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The map label for this gene is yfiJ [H]

Identifier: 50841559

GI number: 50841559

Start: 70321

End: 71691

Strand: Reverse

Name: yfiJ [H]

Synonym: PPA0068

Alternate gene names: 50841559

Gene position: 71691-70321 (Counterclockwise)

Preceding gene: 50841561

Following gene: 50841557

Centisome position: 2.8

GC content: 63.97

Gene sequence:

>1371_bases
ATGGAGGACACCGGATCGCAGACCTTGCCCACGGAGAACCGTTCCACCGGCTCCCGCGGGGCGGGGGTACGCGCCCGTCT
CAATCGGTTCAATCCGAGCCGCAATAACGTTCCCCAAATCAACATCCCCGGTATGGAGGAGGTGCTGGACCCCAAGCTCG
ATGACGGCCGCCCGGCTGACACCGAACGGGCATGGTGGGGAGACGTCACCCTGACCCTCATCATGGCCGTAGTGACGATC
GCCCCCTACCTCGTCAGTCTGGTGGAGTGGCCGCGCCCGGAGTACATCGCCGCCCTCTTGTCATCGATGGTCATGGTGGC
CTCTCTGCCGATGCGTCGCGAGCATCCTCTTCTCATGATGACCTTGGTCGCTATCGGGGCTGCGATCCAGTTCTTCTTCG
TGCCTTTCCCGGTGTTGAGCATCTTCGTCGTCCCGATCGCCTCCTACTCGGTGGCGAGGTGGGTCGACGGCCACCAATCC
CGCTGGGTGCTGTGGGTGGGAGCCGTGGGGTCAGTCATTGGACCGATGAGGTGGATCCCCACCATTGCCGCCGGTTACGA
TCCCTCATCGGGAACGCCCTGGGTGCTACTGGTGCTGGCCATGACGGTGTGTGCGGGCCTCGTCCTCACCCCATACGCGG
TAGGTCGACGACTGCGGGAGTCCGCCCTCATCGACTCCCAGCAACGCATCGTCAAAGCCCAGCGGTACCGGGCGATCCTC
GCCGAACGTGAACAGCAGGCCAGGGTGGCCGAGGAGCGCACCCGTAACGAGATCGCCAGAGAGCTCCACGACATCGTCGC
CCACTCGCTGTCGGTCATGATTGTGCAGGCCGAAGGCGGCAAAGCACTGGCGACGAAGAAGCCGGAGAAGGCCCCCGAGG
TGCTTGAGACGATCGCCGAGACCGGACGTGAGGCCCTCCTTGAAATGAGGCGAATCGTCGGGGTGTTGCGTCAGGACACC
GACGCCGAGTTCTCCCCCTCCCCTAGCCTGGCTGAAATTCCCGACCTGGTGGACAAGTCCGGGGTGAAAGTTGAACTCAA
GGTGACTGGGGAGGAGCCGGTGGTCTCCCAGGCTCTTGGGCTTGTGATCTACCGAGTCGTTCAGGAGGCCCTCACCAACG
TCCTCAAACACGCTGGCCCGAGCGCCCACGCCACTGTGACCCTGGCCTATTCACCTACCGCGATCTACGTGGAGATTCTC
GACGACGGGTACGGGGCTGCCGTCCAAGGGGACGGTCGGGGACACGGTCTCCAGGGGATGCGAGAGCGCGTTACGTCAAT
GGGTGGGCAATTGTCGGCGGGGCCTCGCAAAGAGGGCGGGTTCCGCGTCATGGCGAAACTGCCGGTCCAAGCCGACTCCC
GTCAGAGATGA

Upstream 100 bases:

>100_bases
GTGTGGTGAATTGGTCACGAACGGTGCGCGTTGCCAGCCCACGCGTCGCTAACTGGCGTGCCCAACCAATCTTCCACAGC
TGGTTTCTAGAATTGCTGTT

Downstream 100 bases:

>100_bases
CCTCGGCTCCGACGTCACTTACCCCAACAGTTCGGGGAGGAACCCTAGCCGCTCCGGGGCCCGCGGTACCCAGGCGAGCA
CATCCTCAGGTGTGAGTGGT

Product: putative two component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MEDTGSQTLPTENRSTGSRGAGVRARLNRFNPSRNNVPQINIPGMEEVLDPKLDDGRPADTERAWWGDVTLTLIMAVVTI
APYLVSLVEWPRPEYIAALLSSMVMVASLPMRREHPLLMMTLVAIGAAIQFFFVPFPVLSIFVVPIASYSVARWVDGHQS
RWVLWVGAVGSVIGPMRWIPTIAAGYDPSSGTPWVLLVLAMTVCAGLVLTPYAVGRRLRESALIDSQQRIVKAQRYRAIL
AEREQQARVAEERTRNEIARELHDIVAHSLSVMIVQAEGGKALATKKPEKAPEVLETIAETGREALLEMRRIVGVLRQDT
DAEFSPSPSLAEIPDLVDKSGVKVELKVTGEEPVVSQALGLVIYRVVQEALTNVLKHAGPSAHATVTLAYSPTAIYVEIL
DDGYGAAVQGDGRGHGLQGMRERVTSMGGQLSAGPRKEGGFRVMAKLPVQADSRQR

Sequences:

>Translated_456_residues
MEDTGSQTLPTENRSTGSRGAGVRARLNRFNPSRNNVPQINIPGMEEVLDPKLDDGRPADTERAWWGDVTLTLIMAVVTI
APYLVSLVEWPRPEYIAALLSSMVMVASLPMRREHPLLMMTLVAIGAAIQFFFVPFPVLSIFVVPIASYSVARWVDGHQS
RWVLWVGAVGSVIGPMRWIPTIAAGYDPSSGTPWVLLVLAMTVCAGLVLTPYAVGRRLRESALIDSQQRIVKAQRYRAIL
AEREQQARVAEERTRNEIARELHDIVAHSLSVMIVQAEGGKALATKKPEKAPEVLETIAETGREALLEMRRIVGVLRQDT
DAEFSPSPSLAEIPDLVDKSGVKVELKVTGEEPVVSQALGLVIYRVVQEALTNVLKHAGPSAHATVTLAYSPTAIYVEIL
DDGYGAAVQGDGRGHGLQGMRERVTSMGGQLSAGPRKEGGFRVMAKLPVQADSRQR
>Mature_456_residues
MEDTGSQTLPTENRSTGSRGAGVRARLNRFNPSRNNVPQINIPGMEEVLDPKLDDGRPADTERAWWGDVTLTLIMAVVTI
APYLVSLVEWPRPEYIAALLSSMVMVASLPMRREHPLLMMTLVAIGAAIQFFFVPFPVLSIFVVPIASYSVARWVDGHQS
RWVLWVGAVGSVIGPMRWIPTIAAGYDPSSGTPWVLLVLAMTVCAGLVLTPYAVGRRLRESALIDSQQRIVKAQRYRAIL
AEREQQARVAEERTRNEIARELHDIVAHSLSVMIVQAEGGKALATKKPEKAPEVLETIAETGREALLEMRRIVGVLRQDT
DAEFSPSPSLAEIPDLVDKSGVKVELKVTGEEPVVSQALGLVIYRVVQEALTNVLKHAGPSAHATVTLAYSPTAIYVEIL
DDGYGAAVQGDGRGHGLQGMRERVTSMGGQLSAGPRKEGGFRVMAKLPVQADSRQR

Specific function: Member of the two-component regulatory system yfiJ/yfiK. Probably activates yfiK by phosphorylation [H]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=248, Percent_Identity=29.4354838709677, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082311, Length=235, Percent_Identity=29.3617021276596, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788812, Length=198, Percent_Identity=27.2727272727273, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011712 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49731; Mature: 49731

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure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HHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]