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The map label for this gene is gatA

Identifier: 50842602

GI number: 50842602

Start: 1221092

End: 1222597

Strand: Direct

Name: gatA

Synonym: PPA1123

Alternate gene names: 50842602

Gene position: 1221092-1222597 (Clockwise)

Preceding gene: 50842601

Following gene: 50842603

Centisome position: 47.69

GC content: 61.16

Gene sequence:

>1506_bases
GTGAACGAGCTTCTGACGTTGACCGCCGCCGAGTTAGGCGAGCGGATTGCGGCCCGACAGATCTCTAGCGAAGAGGTCAC
CCAGGCGCATTTGGATCGCATTAGCGAGGTTGACGGCGACATCCATGCCTTCCTGCTCGTTGACCACGCTGGGGCTCTCG
ACGCTGCTCGTCGCATTGATGCCCGCATTGCAGATGGCGAGCATCTCGGTCCGCTGGCGGGGGTGCCGCTGGCTGTTAAG
GATCTTTTCTGCACGAAAGGGATCGCCACTACAGCGTCCTCCCAGATGCTCGAAGGGTGGATCCCCCCGTACGACTCCAC
TATCGTCACCCGCTGTAAGGATGCCGGAATGGTGATCCTCGGCAAGACGAACCTTGATGAATTCGCTATGGGGTCGTCAA
CGGAAACTTCGGCCTTCGGGCCTACCCATAATCCCTGGGACCTTGAACGGGTACCGGGTGGTTCCGGTGGTGGTTCGGCG
GCTAGCTTGGCTTCCTTCCAGGCCCCGTTGGCTTTGGGCACTGATACCGGTGGCTCGATCCGCCAGCCTGGAGCGGTGAC
CGGCACCGTCGGGATCAAGCCGACCTACGGTTCGACCTCCCGATACGGCGTCATCGCTATGGCTTCATCTTTGGATACTC
CTGGGCCCTGCGCCCGTACCGTCCTTGACGCCGCGTTGCTCCATCAGGCCATTGCCGGTCACGACGCTATGGACCAGACC
ACGATTAATCAGCCCACCCCGGCGGTCGTTGAGGCTGCGCGGCAAACAGACGTTTCCGGCGTGCGCATTGGCGTCGTCAC
GGAGTTGAGCGGGCAGGTTTACGACCCCCAGGTCGAGGCCCGGTTCCACGAGGCTGTCGAGATGCTAATAGAGGCGGGGG
CTGAGGTCGTTGAGGTCTCTTGCCCGAACTTTGACCTCGCCTTACCTGCTTATTACCTTATTCAGCCTGCCGAGGTGTCT
AGCAACCTGGCTCGTTACGACGCCATGCGTTACGGCTTACGCGTCAATGACGACGGCGAGCATTCTGCCGAGCAGGTGAT
GCGAGCCACCCGCGGTGCTGGACTTGGGGCCGAGGCCAAGCGTCGCATCATCTTGGGTACCTATGCCTTGTCGGCTGGGT
ACTATGACGCCTACTACGGCTCGGCTCAGAAAGTCCGTACCCTCATCCAACGCGACTTCGAGAAAGCATGGCAGATGTGC
GATGTGCTCGTGTCACCGGCCACGCCAACGACTGCCTTCCGGCTGGGTGAGCGTACTGCTGACCCGATGGCGATGTACCG
CTCCGATCTATGCACGGTCCCGGCCAATATGGCCGGAAGTCCCGCAGGATCTTTCCCGATCGGTCTATCAGAGACCGACG
GCATGCCCGTCGGCATGCAGGTGATGGCGCCAATCATGGCGGACGATCGAATCTACCGAGTTGGGGCCGCTCTAGAACGA
TTACTACATGAGAAGTGGGGCGCCCCGCTGCTAGCGAAGGCTCCCGAAGTGAGGGGAGAGCGATGA

Upstream 100 bases:

>100_bases
TCATCAAGGAGTCAATGTCCTCCCATGACGCTCTGGCCATGGCCCCGCGCAGTGAGAACCAACGTTTTCGCGTGCCTCGC
ATCCTGGACGAGGAGGCACA

Downstream 100 bases:

>100_bases
CCGACGAGCTGTTGCCCTACGACGAAGTGATGGCCAATTATGAGCCGGTCGTCGGGCTGGAGGTACATGTCGAGCTGTCC
ACCGCGACCAAGATGTTCTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MNELLTLTAAELGERIAARQISSEEVTQAHLDRISEVDGDIHAFLLVDHAGALDAARRIDARIADGEHLGPLAGVPLAVK
DLFCTKGIATTASSQMLEGWIPPYDSTIVTRCKDAGMVILGKTNLDEFAMGSSTETSAFGPTHNPWDLERVPGGSGGGSA
ASLASFQAPLALGTDTGGSIRQPGAVTGTVGIKPTYGSTSRYGVIAMASSLDTPGPCARTVLDAALLHQAIAGHDAMDQT
TINQPTPAVVEAARQTDVSGVRIGVVTELSGQVYDPQVEARFHEAVEMLIEAGAEVVEVSCPNFDLALPAYYLIQPAEVS
SNLARYDAMRYGLRVNDDGEHSAEQVMRATRGAGLGAEAKRRIILGTYALSAGYYDAYYGSAQKVRTLIQRDFEKAWQMC
DVLVSPATPTTAFRLGERTADPMAMYRSDLCTVPANMAGSPAGSFPIGLSETDGMPVGMQVMAPIMADDRIYRVGAALER
LLHEKWGAPLLAKAPEVRGER

Sequences:

>Translated_501_residues
MNELLTLTAAELGERIAARQISSEEVTQAHLDRISEVDGDIHAFLLVDHAGALDAARRIDARIADGEHLGPLAGVPLAVK
DLFCTKGIATTASSQMLEGWIPPYDSTIVTRCKDAGMVILGKTNLDEFAMGSSTETSAFGPTHNPWDLERVPGGSGGGSA
ASLASFQAPLALGTDTGGSIRQPGAVTGTVGIKPTYGSTSRYGVIAMASSLDTPGPCARTVLDAALLHQAIAGHDAMDQT
TINQPTPAVVEAARQTDVSGVRIGVVTELSGQVYDPQVEARFHEAVEMLIEAGAEVVEVSCPNFDLALPAYYLIQPAEVS
SNLARYDAMRYGLRVNDDGEHSAEQVMRATRGAGLGAEAKRRIILGTYALSAGYYDAYYGSAQKVRTLIQRDFEKAWQMC
DVLVSPATPTTAFRLGERTADPMAMYRSDLCTVPANMAGSPAGSFPIGLSETDGMPVGMQVMAPIMADDRIYRVGAALER
LLHEKWGAPLLAKAPEVRGER
>Mature_501_residues
MNELLTLTAAELGERIAARQISSEEVTQAHLDRISEVDGDIHAFLLVDHAGALDAARRIDARIADGEHLGPLAGVPLAVK
DLFCTKGIATTASSQMLEGWIPPYDSTIVTRCKDAGMVILGKTNLDEFAMGSSTETSAFGPTHNPWDLERVPGGSGGGSA
ASLASFQAPLALGTDTGGSIRQPGAVTGTVGIKPTYGSTSRYGVIAMASSLDTPGPCARTVLDAALLHQAIAGHDAMDQT
TINQPTPAVVEAARQTDVSGVRIGVVTELSGQVYDPQVEARFHEAVEMLIEAGAEVVEVSCPNFDLALPAYYLIQPAEVS
SNLARYDAMRYGLRVNDDGEHSAEQVMRATRGAGLGAEAKRRIILGTYALSAGYYDAYYGSAQKVRTLIQRDFEKAWQMC
DVLVSPATPTTAFRLGERTADPMAMYRSDLCTVPANMAGSPAGSFPIGLSETDGMPVGMQVMAPIMADDRIYRVGAALER
LLHEKWGAPLLAKAPEVRGER

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=484, Percent_Identity=37.396694214876, Blast_Score=292, Evalue=5e-79,
Organism=Homo sapiens, GI195972892, Length=493, Percent_Identity=23.3265720081136, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI166795287, Length=193, Percent_Identity=32.6424870466321, Blast_Score=66, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17543272, Length=437, Percent_Identity=36.3844393592677, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17537465, Length=519, Percent_Identity=24.8554913294798, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=30.7291666666667, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=259, Percent_Identity=27.027027027027, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=126, Percent_Identity=36.5079365079365, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=198, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=198, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=411, Percent_Identity=34.3065693430657, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319685, Length=410, Percent_Identity=31.219512195122, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=527, Percent_Identity=22.5806451612903, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648113, Length=443, Percent_Identity=39.7291196388262, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI45550774, Length=274, Percent_Identity=33.2116788321168, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648435, Length=274, Percent_Identity=33.2116788321168, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648437, Length=274, Percent_Identity=33.2116788321168, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=26.6019417475728, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI21356731, Length=237, Percent_Identity=30.8016877637131, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24644968, Length=518, Percent_Identity=25.6756756756757, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24652985, Length=507, Percent_Identity=23.8658777120316, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922090, Length=507, Percent_Identity=23.8658777120316, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652981, Length=507, Percent_Identity=23.8658777120316, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652983, Length=507, Percent_Identity=23.8658777120316, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648441, Length=202, Percent_Identity=32.6732673267327, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648439, Length=202, Percent_Identity=32.6732673267327, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_PROAC (Q6A8P5)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055829.1
- ProteinModelPortal:   Q6A8P5
- SMR:   Q6A8P5
- GeneID:   2932043
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1123
- NMPDR:   fig|267747.1.peg.1106
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   PACN267747:PPA1123-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53110; Mature: 53110

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 80-80 ACT_SITE 155-155 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELLTLTAAELGERIAARQISSEEVTQAHLDRISEVDGDIHAFLLVDHAGALDAARRID
CCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHH
ARIADGEHLGPLAGVPLAVKDLFCTKGIATTASSQMLEGWIPPYDSTIVTRCKDAGMVIL
HEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEE
GKTNLDEFAMGSSTETSAFGPTHNPWDLERVPGGSGGGSAASLASFQAPLALGTDTGGSI
ECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCC
RQPGAVTGTVGIKPTYGSTSRYGVIAMASSLDTPGPCARTVLDAALLHQAIAGHDAMDQT
CCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
TINQPTPAVVEAARQTDVSGVRIGVVTELSGQVYDPQVEARFHEAVEMLIEAGAEVVEVS
CCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCEEEEE
CPNFDLALPAYYLIQPAEVSSNLARYDAMRYGLRVNDDGEHSAEQVMRATRGAGLGAEAK
CCCCCCCCCHHHEEECHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCHHHC
RRIILGTYALSAGYYDAYYGSAQKVRTLIQRDFEKAWQMCDVLVSPATPTTAFRLGERTA
CEEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
DPMAMYRSDLCTVPANMAGSPAGSFPIGLSETDGMPVGMQVMAPIMADDRIYRVGAALER
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
LLHEKWGAPLLAKAPEVRGER
HHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNELLTLTAAELGERIAARQISSEEVTQAHLDRISEVDGDIHAFLLVDHAGALDAARRID
CCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHH
ARIADGEHLGPLAGVPLAVKDLFCTKGIATTASSQMLEGWIPPYDSTIVTRCKDAGMVIL
HEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEE
GKTNLDEFAMGSSTETSAFGPTHNPWDLERVPGGSGGGSAASLASFQAPLALGTDTGGSI
ECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCC
RQPGAVTGTVGIKPTYGSTSRYGVIAMASSLDTPGPCARTVLDAALLHQAIAGHDAMDQT
CCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
TINQPTPAVVEAARQTDVSGVRIGVVTELSGQVYDPQVEARFHEAVEMLIEAGAEVVEVS
CCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCEEEEE
CPNFDLALPAYYLIQPAEVSSNLARYDAMRYGLRVNDDGEHSAEQVMRATRGAGLGAEAK
CCCCCCCCCHHHEEECHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCHHHC
RRIILGTYALSAGYYDAYYGSAQKVRTLIQRDFEKAWQMCDVLVSPATPTTAFRLGERTA
CEEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
DPMAMYRSDLCTVPANMAGSPAGSFPIGLSETDGMPVGMQVMAPIMADDRIYRVGAALER
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
LLHEKWGAPLLAKAPEVRGER
HHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA