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The map label for this gene is dus [H]

Identifier: 50842438

GI number: 50842438

Start: 1036576

End: 1037811

Strand: Direct

Name: dus [H]

Synonym: PPA0954

Alternate gene names: 50842438

Gene position: 1036576-1037811 (Clockwise)

Preceding gene: 50842436

Following gene: 50842439

Centisome position: 40.49

GC content: 61.25

Gene sequence:

>1236_bases
GTGGCAATGCGGGTCGTGATTTCTCGTTGTGCCACTGGCGCTGGCACGACGGTTTCTGCTCAACCGGATTGCCACGCTTA
CCATGGGCTGGTGTTTTCCGATCCCCTGACCTTGATCGCGCCCTCTGGTGGCACCGTTTCGGTAGATACCCCGGTGGTGC
TGGCCCCCATGGCCGGCGTTACCAATGCCGCATTCCGCCAGCTTTGTGCCGAACAAGGTGCGGGCCTGTATGTCTGCGAA
ATGATCACGTCGCGTGGCCTGGTTTACGGGGACCACAAGACCCACGGCATGCTTGCCTTTGCCGAGTGTGAAGATGTCCG
GTCCGTTCAGCTCTATGGCGTCGACCCGGGAATGGTGGGCGAGGCGACATCGATCCTGTGCGAGAAGTACGGAGTCAATC
ACGTCGACCTTAATTTCGGCTGTCCAGTGCCCAAGGTCACCCGCAAGGGTGGTGGGGGAGTCCTGCCGTGGAAGCTCGAC
CGGTTTGCGGCCGTGGTTTCTGCGGCTGTCCAGGCCGGTGACGCCCATGGGGTCCCGGTCACCGTCAAGACTCGGATCGG
CATTGACCCCGAGCACATCACCTACATTGATTCAGCCCGGGCTGCGGTCGATTCCGGCGCCGCTGCGGTGTGTTTGCACG
CTCGCACTGTCGCCGAGGCCTACGCTGGGCACTCCCACTGGGATGCCATTGGCGAGCTTGTCAAGACCGTCGATGTCCCC
GTCATTGGAAACGGTGACATCTGGGAGGCCAGCGACGCAATGGCCATGGTCGGGCAAACGGGTTGTGCCGGCGTCGAGGT
TGGTCGTGGTTGTCTCGGTCGTCCGTGGCTGTTCCGTGACTTGGCCGATGCCTTCGCCGGTCGTGACACCACCACCTTGC
CGACCTTGGGTGAAGTGTGCGCCATGATCCGTCGCCATGCCGAACTGTTGGTGAAATATCAGGGAATGCATGGTCTGGTG
GACATGCGCAAACATATGGCTTGGTACCTTAAGGGATTCCCCGTAGGTGGGGAAACCCGTCATGTTCTCGGCCAGATTTC
CAGTTTCGACGAACTCGATTCCTTGTTGGCCACCCTTGACCACGACGCCCCTTTCCCAGTTAAGGAGCTTGGCAAGGCCC
GTGGTCGTCAGGGCGCCCCGCGGCGAAAGGTCGTCATGCCGCACGGATGGCTGGACACTCACACCCTTGACGACGATGAT
CTGTCCGCTGCTGAGATCGACGCTTCCGGGGGATGA

Upstream 100 bases:

>100_bases
ATAGGGCGGTCCCAGCGCCGGCTGGTAGATGGGTTCTCGTCAGGGATGATCACAGCATGAGGGTAGTGCTCGGCCCGCAG
CGGCCTGTGGCCGGGATGGT

Downstream 100 bases:

>100_bases
TCTCGCCTCGGGCTGGTCCCGCGACTCTGGTTAAGACAGTATAGGGACATGTCCGAAGTGGGAGAACGCGCTCGAGCCTG
GGCTCTGACAATGGCTGGCC

Product: NifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MAMRVVISRCATGAGTTVSAQPDCHAYHGLVFSDPLTLIAPSGGTVSVDTPVVLAPMAGVTNAAFRQLCAEQGAGLYVCE
MITSRGLVYGDHKTHGMLAFAECEDVRSVQLYGVDPGMVGEATSILCEKYGVNHVDLNFGCPVPKVTRKGGGGVLPWKLD
RFAAVVSAAVQAGDAHGVPVTVKTRIGIDPEHITYIDSARAAVDSGAAAVCLHARTVAEAYAGHSHWDAIGELVKTVDVP
VIGNGDIWEASDAMAMVGQTGCAGVEVGRGCLGRPWLFRDLADAFAGRDTTTLPTLGEVCAMIRRHAELLVKYQGMHGLV
DMRKHMAWYLKGFPVGGETRHVLGQISSFDELDSLLATLDHDAPFPVKELGKARGRQGAPRRKVVMPHGWLDTHTLDDDD
LSAAEIDASGG

Sequences:

>Translated_411_residues
MAMRVVISRCATGAGTTVSAQPDCHAYHGLVFSDPLTLIAPSGGTVSVDTPVVLAPMAGVTNAAFRQLCAEQGAGLYVCE
MITSRGLVYGDHKTHGMLAFAECEDVRSVQLYGVDPGMVGEATSILCEKYGVNHVDLNFGCPVPKVTRKGGGGVLPWKLD
RFAAVVSAAVQAGDAHGVPVTVKTRIGIDPEHITYIDSARAAVDSGAAAVCLHARTVAEAYAGHSHWDAIGELVKTVDVP
VIGNGDIWEASDAMAMVGQTGCAGVEVGRGCLGRPWLFRDLADAFAGRDTTTLPTLGEVCAMIRRHAELLVKYQGMHGLV
DMRKHMAWYLKGFPVGGETRHVLGQISSFDELDSLLATLDHDAPFPVKELGKARGRQGAPRRKVVMPHGWLDTHTLDDDD
LSAAEIDASGG
>Mature_410_residues
AMRVVISRCATGAGTTVSAQPDCHAYHGLVFSDPLTLIAPSGGTVSVDTPVVLAPMAGVTNAAFRQLCAEQGAGLYVCEM
ITSRGLVYGDHKTHGMLAFAECEDVRSVQLYGVDPGMVGEATSILCEKYGVNHVDLNFGCPVPKVTRKGGGGVLPWKLDR
FAAVVSAAVQAGDAHGVPVTVKTRIGIDPEHITYIDSARAAVDSGAAAVCLHARTVAEAYAGHSHWDAIGELVKTVDVPV
IGNGDIWEASDAMAMVGQTGCAGVEVGRGCLGRPWLFRDLADAFAGRDTTTLPTLGEVCAMIRRHAELLVKYQGMHGLVD
MRKHMAWYLKGFPVGGETRHVLGQISSFDELDSLLATLDHDAPFPVKELGKARGRQGAPRRKVVMPHGWLDTHTLDDDDL
SAAEIDASGG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=273, Percent_Identity=31.1355311355311, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI239788483, Length=234, Percent_Identity=33.7606837606838, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI31742496, Length=309, Percent_Identity=25.5663430420712, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI40807366, Length=258, Percent_Identity=27.5193798449612, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789660, Length=323, Percent_Identity=34.3653250773994, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788462, Length=295, Percent_Identity=30.1694915254237, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=244, Percent_Identity=29.0983606557377, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=305, Percent_Identity=25.9016393442623, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=234, Percent_Identity=29.9145299145299, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323437, Length=254, Percent_Identity=29.1338582677165, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323433, Length=323, Percent_Identity=21.3622291021672, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585320, Length=273, Percent_Identity=30.4029304029304, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=27.7056277056277, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=27.7056277056277, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 43449; Mature: 43318

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMRVVISRCATGAGTTVSAQPDCHAYHGLVFSDPLTLIAPSGGTVSVDTPVVLAPMAGV
CHHHHHHHHHCCCCCCEEECCCCCHHHCCEEEECCEEEEECCCCEEEECCCEEEECCCCC
TNAAFRQLCAEQGAGLYVCEMITSRGLVYGDHKTHGMLAFAECEDVRSVQLYGVDPGMVG
HHHHHHHHHHHCCCCEEEEEHHHHCCEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCC
EATSILCEKYGVNHVDLNFGCPVPKVTRKGGGGVLPWKLDRFAAVVSAAVQAGDAHGVPV
HHHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCE
TVKTRIGIDPEHITYIDSARAAVDSGAAAVCLHARTVAEAYAGHSHWDAIGELVKTVDVP
EEEEECCCCHHHEEEEHHHHHHHCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
VIGNGDIWEASDAMAMVGQTGCAGVEVGRGCLGRPWLFRDLADAFAGRDTTTLPTLGEVC
EECCCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHH
AMIRRHAELLVKYQGMHGLVDMRKHMAWYLKGFPVGGETRHVLGQISSFDELDSLLATLD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHC
HDAPFPVKELGKARGRQGAPRRKVVMPHGWLDTHTLDDDDLSAAEIDASGG
CCCCCCHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEECCCCC
>Mature Secondary Structure 
AMRVVISRCATGAGTTVSAQPDCHAYHGLVFSDPLTLIAPSGGTVSVDTPVVLAPMAGV
HHHHHHHHHCCCCCCEEECCCCCHHHCCEEEECCEEEEECCCCEEEECCCEEEECCCCC
TNAAFRQLCAEQGAGLYVCEMITSRGLVYGDHKTHGMLAFAECEDVRSVQLYGVDPGMVG
HHHHHHHHHHHCCCCEEEEEHHHHCCEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCC
EATSILCEKYGVNHVDLNFGCPVPKVTRKGGGGVLPWKLDRFAAVVSAAVQAGDAHGVPV
HHHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCE
TVKTRIGIDPEHITYIDSARAAVDSGAAAVCLHARTVAEAYAGHSHWDAIGELVKTVDVP
EEEEECCCCHHHEEEEHHHHHHHCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
VIGNGDIWEASDAMAMVGQTGCAGVEVGRGCLGRPWLFRDLADAFAGRDTTTLPTLGEVC
EECCCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHH
AMIRRHAELLVKYQGMHGLVDMRKHMAWYLKGFPVGGETRHVLGQISSFDELDSLLATLD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHC
HDAPFPVKELGKARGRQGAPRRKVVMPHGWLDTHTLDDDDLSAAEIDASGG
CCCCCCHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]