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The map label for this gene is hisA

Identifier: 50842636

GI number: 50842636

Start: 1257363

End: 1258130

Strand: Direct

Name: hisA

Synonym: PPA1156

Alternate gene names: 50842636

Gene position: 1257363-1258130 (Clockwise)

Preceding gene: 50842635

Following gene: 50842637

Centisome position: 49.11

GC content: 60.55

Gene sequence:

>768_bases
GTGACCGGAGAACTCACGCTGCTTCCAGCCGTTGATGTCCAGGGCGGTCGTGCCGTCCAGCTGCAACAGGGGGTGGCCAG
TTCTGAACGGGCATTTGGCGACCCTCTCGATGTCGCTCAGAAGTGGCAAGGCCTGGGAGCCCAATGGATTCACCTGGTTG
ATCTTGATGCTGCCTTCGGACATGGATCCAATACGAAGATCATCAGCAGCGTCGTTGAGCAGTTGGACATTAACGTTGAG
GTTTCGGGGGGTATCCGAGATCAAAGGAGTCTCGAGTCCGCATTGTCCGCTGGCGCGACCCGGGTCAATATCGGTACCGC
TGCCCTCGAGGACCCTCAGTGGTGTGACGAAGTAGTCGGGCGTTATGGCGAACAGGTAGCGATCGGCTTAGACGTGCGTG
GCGATCAGCTGGCGGCTCGTGGATGGACGCGCGAGGGCGGAAAGGTTCTCGACGTTCTGGCGCGCTTGGAGGACGCCGGG
TGTAGGCGATACGTCGTTACCGACGTCGCCAGTGATGGCATGCTAACCGGCCCTAATTACGAGCTTCTGGGGCGGATTTG
CGCTCGTACCCAAGGCAAAGTTGTCGCTTCAGGTGGTATTGCCGCGCTGGAGGATTTGCGACGCCTGCGCGGTTTAGTCC
CGATCGGTGTTGAGGGCGCGATCGTCGGGACGGCTCTGTACGTCGGCAATTTCACCTTGCCTGCGGCCTTGGATGTGTGC
CGGGCCCCCATCCCGGCTGATTCATCCCCGATGCCTCCTACCGACTGA

Upstream 100 bases:

>100_bases
CCCGGTAACGTCCACGCAGTTCCATCCTGAAAAGTCGGGGATAGCAGGTCTCGCTTTGCTCACACGCTGGCTGAATCAGC
TGAGTTGATAGCCTGATTCT

Downstream 100 bases:

>100_bases
GCGCCGATTTATCGTGGTTTTTCTTCCTCTGATTCGTTCTCAGACGGGGAGTGTAGACTGTTCCACTGTGTGCGTAGGCT
CGTCCTGCGCCGGATGCTTA

Product: phosphoribosyl isomerase A

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTGELTLLPAVDVQGGRAVQLQQGVASSERAFGDPLDVAQKWQGLGAQWIHLVDLDAAFGHGSNTKIISSVVEQLDINVE
VSGGIRDQRSLESALSAGATRVNIGTAALEDPQWCDEVVGRYGEQVAIGLDVRGDQLAARGWTREGGKVLDVLARLEDAG
CRRYVVTDVASDGMLTGPNYELLGRICARTQGKVVASGGIAALEDLRRLRGLVPIGVEGAIVGTALYVGNFTLPAALDVC
RAPIPADSSPMPPTD

Sequences:

>Translated_255_residues
MTGELTLLPAVDVQGGRAVQLQQGVASSERAFGDPLDVAQKWQGLGAQWIHLVDLDAAFGHGSNTKIISSVVEQLDINVE
VSGGIRDQRSLESALSAGATRVNIGTAALEDPQWCDEVVGRYGEQVAIGLDVRGDQLAARGWTREGGKVLDVLARLEDAG
CRRYVVTDVASDGMLTGPNYELLGRICARTQGKVVASGGIAALEDLRRLRGLVPIGVEGAIVGTALYVGNFTLPAALDVC
RAPIPADSSPMPPTD
>Mature_254_residues
TGELTLLPAVDVQGGRAVQLQQGVASSERAFGDPLDVAQKWQGLGAQWIHLVDLDAAFGHGSNTKIISSVVEQLDINVEV
SGGIRDQRSLESALSAGATRVNIGTAALEDPQWCDEVVGRYGEQVAIGLDVRGDQLAARGWTREGGKVLDVLARLEDAGC
RRYVVTDVASDGMLTGPNYELLGRICARTQGKVVASGGIAALEDLRRLRGLVPIGVEGAIVGTALYVGNFTLPAALDVCR
APIPADSSPMPPTD

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=241, Percent_Identity=34.4398340248963, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788336, Length=234, Percent_Identity=24.3589743589744, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_PROAC (Q6A8L1)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055863.1
- ProteinModelPortal:   Q6A8L1
- GeneID:   2931368
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1156
- NMPDR:   fig|267747.1.peg.1140
- HOGENOM:   HBG541613
- OMA:   CARYVVT
- ProtClustDB:   PRK14024
- BioCyc:   PACN267747:PPA1156-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR010188
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR01919

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26754; Mature: 26622

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGELTLLPAVDVQGGRAVQLQQGVASSERAFGDPLDVAQKWQGLGAQWIHLVDLDAAFG
CCCCEEEEEEEECCCCCEEEEHHCCCCCHHCCCCHHHHHHHHHCCCCEEEEEEEEHHHCC
HGSNTKIISSVVEQLDINVEVSGGIRDQRSLESALSAGATRVNIGTAALEDPQWCDEVVG
CCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHH
RYGEQVAIGLDVRGDQLAARGWTREGGKVLDVLARLEDAGCRRYVVTDVASDGMLTGPNY
HCCCEEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCH
ELLGRICARTQGKVVASGGIAALEDLRRLRGLVPIGVEGAIVGTALYVGNFTLPAALDVC
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHEECCCCHHHHHHHH
RAPIPADSSPMPPTD
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGELTLLPAVDVQGGRAVQLQQGVASSERAFGDPLDVAQKWQGLGAQWIHLVDLDAAFG
CCCEEEEEEEECCCCCEEEEHHCCCCCHHCCCCHHHHHHHHHCCCCEEEEEEEEHHHCC
HGSNTKIISSVVEQLDINVEVSGGIRDQRSLESALSAGATRVNIGTAALEDPQWCDEVVG
CCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHH
RYGEQVAIGLDVRGDQLAARGWTREGGKVLDVLARLEDAGCRRYVVTDVASDGMLTGPNY
HCCCEEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCH
ELLGRICARTQGKVVASGGIAALEDLRRLRGLVPIGVEGAIVGTALYVGNFTLPAALDVC
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHEECCCCHHHHHHHH
RAPIPADSSPMPPTD
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA