Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 50365490

GI number: 50365490

Start: 785698

End: 786441

Strand: Reverse

Name: holB [H]

Synonym: Mfl675

Alternate gene names: 50365490

Gene position: 786441-785698 (Counterclockwise)

Preceding gene: 50365491

Following gene: 50365489

Centisome position: 99.14

GC content: 23.66

Gene sequence:

>744_bases
ATGAAAAAAAGAGAAGCGTTAGAAAAATTTGTTGAGCAATTAAAAAATAAAAAGATGTTTAGCTCTATATTAATTTCAAA
CGATAATCAAGAAACTTTAAATGATTTTGCAAACAATTTATCTAGAACAATTTTTTGTGAAAACTTAGGTATTGAAAAAG
ATTCTTGTGATAACTGCAAAAGATTTGAAAATAAAAGTTTGTCAAATTTTGTAGTATTAGGAGATGGAAGTCTTGCAATA
AACAAAAATGACGTCGTTGAACTTATGCAAAAGTTTTCTTTGACGACTAATGAAGTTAATAAGTTTAAAGTATATGTTTT
ATCTAATGCAGAAAATTTAAAAAATGAATCTGCAAACTCTTTATTAAAATTTATTGAAGAACCCCCTGAAAATACAATTG
CTATTTTATTAACTAAAAATAAAAGTCAAGTTTTACCAACCATAAAATCAAGATGTAAATTAATTGTTTTAGAAAATGAA
AATCAAGATGAATCACCTAAAAATATTATTGAAGAACTTTTAGAAAAAGATAAAAACTCAATTTTGCTTGCAAACGCTGA
ATTGAAAAAGATTGATAAGTCTGAATTAATTGCTATGCTAGAAGTTGCATACAATAGAACAATAATAAAAAAATATTTAA
ATTTAGCAGAAGCAACTTTGCAATTAATTAATGATTTAAAATTTACATTTCAAACAAATTTAGCAATAGATGTTTTCTTA
ATACATATAAGTGAGGTAGTGTAA

Upstream 100 bases:

>100_bases
TGGTTAAAGAACCAAATAGAATAACTGTCATTGATGCTTCAAAATCACCTGAAGAAGTTTTTGAAGAAACTAAAAAAGTT
ATCTTAGAAAAGATTGAATT

Downstream 100 bases:

>100_bases
TGAAAATTTTAAATGACCTTTTAGGTTATGAAGATAGAAAAATTTATCAAGACTCGGAAATGTTTAACTTCACATTAGAT
AGTGTATTGATTGCAAGATT

Product: DNA polymerase III delta' subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKKREALEKFVEQLKNKKMFSSILISNDNQETLNDFANNLSRTIFCENLGIEKDSCDNCKRFENKSLSNFVVLGDGSLAI
NKNDVVELMQKFSLTTNEVNKFKVYVLSNAENLKNESANSLLKFIEEPPENTIAILLTKNKSQVLPTIKSRCKLIVLENE
NQDESPKNIIEELLEKDKNSILLANAELKKIDKSELIAMLEVAYNRTIIKKYLNLAEATLQLINDLKFTFQTNLAIDVFL
IHISEVV

Sequences:

>Translated_247_residues
MKKREALEKFVEQLKNKKMFSSILISNDNQETLNDFANNLSRTIFCENLGIEKDSCDNCKRFENKSLSNFVVLGDGSLAI
NKNDVVELMQKFSLTTNEVNKFKVYVLSNAENLKNESANSLLKFIEEPPENTIAILLTKNKSQVLPTIKSRCKLIVLENE
NQDESPKNIIEELLEKDKNSILLANAELKKIDKSELIAMLEVAYNRTIIKKYLNLAEATLQLINDLKFTFQTNLAIDVFL
IHISEVV
>Mature_247_residues
MKKREALEKFVEQLKNKKMFSSILISNDNQETLNDFANNLSRTIFCENLGIEKDSCDNCKRFENKSLSNFVVLGDGSLAI
NKNDVVELMQKFSLTTNEVNKFKVYVLSNAENLKNESANSLLKFIEEPPENTIAILLTKNKSQVLPTIKSRCKLIVLENE
NQDESPKNIIEELLEKDKNSILLANAELKKIDKSELIAMLEVAYNRTIIKKYLNLAEATLQLINDLKFTFQTNLAIDVFL
IHISEVV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 28193; Mature: 28193

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKREALEKFVEQLKNKKMFSSILISNDNQETLNDFANNLSRTIFCENLGIEKDSCDNCK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCHHHH
RFENKSLSNFVVLGDGSLAINKNDVVELMQKFSLTTNEVNKFKVYVLSNAENLKNESANS
HHCCCCCCCEEEEECCEEEECHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHCCHHHHH
LLKFIEEPPENTIAILLTKNKSQVLPTIKSRCKLIVLENENQDESPKNIIEELLEKDKNS
HHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC
ILLANAELKKIDKSELIAMLEVAYNRTIIKKYLNLAEATLQLINDLKFTFQTNLAIDVFL
EEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHH
IHISEVV
HHHHHCC
>Mature Secondary Structure
MKKREALEKFVEQLKNKKMFSSILISNDNQETLNDFANNLSRTIFCENLGIEKDSCDNCK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCHHHH
RFENKSLSNFVVLGDGSLAINKNDVVELMQKFSLTTNEVNKFKVYVLSNAENLKNESANS
HHCCCCCCCEEEEECCEEEECHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHCCHHHHH
LLKFIEEPPENTIAILLTKNKSQVLPTIKSRCKLIVLENENQDESPKNIIEELLEKDKNS
HHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC
ILLANAELKKIDKSELIAMLEVAYNRTIIKKYLNLAEATLQLINDLKFTFQTNLAIDVFL
EEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHH
IHISEVV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]