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The map label for this gene is wecB [C]

Identifier: 50841637

GI number: 50841637

Start: 177696

End: 178769

Strand: Direct

Name: wecB [C]

Synonym: PPA0148

Alternate gene names: 50841637

Gene position: 177696-178769 (Clockwise)

Preceding gene: 50841636

Following gene: 50841638

Centisome position: 6.94

GC content: 64.25

Gene sequence:

>1074_bases
GTGCGCGTCGTGAGTATCGTCGGGGCTCGTCCCCAATTCGTGAAATTGGCCCCGGTGGCTAAGGCTTTCGCGGCCGAGGG
GATCGACCACAAGATCATCCATACCGGGCAGCATTACGACGTCAACATGTCGGATTCCTTCTTCGTCGAGCTCGGGATCC
CGAACCCGGACCTGCATCTTGGTGTAGGAGGCGGCTCCCACGGCGTCATGACCGGCCGGATGCTCGAGCAGCTTGATCCG
GTGCTTGAGGACATGGACCCAGACTGGGTGCTCGTTTACGGCGACACAAATTCGACCATTGCCGGCACCCTCTCGGCGGT
CAAGATGCACCTGCCGGTGGCCCACCTGGAAGCCGGGCTGCGTTCCTTCAACCGTCGCATGCCCGAGGAGCACAACCGAG
TTCTCACCGACCACGCCGCAGACCTGTGCCTGGCCCCGACCGAGGTAGCCATGGGACATCTGGCCGATGAGGGGCTGGCC
TCCCGTGCAGTTCTTGTCGGAGACGTCATGACCGACGTGTGCCTGCAGGTGCGCGATTCCGTCCTCGCCAACCCGCGCCC
GGTACCCGGTGTCGAGGCGGGCGAGGAGTACGTCATGGCGACGATCCACCGTGCCGACAACACTGACGACCCCAAGCGTC
TGGCCCACATTCTTGACGCCCTCGGCAGCCTAGATCGCAAGGTCATCCTGCCGGTACACCCGCGCCTGCGCGCCCGCGCC
GAGTCCGACGGCTTTACTGTCGCCCGCGGCAACCTCACCACCATTGACCCGTTGGCTTACCCCGACATGGTTAACGCCGT
CACCAACGCCGCTGGCGTCATCACCGACTCGGGTGGTTTGCAAAAAGAAGCCTTCCTGCTTGGCACTATCTGCACGACGG
TGCGCACCGAGACCGAGTGGGTGGAGACCGTCGAGAACGGTTGGAACGTGCTTGATCCCGATGCCGAGCACCTGGGCGAG
TACGCCACGCGCCCGCACCCCGAGGGCGATCCGGGGTATCCCTACGGTGACGGCAAGGCGGCTCGCAATGTCGCCACCGT
GTTGTTGAAAACCGGCGAGCGAGACCGTTCCTGA

Upstream 100 bases:

>100_bases
TAGTGGAAGGTAGAGTGCCAGCATTGCGCCCAGTTCCCGATTCGGGCGCTGCCATGTGGCCTCGGGGAGAGGCTGACTAT
CTCATCGAGAGGATTGCGTC

Downstream 100 bases:

>100_bases
TGGCGAATTCTTCCCGCCTCGGCAGGGTCGTCTCCCTTTCGACAGTTCATCACGGTCACGACAACCGGGTTTTCAACAAA
GAAGCCAAGGCTCTTGTCGC

Product: putative UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MRVVSIVGARPQFVKLAPVAKAFAAEGIDHKIIHTGQHYDVNMSDSFFVELGIPNPDLHLGVGGGSHGVMTGRMLEQLDP
VLEDMDPDWVLVYGDTNSTIAGTLSAVKMHLPVAHLEAGLRSFNRRMPEEHNRVLTDHAADLCLAPTEVAMGHLADEGLA
SRAVLVGDVMTDVCLQVRDSVLANPRPVPGVEAGEEYVMATIHRADNTDDPKRLAHILDALGSLDRKVILPVHPRLRARA
ESDGFTVARGNLTTIDPLAYPDMVNAVTNAAGVITDSGGLQKEAFLLGTICTTVRTETEWVETVENGWNVLDPDAEHLGE
YATRPHPEGDPGYPYGDGKAARNVATVLLKTGERDRS

Sequences:

>Translated_357_residues
MRVVSIVGARPQFVKLAPVAKAFAAEGIDHKIIHTGQHYDVNMSDSFFVELGIPNPDLHLGVGGGSHGVMTGRMLEQLDP
VLEDMDPDWVLVYGDTNSTIAGTLSAVKMHLPVAHLEAGLRSFNRRMPEEHNRVLTDHAADLCLAPTEVAMGHLADEGLA
SRAVLVGDVMTDVCLQVRDSVLANPRPVPGVEAGEEYVMATIHRADNTDDPKRLAHILDALGSLDRKVILPVHPRLRARA
ESDGFTVARGNLTTIDPLAYPDMVNAVTNAAGVITDSGGLQKEAFLLGTICTTVRTETEWVETVENGWNVLDPDAEHLGE
YATRPHPEGDPGYPYGDGKAARNVATVLLKTGERDRS
>Mature_357_residues
MRVVSIVGARPQFVKLAPVAKAFAAEGIDHKIIHTGQHYDVNMSDSFFVELGIPNPDLHLGVGGGSHGVMTGRMLEQLDP
VLEDMDPDWVLVYGDTNSTIAGTLSAVKMHLPVAHLEAGLRSFNRRMPEEHNRVLTDHAADLCLAPTEVAMGHLADEGLA
SRAVLVGDVMTDVCLQVRDSVLANPRPVPGVEAGEEYVMATIHRADNTDDPKRLAHILDALGSLDRKVILPVHPRLRARA
ESDGFTVARGNLTTIDPLAYPDMVNAVTNAAGVITDSGGLQKEAFLLGTICTTVRTETEWVETVENGWNVLDPDAEHLGE
YATRPHPEGDPGYPYGDGKAARNVATVLLKTGERDRS

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=373, Percent_Identity=31.6353887399464, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 38517; Mature: 38517

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVSIVGARPQFVKLAPVAKAFAAEGIDHKIIHTGQHYDVNMSDSFFVELGIPNPDLHL
CEEEEEECCCCCEEEHHHHHHHHHHCCCCCEEEECCCCEEECCCCCEEEEECCCCCCEEE
GVGGGSHGVMTGRMLEQLDPVLEDMDPDWVLVYGDTNSTIAGTLSAVKMHLPVAHLEAGL
EECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHH
RSFNRRMPEEHNRVLTDHAADLCLAPTEVAMGHLADEGLASRAVLVGDVMTDVCLQVRDS
HHHHHCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VLANPRPVPGVEAGEEYVMATIHRADNTDDPKRLAHILDALGSLDRKVILPVHPRLRARA
HHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCC
ESDGFTVARGNLTTIDPLAYPDMVNAVTNAAGVITDSGGLQKEAFLLGTICTTVRTETEW
CCCCEEEECCCCCEECCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCHHHH
VETVENGWNVLDPDAEHLGEYATRPHPEGDPGYPYGDGKAARNVATVLLKTGERDRS
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRVVSIVGARPQFVKLAPVAKAFAAEGIDHKIIHTGQHYDVNMSDSFFVELGIPNPDLHL
CEEEEEECCCCCEEEHHHHHHHHHHCCCCCEEEECCCCEEECCCCCEEEEECCCCCCEEE
GVGGGSHGVMTGRMLEQLDPVLEDMDPDWVLVYGDTNSTIAGTLSAVKMHLPVAHLEAGL
EECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHH
RSFNRRMPEEHNRVLTDHAADLCLAPTEVAMGHLADEGLASRAVLVGDVMTDVCLQVRDS
HHHHHCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VLANPRPVPGVEAGEEYVMATIHRADNTDDPKRLAHILDALGSLDRKVILPVHPRLRARA
HHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCC
ESDGFTVARGNLTTIDPLAYPDMVNAVTNAAGVITDSGGLQKEAFLLGTICTTVRTETEW
CCCCEEEECCCCCEECCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCHHHH
VETVENGWNVLDPDAEHLGEYATRPHPEGDPGYPYGDGKAARNVATVLLKTGERDRS
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA