDLVLGEGVHFF
>Mature_296_residues
MDNSVRVRVPATSANLGPGYDCIGLALDLWDEVSVGVLDRPGVMIDVTGEGADTVPHDESHLVMATLRQGLVELGYPHPD
AGLHLTAINSIPQSRGLGSSAAAVVSGLALAWGLARPGFPLDRSALLTMAAAIEGHPDNAAPAILGGAQLAWLDGEAVNH
IGLTVNPSIVFRVYVPDRLVPTALARQVLPEQVDRVDAVHQVLAASLLVTALTTSPEHLLAATQDWIHQPYRRALMPESA
ALTDRLRGRGVATVISGAGPTVLALGSRDQLEKVSDVDTAGFVAHDLVLGEGVHFF

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=288, Percent_Identity=31.25, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321814, Length=332, Percent_Identity=31.3253012048193, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_PROAC (Q6A8B2)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055961.1
- ProteinModelPortal:   Q6A8B2
- SMR:   Q6A8B2
- GeneID:   2932522
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1256
- NMPDR:   fig|267747.1.peg.1238
- HOGENOM:   HBG646290
- OMA:   FDCLGAA
- ProtClustDB:   CLSK2763851
- BioCyc:   PACN267747:PPA1256-MONOMER
- BRENDA:   2.7.1.39
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 31007; Mature: 31007

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNSVRVRVPATSANLGPGYDCIGLALDLWDEVSVGVLDRPGVMIDVTGEGADTVPHDES
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCEEEEECCCCCCCCCCCCC
HLVMATLRQGLVELGYPHPDAGLHLTAINSIPQSRGLGSSAAAVVSGLALAWGLARPGFP
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCC
LDRSALLTMAAAIEGHPDNAAPAILGGAQLAWLDGEAVNHIGLTVNPSIVFRVYVPDRLV
CCHHHHHHHHHHHCCCCCCCCCEEECCCEEEEECCCCCCEEEEEECCEEEEEEECCCCHH
PTALARQVLPEQVDRVDAVHQVLAASLLVTALTTSPEHLLAATQDWIHQPYRRALMPESA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHH
ALTDRLRGRGVATVISGAGPTVLALGSRDQLEKVSDVDTAGFVAHDLVLGEGVHFF
HHHHHHCCCCEEEEECCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDNSVRVRVPATSANLGPGYDCIGLALDLWDEVSVGVLDRPGVMIDVTGEGADTVPHDES
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCEEEEECCCCCCCCCCCCC
HLVMATLRQGLVELGYPHPDAGLHLTAINSIPQSRGLGSSAAAVVSGLALAWGLARPGFP
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCC
LDRSALLTMAAAIEGHPDNAAPAILGGAQLAWLDGEAVNHIGLTVNPSIVFRVYVPDRLV
CCHHHHHHHHHHHCCCCCCCCCEEECCCEEEEECCCCCCEEEEEECCEEEEEEECCCCHH
PTALARQVLPEQVDRVDAVHQVLAASLLVTALTTSPEHLLAATQDWIHQPYRRALMPESA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHH
ALTDRLRGRGVATVISGAGPTVLALGSRDQLEKVSDVDTAGFVAHDLVLGEGVHFF
HHHHHHCCCCEEEEECCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA