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The map label for this gene is lacF [H]

Identifier: 50842628

GI number: 50842628

Start: 1248298

End: 1248618

Strand: Direct

Name: lacF [H]

Synonym: PPA1149

Alternate gene names: 50842628

Gene position: 1248298-1248618 (Clockwise)

Preceding gene: 50842627

Following gene: 50842629

Centisome position: 48.76

GC content: 52.34

Gene sequence:

>321_bases
ATGCCAGAGATCGAAACCGCCATTTTTACCATCATCACGAGCGCTGGTGAGGCGCGTGCGTCGCTGTATCAGGCTCTTGA
CAAAGCACGGGCTGGCGAGATGGCTGAGAGTGAACAGTGCATGATTAGGGCGGACACACAGTTGAAGAAAGCCCATGATA
TGCAGACCGAGCTCATCACGCGTGATCTCAACGGCTCGCTGCCGATGTCGCTTTTACTCGTCCATGCCCAAGACCAGTTG
ATGACGACGATGTCAGAACAGTCTCTCATTGAGCACATGATTGGACTAATTCGAGACATAGGTATCAACGGTGGGAAGTG
A

Upstream 100 bases:

>100_bases
TGAGGATGTATGACAGGGATCAACTCAGAAAAGAGCAATGCGAAGAAGGTGGAGCATGACTTCATATTGTGGGAGTGACA
GCCCGGATGGTGGGGCGGTG

Downstream 100 bases:

>100_bases
CTGCTCGATTATCGTAGGTTTGGAAGGTGCGGTATTCGGGTGTTTTCGCGTGGTGCCCGGTATGCTGCAGGGTGCAGAGT
GCTGTGAGACGGTCCCGTGA

Product: PTS cellobiose-specific component IIA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Lac; EIII-Lac; PTS system lactose-specific EIIA component [H]

Number of amino acids: Translated: 106; Mature: 105

Protein sequence:

>106_residues
MPEIETAIFTIITSAGEARASLYQALDKARAGEMAESEQCMIRADTQLKKAHDMQTELITRDLNGSLPMSLLLVHAQDQL
MTTMSEQSLIEHMIGLIRDIGINGGK

Sequences:

>Translated_106_residues
MPEIETAIFTIITSAGEARASLYQALDKARAGEMAESEQCMIRADTQLKKAHDMQTELITRDLNGSLPMSLLLVHAQDQL
MTTMSEQSLIEHMIGLIRDIGINGGK
>Mature_105_residues
PEIETAIFTIITSAGEARASLYQALDKARAGEMAESEQCMIRADTQLKKAHDMQTELITRDLNGSLPMSLLLVHAQDQLM
TTMSEQSLIEHMIGLIRDIGINGGK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788031, Length=95, Percent_Identity=40, Blast_Score=65, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 11660; Mature: 11528

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
7.5 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
6.7 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEIETAIFTIITSAGEARASLYQALDKARAGEMAESEQCMIRADTQLKKAHDMQTELIT
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHH
RDLNGSLPMSLLLVHAQDQLMTTMSEQSLIEHMIGLIRDIGINGGK
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PEIETAIFTIITSAGEARASLYQALDKARAGEMAESEQCMIRADTQLKKAHDMQTELIT
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHH
RDLNGSLPMSLLLVHAQDQLMTTMSEQSLIEHMIGLIRDIGINGGK
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2125052; 1901863; 9261069 [H]