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The map label for this gene is ybjI [H]

Identifier: 50842697

GI number: 50842697

Start: 1325051

End: 1325839

Strand: Direct

Name: ybjI [H]

Synonym: PPA1216

Alternate gene names: 50842697

Gene position: 1325051-1325839 (Clockwise)

Preceding gene: 50842690

Following gene: 50842699

Centisome position: 51.75

GC content: 58.17

Gene sequence:

>789_bases
ATGGATGGCACCCTCCTTGACGGCGACGGGAACATTCCCTCGCAGCTATGGCCCCTACTGGAGACGATGAGGGATCGTGC
CATCGCCTTCGTACCGGCCTCGGGCCGCCAATGCACCACACTATCTACGATGTTCGGCTCTCACTTGCAGGGCATGCCAA
TCATCGCGGAAAACGGAACCTACGTCGTCCGTGACGGTGTCGATATCTCGTCGTCGACCATCGATCCTAACCTCACCCGT
GAGGTCATTACAGGGGTTCGGCAACTTCGCAACGACGGCCATGACGTTGGCTTGGTCGTCGCCACCAAATCCATCGCTTA
CCTTGAGCGCGGAGACGACCCCTTCGTTTCCCATGTAGCGACTTACTACCACAGCCACAGAATCGTCAAGGATCTCAGCG
AACACACCGACGACGTCATCAAGCTCTCCATCTACGACTTCAGGGAAGCCTCAGAGGCCACCTACCCGACGATGCAGCGC
ACCGCCCCCGAGCACCAAGTAATCCTCGCCACCCCTAACTGGGTCGACATCATGAACCCAGGCGTCAACAAGGGCAGTGC
CGTCGAGGCTGTCCAGCGCGACCTTGGCGTCACTGCTGATCAAACAGCCATCTTTGGCGACTTTCTCAACGACCTCGAGA
TGATGCCCATGGCGAAGTGGTCCTTTGCCATGGCCAACGGCCATCCCAACATTGTCAAAGCATCGAATTACGTGGCACCA
CCGAACACCGAAAACGGCGTCATTACGGTCCTTGCCGCGTTGCTAGGTACCCGCGTCCCGATCACGTGA

Upstream 100 bases:

>100_bases
TCACGCAGCAAGACGATTTCGCTATCCTGACCAGGTGGACATGCCAAATCCAACCCCGTTCCCTGCCCCCGCAGACGACC
TTCGCCTGGTGGTCTCCGAC

Downstream 100 bases:

>100_bases
CGGCCACAGGTCAGACCAGGGATAGCTCTTTGGCCTCGCGCCAGAGTTTTTCTCGGGCATTAGGATGAGCGGCATTGTCG
ATGAGTTGTCGCGCCTGCTC

Product: HAD superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MDGTLLDGDGNIPSQLWPLLETMRDRAIAFVPASGRQCTTLSTMFGSHLQGMPIIAENGTYVVRDGVDISSSTIDPNLTR
EVITGVRQLRNDGHDVGLVVATKSIAYLERGDDPFVSHVATYYHSHRIVKDLSEHTDDVIKLSIYDFREASEATYPTMQR
TAPEHQVILATPNWVDIMNPGVNKGSAVEAVQRDLGVTADQTAIFGDFLNDLEMMPMAKWSFAMANGHPNIVKASNYVAP
PNTENGVITVLAALLGTRVPIT

Sequences:

>Translated_262_residues
MDGTLLDGDGNIPSQLWPLLETMRDRAIAFVPASGRQCTTLSTMFGSHLQGMPIIAENGTYVVRDGVDISSSTIDPNLTR
EVITGVRQLRNDGHDVGLVVATKSIAYLERGDDPFVSHVATYYHSHRIVKDLSEHTDDVIKLSIYDFREASEATYPTMQR
TAPEHQVILATPNWVDIMNPGVNKGSAVEAVQRDLGVTADQTAIFGDFLNDLEMMPMAKWSFAMANGHPNIVKASNYVAP
PNTENGVITVLAALLGTRVPIT
>Mature_262_residues
MDGTLLDGDGNIPSQLWPLLETMRDRAIAFVPASGRQCTTLSTMFGSHLQGMPIIAENGTYVVRDGVDISSSTIDPNLTR
EVITGVRQLRNDGHDVGLVVATKSIAYLERGDDPFVSHVATYYHSHRIVKDLSEHTDDVIKLSIYDFREASEATYPTMQR
TAPEHQVILATPNWVDIMNPGVNKGSAVEAVQRDLGVTADQTAIFGDFLNDLEMMPMAKWSFAMANGHPNIVKASNYVAP
PNTENGVITVLAALLGTRVPIT

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=268, Percent_Identity=28.3582089552239, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787043, Length=281, Percent_Identity=25.2669039145907, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 28580; Mature: 28580

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGTLLDGDGNIPSQLWPLLETMRDRAIAFVPASGRQCTTLSTMFGSHLQGMPIIAENGT
CCCCEECCCCCCHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCC
YVVRDGVDISSSTIDPNLTREVITGVRQLRNDGHDVGLVVATKSIAYLERGDDPFVSHVA
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHEEEHHCCCCHHHHHHH
TYYHSHRIVKDLSEHTDDVIKLSIYDFREASEATYPTMQRTAPEHQVILATPNWVDIMNP
HHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCC
GVNKGSAVEAVQRDLGVTADQTAIFGDFLNDLEMMPMAKWSFAMANGHPNIVKASNYVAP
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHEEEEECCCCCEEECCCEECC
PNTENGVITVLAALLGTRVPIT
CCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDGTLLDGDGNIPSQLWPLLETMRDRAIAFVPASGRQCTTLSTMFGSHLQGMPIIAENGT
CCCCEECCCCCCHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCC
YVVRDGVDISSSTIDPNLTREVITGVRQLRNDGHDVGLVVATKSIAYLERGDDPFVSHVA
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHEEEHHCCCCHHHHHHH
TYYHSHRIVKDLSEHTDDVIKLSIYDFREASEATYPTMQRTAPEHQVILATPNWVDIMNP
HHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCC
GVNKGSAVEAVQRDLGVTADQTAIFGDFLNDLEMMPMAKWSFAMANGHPNIVKASNYVAP
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHEEEEECCCCCEEECCCEECC
PNTENGVITVLAALLGTRVPIT
CCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]