Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 50365488

GI number: 50365488

Start: 783776

End: 784972

Strand: Reverse

Name: tilS [H]

Synonym: Mfl673

Alternate gene names: 50365488

Gene position: 784972-783776 (Counterclockwise)

Preceding gene: 50365489

Following gene: 50365487

Centisome position: 98.96

GC content: 20.63

Gene sequence:

>1197_bases
ATGGAAAGATTTAAATTAAATACAACACTTTCATATCTAGTTGCTGTGTCAGGTGGGCCTGATAGTATGTTTATGTTAAA
CGAATTAATTAAAATGAATATTAAAGAAATTGTTGTTTGTCATGTTAATTATAATTTCAGAGAAGACTCATGAAAAGATC
AAAAACTAGTTGAAGATTTCTGTTTAAAAAATAATTTAAAACTTGAAAAACTTATAATTAATCAAGATTACTCAAAACTA
AAAGAAAATTTTGAAAGTTGAGCAAGAAATATCAGATATGATTTTTTTGTTGAAATATCAAAGAAATATAATATTCAAAA
TGTACTTATAGCACACAATAGAAATGATTTAGTTGAAACATTTTTATTACAACAAGATAGAAAAGGATATGTTAAACATT
ATGGTTTAAATAAGACTTCTACTTATAAAGAAATAACAATTGTTAGACCGATGCTAAATATTTTAAAATCTGAAATTCTT
AAATCATTAAAAGAAGAAAATATAGCATTTGTCATTGATTCAACTAATGAAGATAAAAAATATAAAAGAAATAAAATAAG
AGCAAATCTATCTGAATCCACTTTTAATGATTTTGAAGAGACAATAAAAAAATTAAATAAAAAATTAGAAATAATAAATT
TAGAAGTTAATTGATATGTAAATAATAATATGTCAGCTGATGAATTAAAAATAAATAAAAATTTACAAGACCAAGATCTA
GAGTTTATTCAAAGAACAATTTATAAATGATTAGAAGTAATTAAAAAAGATTTTGTTATCCAGAATAGAAGAAACAAAAC
TATTTTTGAAATTGCTAAAAATATTAAAGTTTCAGAAAAAGTTTTTTGAGAAATAAATATAGGTGAATACTCAATAATAA
AAGATTATGAAAACTTATTTATTATTGAAACAAAAGCTATACAACCTAAAACAATTTTAATCAAGTCAAAAGAAGATTTA
TACTTGTCAGAAGAATTTATAAATTGATTAGATTTATTAAACGCAATCAAAAGAAATAAGGAAAATTATCCCTACGTAAT
AACAAATGATTTTTTAACTTATAAATTAAATACTTATACGTTTGGTAAAAAAACAAATAGATATTTAATTGATAAAAAAA
TAAGATATAAAAATAGAATGTTAAAAGCTGTAGTTTACAGTACTAAAACAAAGAAAATCTTAAATACCATTAAGTAG

Upstream 100 bases:

>100_bases
AGAAGGCAATGAAGGTATGCAAATACTTAAACCGCTTATTTCTCATAAAGATGATGAATCTTATACAAATGAATTACTAA
GGTATTTTAAGGATTAAGGC

Downstream 100 bases:

>100_bases
AGTAATAATACAAATAATGGTATTATTAAAATAGAATAAAAATCTTGAGATGGAGGAATAAAATGAAGACTAAAAAAAGT
AAGTCAACTTTATGGTTTTG

Product: lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MERFKLNTTLSYLVAVSGGPDSMFMLNELIKMNIKEIVVCHVNYNFREDSWKDQKLVEDFCLKNNLKLEKLIINQDYSKL
KENFESWARNIRYDFFVEISKKYNIQNVLIAHNRNDLVETFLLQQDRKGYVKHYGLNKTSTYKEITIVRPMLNILKSEIL
KSLKEENIAFVIDSTNEDKKYKRNKIRANLSESTFNDFEETIKKLNKKLEIINLEVNWYVNNNMSADELKINKNLQDQDL
EFIQRTIYKWLEVIKKDFVIQNRRNKTIFEIAKNIKVSEKVFWEINIGEYSIIKDYENLFIIETKAIQPKTILIKSKEDL
YLSEEFINWLDLLNAIKRNKENYPYVITNDFLTYKLNTYTFGKKTNRYLIDKKIRYKNRMLKAVVYSTKTKKILNTIK

Sequences:

>Translated_398_residues
MERFKLNTTLSYLVAVSGGPDSMFMLNELIKMNIKEIVVCHVNYNFREDS*KDQKLVEDFCLKNNLKLEKLIINQDYSKL
KENFES*ARNIRYDFFVEISKKYNIQNVLIAHNRNDLVETFLLQQDRKGYVKHYGLNKTSTYKEITIVRPMLNILKSEIL
KSLKEENIAFVIDSTNEDKKYKRNKIRANLSESTFNDFEETIKKLNKKLEIINLEVN*YVNNNMSADELKINKNLQDQDL
EFIQRTIYK*LEVIKKDFVIQNRRNKTIFEIAKNIKVSEKVF*EINIGEYSIIKDYENLFIIETKAIQPKTILIKSKEDL
YLSEEFIN*LDLLNAIKRNKENYPYVITNDFLTYKLNTYTFGKKTNRYLIDKKIRYKNRMLKAVVYSTKTKKILNTIK
>Mature_398_residues
MERFKLNTTLSYLVAVSGGPDSMFMLNELIKMNIKEIVVCHVNYNFREDS*KDQKLVEDFCLKNNLKLEKLIINQDYSKL
KENFES*ARNIRYDFFVEISKKYNIQNVLIAHNRNDLVETFLLQQDRKGYVKHYGLNKTSTYKEITIVRPMLNILKSEIL
KSLKEENIAFVIDSTNEDKKYKRNKIRANLSESTFNDFEETIKKLNKKLEIINLEVN*YVNNNMSADELKINKNLQDQDL
EFIQRTIYK*LEVIKKDFVIQNRRNKTIFEIAKNIKVSEKVF*EINIGEYSIIKDYENLFIIETKAIQPKTILIKSKEDL
YLSEEFIN*LDLLNAIKRNKENYPYVITNDFLTYKLNTYTFGKKTNRYLIDKKIRYKNRMLKAVVYSTKTKKILNTIK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 46534; Mature: 46534

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFKLNTTLSYLVAVSGGPDSMFMLNELIKMNIKEIVVCHVNYNFREDSKDQKLVEDFC
CCCEEECCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHH
LKNNLKLEKLIINQDYSKLKENFESARNIRYDFFVEISKKYNIQNVLIAHNRNDLVETFL
HCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEECCHHHHHHHH
LQQDRKGYVKHYGLNKTSTYKEITIVRPMLNILKSEILKSLKEENIAFVIDSTNEDKKYK
HHCCCCCHHHCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHH
RNKIRANLSESTFNDFEETIKKLNKKLEIINLEVNYVNNNMSADELKINKNLQDQDLEFI
HHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCEEECCCCCHHHHHHH
QRTIYKLEVIKKDFVIQNRRNKTIFEIAKNIKVSEKVFEINIGEYSIIKDYENLFIIETK
HHHHHHHHHHHHHHHEECCCCCHHHHHHHCCEEECEEEEEECCCEEEEECCCCEEEEEEC
AIQPKTILIKSKEDLYLSEEFINLDLLNAIKRNKENYPYVITNDFLTYKLNTYTFGKKTN
CCCCEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEEEEEEECCCCC
RYLIDKKIRYKNRMLKAVVYSTKTKKILNTIK
CEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERFKLNTTLSYLVAVSGGPDSMFMLNELIKMNIKEIVVCHVNYNFREDSKDQKLVEDFC
CCCEEECCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHH
LKNNLKLEKLIINQDYSKLKENFESARNIRYDFFVEISKKYNIQNVLIAHNRNDLVETFL
HCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEECCHHHHHHHH
LQQDRKGYVKHYGLNKTSTYKEITIVRPMLNILKSEILKSLKEENIAFVIDSTNEDKKYK
HHCCCCCHHHCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHH
RNKIRANLSESTFNDFEETIKKLNKKLEIINLEVNYVNNNMSADELKINKNLQDQDLEFI
HHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCEEECCCCCHHHHHHH
QRTIYKLEVIKKDFVIQNRRNKTIFEIAKNIKVSEKVFEINIGEYSIIKDYENLFIIETK
HHHHHHHHHHHHHHHEECCCCCHHHHHHHCCEEECEEEEEECCCEEEEECCCCEEEEEEC
AIQPKTILIKSKEDLYLSEEFINLDLLNAIKRNKENYPYVITNDFLTYKLNTYTFGKKTN
CCCCEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEEEEEEECCCCC
RYLIDKKIRYKNRMLKAVVYSTKTKKILNTIK
CEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA