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The map label for this gene is chbF [C]

Identifier: 50841552

GI number: 50841552

Start: 60248

End: 61636

Strand: Direct

Name: chbF [C]

Synonym: PPA0061

Alternate gene names: 50841552

Gene position: 60248-61636 (Clockwise)

Preceding gene: 50841551

Following gene: 50841553

Centisome position: 2.35

GC content: 63.64

Gene sequence:

>1389_bases
ATGAAGCTCACCATCCTCGGTGGCGGAGGATTCCGCGTTCCGCTGGTATTTAAGGCCCTTGCTCACGACACCTCGCCGCA
ACGAGTCACCGAACTGCGGCTCTACGACACCGACCCGCTCCGGCTAGGCGTCATCGAGACGGTGGTAGCCCAGCTGGCCC
GCGCCCTGCCTCACGCGCCGTCAGTGGTAGCTACCACCGACCTGCCCACCGCTTTGGCGGGAACCGACTTCATCTTTTCG
GCCATCCGCGTCGCCGGCACCCATGGACGTGCCCTCGATGAGTCGATGTGCCTGGCTCGCGGGGTCATCGGTCAAGAGAC
CGTAGGAGCTGGCGGTATTTCGTATGCTTTGCGGGGGATCCCGGTGGTACTCGACCTCGTCGAGCAGATCACGCGGTATG
CCCCCGACGCCAAGGTCATCAACTTCACTAACCCGGCGGGGGTGATGACTGAGGTCATGCAGCGTCGCCTGGGAGACCAG
GTAGTGGGCATCTGCGACTCTCCAGTGGGTCTGGCCCGTCGCATCCTCACCACCCTGCAGCGTGCCGGGCTGGCACCGGC
CGACCTGGGGTCCCTATTCGACGGCGACGGGCGCATTCACATCGGCTACAGCGGACTCAACCACTTAGGTTGGCTTACCA
GCCTAAACGTTGATGGGGTCGACGTCCTGCCGCGTCTGCTTGAGCGACCCGACCTCATTGAGAGCTTCGAGGAAGGTCGT
CTCTTCGGTGCCGACCTGGTGCAAGCCTTGGGCGCCGTGCCTAACGAATACCTGCATTACTACTACTATTCCCGAGACGA
CCTCGCCGCCGATCGGAAGGCTGAAGCCCCGCGCGGAGCCTTCCTGGAGGCCCAGCAGAACCAGTTCTACACCCAGGCCC
AACACACTGACGATGTCGCTGGCCTATGGGAAGCCACCCGTCTGGAGCGAGAGGAGACCTACATGGCCACCAATCGCGAG
GTCTCGGGTGAATTCGAGCGCGATGAAGCCGACTTAGAGACCGGCGGATACGACAAGGTTGCGCTGGCGATCATGCACGC
CATCGCCAACGACGTACCCGCCGAGCTCATCCTCAACGTCGCCAACCACGGGGCCCTTCCCGACCTGCCCGACGAGGCCG
TCGTGGAGGTCCCGTGCCGGGTAGACGGTGCCGGGATTCACCGGCTGCCCACTCCCGCGCTGCCCGACCATGCCCGAGGA
CTTGTCGTTAACGCCAAGTACGTAGAACAACGCACCATCGACGCTGCTGTTAACCACAGCCGTACCGCCGCCCTGCTGGC
CCTGAGCCACCACCCGCTTGTTGACTCCGTTCACGTCGCCGAACAGCTGCTTGACGACTTCGCTGACGCCTTCGACGGCC
TGGAGTACCTCAAGGACGCCCATGCATGA

Upstream 100 bases:

>100_bases
CCCCGTTCACCCTCGAGGAGGTCAGTGGCGTCATTACCACCGGGCCGCTGCCACCGGACTTCGACGCACACTGCGAATCA
ACAGTGGAGGTCATTACCGC

Downstream 100 bases:

>100_bases
TCACACCGAACTCATCCAGGACCGGATTGGCCATCTCAAGGAACGTCTCGAACGAGAAATCACCGAGAAGATCTGTCCAT
TGCAGGTGACGGCCTGGCAG

Product: putative glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKLTILGGGGFRVPLVFKALAHDTSPQRVTELRLYDTDPLRLGVIETVVAQLARALPHAPSVVATTDLPTALAGTDFIFS
AIRVAGTHGRALDESMCLARGVIGQETVGAGGISYALRGIPVVLDLVEQITRYAPDAKVINFTNPAGVMTEVMQRRLGDQ
VVGICDSPVGLARRILTTLQRAGLAPADLGSLFDGDGRIHIGYSGLNHLGWLTSLNVDGVDVLPRLLERPDLIESFEEGR
LFGADLVQALGAVPNEYLHYYYYSRDDLAADRKAEAPRGAFLEAQQNQFYTQAQHTDDVAGLWEATRLEREETYMATNRE
VSGEFERDEADLETGGYDKVALAIMHAIANDVPAELILNVANHGALPDLPDEAVVEVPCRVDGAGIHRLPTPALPDHARG
LVVNAKYVEQRTIDAAVNHSRTAALLALSHHPLVDSVHVAEQLLDDFADAFDGLEYLKDAHA

Sequences:

>Translated_462_residues
MKLTILGGGGFRVPLVFKALAHDTSPQRVTELRLYDTDPLRLGVIETVVAQLARALPHAPSVVATTDLPTALAGTDFIFS
AIRVAGTHGRALDESMCLARGVIGQETVGAGGISYALRGIPVVLDLVEQITRYAPDAKVINFTNPAGVMTEVMQRRLGDQ
VVGICDSPVGLARRILTTLQRAGLAPADLGSLFDGDGRIHIGYSGLNHLGWLTSLNVDGVDVLPRLLERPDLIESFEEGR
LFGADLVQALGAVPNEYLHYYYYSRDDLAADRKAEAPRGAFLEAQQNQFYTQAQHTDDVAGLWEATRLEREETYMATNRE
VSGEFERDEADLETGGYDKVALAIMHAIANDVPAELILNVANHGALPDLPDEAVVEVPCRVDGAGIHRLPTPALPDHARG
LVVNAKYVEQRTIDAAVNHSRTAALLALSHHPLVDSVHVAEQLLDDFADAFDGLEYLKDAHA
>Mature_462_residues
MKLTILGGGGFRVPLVFKALAHDTSPQRVTELRLYDTDPLRLGVIETVVAQLARALPHAPSVVATTDLPTALAGTDFIFS
AIRVAGTHGRALDESMCLARGVIGQETVGAGGISYALRGIPVVLDLVEQITRYAPDAKVINFTNPAGVMTEVMQRRLGDQ
VVGICDSPVGLARRILTTLQRAGLAPADLGSLFDGDGRIHIGYSGLNHLGWLTSLNVDGVDVLPRLLERPDLIESFEEGR
LFGADLVQALGAVPNEYLHYYYYSRDDLAADRKAEAPRGAFLEAQQNQFYTQAQHTDDVAGLWEATRLEREETYMATNRE
VSGEFERDEADLETGGYDKVALAIMHAIANDVPAELILNVANHGALPDLPDEAVVEVPCRVDGAGIHRLPTPALPDHARG
LVVNAKYVEQRTIDAAVNHSRTAALLALSHHPLVDSVHVAEQLLDDFADAFDGLEYLKDAHA

Specific function: Hydrolyzes A Wide Variety Of P-Beta-Glucosides Including Cellobiose-6p, Salicin-6p, Arbutin-6p, Gentiobiose-6p, Methyl- Beta-Glucoside-6p And P-Nitrophenyl-Beta-D-Glucopyranoside-6p. Is Also Able To Hydrolyze Phospho-N,N'-Diacetylchitobiose. [C]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=491, Percent_Identity=28.1059063136456, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1790560, Length=449, Percent_Identity=24.9443207126949, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.122 [H]

Molecular weight: Translated: 49964; Mature: 49964

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTILGGGGFRVPLVFKALAHDTSPQRVTELRLYDTDPLRLGVIETVVAQLARALPHAP
CEEEEECCCCCCHHHHHHHHHCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCC
SVVATTDLPTALAGTDFIFSAIRVAGTHGRALDESMCLARGVIGQETVGAGGISYALRGI
CEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
PVVLDLVEQITRYAPDAKVINFTNPAGVMTEVMQRRLGDQVVGICDSPVGLARRILTTLQ
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHH
RAGLAPADLGSLFDGDGRIHIGYSGLNHLGWLTSLNVDGVDVLPRLLERPDLIESFEEGR
HCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHEECCCCCHHHHHHHHCCCHHHHHHHCCC
LFGADLVQALGAVPNEYLHYYYYSRDDLAADRKAEAPRGAFLEAQQNQFYTQAQHTDDVA
EEHHHHHHHHHCCCCHHEEEEEECCCCCCCCCCCCCCCCCCEECHHHHHHHHHCCCCHHH
GLWEATRLEREETYMATNREVSGEFERDEADLETGGYDKVALAIMHAIANDVPAELILNV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
ANHGALPDLPDEAVVEVPCRVDGAGIHRLPTPALPDHARGLVVNAKYVEQRTIDAAVNHS
HHCCCCCCCCCHHHEEECCEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCC
RTAALLALSHHPLVDSVHVAEQLLDDFADAFDGLEYLKDAHA
HHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLTILGGGGFRVPLVFKALAHDTSPQRVTELRLYDTDPLRLGVIETVVAQLARALPHAP
CEEEEECCCCCCHHHHHHHHHCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCC
SVVATTDLPTALAGTDFIFSAIRVAGTHGRALDESMCLARGVIGQETVGAGGISYALRGI
CEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
PVVLDLVEQITRYAPDAKVINFTNPAGVMTEVMQRRLGDQVVGICDSPVGLARRILTTLQ
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHH
RAGLAPADLGSLFDGDGRIHIGYSGLNHLGWLTSLNVDGVDVLPRLLERPDLIESFEEGR
HCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHEECCCCCHHHHHHHHCCCHHHHHHHCCC
LFGADLVQALGAVPNEYLHYYYYSRDDLAADRKAEAPRGAFLEAQQNQFYTQAQHTDDVA
EEHHHHHHHHHCCCCHHEEEEEECCCCCCCCCCCCCCCCCCEECHHHHHHHHHCCCCHHH
GLWEATRLEREETYMATNREVSGEFERDEADLETGGYDKVALAIMHAIANDVPAELILNV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
ANHGALPDLPDEAVVEVPCRVDGAGIHRLPTPALPDHARGLVVNAKYVEQRTIDAAVNHS
HHCCCCCCCCCHHHEEECCEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCC
RTAALLALSHHPLVDSVHVAEQLLDDFADAFDGLEYLKDAHA
HHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA