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The map label for this gene is hisH

Identifier: 50842635

GI number: 50842635

Start: 1256697

End: 1257350

Strand: Direct

Name: hisH

Synonym: PPA1155

Alternate gene names: 50842635

Gene position: 1256697-1257350 (Clockwise)

Preceding gene: 50842634

Following gene: 50842636

Centisome position: 49.08

GC content: 59.33

Gene sequence:

>654_bases
ATGCCGGTCGTGTCCCCAGCACTAAAGGCGCTCTCAATGTCTGATCCGGTGCGGGTCGGCATTATCGACCATGGATCAGG
AAACCTTCACTCCGCTAGACGTGCGTTGCGTCAGGTAGGTGCCGAGGTGATCGTTTCCAACGACCCGAGTGCCCTACTCG
ATACCGACGCTCTCGTCCTCCCCGGAGTGGGAGCGTTAGCGGTGTGTATGACAGGGTTGCGGGCTATGGGCGGGGCCCGT
CTTGTCCGTCAATGGGTTGACGAGGGACGACCTCTGCTGGGTATCTGCGTGGGCCACCAGATGCTTTTCGAGCGAGGACG
TGAACGCGACGTTGACGTCAAGTGCCTTGGTGTTCTTCCTGGCGTCGTTGAGGAGCTGCCAGCAGAGCGACTGCCCCATA
TGGGGTGGAACACAGTGACACCGGCCACCGACTCAGCCTTATTCCAGGGCGTGCAAGAGAGGTTCTACTTCGTCCATTCC
TATGGTGTGGTCGTTACCGGACCTCACGACCACTTCACCACTGCGACCCATCAGGGAGCCACCTTTGTCGCAGCCGCAGA
GTATGGCCCGGTAACGTCCACGCAGTTCCATCCTGAAAAGTCGGGGATAGCAGGTCTCGCTTTGCTCACACGCTGGCTGA
ATCAGCTGAGTTGA

Upstream 100 bases:

>100_bases
CTGCGTCTGCTGGCAGGACGCGACCCCCACCACATCTGCGAGGCCGAGTTCAAAGCGCTGGCCCGGGCGCTGAGAATTGC
GATAGCACCGGATCCCCGTA

Downstream 100 bases:

>100_bases
TAGCCTGATTCTGTGACCGGAGAACTCACGCTGCTTCCAGCCGTTGATGTCCAGGGCGGTCGTGCCGTCCAGCTGCAACA
GGGGGTGGCCAGTTCTGAAC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MPVVSPALKALSMSDPVRVGIIDHGSGNLHSARRALRQVGAEVIVSNDPSALLDTDALVLPGVGALAVCMTGLRAMGGAR
LVRQWVDEGRPLLGICVGHQMLFERGRERDVDVKCLGVLPGVVEELPAERLPHMGWNTVTPATDSALFQGVQERFYFVHS
YGVVVTGPHDHFTTATHQGATFVAAAEYGPVTSTQFHPEKSGIAGLALLTRWLNQLS

Sequences:

>Translated_217_residues
MPVVSPALKALSMSDPVRVGIIDHGSGNLHSARRALRQVGAEVIVSNDPSALLDTDALVLPGVGALAVCMTGLRAMGGAR
LVRQWVDEGRPLLGICVGHQMLFERGRERDVDVKCLGVLPGVVEELPAERLPHMGWNTVTPATDSALFQGVQERFYFVHS
YGVVVTGPHDHFTTATHQGATFVAAAEYGPVTSTQFHPEKSGIAGLALLTRWLNQLS
>Mature_216_residues
PVVSPALKALSMSDPVRVGIIDHGSGNLHSARRALRQVGAEVIVSNDPSALLDTDALVLPGVGALAVCMTGLRAMGGARL
VRQWVDEGRPLLGICVGHQMLFERGRERDVDVKCLGVLPGVVEELPAERLPHMGWNTVTPATDSALFQGVQERFYFVHSY
GVVVTGPHDHFTTATHQGATFVAAAEYGPVTSTQFHPEKSGIAGLALLTRWLNQLS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=205, Percent_Identity=38.5365853658537, Blast_Score=120, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=32.0754716981132, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23208; Mature: 23077

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVSPALKALSMSDPVRVGIIDHGSGNLHSARRALRQVGAEVIVSNDPSALLDTDALVL
CCCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCHHHCCCCHHH
PGVGALAVCMTGLRAMGGARLVRQWVDEGRPLLGICVGHQMLFERGRERDVDVKCLGVLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCEEEEHHHH
GVVEELPAERLPHMGWNTVTPATDSALFQGVQERFYFVHSYGVVVTGPHDHFTTATHQGA
HHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHEEHEECCEEEECCCCCCCCCCCCCC
TFVAAAEYGPVTSTQFHPEKSGIAGLALLTRWLNQLS
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVVSPALKALSMSDPVRVGIIDHGSGNLHSARRALRQVGAEVIVSNDPSALLDTDALVL
CCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCHHHCCCCHHH
PGVGALAVCMTGLRAMGGARLVRQWVDEGRPLLGICVGHQMLFERGRERDVDVKCLGVLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCEEEEHHHH
GVVEELPAERLPHMGWNTVTPATDSALFQGVQERFYFVHSYGVVVTGPHDHFTTATHQGA
HHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHEEHEECCEEEECCCCCCCCCCCCCC
TFVAAAEYGPVTSTQFHPEKSGIAGLALLTRWLNQLS
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA