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The map label for this gene is eno

Identifier: 50842029

GI number: 50842029

Start: 599061

End: 600341

Strand: Direct

Name: eno

Synonym: PPA0545

Alternate gene names: 50842029

Gene position: 599061-600341 (Clockwise)

Preceding gene: 50842028

Following gene: 50842030

Centisome position: 23.4

GC content: 60.5

Gene sequence:

>1281_bases
ATGGCAACCATCGAATTCATCGAAGCCCGTGAGATCCTCGATTCCCGCGGCAACCCGACCGTTGAGGTCGAGATGATCCT
CGACGACGGCACCCAGGCCCGCGCTGCGGTTCCTTCGGGCGCTTCGACCGGTCAGTTCGAGGCCGTTGAGCTGCGTGACG
GCGATAAGAAGCGTTACTCCGGTAAGGGTGTTCTCAAGGCTGTTGAGAACGTCAACGAGAAGATCGCCGAGGAGGTGCTC
GGCTGTGACGCGAGTGAGCAGCGCATTATCGACCAGATCATGATCGAGCTCGACGGATCCGACAACAAGGGCAAGTTGGG
TGCTAACGCCATCCTTGGTGTCTCCCTGGCTGCTGCTCATGCTGCTGCTGACTGTGCGGAGCTCCCGCTGTACCAGTACC
TCGGCGGACCGAACTCCCACGTGCTGCCCGTTCCAATGATGAACATCCTCAACGGTGGTGCCCACGCCGATTCCGACGTT
GACATCCAGGAGTTCATGATTGCCCCGATCGGCGCGGAATCCTTTAAGCAGGCCTACGAGTGGGGCGCTGCCGTTTACCA
CTCCCTCAAGAAGGTTCTCAAGGACAAGGGCTTGGCTACCGGTCTGGGTGACGAGGGCGGTTTCGCCCCCAACCTTCCCA
GCAACGCCGCCGCTTTGGACCTCATCCTTGACGCCATCAAGGCCGCCGGCTTCGAGCCGGGTAAGGACGTCGCCCTCGCC
CTTGATGTCGCTGCTTCCGAATTCTTCGAGGACGGCAAGTACACGTTCGAGGGCCAGGCTAAGACCTCGGCTGAGATGAT
CGCGTACTACGAAGGCCTCATCGCCAAGTATCCGCTGGTCTCTATTGAGGATCCGTTGGACGAGGAGGATTGGGACGGTT
GGGCCGAGTTCACGAAGAAGCTTGGTGAGAAGATCCAGATCGTTGGCGACGACTTGTTCGTCACTAACCCGAAGCGCCTT
GCCAAGGGAATCGAGACCAAGGCTGCCAACGCCCTGCTCGTCAAGGTGAACCAGATTGGCTCCCTCTCGGAGACTATCGA
CGCCGTCGAGCTGGCCCATCGCAACGGCTACCGCTGCATGATGTCGCACCGTTCTGGTGAGACCGAGGACACCACTATCG
CCGATCTCGCCGTCGCGCTATCGACTGGTCAGATTAAATCCGGTGCCCCGGCCCGCGGCGAGCGCATCGCCAAGTACAAC
CAGTTGCTGCGTATTGAGGAGGAACTGGGCGACTCGGCCGAGTACGCCGGTGCTTCCGCTTTCCCGCGCTTCCAGGCCTG
A

Upstream 100 bases:

>100_bases
TCAACGGCAGTGAGTGACGGTCAGCCCTGAGATACCTCTAGTTGGACGGCTCCGATACGCTTTGGGGTGGACGCAAAGAT
CCTCTTTGAAAGGTTGGTCC

Downstream 100 bases:

>100_bases
CTGACGCTATGAGGTGCTGACCGCAGCACCTGACATGACACGACCGTCCCGGTACTGCCGGGGCGGTCGTGTCATGATGG
ACCACGATGGCCACTACACC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MATIEFIEAREILDSRGNPTVEVEMILDDGTQARAAVPSGASTGQFEAVELRDGDKKRYSGKGVLKAVENVNEKIAEEVL
GCDASEQRIIDQIMIELDGSDNKGKLGANAILGVSLAAAHAAADCAELPLYQYLGGPNSHVLPVPMMNILNGGAHADSDV
DIQEFMIAPIGAESFKQAYEWGAAVYHSLKKVLKDKGLATGLGDEGGFAPNLPSNAAALDLILDAIKAAGFEPGKDVALA
LDVAASEFFEDGKYTFEGQAKTSAEMIAYYEGLIAKYPLVSIEDPLDEEDWDGWAEFTKKLGEKIQIVGDDLFVTNPKRL
AKGIETKAANALLVKVNQIGSLSETIDAVELAHRNGYRCMMSHRSGETEDTTIADLAVALSTGQIKSGAPARGERIAKYN
QLLRIEEELGDSAEYAGASAFPRFQA

Sequences:

>Translated_426_residues
MATIEFIEAREILDSRGNPTVEVEMILDDGTQARAAVPSGASTGQFEAVELRDGDKKRYSGKGVLKAVENVNEKIAEEVL
GCDASEQRIIDQIMIELDGSDNKGKLGANAILGVSLAAAHAAADCAELPLYQYLGGPNSHVLPVPMMNILNGGAHADSDV
DIQEFMIAPIGAESFKQAYEWGAAVYHSLKKVLKDKGLATGLGDEGGFAPNLPSNAAALDLILDAIKAAGFEPGKDVALA
LDVAASEFFEDGKYTFEGQAKTSAEMIAYYEGLIAKYPLVSIEDPLDEEDWDGWAEFTKKLGEKIQIVGDDLFVTNPKRL
AKGIETKAANALLVKVNQIGSLSETIDAVELAHRNGYRCMMSHRSGETEDTTIADLAVALSTGQIKSGAPARGERIAKYN
QLLRIEEELGDSAEYAGASAFPRFQA
>Mature_425_residues
ATIEFIEAREILDSRGNPTVEVEMILDDGTQARAAVPSGASTGQFEAVELRDGDKKRYSGKGVLKAVENVNEKIAEEVLG
CDASEQRIIDQIMIELDGSDNKGKLGANAILGVSLAAAHAAADCAELPLYQYLGGPNSHVLPVPMMNILNGGAHADSDVD
IQEFMIAPIGAESFKQAYEWGAAVYHSLKKVLKDKGLATGLGDEGGFAPNLPSNAAALDLILDAIKAAGFEPGKDVALAL
DVAASEFFEDGKYTFEGQAKTSAEMIAYYEGLIAKYPLVSIEDPLDEEDWDGWAEFTKKLGEKIQIVGDDLFVTNPKRLA
KGIETKAANALLVKVNQIGSLSETIDAVELAHRNGYRCMMSHRSGETEDTTIADLAVALSTGQIKSGAPARGERIAKYNQ
LLRIEEELGDSAEYAGASAFPRFQA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=56.8764568764569, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI301897477, Length=428, Percent_Identity=56.5420560747664, Blast_Score=447, Evalue=1e-126,
Organism=Homo sapiens, GI301897469, Length=428, Percent_Identity=56.5420560747664, Blast_Score=447, Evalue=1e-126,
Organism=Homo sapiens, GI4503571, Length=425, Percent_Identity=54.5882352941177, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=51.8779342723005, Blast_Score=390, Evalue=1e-109,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=27.4626865671642, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=27.4626865671642, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=27.4626865671642, Blast_Score=115, Evalue=9e-26,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=59.4339622641509, Blast_Score=485, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=56.8764568764569, Blast_Score=449, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=56.8764568764569, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=56.6137566137566, Blast_Score=216, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6321693, Length=428, Percent_Identity=55.1401869158878, Blast_Score=428, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6321968, Length=428, Percent_Identity=54.9065420560748, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=50.6944444444444, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=50.462962962963, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=50.462962962963, Blast_Score=398, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=54.6296296296296, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=54.6296296296296, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=54.6296296296296, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=54.6296296296296, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=54.6296296296296, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=54.6296296296296, Blast_Score=425, Evalue=1e-119,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_PROAC (Q6AAB8)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055256.1
- ProteinModelPortal:   Q6AAB8
- SMR:   Q6AAB8
- GeneID:   2932139
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0545
- NMPDR:   fig|267747.1.peg.533
- HOGENOM:   HBG726599
- OMA:   WTPRISS
- ProtClustDB:   PRK00077
- BioCyc:   PACN267747:PPA0545-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45531; Mature: 45399

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 335-335 BINDING 155-155 BINDING 164-164 BINDING 283-283 BINDING 310-310 BINDING 335-335 BINDING 386-386

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIEFIEAREILDSRGNPTVEVEMILDDGTQARAAVPSGASTGQFEAVELRDGDKKRYS
CCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCHHCC
GKGVLKAVENVNEKIAEEVLGCDASEQRIIDQIMIELDGSDNKGKLGANAILGVSLAAAH
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
AAADCAELPLYQYLGGPNSHVLPVPMMNILNGGAHADSDVDIQEFMIAPIGAESFKQAYE
HHHHHHHCCHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
WGAAVYHSLKKVLKDKGLATGLGDEGGFAPNLPSNAAALDLILDAIKAAGFEPGKDVALA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVAASEFFEDGKYTFEGQAKTSAEMIAYYEGLIAKYPLVSIEDPLDEEDWDGWAEFTKK
EHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHH
LGEKIQIVGDDLFVTNPKRLAKGIETKAANALLVKVNQIGSLSETIDAVELAHRNGYRCM
HCCEEEEEECCEEEECHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEE
MSHRSGETEDTTIADLAVALSTGQIKSGAPARGERIAKYNQLLRIEEELGDSAEYAGASA
EECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCC
FPRFQA
CCCCCC
>Mature Secondary Structure 
ATIEFIEAREILDSRGNPTVEVEMILDDGTQARAAVPSGASTGQFEAVELRDGDKKRYS
CCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCHHCC
GKGVLKAVENVNEKIAEEVLGCDASEQRIIDQIMIELDGSDNKGKLGANAILGVSLAAAH
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
AAADCAELPLYQYLGGPNSHVLPVPMMNILNGGAHADSDVDIQEFMIAPIGAESFKQAYE
HHHHHHHCCHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
WGAAVYHSLKKVLKDKGLATGLGDEGGFAPNLPSNAAALDLILDAIKAAGFEPGKDVALA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVAASEFFEDGKYTFEGQAKTSAEMIAYYEGLIAKYPLVSIEDPLDEEDWDGWAEFTKK
EHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHH
LGEKIQIVGDDLFVTNPKRLAKGIETKAANALLVKVNQIGSLSETIDAVELAHRNGYRCM
HCCEEEEEECCEEEECHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEE
MSHRSGETEDTTIADLAVALSTGQIKSGAPARGERIAKYNQLLRIEEELGDSAEYAGASA
EECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCC
FPRFQA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA