Click here to switch to the map view.

The map label for this gene is pfp [H]

Identifier: 50842568

GI number: 50842568

Start: 1182988

End: 1184187

Strand: Reverse

Name: pfp [H]

Synonym: PPA1090

Alternate gene names: 50842568

Gene position: 1184187-1182988 (Counterclockwise)

Preceding gene: 50842572

Following gene: 50842549

Centisome position: 46.25

GC content: 60.17

Gene sequence:

>1200_bases
ATGGCCGTCAAGAAGGTCGCACTCCTCACCGCGGGCGGATTTGCGCCGTGCCTATCCACCGCGATCAGTGGATTGATCCA
GCGCTACACCGAGGTAGCACCTGAGGTCGAGATCATCGCCTACAAGCACGGCTACGAGGGCCTGCTCAAGGGAGACTTCC
TCGAGGTCACCGACACCGTTCGCAAGAATGCCGAGATCCTCAAGCGTTTTGGTGGATCCCCGATTGGCAACTCGCGGGTC
AAGCTGACCAACGCCGCCGACCTCGTAAAGCGTGGTCTGGTCGCCGAAGGCGACGACCCGCTCAAGGTTGCCGCCGATCG
TCTGGTTGCCGACGGGGTTGACATTTTGCACACCATCGGTGGTGACGACACTAATACCACCGCCGCCGATCTGGCCGCCT
ATCTGGCTGAGAATAACTACGGCCTCACCGTCGTTGGCCTGCCGAAGACCATCGATAACGACGTCGTGCCGATTCGTCAA
TCGCTAGGTGCTTGGACGGCTGCGGAACAAGGTTCCCGCTTTGCTCAGAACATCGTTGGCGAGCACAACTCCGGATCACG
CATGCTCATCGTCCACGAAGTCATGGGCCGTAACTGCGGCTGGCTCACCGCCGCTACCGCCGCCAAATACCGCGAATGGC
TCGATACTCAACAGTGGCTACCTGAGATCGGGCTGTCCAAGAAGGCCTGGGATGTCCACGCCGTATATGTCCCCGAGGCT
CACATCGACCTTGAGGCCGAAGCCGCACGCCTCAACAAGGTCATGGACGAAGTTGGCAACGTCACTATCTTCCTGTCCGA
GGGGGCTGGCCTGGACGCCATTATTGAGGAGATGGAAAAAGACGGTCAGGAAGTCCCGCGCGATCCCTTTGGTCACGTCA
AGCTCGACAAGGTGAACCTGGGTGCTTGGTTCGGCAAACAGTTCGCCGACAAGCTCGGTGCCGAAAAGGTCATGGTCCAG
AAGTCCGGCTACTTCTCCCGTTCGGCAGCCTCGAACGAAGCTGACCTGGAGCTCATCGGCCGCTGCACTGACCTCGCTGT
CGATTGCGCCCTGGCCGGTAAGACTGGCGTTATCGGCCAGGACGAGGAGAACGGCGACACGTTGACGAACATCGCCTTCG
ATCGGATCAAGGGCGGCAAGCCTTTCGACACCACTCAGCCGTGGTTCACCGCCATGCTCAGCGAGATCGGGCAGGCCTGA

Upstream 100 bases:

>100_bases
TCGGTCCGGCAAGGGTTGACTTTTACTCCCCTGTGTGCCGGTTCGACACATACCATAGGCTTGCTAGCGTTACCCCACAC
GACAAACGAAAGGTTTCCCT

Downstream 100 bases:

>100_bases
CAGCTATGGCCCAACAGTGGCCCTTCCGAATGAACGTGGTCCCCGTCGGGACTGGTTGACAACGGAAGGGCCACCTTGCG
CTAAGCCCAAGTTGCCATTC

Product: pyrophosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAVKKVALLTAGGFAPCLSTAISGLIQRYTEVAPEVEIIAYKHGYEGLLKGDFLEVTDTVRKNAEILKRFGGSPIGNSRV
KLTNAADLVKRGLVAEGDDPLKVAADRLVADGVDILHTIGGDDTNTTAADLAAYLAENNYGLTVVGLPKTIDNDVVPIRQ
SLGAWTAAEQGSRFAQNIVGEHNSGSRMLIVHEVMGRNCGWLTAATAAKYREWLDTQQWLPEIGLSKKAWDVHAVYVPEA
HIDLEAEAARLNKVMDEVGNVTIFLSEGAGLDAIIEEMEKDGQEVPRDPFGHVKLDKVNLGAWFGKQFADKLGAEKVMVQ
KSGYFSRSAASNEADLELIGRCTDLAVDCALAGKTGVIGQDEENGDTLTNIAFDRIKGGKPFDTTQPWFTAMLSEIGQA

Sequences:

>Translated_399_residues
MAVKKVALLTAGGFAPCLSTAISGLIQRYTEVAPEVEIIAYKHGYEGLLKGDFLEVTDTVRKNAEILKRFGGSPIGNSRV
KLTNAADLVKRGLVAEGDDPLKVAADRLVADGVDILHTIGGDDTNTTAADLAAYLAENNYGLTVVGLPKTIDNDVVPIRQ
SLGAWTAAEQGSRFAQNIVGEHNSGSRMLIVHEVMGRNCGWLTAATAAKYREWLDTQQWLPEIGLSKKAWDVHAVYVPEA
HIDLEAEAARLNKVMDEVGNVTIFLSEGAGLDAIIEEMEKDGQEVPRDPFGHVKLDKVNLGAWFGKQFADKLGAEKVMVQ
KSGYFSRSAASNEADLELIGRCTDLAVDCALAGKTGVIGQDEENGDTLTNIAFDRIKGGKPFDTTQPWFTAMLSEIGQA
>Mature_398_residues
AVKKVALLTAGGFAPCLSTAISGLIQRYTEVAPEVEIIAYKHGYEGLLKGDFLEVTDTVRKNAEILKRFGGSPIGNSRVK
LTNAADLVKRGLVAEGDDPLKVAADRLVADGVDILHTIGGDDTNTTAADLAAYLAENNYGLTVVGLPKTIDNDVVPIRQS
LGAWTAAEQGSRFAQNIVGEHNSGSRMLIVHEVMGRNCGWLTAATAAKYREWLDTQQWLPEIGLSKKAWDVHAVYVPEAH
IDLEAEAARLNKVMDEVGNVTIFLSEGAGLDAIIEEMEKDGQEVPRDPFGHVKLDKVNLGAWFGKQFADKLGAEKVMVQK
SGYFSRSAASNEADLELIGRCTDLAVDCALAGKTGVIGQDEENGDTLTNIAFDRIKGGKPFDTTQPWFTAMLSEIGQA

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family. PFP subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=206, Percent_Identity=29.126213592233, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321679, Length=299, Percent_Identity=28.4280936454849, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323861, Length=249, Percent_Identity=26.9076305220884, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022953
- InterPro:   IPR000023
- InterPro:   IPR011405 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 42945; Mature: 42814

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKKVALLTAGGFAPCLSTAISGLIQRYTEVAPEVEIIAYKHGYEGLLKGDFLEVTDTV
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHH
RKNAEILKRFGGSPIGNSRVKLTNAADLVKRGLVAEGDDPLKVAADRLVADGVDILHTIG
HHHHHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHCC
GDDTNTTAADLAAYLAENNYGLTVVGLPKTIDNDVVPIRQSLGAWTAAEQGSRFAQNIVG
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCC
EHNSGSRMLIVHEVMGRNCGWLTAATAAKYREWLDTQQWLPEIGLSKKAWDVHAVYVPEA
CCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCC
HIDLEAEAARLNKVMDEVGNVTIFLSEGAGLDAIIEEMEKDGQEVPRDPFGHVKLDKVNL
CCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
GAWFGKQFADKLGAEKVMVQKSGYFSRSAASNEADLELIGRCTDLAVDCALAGKTGVIGQ
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCC
DEENGDTLTNIAFDRIKGGKPFDTTQPWFTAMLSEIGQA
CCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AVKKVALLTAGGFAPCLSTAISGLIQRYTEVAPEVEIIAYKHGYEGLLKGDFLEVTDTV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHH
RKNAEILKRFGGSPIGNSRVKLTNAADLVKRGLVAEGDDPLKVAADRLVADGVDILHTIG
HHHHHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHCC
GDDTNTTAADLAAYLAENNYGLTVVGLPKTIDNDVVPIRQSLGAWTAAEQGSRFAQNIVG
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCC
EHNSGSRMLIVHEVMGRNCGWLTAATAAKYREWLDTQQWLPEIGLSKKAWDVHAVYVPEA
CCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCC
HIDLEAEAARLNKVMDEVGNVTIFLSEGAGLDAIIEEMEKDGQEVPRDPFGHVKLDKVNL
CCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
GAWFGKQFADKLGAEKVMVQKSGYFSRSAASNEADLELIGRCTDLAVDCALAGKTGVIGQ
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCC
DEENGDTLTNIAFDRIKGGKPFDTTQPWFTAMLSEIGQA
CCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653240; 1317210 [H]