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The map label for this gene is tnaA

Identifier: 50842550

GI number: 50842550

Start: 1163084

End: 1164460

Strand: Direct

Name: tnaA

Synonym: PPA1068

Alternate gene names: 50842550

Gene position: 1163084-1164460 (Clockwise)

Preceding gene: 50842544

Following gene: 50842551

Centisome position: 45.43

GC content: 58.9

Gene sequence:

>1377_bases
ATGAAGTACATCCCCGAACCGTTTAAGATCAAGATGGTCGAACCGATTGCGATGACCTCCCGGGAGGAGCGCGCCAACAT
CCTCGCACGGGCACATTACAACATGTTCGGCCTACCCGCCGAGTCCGTCTACATCGACCTGCTCACCGATTCCGGTACCG
GCGCGATGAGTGATGCCCAGTGGGCGGCCGTGATGCGCGGTGACGAGGCGTACTCCGGTGGGCGCAGTTATATGCACCTC
ATGGACGTTGCCGGCAGTATCTTTGGTTACAGCTACATTCAGCCGGTGCATCAAGGCCGTGCTGCGGAAAAAGTGCTCAT
GCCGCTATTGGTCTCTCGTCCCGGCCAGCTCGTGGTGGCGAACACCTTCTTTGACACCACACGTGCCCACGTCGGCCTTG
CCGGTGGGCGTCCGGTCGACAATGTCTGCTCGGAGGGTTTGGATTCGGCCAAAGTAGCCCCGTTCAAGGGCAACATGGAT
GTCGCGGGCCTGGAGAAACTCATTGCTGAGCACGGGGACGACATTGCTGCGATCGTCATGACCGTGACCAACAACTCGGT
CGGCGGTCAGCCGGTTTCTCTGCAGAACATGCGAGAGACCTACGAGGTGGCTCACAAGTACGCAATTCCAGTCTGTCTTG
ACGCTGCTCGCTATGCCGAGAACGCCTATTTCATCCATGAGCGCGAGGAGGGATGTCAGGACCGTTCGTTACGCGATATC
GTCGCGGAGATGTTCCGCTACGGCGACATGTTCGTCATGAGCGCGAAGAAGGACGCCATCGTCAACATGGGTGGTTTGCT
CGGCGTCCGCGAAGATGGCGAACTAGCTGAGAAGGTCAAGAGCAACGTCATCTCCTTTGAGGGCTACCTTACCTACGGGG
GATTGGCAGGTCGCGACTTGGAAGCGTTGGCCGTTGGCCTCGAGGAGGGACTCGACATGGACTGGCAGCGGTACCGTGTC
GGCCAAATCCAATACCTTGGCGACCAGCTCGAGGAGGCTGGCGTGCCTTTCCAGAGCCCTGTTGGCGGTCATGCAGTATT
CATCGACGCCGGTCGCATGCTGCCGCATTTGGAGTGGAAACAGTACCCAGGGCACGCGCTCGCTACCCAACTGTACTTGG
ATGCAGGAATTCGATCCTGCGACATCGGTTCTTACCTTATGGACCGGGACCCCGTTACCCAGGAGGAGCAACAGGCGCCC
TTCGAGTTCACTCGGCTAGCAGTGCCGCGACGGGTCTACACCCAGTCTCACATGGACGTCGTCGCCGAGGCCGTTACCAA
GATTGCCCGGGAGCCTGAAAAAGTACCCGGATACCGCATTACATGGGAGCCTCCGGTCTTGCGCCATTTCACAAGTCGAC
TCGAACCAATTCGGTGA

Upstream 100 bases:

>100_bases
ATGATGCGAGGTGAGAGGATTGCAAAATCCGTTGAGGGAATCAACGGAAATTGGTCAACATCCGTTCAGCAGGTATTACA
GGCCCACGAAGGAGTGTGCA

Downstream 100 bases:

>100_bases
TGAGATGTCCACCCGGCTGGGAGGCTCTTCCCCGGCCGCTGGGTGCCCCGCCGTACCGTCACGCTTCGGTGCGGCGGGAT
CCGGAGCGCCGAGTGGAACA

Product: tryptophanase

Products: NA

Alternate protein names: L-tryptophan indole-lyase; TNase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKYIPEPFKIKMVEPIAMTSREERANILARAHYNMFGLPAESVYIDLLTDSGTGAMSDAQWAAVMRGDEAYSGGRSYMHL
MDVAGSIFGYSYIQPVHQGRAAEKVLMPLLVSRPGQLVVANTFFDTTRAHVGLAGGRPVDNVCSEGLDSAKVAPFKGNMD
VAGLEKLIAEHGDDIAAIVMTVTNNSVGGQPVSLQNMRETYEVAHKYAIPVCLDAARYAENAYFIHEREEGCQDRSLRDI
VAEMFRYGDMFVMSAKKDAIVNMGGLLGVREDGELAEKVKSNVISFEGYLTYGGLAGRDLEALAVGLEEGLDMDWQRYRV
GQIQYLGDQLEEAGVPFQSPVGGHAVFIDAGRMLPHLEWKQYPGHALATQLYLDAGIRSCDIGSYLMDRDPVTQEEQQAP
FEFTRLAVPRRVYTQSHMDVVAEAVTKIAREPEKVPGYRITWEPPVLRHFTSRLEPIR

Sequences:

>Translated_458_residues
MKYIPEPFKIKMVEPIAMTSREERANILARAHYNMFGLPAESVYIDLLTDSGTGAMSDAQWAAVMRGDEAYSGGRSYMHL
MDVAGSIFGYSYIQPVHQGRAAEKVLMPLLVSRPGQLVVANTFFDTTRAHVGLAGGRPVDNVCSEGLDSAKVAPFKGNMD
VAGLEKLIAEHGDDIAAIVMTVTNNSVGGQPVSLQNMRETYEVAHKYAIPVCLDAARYAENAYFIHEREEGCQDRSLRDI
VAEMFRYGDMFVMSAKKDAIVNMGGLLGVREDGELAEKVKSNVISFEGYLTYGGLAGRDLEALAVGLEEGLDMDWQRYRV
GQIQYLGDQLEEAGVPFQSPVGGHAVFIDAGRMLPHLEWKQYPGHALATQLYLDAGIRSCDIGSYLMDRDPVTQEEQQAP
FEFTRLAVPRRVYTQSHMDVVAEAVTKIAREPEKVPGYRITWEPPVLRHFTSRLEPIR
>Mature_458_residues
MKYIPEPFKIKMVEPIAMTSREERANILARAHYNMFGLPAESVYIDLLTDSGTGAMSDAQWAAVMRGDEAYSGGRSYMHL
MDVAGSIFGYSYIQPVHQGRAAEKVLMPLLVSRPGQLVVANTFFDTTRAHVGLAGGRPVDNVCSEGLDSAKVAPFKGNMD
VAGLEKLIAEHGDDIAAIVMTVTNNSVGGQPVSLQNMRETYEVAHKYAIPVCLDAARYAENAYFIHEREEGCQDRSLRDI
VAEMFRYGDMFVMSAKKDAIVNMGGLLGVREDGELAEKVKSNVISFEGYLTYGGLAGRDLEALAVGLEEGLDMDWQRYRV
GQIQYLGDQLEEAGVPFQSPVGGHAVFIDAGRMLPHLEWKQYPGHALATQLYLDAGIRSCDIGSYLMDRDPVTQEEQQAP
FEFTRLAVPRRVYTQSHMDVVAEAVTKIAREPEKVPGYRITWEPPVLRHFTSRLEPIR

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family [H]

Homologues:

Organism=Escherichia coli, GI87082323, Length=469, Percent_Identity=46.0554371002132, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- InterPro:   IPR018176 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: =4.1.99.1 [H]

Molecular weight: Translated: 50783; Mature: 50783

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00853 BETA_ELIM_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIPEPFKIKMVEPIAMTSREERANILARAHYNMFGLPAESVYIDLLTDSGTGAMSDAQ
CCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCEECCCCCCEEEEEEECCCCCCCCCCH
WAAVMRGDEAYSGGRSYMHLMDVAGSIFGYSYIQPVHQGRAAEKVLMPLLVSRPGQLVVA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEE
NTFFDTTRAHVGLAGGRPVDNVCSEGLDSAKVAPFKGNMDVAGLEKLIAEHGDDIAAIVM
EEHHHHHHHHEECCCCCCHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHCCCCEEEEEE
TVTNNSVGGQPVSLQNMRETYEVAHKYAIPVCLDAARYAENAYFIHEREEGCQDRSLRDI
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHH
VAEMFRYGDMFVMSAKKDAIVNMGGLLGVREDGELAEKVKSNVISFEGYLTYGGLAGRDL
HHHHHHCCCEEEEECCCCHHEECCCEEEECCCHHHHHHHHHCEEEEEEEEEECCCCCCCH
EALAVGLEEGLDMDWQRYRVGQIQYLGDQLEEAGVPFQSPVGGHAVFIDAGRMLPHLEWK
HHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHH
QYPGHALATQLYLDAGIRSCDIGSYLMDRDPVTQEEQQAPFEFTRLAVPRRVYTQSHMDV
CCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHCCCHHHHHHHCCHHHHHHHHHHH
VAEAVTKIAREPEKVPGYRITWEPPVLRHFTSRLEPIR
HHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKYIPEPFKIKMVEPIAMTSREERANILARAHYNMFGLPAESVYIDLLTDSGTGAMSDAQ
CCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCEECCCCCCEEEEEEECCCCCCCCCCH
WAAVMRGDEAYSGGRSYMHLMDVAGSIFGYSYIQPVHQGRAAEKVLMPLLVSRPGQLVVA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEE
NTFFDTTRAHVGLAGGRPVDNVCSEGLDSAKVAPFKGNMDVAGLEKLIAEHGDDIAAIVM
EEHHHHHHHHEECCCCCCHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHCCCCEEEEEE
TVTNNSVGGQPVSLQNMRETYEVAHKYAIPVCLDAARYAENAYFIHEREEGCQDRSLRDI
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHH
VAEMFRYGDMFVMSAKKDAIVNMGGLLGVREDGELAEKVKSNVISFEGYLTYGGLAGRDL
HHHHHHCCCEEEEECCCCHHEECCCEEEECCCHHHHHHHHHCEEEEEEEEEECCCCCCCH
EALAVGLEEGLDMDWQRYRVGQIQYLGDQLEEAGVPFQSPVGGHAVFIDAGRMLPHLEWK
HHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHH
QYPGHALATQLYLDAGIRSCDIGSYLMDRDPVTQEEQQAPFEFTRLAVPRRVYTQSHMDV
CCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHCCCHHHHHHHCCHHHHHHHHHHH
VAEAVTKIAREPEKVPGYRITWEPPVLRHFTSRLEPIR
HHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]