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The map label for this gene is gatB

Identifier: 50842603

GI number: 50842603

Start: 1222594

End: 1224087

Strand: Direct

Name: gatB

Synonym: PPA1124

Alternate gene names: 50842603

Gene position: 1222594-1224087 (Clockwise)

Preceding gene: 50842602

Following gene: 50842605

Centisome position: 47.75

GC content: 59.91

Gene sequence:

>1494_bases
ATGACCGACGAGCTGTTGCCCTACGACGAAGTGATGGCCAATTATGAGCCGGTCGTCGGGCTGGAGGTACATGTCGAGCT
GTCCACCGCGACCAAGATGTTCTGCGGATGCTCAACCGATACTGACAAGGATCCCAATACCAATGTTTGCCCGGTTTGCC
TGGGTTTGCCCGGATCCATGCCGGCCATCAACGGTCATGCCGTCGAATCGGCCATTCGGATTGGTCTGGCTCTCAACTGT
CACATTGCCCAATGGTGCCGCATGGCCCGGAAGAACTACTTCTACCCGGACATGACGAAGAATTATCAGACCTCCCAATA
CGACGAACCAATTTGCTATGACGGCTGGCTCGACGTCGAGGCCGATGGCGAGACCTTTCGCGTCGAGATCGAGCGTGCTC
ACATGGAGGAAGACGCTGGAAAATCCCTCCATGTCGGAGGTTCCGACGGGCGTATTTCCGGTGCTAGTCACTCCCTCATG
GACTACAACCGGGCCGGTGTTCCCCTCATCGAGATCGTCACTAAACCGATCCGTGGGCTGGGAGCCAAAGCACCTCAAGT
TGCGCGTGCCTATATGGCTCAGTTACGGGACATCATGATCGCTCTTGGGGTTTCCGAGGCCAGAATGGAGCGCGGCAACC
TACGTTGCGACGCCAATGTCTCCCTCATGCCGCGTGGCAGCGAGACGTTAGGCACCCGTACCGAGACGAAGAACGTCAAC
TCGTTGCGCTCTGTGGAAGGAGCGCTGAGGTACGAAATTCAGCGTCAGGGATACCTGCTGTCTGAGGGACGCAAGGTCCG
TCAGCAGACCCGAATGTGGCAAGAGGCCGGCGAATACACCATCGCCGGACGCGACAAGTCTGACGCCGAGGACTACCGCT
ATTTCCCCGAGCCAGATCTGGTGCCGATTGCACCGTCGCGGGAGTGGGTTGAGCAGCTGCGTGCCGAGTTGCCAGAGCTG
CCAGCCGCCAAAAAGGCGCGTCTGTCGTCCCAATGGCAGTTCTCCGAAGCGGACATGACCTCGGCCGTCAACGCAGGTGC
CCTTGACCTCCTCGAGGCGACAGTCGATGCCGGCTGCCAGCCCGCTGCGGCCCGTAAATGGTGGCTGACTGAGCTGTCGC
GTCGCGCCAACGAGGCTGGTGTGGACTTGGCCGAGGTTGGTATGACGCCTTGTCAGGTCGCTCAGCTGCAAAAGCTCGTC
GATGACGGCACGTTGACCGACAAACTAGCTCGTCAGGTGATTGACGGAGTGATTGCTGGTGAGGGGGACCCCCAGCAGGT
TGTCGACGGTCGTGGCTTGGCTGTCGTCTCTGACGACGCGGCCTTGGGCTCCGCCGTTGACGATGTCATCGCCGCTAACC
CGCAGATCGCGGAGAAGATCCGTGGTGGGAAGGTGCAGGCCGCTGGTGCCCTCATCGGTCAGATTATGAAGGTCATGAAG
GGCCAGGCCGACGCCAAGCGGGTCAGGGAGCTCATCTTAGAGAAGCTGTCCTGA

Upstream 100 bases:

>100_bases
ACGATCGAATCTACCGAGTTGGGGCCGCTCTAGAACGATTACTACATGAGAAGTGGGGCGCCCCGCTGCTAGCGAAGGCT
CCCGAAGTGAGGGGAGAGCG

Downstream 100 bases:

>100_bases
CTCGTAATGCGCTCCGGCTAACTCACAGTGAGCGGTCGGAGCGCATGAACGCCGCGATCGTTGCGGGCTCGCCAAGCACA
TCCACCTGGGCCACCGATGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTDELLPYDEVMANYEPVVGLEVHVELSTATKMFCGCSTDTDKDPNTNVCPVCLGLPGSMPAINGHAVESAIRIGLALNC
HIAQWCRMARKNYFYPDMTKNYQTSQYDEPICYDGWLDVEADGETFRVEIERAHMEEDAGKSLHVGGSDGRISGASHSLM
DYNRAGVPLIEIVTKPIRGLGAKAPQVARAYMAQLRDIMIALGVSEARMERGNLRCDANVSLMPRGSETLGTRTETKNVN
SLRSVEGALRYEIQRQGYLLSEGRKVRQQTRMWQEAGEYTIAGRDKSDAEDYRYFPEPDLVPIAPSREWVEQLRAELPEL
PAAKKARLSSQWQFSEADMTSAVNAGALDLLEATVDAGCQPAAARKWWLTELSRRANEAGVDLAEVGMTPCQVAQLQKLV
DDGTLTDKLARQVIDGVIAGEGDPQQVVDGRGLAVVSDDAALGSAVDDVIAANPQIAEKIRGGKVQAAGALIGQIMKVMK
GQADAKRVRELILEKLS

Sequences:

>Translated_497_residues
MTDELLPYDEVMANYEPVVGLEVHVELSTATKMFCGCSTDTDKDPNTNVCPVCLGLPGSMPAINGHAVESAIRIGLALNC
HIAQWCRMARKNYFYPDMTKNYQTSQYDEPICYDGWLDVEADGETFRVEIERAHMEEDAGKSLHVGGSDGRISGASHSLM
DYNRAGVPLIEIVTKPIRGLGAKAPQVARAYMAQLRDIMIALGVSEARMERGNLRCDANVSLMPRGSETLGTRTETKNVN
SLRSVEGALRYEIQRQGYLLSEGRKVRQQTRMWQEAGEYTIAGRDKSDAEDYRYFPEPDLVPIAPSREWVEQLRAELPEL
PAAKKARLSSQWQFSEADMTSAVNAGALDLLEATVDAGCQPAAARKWWLTELSRRANEAGVDLAEVGMTPCQVAQLQKLV
DDGTLTDKLARQVIDGVIAGEGDPQQVVDGRGLAVVSDDAALGSAVDDVIAANPQIAEKIRGGKVQAAGALIGQIMKVMK
GQADAKRVRELILEKLS
>Mature_496_residues
TDELLPYDEVMANYEPVVGLEVHVELSTATKMFCGCSTDTDKDPNTNVCPVCLGLPGSMPAINGHAVESAIRIGLALNCH
IAQWCRMARKNYFYPDMTKNYQTSQYDEPICYDGWLDVEADGETFRVEIERAHMEEDAGKSLHVGGSDGRISGASHSLMD
YNRAGVPLIEIVTKPIRGLGAKAPQVARAYMAQLRDIMIALGVSEARMERGNLRCDANVSLMPRGSETLGTRTETKNVNS
LRSVEGALRYEIQRQGYLLSEGRKVRQQTRMWQEAGEYTIAGRDKSDAEDYRYFPEPDLVPIAPSREWVEQLRAELPELP
AAKKARLSSQWQFSEADMTSAVNAGALDLLEATVDAGCQPAAARKWWLTELSRRANEAGVDLAEVGMTPCQVAQLQKLVD
DGTLTDKLARQVIDGVIAGEGDPQQVVDGRGLAVVSDDAALGSAVDDVIAANPQIAEKIRGGKVQAAGALIGQIMKVMKG
QADAKRVRELILEKLS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=503, Percent_Identity=33.5984095427435, Blast_Score=257, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI115534094, Length=310, Percent_Identity=36.1290322580645, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319391, Length=524, Percent_Identity=25.763358778626, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45550808, Length=504, Percent_Identity=29.3650793650794, Blast_Score=204, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_PROAC (Q6A8P4)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055830.1
- ProteinModelPortal:   Q6A8P4
- SMR:   Q6A8P4
- GeneID:   2932923
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1124
- NMPDR:   fig|267747.1.peg.1107
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   PACN267747:PPA1124-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54286; Mature: 54155

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDELLPYDEVMANYEPVVGLEVHVELSTATKMFCGCSTDTDKDPNTNVCPVCLGLPGSM
CCCCCCCHHHHHCCCCCEEEEEEEEEEHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC
PAINGHAVESAIRIGLALNCHIAQWCRMARKNYFYPDMTKNYQTSQYDEPICYDGWLDVE
CCCCCHHHHHHHHHHEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCEEEEE
ADGETFRVEIERAHMEEDAGKSLHVGGSDGRISGASHSLMDYNRAGVPLIEIVTKPIRGL
CCCCEEEEEEEHHHHHHHCCCEEEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHC
GAKAPQVARAYMAQLRDIMIALGVSEARMERGNLRCDANVSLMPRGSETLGTRTETKNVN
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCCEECCCCCHHCCCCCCCCCHH
SLRSVEGALRYEIQRQGYLLSEGRKVRQQTRMWQEAGEYTIAGRDKSDAEDYRYFPEPDL
HHHHHHHHHHHHHHHCCEEHHCCHHHHHHHHHHHHCCCEEEECCCCCCHHHCCCCCCCCC
VPIAPSREWVEQLRAELPELPAAKKARLSSQWQFSEADMTSAVNAGALDLLEATVDAGCQ
EECCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCC
PAAARKWWLTELSRRANEAGVDLAEVGMTPCQVAQLQKLVDDGTLTDKLARQVIDGVIAG
CHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
EGDPQQVVDGRGLAVVSDDAALGSAVDDVIAANPQIAEKIRGGKVQAAGALIGQIMKVMK
CCCHHHHHCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHC
GQADAKRVRELILEKLS
CCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDELLPYDEVMANYEPVVGLEVHVELSTATKMFCGCSTDTDKDPNTNVCPVCLGLPGSM
CCCCCCHHHHHCCCCCEEEEEEEEEEHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC
PAINGHAVESAIRIGLALNCHIAQWCRMARKNYFYPDMTKNYQTSQYDEPICYDGWLDVE
CCCCCHHHHHHHHHHEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCEEEEE
ADGETFRVEIERAHMEEDAGKSLHVGGSDGRISGASHSLMDYNRAGVPLIEIVTKPIRGL
CCCCEEEEEEEHHHHHHHCCCEEEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHC
GAKAPQVARAYMAQLRDIMIALGVSEARMERGNLRCDANVSLMPRGSETLGTRTETKNVN
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCCEECCCCCHHCCCCCCCCCHH
SLRSVEGALRYEIQRQGYLLSEGRKVRQQTRMWQEAGEYTIAGRDKSDAEDYRYFPEPDL
HHHHHHHHHHHHHHHCCEEHHCCHHHHHHHHHHHHCCCEEEECCCCCCHHHCCCCCCCCC
VPIAPSREWVEQLRAELPELPAAKKARLSSQWQFSEADMTSAVNAGALDLLEATVDAGCQ
EECCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCC
PAAARKWWLTELSRRANEAGVDLAEVGMTPCQVAQLQKLVDDGTLTDKLARQVIDGVIAG
CHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
EGDPQQVVDGRGLAVVSDDAALGSAVDDVIAANPQIAEKIRGGKVQAAGALIGQIMKVMK
CCCHHHHHCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHC
GQADAKRVRELILEKLS
CCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA