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The map label for this gene is folC [H]

Identifier: 50842308

GI number: 50842308

Start: 901620

End: 903065

Strand: Direct

Name: folC [H]

Synonym: PPA0821

Alternate gene names: 50842308

Gene position: 901620-903065 (Clockwise)

Preceding gene: 50842307

Following gene: 50842309

Centisome position: 35.22

GC content: 60.44

Gene sequence:

>1446_bases
TTGAGCAGCCATAATCAGCTTGTCGCTGAGTTGACGGCTCGCTGGCCGGAGCATCGCATCGGCCCCGGTTTGGATCGGGA
GCGAGCCGTCCTCGACCTGCTCGGTAACCCACAGCAGGCCTGTCCCGTCATCCAGATTGCCGGTACTAACGGCAAGGGAT
CGACGGCGATCATGGTTGATTCTTTGCTGCGGTCGGCTGGTCTGCGGGTAGGTCGATACTGCTCCCCGCACCTGGTGGAC
GTCACTGAGCGCATCTGCATCGATGGTCGTCCCGTCAGCCACGATCTCTTTGACGAGACGTGGCGTCAAGTTGAGCCCAT
GGTGGGCCTGGTTGACGCTCAGCACATCGATGGCATCGAGATGACCTTCTTTGAGGTGATGACAGCGATGGCCTTCGCCG
CCTTCGCCGATGCGCCGGTGGACGTCGCCGTCGTTGAGGTGGGCTTAGGGGGACGCTGGGACGCGACCAATGTCGCTGAT
GCGAACATCGCTGTTGTTACCCCCGTTGCCATGGACCATATGCATATTCTCGGCGATAGTCTCGACAAGATCGCTGCCGA
GAAGGCCGGGATTATTAAGCCTGGGTGCACCCCCGTCATTGCTGGTCAGGAGGCGGAAGCTGCTCCGGTGTTGTTAGCTC
AGTGTGCCGATGCCGGCGTCAAGCCCTTGTTGGAGGGCCCAGACTGGGGTTTATTGGATCGACGTAGCGCCATCGGGGGT
CAAGTCCTTCGCATCCAGACGGCATCTGGGCCTCTAGGAGAGTTGTTCTTGCCGGTTTTTGGCGAGCATATGGCCCACAA
TGCTGCTCAGGCGGTGGCAGCTGTGGAAGCTCTCACTGGGCCGCTTAAGCCCGCAATTATTGAGCAGGGGCTGGCAGCTG
TGCAGGCTCCGGCTCGCCTTGAAGTAGTGCGACGTTCTCCGCTAGTTATCCTCGATACCTTCCACAATCCGCACGGCGCC
GCCTCCGCTATGGCGGGAGTGCGCGAGTCGTTCGAGGTTGAGCCCTTAATCGCCGTTGTGGCAGCGATGGCGGACAAAGA
CGTCGACGGTGTGCTGGCGGTGCTTTCGGATGCGGTGAGCTACCTTGTCGTCACCTCAATGCCAGATCTTCCGCGCGCTC
TGAGCGTTGACGAGCTAGCTGACAAGGCGTCAGCCCATTGGGATTCTGATCATCTCACCCGCGTTACTGACGTCCCCGAA
GCGCTCGAGGAAGCTTTCCGCATCGCGGATTCCTGTGGTCCTGGGGCTGGGGTTCTTATTGCCGGATCGGTGGTTTTGGC
GGGTCGGGCTCGAGCCATCCTTCGCCCGGATGGCACCATGCTGGATGCTCCACAGACCGCCATTGTTGAGACCCCGGATC
TATCTGAGGATCAGGTCAAAGCCATGGAGGGGCATCAGCTCGAGCCATTGCCTGACGATGATATGAGCGACGACGGGGGC
TGGTGA

Upstream 100 bases:

>100_bases
CCTACGTAAAAGAGCGTCGCAGCGAGGCCGAGGCCGCTGCGATTCTTGCAGAAGCATTGGAGAACCTGCGTCAACGTCGC
CGTGATGGTGAGGTCATCTA

Downstream 100 bases:

>100_bases
CGTTGGTCGCGCATCCCGGGGCGGGGAAGGGTGGAATCCTCATTCTTCCTGCCGGTAACCCGATGACGAAGGTTGTGGTG
AGCTTCCTGGCCTTTGAGGT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSSHNQLVAELTARWPEHRIGPGLDRERAVLDLLGNPQQACPVIQIAGTNGKGSTAIMVDSLLRSAGLRVGRYCSPHLVD
VTERICIDGRPVSHDLFDETWRQVEPMVGLVDAQHIDGIEMTFFEVMTAMAFAAFADAPVDVAVVEVGLGGRWDATNVAD
ANIAVVTPVAMDHMHILGDSLDKIAAEKAGIIKPGCTPVIAGQEAEAAPVLLAQCADAGVKPLLEGPDWGLLDRRSAIGG
QVLRIQTASGPLGELFLPVFGEHMAHNAAQAVAAVEALTGPLKPAIIEQGLAAVQAPARLEVVRRSPLVILDTFHNPHGA
ASAMAGVRESFEVEPLIAVVAAMADKDVDGVLAVLSDAVSYLVVTSMPDLPRALSVDELADKASAHWDSDHLTRVTDVPE
ALEEAFRIADSCGPGAGVLIAGSVVLAGRARAILRPDGTMLDAPQTAIVETPDLSEDQVKAMEGHQLEPLPDDDMSDDGG
W

Sequences:

>Translated_481_residues
MSSHNQLVAELTARWPEHRIGPGLDRERAVLDLLGNPQQACPVIQIAGTNGKGSTAIMVDSLLRSAGLRVGRYCSPHLVD
VTERICIDGRPVSHDLFDETWRQVEPMVGLVDAQHIDGIEMTFFEVMTAMAFAAFADAPVDVAVVEVGLGGRWDATNVAD
ANIAVVTPVAMDHMHILGDSLDKIAAEKAGIIKPGCTPVIAGQEAEAAPVLLAQCADAGVKPLLEGPDWGLLDRRSAIGG
QVLRIQTASGPLGELFLPVFGEHMAHNAAQAVAAVEALTGPLKPAIIEQGLAAVQAPARLEVVRRSPLVILDTFHNPHGA
ASAMAGVRESFEVEPLIAVVAAMADKDVDGVLAVLSDAVSYLVVTSMPDLPRALSVDELADKASAHWDSDHLTRVTDVPE
ALEEAFRIADSCGPGAGVLIAGSVVLAGRARAILRPDGTMLDAPQTAIVETPDLSEDQVKAMEGHQLEPLPDDDMSDDGG
W
>Mature_480_residues
SSHNQLVAELTARWPEHRIGPGLDRERAVLDLLGNPQQACPVIQIAGTNGKGSTAIMVDSLLRSAGLRVGRYCSPHLVDV
TERICIDGRPVSHDLFDETWRQVEPMVGLVDAQHIDGIEMTFFEVMTAMAFAAFADAPVDVAVVEVGLGGRWDATNVADA
NIAVVTPVAMDHMHILGDSLDKIAAEKAGIIKPGCTPVIAGQEAEAAPVLLAQCADAGVKPLLEGPDWGLLDRRSAIGGQ
VLRIQTASGPLGELFLPVFGEHMAHNAAQAVAAVEALTGPLKPAIIEQGLAAVQAPARLEVVRRSPLVILDTFHNPHGAA
SAMAGVRESFEVEPLIAVVAAMADKDVDGVLAVLSDAVSYLVVTSMPDLPRALSVDELADKASAHWDSDHLTRVTDVPEA
LEEAFRIADSCGPGAGVLIAGSVVLAGRARAILRPDGTMLDAPQTAIVETPDLSEDQVKAMEGHQLEPLPDDDMSDDGGW

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=350, Percent_Identity=30.5714285714286, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI66932984, Length=350, Percent_Identity=30.5714285714286, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1788654, Length=337, Percent_Identity=35.3115727002967, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17553150, Length=207, Percent_Identity=38.6473429951691, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553148, Length=207, Percent_Identity=38.6473429951691, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71984923, Length=207, Percent_Identity=38.6473429951691, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=429, Percent_Identity=28.6713286713287, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6324815, Length=213, Percent_Identity=31.924882629108, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24641571, Length=182, Percent_Identity=37.9120879120879, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24581568, Length=184, Percent_Identity=38.0434782608696, Blast_Score=113, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 50651; Mature: 50520

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHNQLVAELTARWPEHRIGPGLDRERAVLDLLGNPQQACPVIQIAGTNGKGSTAIMVD
CCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHCCEEEEECCCCCCCHHHHHH
SLLRSAGLRVGRYCSPHLVDVTERICIDGRPVSHDLFDETWRQVEPMVGLVDAQHIDGIE
HHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
MTFFEVMTAMAFAAFADAPVDVAVVEVGLGGRWDATNVADANIAVVTPVAMDHMHILGDS
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
LDKIAAEKAGIIKPGCTPVIAGQEAEAAPVLLAQCADAGVKPLLEGPDWGLLDRRSAIGG
HHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCC
QVLRIQTASGPLGELFLPVFGEHMAHNAAQAVAAVEALTGPLKPAIIEQGLAAVQAPARL
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHH
EVVRRSPLVILDTFHNPHGAASAMAGVRESFEVEPLIAVVAAMADKDVDGVLAVLSDAVS
HHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
YLVVTSMPDLPRALSVDELADKASAHWDSDHLTRVTDVPEALEEAFRIADSCGPGAGVLI
HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
AGSVVLAGRARAILRPDGTMLDAPQTAIVETPDLSEDQVKAMEGHQLEPLPDDDMSDDGG
ECCEEEECCCCEEECCCCCEECCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
W
C
>Mature Secondary Structure 
SSHNQLVAELTARWPEHRIGPGLDRERAVLDLLGNPQQACPVIQIAGTNGKGSTAIMVD
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHCCEEEEECCCCCCCHHHHHH
SLLRSAGLRVGRYCSPHLVDVTERICIDGRPVSHDLFDETWRQVEPMVGLVDAQHIDGIE
HHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
MTFFEVMTAMAFAAFADAPVDVAVVEVGLGGRWDATNVADANIAVVTPVAMDHMHILGDS
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
LDKIAAEKAGIIKPGCTPVIAGQEAEAAPVLLAQCADAGVKPLLEGPDWGLLDRRSAIGG
HHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCC
QVLRIQTASGPLGELFLPVFGEHMAHNAAQAVAAVEALTGPLKPAIIEQGLAAVQAPARL
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHH
EVVRRSPLVILDTFHNPHGAASAMAGVRESFEVEPLIAVVAAMADKDVDGVLAVLSDAVS
HHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
YLVVTSMPDLPRALSVDELADKASAHWDSDHLTRVTDVPEALEEAFRIADSCGPGAGVLI
HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
AGSVVLAGRARAILRPDGTMLDAPQTAIVETPDLSEDQVKAMEGHQLEPLPDDDMSDDGG
ECCEEEECCCCEEECCCCCEECCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
W
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]