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The map label for this gene is ptsM [H]

Identifier: 50842166

GI number: 50842166

Start: 745366

End: 745860

Strand: Direct

Name: ptsM [H]

Synonym: PPA0679

Alternate gene names: 50842166

Gene position: 745366-745860 (Clockwise)

Preceding gene: 50842165

Following gene: 50842167

Centisome position: 29.11

GC content: 63.23

Gene sequence:

>495_bases
ATGACCGCTATCGCATCTCCTCTCGACGGCACCCTGATGCCGTTGTCCGAGGTTCCTGATCCGGTCTTTGCGCAGGGTCT
CGTGGGGCCCGGTGTTGCCCTCGAACCCACGGGTGAGGGCGACGTCACTGTAGTGGCTCCGGTGGCAGGACGCATTGTCA
AGCTGCACCCGCACGCTTTCGTCATCCAGGCAGAGGGGTTCGGGGTACTCGTACACCTTGGCATTGACACCGTCCAGCTC
GATGGGGAGGGGTTCACCCTTCATGCTGAGGAGGGTCACGAGGTCGTCGTGGGAGACCCACTCATCACCTGGAATCCGGC
CGGGGTCGTTGCGGGTGGCCGCAGTGCGGTCTGCCCGATCGTCGTCCTCGACGCTTCCAGCGGAAGGATCGGTGACGCCG
CTGTTACCGGGGAGCGGATTGCCTGCGGCGATCAATTGTTCCAGTGGTTGGACGTTCCTGAGCAGGATGCCAATGTCTGT
GCCCAGCACGACTGA

Upstream 100 bases:

>100_bases
CTGCTGGTGCCTTCGGCGTTGTCCAGCAGGGTACGTCCGTTCAGGTCGTTGTTGGCCCCGAGGCCGACACTCTGGCCGAG
GACATTGAGGATCTGCGCTG

Downstream 100 bases:

>100_bases
TCGGGCCCGTCGTCACCCTGAGGTAGCGGCTCTGGTAGGGGTTGTCGCCGGAATTTTCTCGGCGATCGTCAAGTTTGGGT
GGGAAGTGCCTTTCCCACCG

Product: putative PTS system component IIA

Products: NA

Alternate protein names: EII-Man/EIII-Man; EIIBCA-Man; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component; Mannose permease IIC component; PTS system mannose-specific EIIC component; Mannose-specific phosphotransferase enzyme IIA component; PTS system mannose-specific EIIA component [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTAIASPLDGTLMPLSEVPDPVFAQGLVGPGVALEPTGEGDVTVVAPVAGRIVKLHPHAFVIQAEGFGVLVHLGIDTVQL
DGEGFTLHAEEGHEVVVGDPLITWNPAGVVAGGRSAVCPIVVLDASSGRIGDAAVTGERIACGDQLFQWLDVPEQDANVC
AQHD

Sequences:

>Translated_164_residues
MTAIASPLDGTLMPLSEVPDPVFAQGLVGPGVALEPTGEGDVTVVAPVAGRIVKLHPHAFVIQAEGFGVLVHLGIDTVQL
DGEGFTLHAEEGHEVVVGDPLITWNPAGVVAGGRSAVCPIVVLDASSGRIGDAAVTGERIACGDQLFQWLDVPEQDANVC
AQHD
>Mature_163_residues
TAIASPLDGTLMPLSEVPDPVFAQGLVGPGVALEPTGEGDVTVVAPVAGRIVKLHPHAFVIQAEGFGVLVHLGIDTVQLD
GEGFTLHAEEGHEVVVGDPLITWNPAGVVAGGRSAVCPIVVLDASSGRIGDAAVTGERIACGDQLFQWLDVPEQDANVCA
QHD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=122, Percent_Identity=43.4426229508197, Blast_Score=93, Evalue=9e-21,
Organism=Escherichia coli, GI1786894, Length=135, Percent_Identity=42.2222222222222, Blast_Score=88, Evalue=2e-19,
Organism=Escherichia coli, GI1790159, Length=126, Percent_Identity=39.6825396825397, Blast_Score=81, Evalue=4e-17,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 16827; Mature: 16696

Theoretical pI: Translated: 4.01; Mature: 4.01

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIASPLDGTLMPLSEVPDPVFAQGLVGPGVALEPTGEGDVTVVAPVAGRIVKLHPHAF
CCCCCCCCCCCEEEHHHCCCHHHHCCCCCCCEEECCCCCCCEEEEEECCCEEEEECCCEE
VIQAEGFGVLVHLGIDTVQLDGEGFTLHAEEGHEVVVGDPLITWNPAGVVAGGRSAVCPI
EEEECCCEEEEEECCEEEEECCCCEEEECCCCCEEEECCCEEEECCCCEEECCCCCCCEE
VVLDASSGRIGDAAVTGERIACGDQLFQWLDVPEQDANVCAQHD
EEEECCCCCCCCEEECCCEEECHHHHHHHHCCCCCCCCEEECCC
>Mature Secondary Structure 
TAIASPLDGTLMPLSEVPDPVFAQGLVGPGVALEPTGEGDVTVVAPVAGRIVKLHPHAF
CCCCCCCCCCEEEHHHCCCHHHHCCCCCCCEEECCCCCCCEEEEEECCCEEEEECCCEE
VIQAEGFGVLVHLGIDTVQLDGEGFTLHAEEGHEVVVGDPLITWNPAGVVAGGRSAVCPI
EEEECCCEEEEEECCEEEEECCCCEEEECCCCCEEEECCCEEEECCCCEEECCCCCCCEE
VVLDASSGRIGDAAVTGERIACGDQLFQWLDVPEQDANVCAQHD
EEEECCCCCCCCEEECCCEEECHHHHHHHHCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8039653; 12948626 [H]