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The map label for this gene is gidB [H]

Identifier: 50365477

GI number: 50365477

Start: 768615

End: 769328

Strand: Reverse

Name: gidB [H]

Synonym: Mfl662

Alternate gene names: 50365477

Gene position: 769328-768615 (Counterclockwise)

Preceding gene: 50365478

Following gene: 50365476

Centisome position: 96.99

GC content: 24.23

Gene sequence:

>714_bases
ATGTGAACAATGTTTAATAATTGAAAAATTTTTGAAGACAATTTAGGCTTTGTTCCATCAGAAGAAATAAAAGATAAATT
AAATGAGTATTATAAAATACTTGTTGAAGAAAACTTAAAATATAATTTAACAAGAATAACAAATGAAGAAGATGTTTATG
AAAAACATTTTTTAGATTCTTTATTATTTACAAAGGAAACAAAAATTGATAATCAAAAAATTATAGATATTGGAACAGGG
CCTGGCTTCCCAGGAATAGTTTTAAAAATATTTTTTCCTGAAACAGATATTACTCTAATTGATTCAAATAATAAAAAAAT
AAATTTTCTTAACATTGTAATTAAAAAACTGAATCTAAAACAAATTGAAGCAAAACATGCAAGAGCAGAAGAATTGGCTA
GAATTGAAAACGAAAAATATGATATAGCAATTTCTAGAGCTGTTGCATATTTGGATGTGATTTTAGAATTAGCCGTTAGA
TTCTTAAAGATTCAAGGAAAATTAATTTTGTTAAAAGGACCAAGAGCTGATGAGGAAATAAAAAACTCAAAAAATATTGA
TCAAAAACTTAAAATAAAATTAACTAATAAACAGTCTCTTGCTGATACAGGTTTCGGAGAAAGAATAAATTTATTTTATG
AAAAAGAAAAATCAACTCCAGAACTTTATCCAAGAGAATATGCAAAAATAGTTAAAGAGAGCGGTAAAAAATAA

Upstream 100 bases:

>100_bases
ATTTATTTTAGTAAACAAAGGAACTTATGTTCCTTTTTATTTAGCAATCTTAAAAAGAAGGTATGTGCTATAATAATTTA
AGTTAAAAGAAGAAAAGGAG

Downstream 100 bases:

>100_bases
TGCATACAGAATTACAAATCGGTGATTTAGTTGTCTTTAAAAAATCGCACCCAAGTGGAACTGTAAAATGAGAACTAATT
CGCATTGGTGCGCTATACAA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MWTMFNNWKIFEDNLGFVPSEEIKDKLNEYYKILVEENLKYNLTRITNEEDVYEKHFLDSLLFTKETKIDNQKIIDIGTG
PGFPGIVLKIFFPETDITLIDSNNKKINFLNIVIKKLNLKQIEAKHARAEELARIENEKYDIAISRAVAYLDVILELAVR
FLKIQGKLILLKGPRADEEIKNSKNIDQKLKIKLTNKQSLADTGFGERINLFYEKEKSTPELYPREYAKIVKESGKK

Sequences:

>Translated_237_residues
M*TMFNN*KIFEDNLGFVPSEEIKDKLNEYYKILVEENLKYNLTRITNEEDVYEKHFLDSLLFTKETKIDNQKIIDIGTG
PGFPGIVLKIFFPETDITLIDSNNKKINFLNIVIKKLNLKQIEAKHARAEELARIENEKYDIAISRAVAYLDVILELAVR
FLKIQGKLILLKGPRADEEIKNSKNIDQKLKIKLTNKQSLADTGFGERINLFYEKEKSTPELYPREYAKIVKESGKK
>Mature_237_residues
M*TMFNN*KIFEDNLGFVPSEEIKDKLNEYYKILVEENLKYNLTRITNEEDVYEKHFLDSLLFTKETKIDNQKIIDIGTG
PGFPGIVLKIFFPETDITLIDSNNKKINFLNIVIKKLNLKQIEAKHARAEELARIENEKYDIAISRAVAYLDVILELAVR
FLKIQGKLILLKGPRADEEIKNSKNIDQKLKIKLTNKQSLADTGFGERINLFYEKEKSTPELYPREYAKIVKESGKK

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=165, Percent_Identity=34.5454545454545, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 27254; Mature: 27254

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMFNNKIFEDNLGFVPSEEIKDKLNEYYKILVEENLKYNLTRITNEEDVYEKHFLDSLL
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHH
FTKETKIDNQKIIDIGTGPGFPGIVLKIFFPETDITLIDSNNKKINFLNIVIKKLNLKQI
HHHHCCCCCCEEEEECCCCCCCCEEEEEEECCCCEEEEECCCCEEEHHHHHHHHCCHHHH
EAKHARAEELARIENEKYDIAISRAVAYLDVILELAVRFLKIQGKLILLKGPRADEEIKN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHH
SKNIDQKLKIKLTNKQSLADTGFGERINLFYEKEKSTPELYPREYAKIVKESGKK
CCCCCCEEEEEECCCCHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MTMFNNKIFEDNLGFVPSEEIKDKLNEYYKILVEENLKYNLTRITNEEDVYEKHFLDSLL
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHH
FTKETKIDNQKIIDIGTGPGFPGIVLKIFFPETDITLIDSNNKKINFLNIVIKKLNLKQI
HHHHCCCCCCEEEEECCCCCCCCEEEEEEECCCCEEEEECCCCEEEHHHHHHHHCCHHHH
EAKHARAEELARIENEKYDIAISRAVAYLDVILELAVRFLKIQGKLILLKGPRADEEIKN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHH
SKNIDQKLKIKLTNKQSLADTGFGERINLFYEKEKSTPELYPREYAKIVKESGKK
CCCCCCEEEEEECCCCHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA