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The map label for this gene is hisS

Identifier: 50842649

GI number: 50842649

Start: 1270932

End: 1272296

Strand: Direct

Name: hisS

Synonym: PPA1169

Alternate gene names: 50842649

Gene position: 1270932-1272296 (Clockwise)

Preceding gene: 50842648

Following gene: 50842653

Centisome position: 49.64

GC content: 59.49

Gene sequence:

>1365_bases
ATGGCCCGTCTCAAGCCCCTGTCCGGTTTTCCCGAGTTCCTGCCCTCAGGACAGATGGTAGAGAACCATGTGACCCGGAT
TCTTGAGGAGACCTTCGAGCTTCATGGCTTCGCACCGATTCGTACTCGCGCCGTCGAGCCGATGGAACAGCTCACTCGCA
AGGGAGAAATCGACAAAGAAGTCTACGTCGTCGACCGGCTGCACGCCGACCCGCACGACACGCGTTCCGGGAAGGATCGC
CTTGGCCTGCACTTCGACCTGACCGTCCCGTTTGCACGTTACGTACTGGAGAATGCTGGACATCTGTATTTCCCGTTCCG
CCGGTACCAGATTCAGAAAGTGTGGCGTGGCGAGCGTCCGCAGGAGGGTCGCTACCGCGAGTTCACCCAGGCCGATATTG
ACATTGTTGGCGACCAGACCCTTGCTGAGCACCACGACGTCGAAACCCCGCTAGTGATGCTGTCAGCCCTTGAACGACTG
CACACTGAGCTGGGATTTCCGACCGTTACGATGCACGTCAACAACCGCAAGCTCTCCGAAGGGTTCTATCGCGGGCTCGG
CATCGCCGATCCGATGGCGGTGCTACAGCGGGTCGACAAGTACGACAAGATTGGCCCGGACGCCGTCCGCAAACTGCTTG
TCGACGAGTTGAACCTTTCTGATGACGCCGCGGCTAAGTGCGTCGCTCTGGCCTCCATTCAGTCGACCGACGACTCCTTT
ATTGCCAAGGTACGCGAGTTGGGGGTGGCCAATGACATGCTCGAAGAGGGCCTGGACTCCCTCAACCGGGTAGTTGCTGC
CGTTAATAAGGCGGTTCCCGGACGCATGGTTGCCAACCTCAAAATCGCGCGCGGGCTCGACTACTACACCGGAACCGTCT
ACGAAACTGAGCTCACCGGTCACGAGTCGATGGGTTCAGTGTGCTCGGGAGGTCGTTATGAGTCCCTTGCCTCCGATGGT
AAACATGTCTACCCGGGTGTGGGCATCTCTCTGGGCCTCACCCGTTTGCTGGCGCCGATCCTTTCCCGCGGCGAGCTGTC
TTCCTCACGTTCGGTTCCTTCGGCGGTTCTCGTTGCCGTTAATACTGAGGAGGATCGCGCTACTTCGGAGGTCATCGCTT
CCGCATTGCGTTCCCGCGGCATCCCATGCGAGGTAGCTCCGAAGGCTGACAAGTTCGGGAAGCAGATCAAGCATGCTGAT
CGTCGCGGCATACCATTTGTGTGGTTCCCGGGCGTCAAACACGCCGACTACCGTGACGCCGACACCGTCAAGGACATTCG
GTCGGGCGATCAGGTTGAGGCTGATGCTGGGGCGTGGAACCCGCCTACCGAAGACCTTCATCCCGGTGTGATCGGTACCT
GGTGA

Upstream 100 bases:

>100_bases
GGCCTCACCGCATGCGAATGCACCCGATCGCCCTCAGACTGGCACAATAAAGGCTGCGGGGTGACCCGCTGTTGTACTCA
GCTTTTCGAAGGATTTCCTC

Downstream 100 bases:

>100_bases
CCTCAGGCCAGGATGGCTCCCAGTAAACCTGGGACCTTACGACCGCGTGAGGGAACTCAGCAGGAGCGCGCGCGATCTTG
AACACGCGCCGTGACGATGT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MARLKPLSGFPEFLPSGQMVENHVTRILEETFELHGFAPIRTRAVEPMEQLTRKGEIDKEVYVVDRLHADPHDTRSGKDR
LGLHFDLTVPFARYVLENAGHLYFPFRRYQIQKVWRGERPQEGRYREFTQADIDIVGDQTLAEHHDVETPLVMLSALERL
HTELGFPTVTMHVNNRKLSEGFYRGLGIADPMAVLQRVDKYDKIGPDAVRKLLVDELNLSDDAAAKCVALASIQSTDDSF
IAKVRELGVANDMLEEGLDSLNRVVAAVNKAVPGRMVANLKIARGLDYYTGTVYETELTGHESMGSVCSGGRYESLASDG
KHVYPGVGISLGLTRLLAPILSRGELSSSRSVPSAVLVAVNTEEDRATSEVIASALRSRGIPCEVAPKADKFGKQIKHAD
RRGIPFVWFPGVKHADYRDADTVKDIRSGDQVEADAGAWNPPTEDLHPGVIGTW

Sequences:

>Translated_454_residues
MARLKPLSGFPEFLPSGQMVENHVTRILEETFELHGFAPIRTRAVEPMEQLTRKGEIDKEVYVVDRLHADPHDTRSGKDR
LGLHFDLTVPFARYVLENAGHLYFPFRRYQIQKVWRGERPQEGRYREFTQADIDIVGDQTLAEHHDVETPLVMLSALERL
HTELGFPTVTMHVNNRKLSEGFYRGLGIADPMAVLQRVDKYDKIGPDAVRKLLVDELNLSDDAAAKCVALASIQSTDDSF
IAKVRELGVANDMLEEGLDSLNRVVAAVNKAVPGRMVANLKIARGLDYYTGTVYETELTGHESMGSVCSGGRYESLASDG
KHVYPGVGISLGLTRLLAPILSRGELSSSRSVPSAVLVAVNTEEDRATSEVIASALRSRGIPCEVAPKADKFGKQIKHAD
RRGIPFVWFPGVKHADYRDADTVKDIRSGDQVEADAGAWNPPTEDLHPGVIGTW
>Mature_453_residues
ARLKPLSGFPEFLPSGQMVENHVTRILEETFELHGFAPIRTRAVEPMEQLTRKGEIDKEVYVVDRLHADPHDTRSGKDRL
GLHFDLTVPFARYVLENAGHLYFPFRRYQIQKVWRGERPQEGRYREFTQADIDIVGDQTLAEHHDVETPLVMLSALERLH
TELGFPTVTMHVNNRKLSEGFYRGLGIADPMAVLQRVDKYDKIGPDAVRKLLVDELNLSDDAAAKCVALASIQSTDDSFI
AKVRELGVANDMLEEGLDSLNRVVAAVNKAVPGRMVANLKIARGLDYYTGTVYETELTGHESMGSVCSGGRYESLASDGK
HVYPGVGISLGLTRLLAPILSRGELSSSRSVPSAVLVAVNTEEDRATSEVIASALRSRGIPCEVAPKADKFGKQIKHADR
RGIPFVWFPGVKHADYRDADTVKDIRSGDQVEADAGAWNPPTEDLHPGVIGTW

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=440, Percent_Identity=30, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI15029520, Length=436, Percent_Identity=30.2752293577982, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI1788861, Length=440, Percent_Identity=26.8181818181818, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=467, Percent_Identity=30.1927194860814, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71993693, Length=467, Percent_Identity=30.1927194860814, Blast_Score=144, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6325290, Length=399, Percent_Identity=28.8220551378446, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24643061, Length=450, Percent_Identity=29.7777777777778, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24643059, Length=373, Percent_Identity=29.7587131367292, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_PROAC (Q6A8J7)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055876.1
- ProteinModelPortal:   Q6A8J7
- SMR:   Q6A8J7
- GeneID:   2932985
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1169
- NMPDR:   fig|267747.1.peg.1153
- HOGENOM:   HBG616575
- OMA:   LERISHT
- ProtClustDB:   PRK00037
- BioCyc:   PACN267747:PPA1169-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 50277; Mature: 50145

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLKPLSGFPEFLPSGQMVENHVTRILEETFELHGFAPIRTRAVEPMEQLTRKGEIDKE
CCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
VYVVDRLHADPHDTRSGKDRLGLHFDLTVPFARYVLENAGHLYFPFRRYQIQKVWRGERP
EEEEEHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCC
QEGRYREFTQADIDIVGDQTLAEHHDVETPLVMLSALERLHTELGFPTVTMHVNNRKLSE
CCCCHHHHHHCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
GFYRGLGIADPMAVLQRVDKYDKIGPDAVRKLLVDELNLSDDAAAKCVALASIQSTDDSF
HHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
IAKVRELGVANDMLEEGLDSLNRVVAAVNKAVPGRMVANLKIARGLDYYTGTVYETELTG
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHCCCEEEEECCC
HESMGSVCSGGRYESLASDGKHVYPGVGISLGLTRLLAPILSRGELSSSRSVPSAVLVAV
CHHHHHHHCCCCHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
NTEEDRATSEVIASALRSRGIPCEVAPKADKFGKQIKHADRRGIPFVWFPGVKHADYRDA
ECCHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCH
DTVKDIRSGDQVEADAGAWNPPTEDLHPGVIGTW
HHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARLKPLSGFPEFLPSGQMVENHVTRILEETFELHGFAPIRTRAVEPMEQLTRKGEIDKE
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
VYVVDRLHADPHDTRSGKDRLGLHFDLTVPFARYVLENAGHLYFPFRRYQIQKVWRGERP
EEEEEHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCC
QEGRYREFTQADIDIVGDQTLAEHHDVETPLVMLSALERLHTELGFPTVTMHVNNRKLSE
CCCCHHHHHHCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
GFYRGLGIADPMAVLQRVDKYDKIGPDAVRKLLVDELNLSDDAAAKCVALASIQSTDDSF
HHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
IAKVRELGVANDMLEEGLDSLNRVVAAVNKAVPGRMVANLKIARGLDYYTGTVYETELTG
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHCCCEEEEECCC
HESMGSVCSGGRYESLASDGKHVYPGVGISLGLTRLLAPILSRGELSSSRSVPSAVLVAV
CHHHHHHHCCCCHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
NTEEDRATSEVIASALRSRGIPCEVAPKADKFGKQIKHADRRGIPFVWFPGVKHADYRDA
ECCHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCH
DTVKDIRSGDQVEADAGAWNPPTEDLHPGVIGTW
HHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA