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The map label for this gene is cls [H]

Identifier: 50365442

GI number: 50365442

Start: 732972

End: 734537

Strand: Reverse

Name: cls [H]

Synonym: Mfl626

Alternate gene names: 50365442

Gene position: 734537-732972 (Counterclockwise)

Preceding gene: 50365443

Following gene: 50365441

Centisome position: 92.6

GC content: 27.2

Gene sequence:

>1566_bases
ATGATAATGAAAAAACCGTTTGTAGCGACAGCTTCTTTAATTGCAATGTTCAGTATTGGAGGCGCTTTATATGTTGCAAT
CAATTTATGTTTAAACTTCTTTATACCTACACCATTGCCGTTTATTTTTTTTATATCAGCAACTCACATAGTTTCAATGG
TATGAGCAATTGTTGTTTTATGTAATAGAAAACGAAGAATTGAAACTAGAATTAGATGAGCAATATTTATAATAGTAATT
CCATTTTTTGGTATTATGTCTTACATATTTTTAGGTAGGGTTTACAAATATAATAAAAATAAGAATTATTTATATAACAA
AAACTCAGTTAGTGTTCTACAACCAAAAGTACAATATGACATTGAAAATCTAAAAGAAATTGAAAGACTAAATCCAGAGT
TTAAGAGATCATTTATGATGACTTTTGAACAACAAAGAGAAGGTATTTATGCAAATACTGAAATAGAATATTTAAAATCA
GGAAATGAATATTTTTCAAATTTATTAAATGATATAAAAAATGCTAAAGAGTATGTAATGATTAATTGTTACATCATTTC
TGAAGGTGAATTTTTGAGTAAACTAACAAGCTTACTAGTTGAAAAAATGAGTGAAGGTGTTAGAGTATACATAATTTATG
ATTTCTTAGGAAGTTATGGAAGATTTACAACAAGTAAAAAAAGATTAATTCAAGAGGGTGCAAACTTAATTGCTTACTCA
CCGATACATTTCCCATTTTTAAAATGAAATGCAAATTATAGAGATCACAGAAAAGATATTTCAATTGACGGCCAAATAGG
ATATTTAGGTGGAGTTAACATTTCAGATGAATACATAAATAAAAGTGGAGTTTTTGGTTTCTGAAACGATTCAGCAATAA
GAATTAATGGCGAAGGAGTTCAAGAAATTGAAGCAATATTCCAAAAAGACTGAAATTTTTATGTAACTAAAAAACAAAAG
AAAATTGAAAAATTAGAACCTAAATTTGGTAAAGCAATCAGAAAACGTTATACAACTGATGAATTTATTCAGATTGTTTC
TGATGGACCAAATCATGACAGACCTATTTGCTTAGAACTATTATTAAATTTAATTCATTCAGCGCAAAATAGAATTTGAC
TAAAGTCTCCTTACTTTATACCACCCCCAGAAATTATAAATGCGTTATGTAATGCTGCATCTACTGGATTGGATGTTCGT
ATTTTATTACCAGGAAGATCAGACAAATTTTTATTGTTAGAAGTTTCTAAACAATGAACTAAAAAAATGTTTGAAAACGG
CGTTAAAATATATTCAATGAATGATACTTTTATACATGAAAAAGCTTATATTTTTGATGAAGCTATATCATTTACTGGAA
GTTCTAATCTTGACTATAGAGCTCTATTTTGCGATCAACAAACTATGGCTCTAATAAGATCAAATAATATGAACAAAAAG
ATTGAAGAGAAAATGATTCATGACATGGATAAATCATTTGAATATAAATTTATGCCAAACAAAGATTTACCATTGTGAAA
AAGAGCTATTGTTAAAGTCTATAATATTATGGCCCCACTTTTATAA

Upstream 100 bases:

>100_bases
CGACTAAAGATATTTCGCCATTTTATTTTAATAGTATTATTTCAAAATTAAATAATTGATTAACAAGTAAAAGCAAAATG
TTTAGATTTAAGGAAGGTGC

Downstream 100 bases:

>100_bases
GGAGTTTTATGAATATAGAAAAAGTAGTATTTAATAAAATTAATAAATCTTACCAAGGCTTTAAGATGCTAGATGCATTT
TTGTTTGATGATGATTTTAA

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MIMKKPFVATASLIAMFSIGGALYVAINLCLNFFIPTPLPFIFFISATHIVSMVWAIVVLCNRKRRIETRIRWAIFIIVI
PFFGIMSYIFLGRVYKYNKNKNYLYNKNSVSVLQPKVQYDIENLKEIERLNPEFKRSFMMTFEQQREGIYANTEIEYLKS
GNEYFSNLLNDIKNAKEYVMINCYIISEGEFLSKLTSLLVEKMSEGVRVYIIYDFLGSYGRFTTSKKRLIQEGANLIAYS
PIHFPFLKWNANYRDHRKDISIDGQIGYLGGVNISDEYINKSGVFGFWNDSAIRINGEGVQEIEAIFQKDWNFYVTKKQK
KIEKLEPKFGKAIRKRYTTDEFIQIVSDGPNHDRPICLELLLNLIHSAQNRIWLKSPYFIPPPEIINALCNAASTGLDVR
ILLPGRSDKFLLLEVSKQWTKKMFENGVKIYSMNDTFIHEKAYIFDEAISFTGSSNLDYRALFCDQQTMALIRSNNMNKK
IEEKMIHDMDKSFEYKFMPNKDLPLWKRAIVKVYNIMAPLL

Sequences:

>Translated_521_residues
MIMKKPFVATASLIAMFSIGGALYVAINLCLNFFIPTPLPFIFFISATHIVSMV*AIVVLCNRKRRIETRIR*AIFIIVI
PFFGIMSYIFLGRVYKYNKNKNYLYNKNSVSVLQPKVQYDIENLKEIERLNPEFKRSFMMTFEQQREGIYANTEIEYLKS
GNEYFSNLLNDIKNAKEYVMINCYIISEGEFLSKLTSLLVEKMSEGVRVYIIYDFLGSYGRFTTSKKRLIQEGANLIAYS
PIHFPFLK*NANYRDHRKDISIDGQIGYLGGVNISDEYINKSGVFGF*NDSAIRINGEGVQEIEAIFQKD*NFYVTKKQK
KIEKLEPKFGKAIRKRYTTDEFIQIVSDGPNHDRPICLELLLNLIHSAQNRI*LKSPYFIPPPEIINALCNAASTGLDVR
ILLPGRSDKFLLLEVSKQ*TKKMFENGVKIYSMNDTFIHEKAYIFDEAISFTGSSNLDYRALFCDQQTMALIRSNNMNKK
IEEKMIHDMDKSFEYKFMPNKDLPL*KRAIVKVYNIMAPLL
>Mature_521_residues
MIMKKPFVATASLIAMFSIGGALYVAINLCLNFFIPTPLPFIFFISATHIVSMV*AIVVLCNRKRRIETRIR*AIFIIVI
PFFGIMSYIFLGRVYKYNKNKNYLYNKNSVSVLQPKVQYDIENLKEIERLNPEFKRSFMMTFEQQREGIYANTEIEYLKS
GNEYFSNLLNDIKNAKEYVMINCYIISEGEFLSKLTSLLVEKMSEGVRVYIIYDFLGSYGRFTTSKKRLIQEGANLIAYS
PIHFPFLK*NANYRDHRKDISIDGQIGYLGGVNISDEYINKSGVFGF*NDSAIRINGEGVQEIEAIFQKD*NFYVTKKQK
KIEKLEPKFGKAIRKRYTTDEFIQIVSDGPNHDRPICLELLLNLIHSAQNRI*LKSPYFIPPPEIINALCNAASTGLDVR
ILLPGRSDKFLLLEVSKQ*TKKMFENGVKIYSMNDTFIHEKAYIFDEAISFTGSSNLDYRALFCDQQTMALIRSNNMNKK
IEEKMIHDMDKSFEYKFMPNKDLPL*KRAIVKVYNIMAPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=421, Percent_Identity=22.8028503562945, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787007, Length=340, Percent_Identity=24.1176470588235, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 59136; Mature: 59136

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKKPFVATASLIAMFSIGGALYVAINLCLNFFIPTPLPFIFFISATHIVSMVAIVVLC
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
NRKRRIETRIRAIFIIVIPFFGIMSYIFLGRVYKYNKNKNYLYNKNSVSVLQPKVQYDIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCCEEECCHHHHHHH
NLKEIERLNPEFKRSFMMTFEQQREGIYANTEIEYLKSGNEYFSNLLNDIKNAKEYVMIN
HHHHHHHCCHHHHHHHHHHHHHHHCCCEECCHHHHHHCCHHHHHHHHHHHHCCCEEEEEE
CYIISEGEFLSKLTSLLVEKMSEGVRVYIIYDFLGSYGRFTTSKKRLIQEGANLIAYSPI
EEEEECCHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCHHHHHHHHCCCCEEEECCC
HFPFLKNANYRDHRKDISIDGQIGYLGGVNISDEYINKSGVFGFNDSAIRINGEGVQEIE
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHCCCCCCCCCCCCEEEECCCCHHHHH
AIFQKDNFYVTKKQKKIEKLEPKFGKAIRKRYTTDEFIQIVSDGPNHDRPICLELLLNLI
HHHHCCCEEEECHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
HSAQNRILKSPYFIPPPEIINALCNAASTGLDVRILLPGRSDKFLLLEVSKQTKKMFENG
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECHHHHHHHHCC
VKIYSMNDTFIHEKAYIFDEAISFTGSSNLDYRALFCDQQTMALIRSNNMNKKIEEKMIH
CEEEECCCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHCCCCCHHHHHHHHH
DMDKSFEYKFMPNKDLPLKRAIVKVYNIMAPLL
HHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIMKKPFVATASLIAMFSIGGALYVAINLCLNFFIPTPLPFIFFISATHIVSMVAIVVLC
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
NRKRRIETRIRAIFIIVIPFFGIMSYIFLGRVYKYNKNKNYLYNKNSVSVLQPKVQYDIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCCEEECCHHHHHHH
NLKEIERLNPEFKRSFMMTFEQQREGIYANTEIEYLKSGNEYFSNLLNDIKNAKEYVMIN
HHHHHHHCCHHHHHHHHHHHHHHHCCCEECCHHHHHHCCHHHHHHHHHHHHCCCEEEEEE
CYIISEGEFLSKLTSLLVEKMSEGVRVYIIYDFLGSYGRFTTSKKRLIQEGANLIAYSPI
EEEEECCHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCHHHHHHHHCCCCEEEECCC
HFPFLKNANYRDHRKDISIDGQIGYLGGVNISDEYINKSGVFGFNDSAIRINGEGVQEIE
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHCCCCCCCCCCCCEEEECCCCHHHHH
AIFQKDNFYVTKKQKKIEKLEPKFGKAIRKRYTTDEFIQIVSDGPNHDRPICLELLLNLI
HHHHCCCEEEECHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
HSAQNRILKSPYFIPPPEIINALCNAASTGLDVRILLPGRSDKFLLLEVSKQTKKMFENG
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECHHHHHHHHCC
VKIYSMNDTFIHEKAYIFDEAISFTGSSNLDYRALFCDQQTMALIRSNNMNKKIEEKMIH
CEEEECCCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHCCCCCHHHHHHHHH
DMDKSFEYKFMPNKDLPLKRAIVKVYNIMAPLL
HHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA