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The map label for this gene is rimO

Identifier: 50842489

GI number: 50842489

Start: 1090492

End: 1091937

Strand: Direct

Name: rimO

Synonym: PPA1006

Alternate gene names: 50842489

Gene position: 1090492-1091937 (Clockwise)

Preceding gene: 50842487

Following gene: 50842490

Centisome position: 42.59

GC content: 60.58

Gene sequence:

>1446_bases
GTGTCTGCCGCTGATCACCTCATGACAGTCCATCTAGTGTCGATGGGCTGCGCCCGCAACGACGTCGACTCCGAGGAGCT
TGCGGCCCGAATGGAGGCTGGTGGGTTCCGCCTCGTCGACGACCCTGCTGAGGCGGAGACCGTTGTCGTCAATACTTGTG
GCTTCATCGAGCAGGCCAAGAAGGATTCCGTCGATACCTTGCTGGCTGCCGCCGATCTCAAGGGGAATGGCATCACGACC
TCAGTCGTTGCGGTTGGGTGCATGGCCGAGCGTTACGGGCGTGAGCTCGCGGAGTCTTTGCCTGAGGCTGACGCGGTGCT
GGGTTTTGACGATTACGGCGACATCGCGGGTCGGTTGCGAACCATCTTGGACGGCGGATCGCTTGAGACCCATGTTCCCC
GTGACCGACGTACCCTGTTGCCGATTAGTCCGGTCGATCGACCAACCGCACGCGCTGAGGTTTCTGTTCCCGGTCACGGC
ACGGCCCCCGACCTTTCAGCCTCCGTGACTCCGGATTCCGGCCCCCGAGCCACTCGGCGACGGCTCGGGACAGGGCCCAG
TGCACCGCTGAAGATGGCCTCGGGCTGTGACCGTCGTTGTGCGTTCTGTGCCATCCCGCGTTTTCGCGGCTCTTACCTTT
CTCGCCCGATCGCAGAAATCGTCGAGGAGGCTCGTTGGTTGGTGGACCACGGCGTCAAGGAAGTATTCCTCGTCAGCGAG
AATTCCAGTTCCTATGGTAAAGATTTAGGAGATCTCAGGCTGTTGGAGAAGTTGCTCGTCAACCTTGATCAGGTTGATGG
TCTGGAATGGATCCGAGTGTCTTATTTGCAGCCAGCAGAGCTGCGACCGGGGCTTATTGACACGATATTGGCCACCGACA
AGGTCGTGCCCTATTTCGATCTGTCCTTCCAACACGCGTCGGGACCGTTACTGCGTCGCATGCGTCGATTCGGTGACGCC
GAGTCGTTTCTGAATATCATCGATTCCATTCGCTCCCGCTGCCCGGAGGCCGGTTTGAGGTCGAACTTCATTACCGGCTT
CCCTGGTGAGACCGATGCGGACGTCGCAGTTTTGGCTGACTTTTTACAGCGCGCCCGCCTCGATGTTGCAGGCGTCTTCG
CCTACTCCGACGAGGAGGGCACCGAGGCCGCCGGACTTGATGGACACGTAGACGAGGACGTCGTCACTGCTCGTCGCGAA
GACCTCGCTGATCTGACCGACGAACTCGTTTCCCAGCGTGCTGAGGACCGGATTGGAACCCGGGGGCGGGTGATGGTCGA
GGAGATCGATGAGGCTGTTATCGGCAGGGCCGAACATCAGGGGCCGGAAGTCGATGGCTGTGTCACCCTGGTCGACGCAG
CCGCAGTGAGCGTGGGGGATATCGTCGACGTCGAGTTCGTCGGTTCTGACGGTGTTGATCTCGTCGCGCGGCCTGCGAAT
GCCTAA

Upstream 100 bases:

>100_bases
GTCAATGGCGTTCTTGTGGTGGTCGGTCTTGCGGAAGGTCATGGATCCTACGGTAGACGCGTAGGATGCAGTCGAAACCA
CCTCGTCCATCAGGAGTCTC

Downstream 100 bases:

>100_bases
GCCTCGCGACTAGCCGTCGATCTCAGCGCGCTGCCAAAAAGTGATCAATCCGAGTCGCTTCCAGGGCGGTGAGTCGCATG
AGGCATCGTCACGCGACATA

Product: radical SAM superfamily protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSAADHLMTVHLVSMGCARNDVDSEELAARMEAGGFRLVDDPAEAETVVVNTCGFIEQAKKDSVDTLLAAADLKGNGITT
SVVAVGCMAERYGRELAESLPEADAVLGFDDYGDIAGRLRTILDGGSLETHVPRDRRTLLPISPVDRPTARAEVSVPGHG
TAPDLSASVTPDSGPRATRRRLGTGPSAPLKMASGCDRRCAFCAIPRFRGSYLSRPIAEIVEEARWLVDHGVKEVFLVSE
NSSSYGKDLGDLRLLEKLLVNLDQVDGLEWIRVSYLQPAELRPGLIDTILATDKVVPYFDLSFQHASGPLLRRMRRFGDA
ESFLNIIDSIRSRCPEAGLRSNFITGFPGETDADVAVLADFLQRARLDVAGVFAYSDEEGTEAAGLDGHVDEDVVTARRE
DLADLTDELVSQRAEDRIGTRGRVMVEEIDEAVIGRAEHQGPEVDGCVTLVDAAAVSVGDIVDVEFVGSDGVDLVARPAN
A

Sequences:

>Translated_481_residues
MSAADHLMTVHLVSMGCARNDVDSEELAARMEAGGFRLVDDPAEAETVVVNTCGFIEQAKKDSVDTLLAAADLKGNGITT
SVVAVGCMAERYGRELAESLPEADAVLGFDDYGDIAGRLRTILDGGSLETHVPRDRRTLLPISPVDRPTARAEVSVPGHG
TAPDLSASVTPDSGPRATRRRLGTGPSAPLKMASGCDRRCAFCAIPRFRGSYLSRPIAEIVEEARWLVDHGVKEVFLVSE
NSSSYGKDLGDLRLLEKLLVNLDQVDGLEWIRVSYLQPAELRPGLIDTILATDKVVPYFDLSFQHASGPLLRRMRRFGDA
ESFLNIIDSIRSRCPEAGLRSNFITGFPGETDADVAVLADFLQRARLDVAGVFAYSDEEGTEAAGLDGHVDEDVVTARRE
DLADLTDELVSQRAEDRIGTRGRVMVEEIDEAVIGRAEHQGPEVDGCVTLVDAAAVSVGDIVDVEFVGSDGVDLVARPAN
A
>Mature_480_residues
SAADHLMTVHLVSMGCARNDVDSEELAARMEAGGFRLVDDPAEAETVVVNTCGFIEQAKKDSVDTLLAAADLKGNGITTS
VVAVGCMAERYGRELAESLPEADAVLGFDDYGDIAGRLRTILDGGSLETHVPRDRRTLLPISPVDRPTARAEVSVPGHGT
APDLSASVTPDSGPRATRRRLGTGPSAPLKMASGCDRRCAFCAIPRFRGSYLSRPIAEIVEEARWLVDHGVKEVFLVSEN
SSSYGKDLGDLRLLEKLLVNLDQVDGLEWIRVSYLQPAELRPGLIDTILATDKVVPYFDLSFQHASGPLLRRMRRFGDAE
SFLNIIDSIRSRCPEAGLRSNFITGFPGETDADVAVLADFLQRARLDVAGVFAYSDEEGTEAAGLDGHVDEDVVTARRED
LADLTDELVSQRAEDRIGTRGRVMVEEIDEAVIGRAEHQGPEVDGCVTLVDAAAVSVGDIVDVEFVGSDGVDLVARPANA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=447, Percent_Identity=25.9507829977629, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI28872782, Length=447, Percent_Identity=25.9507829977629, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI93277076, Length=485, Percent_Identity=22.4742268041237, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1787057, Length=485, Percent_Identity=33.8144329896907, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1786882, Length=460, Percent_Identity=26.5217391304348, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71996771, Length=300, Percent_Identity=28, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922432, Length=443, Percent_Identity=24.3792325056433, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_PROAC (Q6A908)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055716.1
- ProteinModelPortal:   Q6A908
- SMR:   Q6A908
- GeneID:   2932809
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1006
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK902972
- BioCyc:   PACN267747:PPA1006-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51717; Mature: 51585

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAADHLMTVHLVSMGCARNDVDSEELAARMEAGGFRLVDDPAEAETVVVNTCGFIEQAK
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCCEEEEECHHHHHHHH
KDSVDTLLAAADLKGNGITTSVVAVGCMAERYGRELAESLPEADAVLGFDDYGDIAGRLR
HCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHH
TILDGGSLETHVPRDRRTLLPISPVDRPTARAEVSVPGHGTAPDLSASVTPDSGPRATRR
HHHCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHH
RLGTGPSAPLKMASGCDRRCAFCAIPRFRGSYLSRPIAEIVEEARWLVDHGVKEVFLVSE
HCCCCCCCCHHHHCCCCCCHHEEECCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEC
NSSSYGKDLGDLRLLEKLLVNLDQVDGLEWIRVSYLQPAELRPGLIDTILATDKVVPYFD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCHHHHHHHHCCCCCEEE
LSFQHASGPLLRRMRRFGDAESFLNIIDSIRSRCPEAGLRSNFITGFPGETDADVAVLAD
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
FLQRARLDVAGVFAYSDEEGTEAAGLDGHVDEDVVTARREDLADLTDELVSQRAEDRIGT
HHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RGRVMVEEIDEAVIGRAEHQGPEVDGCVTLVDAAAVSVGDIVDVEFVGSDGVDLVARPAN
CCCHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCEEECCCC
A
C
>Mature Secondary Structure 
SAADHLMTVHLVSMGCARNDVDSEELAARMEAGGFRLVDDPAEAETVVVNTCGFIEQAK
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCCEEEEECHHHHHHHH
KDSVDTLLAAADLKGNGITTSVVAVGCMAERYGRELAESLPEADAVLGFDDYGDIAGRLR
HCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHH
TILDGGSLETHVPRDRRTLLPISPVDRPTARAEVSVPGHGTAPDLSASVTPDSGPRATRR
HHHCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHH
RLGTGPSAPLKMASGCDRRCAFCAIPRFRGSYLSRPIAEIVEEARWLVDHGVKEVFLVSE
HCCCCCCCCHHHHCCCCCCHHEEECCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEC
NSSSYGKDLGDLRLLEKLLVNLDQVDGLEWIRVSYLQPAELRPGLIDTILATDKVVPYFD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCHHHHHHHHCCCCCEEE
LSFQHASGPLLRRMRRFGDAESFLNIIDSIRSRCPEAGLRSNFITGFPGETDADVAVLAD
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
FLQRARLDVAGVFAYSDEEGTEAAGLDGHVDEDVVTARREDLADLTDELVSQRAEDRIGT
HHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RGRVMVEEIDEAVIGRAEHQGPEVDGCVTLVDAAAVSVGDIVDVEFVGSDGVDLVARPAN
CCCHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCEEECCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA