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The map label for this gene is ywtE [H]

Identifier: 50841704

GI number: 50841704

Start: 263774

End: 264583

Strand: Direct

Name: ywtE [H]

Synonym: PPA0212

Alternate gene names: 50841704

Gene position: 263774-264583 (Clockwise)

Preceding gene: 50841703

Following gene: 50841705

Centisome position: 10.3

GC content: 62.96

Gene sequence:

>810_bases
GTGCTCATCGCAACTGACCTTGACGGAACCCTCCTGACTCCTGACGGCACTATCAGCCCTCGCACTCGTGATGCCATTCG
CGTCGCAGAGAAGGCCGGGGTGCCGGTGGTGCCAGTCACCGCACGGCAGATCTACGGTATTGAGAAGTGGCGTGACGATT
TAGGCCGCTGGGCACTGTGCTCCAACGGCGCCATTTGCTGGGACCTCCAAGCTCGCACCGTACTCTTTCGCCAACTCATG
CCGGGTGCCGTGGCGAACGAGTTCGCCCACCGTCTCCTCGATGCCGCTCCCGGCACCCGCTTTCTGACGATCAAGGACGA
CGGGCTCACTTTCGCCTCCCAGCGCGGCTATGCCGAATTGACGACGTTCGCTGACCATTCCCGCGAGCCTTCCGACATGC
CCGCCCTGGATCTCGACGAGGTGCTTGACGGAGACTGCCTCAAAATGGTCGCTCGCCATCCGAACCTGTCCGTCGAGGAA
CTCGCTGCGCGCGCGGCGAGCGTCGGTATGGCTGACGAGGTACACGTCACTACCTCGGGGAAGCCGATGCTAGAGATTAG
TGCTAAGGGGGTGGCCAAAGACTCCGGTTTGTCCATGCTGTGCGATCACCTGGGCATTGACCGGGCCGACACCGTGGCTT
TCGGTGACGGAGCTAATGACGTCGAGATGCTCGCCTGGGTGGGAGACTCCTACGCGATGGCTCATGCGGTGCCGTTGGCG
GTCGCAGCGGCCCGTCACCGGGCGCCGTCGAATGCCGAGGATGGGGTTGCGCAGGTCATCGAGAAGCTGCTGTCCATGAG
AGAACGGTGA

Upstream 100 bases:

>100_bases
GGAGGCGATTGCTGCCGCGGACAGGCGCGCTCCCTCCTGCGAAGAGGATGGTTTCGCCGTTGTTGTCGAGGAGTTGCTGG
CATCTCGATAATCTCGCAGT

Downstream 100 bases:

>100_bases
CGAACCATTCGGAGCTCAACATAGCGGTTGACAGGAAGTATATGAGGTACGGCCCTCTCGTAGGCGATCTTAATATAGGT
GTCGTTAGCGTCTTGACTAT

Product: putative hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MLIATDLDGTLLTPDGTISPRTRDAIRVAEKAGVPVVPVTARQIYGIEKWRDDLGRWALCSNGAICWDLQARTVLFRQLM
PGAVANEFAHRLLDAAPGTRFLTIKDDGLTFASQRGYAELTTFADHSREPSDMPALDLDEVLDGDCLKMVARHPNLSVEE
LAARAASVGMADEVHVTTSGKPMLEISAKGVAKDSGLSMLCDHLGIDRADTVAFGDGANDVEMLAWVGDSYAMAHAVPLA
VAAARHRAPSNAEDGVAQVIEKLLSMRER

Sequences:

>Translated_269_residues
MLIATDLDGTLLTPDGTISPRTRDAIRVAEKAGVPVVPVTARQIYGIEKWRDDLGRWALCSNGAICWDLQARTVLFRQLM
PGAVANEFAHRLLDAAPGTRFLTIKDDGLTFASQRGYAELTTFADHSREPSDMPALDLDEVLDGDCLKMVARHPNLSVEE
LAARAASVGMADEVHVTTSGKPMLEISAKGVAKDSGLSMLCDHLGIDRADTVAFGDGANDVEMLAWVGDSYAMAHAVPLA
VAAARHRAPSNAEDGVAQVIEKLLSMRER
>Mature_269_residues
MLIATDLDGTLLTPDGTISPRTRDAIRVAEKAGVPVVPVTARQIYGIEKWRDDLGRWALCSNGAICWDLQARTVLFRQLM
PGAVANEFAHRLLDAAPGTRFLTIKDDGLTFASQRGYAELTTFADHSREPSDMPALDLDEVLDGDCLKMVARHPNLSVEE
LAARAASVGMADEVHVTTSGKPMLEISAKGVAKDSGLSMLCDHLGIDRADTVAFGDGANDVEMLAWVGDSYAMAHAVPLA
VAAARHRAPSNAEDGVAQVIEKLLSMRER

Specific function: Unknown

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=273, Percent_Identity=28.2051282051282, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786982, Length=271, Percent_Identity=28.0442804428044, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI87081741, Length=249, Percent_Identity=25.7028112449799, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 28905; Mature: 28905

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIATDLDGTLLTPDGTISPRTRDAIRVAEKAGVPVVPVTARQIYGIEKWRDDLGRWALC
CEEEECCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCEEEE
SNGAICWDLQARTVLFRQLMPGAVANEFAHRLLDAAPGTRFLTIKDDGLTFASQRGYAEL
CCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEECCCCCCHHH
TTFADHSREPSDMPALDLDEVLDGDCLKMVARHPNLSVEELAARAASVGMADEVHVTTSG
HHHHHCCCCCCCCCCCCHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCC
KPMLEISAKGVAKDSGLSMLCDHLGIDRADTVAFGDGANDVEMLAWVGDSYAMAHAVPLA
CEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCHHHHHHHHHH
VAAARHRAPSNAEDGVAQVIEKLLSMRER
HHHHHHCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIATDLDGTLLTPDGTISPRTRDAIRVAEKAGVPVVPVTARQIYGIEKWRDDLGRWALC
CEEEECCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCEEEE
SNGAICWDLQARTVLFRQLMPGAVANEFAHRLLDAAPGTRFLTIKDDGLTFASQRGYAEL
CCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEECCCCCCHHH
TTFADHSREPSDMPALDLDEVLDGDCLKMVARHPNLSVEELAARAASVGMADEVHVTTSG
HHHHHCCCCCCCCCCCCHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCC
KPMLEISAKGVAKDSGLSMLCDHLGIDRADTVAFGDGANDVEMLAWVGDSYAMAHAVPLA
CEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCHHHHHHHHHH
VAAARHRAPSNAEDGVAQVIEKLLSMRER
HHHHHHCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]