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The map label for this gene is folE [H]

Identifier: 50841751

GI number: 50841751

Start: 315622

End: 316197

Strand: Direct

Name: folE [H]

Synonym: PPA0261

Alternate gene names: 50841751

Gene position: 315622-316197 (Clockwise)

Preceding gene: 50841750

Following gene: 50841752

Centisome position: 12.33

GC content: 62.85

Gene sequence:

>576_bases
ATGAAATTGTCCGTTGATCGCGAGCGAGCCGCGGCAGCTATCCGCGAGTTTCTTCTGGCGGTCGGCGAGGATCCCGATCG
GGAAGGCCTGCGCGATACTCCAATGCGAGTGGCCCAGGCGGCCGAGGAGCTATTTGGCGGATTGAGCCAAGATCCGGGGG
AGTTGCTGGCAACGACCTTCGACATCTCCCACGACGAGATGGTGTTGGTGCGTGATATTGAGTTGCAGTCGATGTGTGAG
CACCATCTGCTGCCCTTCATCGGGGTAGCGCACGTCGGTTATATTCCGGCTAGGGACGGCCGGGTGACGGGGTTGAGTAA
GATCGCGAGGTTGGTGGAGGTCTATGCGCGTCGACCCCAGGTACAGGAGAGGTTGACGACCCAGGTGGCCGACGCCTTGG
TAGAACATTTGGACGCTCGTGGCGTGATCGTGGTTGTTGAGGCCGAGCACATGTGCATGACGACGAGGGGGGTCCGCAAG
CCGGGGGCCCGGACGGTGACCTCCGCGGTGCGCGGGATTCTGCGCAACCCAGCGACGCGGGCCGAGGCGATGGGGCTGAT
CACGGCTGGACGTTGA

Upstream 100 bases:

>100_bases
TCAAACGGTAACCCTTGGGGTCCGCCAAGGTCCTGATTATCAACGGCCTGCCGCACATATCGCGGCAGGCCGTCGTTCTA
GGCTGTCCGCTGTGAGTTCC

Downstream 100 bases:

>100_bases
CCATTTCAATGGGCCCTTACCTGGGCCCTGGGGCCTAACGTTTGAAGCTTTGACTTCCCTAGCATGGTAACAGTGTTCAG
TGCTGTGGGCGGACCGGCTG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MKLSVDRERAAAAIREFLLAVGEDPDREGLRDTPMRVAQAAEELFGGLSQDPGELLATTFDISHDEMVLVRDIELQSMCE
HHLLPFIGVAHVGYIPARDGRVTGLSKIARLVEVYARRPQVQERLTTQVADALVEHLDARGVIVVVEAEHMCMTTRGVRK
PGARTVTSAVRGILRNPATRAEAMGLITAGR

Sequences:

>Translated_191_residues
MKLSVDRERAAAAIREFLLAVGEDPDREGLRDTPMRVAQAAEELFGGLSQDPGELLATTFDISHDEMVLVRDIELQSMCE
HHLLPFIGVAHVGYIPARDGRVTGLSKIARLVEVYARRPQVQERLTTQVADALVEHLDARGVIVVVEAEHMCMTTRGVRK
PGARTVTSAVRGILRNPATRAEAMGLITAGR
>Mature_191_residues
MKLSVDRERAAAAIREFLLAVGEDPDREGLRDTPMRVAQAAEELFGGLSQDPGELLATTFDISHDEMVLVRDIELQSMCE
HHLLPFIGVAHVGYIPARDGRVTGLSKIARLVEVYARRPQVQERLTTQVADALVEHLDARGVIVVVEAEHMCMTTRGVRK
PGARTVTSAVRGILRNPATRAEAMGLITAGR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=179, Percent_Identity=51.3966480446927, Blast_Score=176, Evalue=8e-45,
Organism=Homo sapiens, GI4503949, Length=179, Percent_Identity=51.3966480446927, Blast_Score=176, Evalue=8e-45,
Organism=Homo sapiens, GI66932970, Length=149, Percent_Identity=48.993288590604, Blast_Score=141, Evalue=3e-34,
Organism=Homo sapiens, GI66932972, Length=139, Percent_Identity=51.7985611510791, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI1788476, Length=179, Percent_Identity=39.6648044692737, Blast_Score=104, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=50.561797752809, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6321706, Length=182, Percent_Identity=50.5494505494505, Blast_Score=176, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24656782, Length=181, Percent_Identity=48.0662983425414, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24656796, Length=181, Percent_Identity=48.0662983425414, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24656791, Length=181, Percent_Identity=48.0662983425414, Blast_Score=156, Evalue=7e-39,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 20914; Mature: 20914

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSVDRERAAAAIREFLLAVGEDPDREGLRDTPMRVAQAAEELFGGLSQDPGELLATTF
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHC
DISHDEMVLVRDIELQSMCEHHLLPFIGVAHVGYIPARDGRVTGLSKIARLVEVYARRPQ
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCH
VQERLTTQVADALVEHLDARGVIVVVEAEHMCMTTRGVRKPGARTVTSAVRGILRNPATR
HHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHH
AEAMGLITAGR
HHHHHHHCCCC
>Mature Secondary Structure
MKLSVDRERAAAAIREFLLAVGEDPDREGLRDTPMRVAQAAEELFGGLSQDPGELLATTF
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHC
DISHDEMVLVRDIELQSMCEHHLLPFIGVAHVGYIPARDGRVTGLSKIARLVEVYARRPQ
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCH
VQERLTTQVADALVEHLDARGVIVVVEAEHMCMTTRGVRKPGARTVTSAVRGILRNPATR
HHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHH
AEAMGLITAGR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]