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The map label for this gene is gcvH [H]

Identifier: 50842230

GI number: 50842230

Start: 816235

End: 816606

Strand: Reverse

Name: gcvH [H]

Synonym: PPA0743

Alternate gene names: 50842230

Gene position: 816606-816235 (Counterclockwise)

Preceding gene: 50842231

Following gene: 50842229

Centisome position: 31.9

GC content: 59.41

Gene sequence:

>372_bases
ATGGAACTGTCCGAACTTAAGTACTCCGCCGAGCATGAGTGGGTCGCCGTTGATGGTGACACCGTGACCGTAGGAATCAC
CGACTACGCCGCAGAGGCCCTGGGGGACGTCGTCTTTGTCGACGTCCCCCAGGTCGGCAGCGATGTTTCTGCCGGATCCG
CCATTGGCGAGGTCGAATCGACTAAATCGGTCTCCGACCTCTTTAGCCCCGTGACGGGCACCGTCTCGGAAGTTAATAAG
GCCATCACCGAGTCTCCCGAGCTTGTCAACTCCGATCCGTTCGGACAGGGATGGTTGTTCAAGGTTTCCGGTGGTCAGCT
GGGTGACGACCTCATGGACCGTGACGCCTACCTCAAGCTCACCGCCGAGTGA

Upstream 100 bases:

>100_bases
TGTGCGGGGCAAAGCCCTCAACACTACCGTCGTCGAATTACCGTTCTACAAGCGGTCCTGACCCCAACCACAATCCCCAC
ACAAATACCTAGGAGCAGAC

Downstream 100 bases:

>100_bases
TGATCATGACTGACCACGCTGAGAATCGGTGCGGCCTCGAAGGGCCGCGCCCTTTCTCGAGCCGCCACGTTGGATCCGTC
GAAGATGACCTGCGATACAT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 123

Protein sequence:

>123_residues
MELSELKYSAEHEWVAVDGDTVTVGITDYAAEALGDVVFVDVPQVGSDVSAGSAIGEVESTKSVSDLFSPVTGTVSEVNK
AITESPELVNSDPFGQGWLFKVSGGQLGDDLMDRDAYLKLTAE

Sequences:

>Translated_123_residues
MELSELKYSAEHEWVAVDGDTVTVGITDYAAEALGDVVFVDVPQVGSDVSAGSAIGEVESTKSVSDLFSPVTGTVSEVNK
AITESPELVNSDPFGQGWLFKVSGGQLGDDLMDRDAYLKLTAE
>Mature_123_residues
MELSELKYSAEHEWVAVDGDTVTVGITDYAAEALGDVVFVDVPQVGSDVSAGSAIGEVESTKSVSDLFSPVTGTVSEVNK
AITESPELVNSDPFGQGWLFKVSGGQLGDDLMDRDAYLKLTAE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=42.8571428571429, Blast_Score=105, Evalue=1e-23,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=42.8571428571429, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=50, Blast_Score=120, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=43.1034482758621, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=119, Percent_Identity=39.4957983193277, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=102, Percent_Identity=46.078431372549, Blast_Score=110, Evalue=7e-26,
Organism=Drosophila melanogaster, GI17865652, Length=116, Percent_Identity=43.1034482758621, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 12960; Mature: 12960

Theoretical pI: Translated: 3.67; Mature: 3.67

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSELKYSAEHEWVAVDGDTVTVGITDYAAEALGDVVFVDVPQVGSDVSAGSAIGEVES
CCHHHCCCCCCCCEEEECCCEEEEEEHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCC
TKSVSDLFSPVTGTVSEVNKAITESPELVNSDPFGQGWLFKVSGGQLGDDLMDRDAYLKL
HHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHCCCEEEEE
TAE
ECC
>Mature Secondary Structure
MELSELKYSAEHEWVAVDGDTVTVGITDYAAEALGDVVFVDVPQVGSDVSAGSAIGEVES
CCHHHCCCCCCCCEEEECCCEEEEEEHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCC
TKSVSDLFSPVTGTVSEVNKAITESPELVNSDPFGQGWLFKVSGGQLGDDLMDRDAYLKL
HHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHCCCEEEEE
TAE
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA