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The map label for this gene is lipB

Identifier: 50842180

GI number: 50842180

Start: 764092

End: 764910

Strand: Reverse

Name: lipB

Synonym: PPA0692

Alternate gene names: 50842180

Gene position: 764910-764092 (Counterclockwise)

Preceding gene: 50842181

Following gene: 50842179

Centisome position: 29.88

GC content: 61.05

Gene sequence:

>819_bases
ATGCAACAGGACCCTCCCACATCTCAGCCCCACACTCCACAGATAGTTGACGGGGTGAAGCCCCGCGGCTGGGTTGATCA
TCCCGCCGGACTACACTTCGAGTACCTGGGGATTGCAGATAGCCGTCCCACCCGCACTGAATACAACGAGTGCTGGGCGC
ATCAGCGCGAGGTGCACGCTGAGGTGTCCGCCCACCAGCGCCCCAACACAGTCATTTACGTCGAACATGATCCCGTCTAC
ACAGCCGGACGACGCACCCGCAAGGAGGCTTATCCTTTCGACGGCACCCCGGTCGTTCCGGTGGACCGGGGCGGTGAAAT
CACCTGGCATGGCCCTGGTCAGCTTGTCGGCTACCCGATTGTCTTCTTGCAGCGCGGTATCGGCGTTGTCGATTATGTGC
GCCGAGTAGAGGAAGCCGTTATCCGGCTCGTTTCCCAATACGGATTGCGTGCCGGGCGCGTCCCTGGACGAACCGGAGTG
TGGTTCCCGTCCGATGGAATGGGACCCGAGCGAAAAGTGTGCGCCATCGGCATCCGCGTGTCTCGACAGACAGCGATGCA
CGGTTTCGCCCTCAACATCGACCCCGACACTGCTGGCTTCGACAACATTATTCCGTGCGGCATCTCCGACGCCGACGTGA
CCAGCATGGCACGCGAACTAAGACGTCTGCACGGCCCCGACGCCGAGGTTCCCTCTTTGCTAGAGGTGGCTGGCAACCTC
GAGCCCATCCTCACTGAGATGATGTCGTTTCAGCCGTATGAGATGAGCCCAGACATTCCGCGTCGGGAACACCCAGCGTT
CCTGCATCCCATGCCATGA

Upstream 100 bases:

>100_bases
CCCACCGCCTCGCCATTTATGCCCGCCCCTTGCGAATGCTGCTCGGACTCGGCCGCTACGTCACCTCATTGCATGGCCGA
CACTATGATCGCAAGATGTG

Downstream 100 bases:

>100_bases
TCCTGTCCGTTCCCTCGGCTACAGTTCCCTCTACCCTGCCCCAAAGGAGATGTCGGTGACGTATCCCAGCCACGCCCGCA
CCACTGGACCGCTGTTAGCG

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MQQDPPTSQPHTPQIVDGVKPRGWVDHPAGLHFEYLGIADSRPTRTEYNECWAHQREVHAEVSAHQRPNTVIYVEHDPVY
TAGRRTRKEAYPFDGTPVVPVDRGGEITWHGPGQLVGYPIVFLQRGIGVVDYVRRVEEAVIRLVSQYGLRAGRVPGRTGV
WFPSDGMGPERKVCAIGIRVSRQTAMHGFALNIDPDTAGFDNIIPCGISDADVTSMARELRRLHGPDAEVPSLLEVAGNL
EPILTEMMSFQPYEMSPDIPRREHPAFLHPMP

Sequences:

>Translated_272_residues
MQQDPPTSQPHTPQIVDGVKPRGWVDHPAGLHFEYLGIADSRPTRTEYNECWAHQREVHAEVSAHQRPNTVIYVEHDPVY
TAGRRTRKEAYPFDGTPVVPVDRGGEITWHGPGQLVGYPIVFLQRGIGVVDYVRRVEEAVIRLVSQYGLRAGRVPGRTGV
WFPSDGMGPERKVCAIGIRVSRQTAMHGFALNIDPDTAGFDNIIPCGISDADVTSMARELRRLHGPDAEVPSLLEVAGNL
EPILTEMMSFQPYEMSPDIPRREHPAFLHPMP
>Mature_272_residues
MQQDPPTSQPHTPQIVDGVKPRGWVDHPAGLHFEYLGIADSRPTRTEYNECWAHQREVHAEVSAHQRPNTVIYVEHDPVY
TAGRRTRKEAYPFDGTPVVPVDRGGEITWHGPGQLVGYPIVFLQRGIGVVDYVRRVEEAVIRLVSQYGLRAGRVPGRTGV
WFPSDGMGPERKVCAIGIRVSRQTAMHGFALNIDPDTAGFDNIIPCGISDADVTSMARELRRLHGPDAEVPSLLEVAGNL
EPILTEMMSFQPYEMSPDIPRREHPAFLHPMP

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=153, Percent_Identity=39.8692810457516, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI87081767, Length=147, Percent_Identity=34.6938775510204, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323268, Length=216, Percent_Identity=26.8518518518519, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21355483, Length=173, Percent_Identity=38.728323699422, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_PROAC (Q6A9W7)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055407.1
- ProteinModelPortal:   Q6A9W7
- SMR:   Q6A9W7
- GeneID:   2932478
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0692
- NMPDR:   fig|267747.1.peg.684
- HOGENOM:   HBG716160
- OMA:   YNLAAER
- ProtClustDB:   CLSK2763458
- BioCyc:   PACN267747:PPA0692-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 30350; Mature: 30350

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 206-206

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQDPPTSQPHTPQIVDGVKPRGWVDHPAGLHFEYLGIADSRPTRTEYNECWAHQREVHA
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
EVSAHQRPNTVIYVEHDPVYTAGRRTRKEAYPFDGTPVVPVDRGGEITWHGPGQLVGYPI
HHHHCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCEEEECCCCCEEEECCCCCCHHHHH
VFLQRGIGVVDYVRRVEEAVIRLVSQYGLRAGRVPGRTGVWFPSDGMGPERKVCAIGIRV
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEE
SRQTAMHGFALNIDPDTAGFDNIIPCGISDADVTSMARELRRLHGPDAEVPSLLEVAGNL
CHHHHHCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
EPILTEMMSFQPYEMSPDIPRREHPAFLHPMP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQDPPTSQPHTPQIVDGVKPRGWVDHPAGLHFEYLGIADSRPTRTEYNECWAHQREVHA
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
EVSAHQRPNTVIYVEHDPVYTAGRRTRKEAYPFDGTPVVPVDRGGEITWHGPGQLVGYPI
HHHHCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCEEEECCCCCEEEECCCCCCHHHHH
VFLQRGIGVVDYVRRVEEAVIRLVSQYGLRAGRVPGRTGVWFPSDGMGPERKVCAIGIRV
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEE
SRQTAMHGFALNIDPDTAGFDNIIPCGISDADVTSMARELRRLHGPDAEVPSLLEVAGNL
CHHHHHCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
EPILTEMMSFQPYEMSPDIPRREHPAFLHPMP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA