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The map label for this gene is alaS [H]

Identifier: 50365429

GI number: 50365429

Start: 716265

End: 718943

Strand: Reverse

Name: alaS [H]

Synonym: Mfl613

Alternate gene names: 50365429

Gene position: 718943-716265 (Counterclockwise)

Preceding gene: 50365431

Following gene: 50365428

Centisome position: 90.64

GC content: 27.36

Gene sequence:

>2679_bases
ATGAAAAAATTAACAACAAATGAAATTAGAAAAATGTGATTAGATTTTTTTAAAGAAAAAAACCACCATTTTTTAGAACC
AGTTAGTTTAATACCTGTAGAAGACCCTTCATTACTATGAATTAACTCTGGTGTAGCGACACTAAAACCTTATTTTGATG
GAAGAAAAACACCGCCCTCACCTAGGCTAACTAACTCACAAAAAGCAATTAGAACTAATGATATTGAAAATGTAGGTGTT
ACAGCTAGACACCATACAATGTTTGAAATGTTAGGTAATTTTTCAATTGGAGATTATTTTAAAAAAGAAGCAATATCATT
TGCATGAGAATTATTAACAAGTGATAAATGGTTTGCTATTCCTGTAGAAAAATTATATATAACTGTATTTAATGAAGATA
TTGAGGCATATGAATATTGAATTAATGATATTGGAATTAAACAAGACCATATTTTTAGATTAACAAGAGATACAAACTTT
TGAGACGTAGGTCAAGGACCTTGTGGTCCAAATACTGAAATTTTCTTTGATCGTGGCGAAAAATGAGATAAAGAAAATAT
TGGTCCAAGATTATTAGCTGAAGATATTGAAAACGATAGATACATTGAAATTTGAAATATTGTTTTCTCTCAATTTAATA
ATGATGGTTTTAATAACTATTCAGAGCTACCAAGAAAAAATATAGATACTGGTGCTGGCCTAGAAAGAATAGCTTCTATT
TTTCAAGATACTCCAACAAACTTTGAAACAGATATTTTCTGACCAACGATCAAACAAATTGAAAATATTTGTGATGCTAA
TTTCAAATACTCAATTGAAAATTACTTTGATGAAAAAGCAGAGCAAACTAAAATTAATACAGCTTTCAAAGTTATTGCTG
ATCATGTAAGAGCAACAAGTTTTGCTATTGCTGATGGTGTTTTTCCAGGTAACAAAGATAGAGGATATATAATTCGCCGT
TTAATTCGCCGTGCTTTAATGAAAGGTATGGAGTTAGGTATTAATGGGCCATTTTTAAGCACATTAGTAATAAATGTTAT
TGATGTCATGAAAGATTTTTATCCATATTTATTAGAAAAACAAAATTTAATTGAAACAACAATTTTAATTGAAGAAGAAA
AATTCTTAAAAACTTTATCAAAAGGTTATGAGGCATTAAATAAAATGATTGAAAATGACAAAAAAGTAACAGGTAAAAAT
GCATTACTTTTATTTGAATCATATGGTTATCCAATAGAACAAACAATTGAAATTGCTGAAGACAGAAAAGTTAAGGTAGA
AATTGAAGAATTTAATAGTTTATTAGAACAAGCAAAAGAACAAGCTCGTAATGCGAGAAAAGATTTAAAAGCTTGAGATA
AACAAAATGAAATCTTTACAAAAATAAATGTTGAATCAGAGTTTACTGGTTGAGAAGAAACAAGCCATAAAAATGCAAAA
ATTGTATATATATTCACAGACGATGAAATTCTTACTGAAGCTACAGATAAAGAAGTTTATGTTATCTTAGATAAAACTCC
TTTTTATGCTGAAAAAGGTGGCCAAGCTGCTGATAAAGGTTATTTAGTAAATAAAGAAGCAAGATGTGAAGTAATTGATA
CACAACAAGGACCAAATCATCAACATATTCATAAAATTCTATTAGATGGTTCAATTAAAATTGGAGATCAAATCGATGCA
TTTGTAGATGAAACAAAAAGAACATTAACAATGAAAAATCATTCAGGTACTCATTTAATTCACTCTGCATTAAGAGTTGT
ATTGGGAGAAACTGTTATGCAATCAGGTTCATATAATGATGAACATGGATTACGTATGGACTTCACATATAATGACTCAA
TAAAAGCAGAAGAATTAATTGCAGCAGAGAAATTAGTTTTAGATAAAATTAATGAAAAAATAAATAGAGAAGTTTATTTC
TGTTCAATGAAAGATGCAGTAAGCAAATATGGTGCTTTAGCATTCTTTACTGAAAAATATGATGACATTGTAAGAGTTGT
AAAATTTGGAGATTTCTCAAGTGAATTATGTGGTGGTACTCATGTTGATAATACAATTGACATAGAAGACTTTATGATAA
CAGGTTTAGAATCAAAAGGTAGTGGAGTTTACAGGGTTAAGTGTTTAACATCAAAAAAAGCAATTAATGAATATTTAAAT
ACAGAATTTAATAAACTTTTAAAATTGATTCAAGAACAAAATTCTAAATACGAAAATACTAAATTAATTCAGGCTGACCA
AAACATTGAAAATATTGTTAAAGAATCAGTTACTAAAACAATTTCAAAAGAGTCAATTCAAGAATTGAAATTGATATTAG
AAAAGTTATCAGCTGCTTTAAAAATATATGAAAGAAAAATTGAAGATTTATTAACTTCTAAAAAATTAGAACAATTTAAA
GCTTTTGAACCTATAACTAATGACAAAGGTGTAGGAACAATTGAACAACAAGTGAATGGTTTGAATATTAAAGATATGAA
AAACCTTGTAGATGAGTATAAAAATAAATTTGAAAAATTAATTATAATATTAACTTCTGAAACTGAAGAAGGAAACTTTG
TGGTTGTTGGAGTTAGTGAAAAAATTCAAAATGAATATTCAGCTATAGAAATATTTAAAAACTTAACAATATCTCCAAAA
GGTGGAGGAAACTCAAGTTTAGCGCAAGGAAAATTTTAA

Upstream 100 bases:

>100_bases
GAATAAGTAGATATAAATATATTTTATCAAAATTTAGATAAAAATATTTTCTCTTTGATTTTGTTAAAGGTTATAATTAA
TTTTAAAGGACTGATATTTA

Downstream 100 bases:

>100_bases
TATCTAAAATCGAATTATTTTTGTTTTTTCCCACTATTGTTGTATAATTTTAAGAGAAATACAGAAAGGAATAAGTAAAA
TGAACATTATAAAAAGAGCA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 892; Mature: 892

Protein sequence:

>892_residues
MKKLTTNEIRKMWLDFFKEKNHHFLEPVSLIPVEDPSLLWINSGVATLKPYFDGRKTPPSPRLTNSQKAIRTNDIENVGV
TARHHTMFEMLGNFSIGDYFKKEAISFAWELLTSDKWFAIPVEKLYITVFNEDIEAYEYWINDIGIKQDHIFRLTRDTNF
WDVGQGPCGPNTEIFFDRGEKWDKENIGPRLLAEDIENDRYIEIWNIVFSQFNNDGFNNYSELPRKNIDTGAGLERIASI
FQDTPTNFETDIFWPTIKQIENICDANFKYSIENYFDEKAEQTKINTAFKVIADHVRATSFAIADGVFPGNKDRGYIIRR
LIRRALMKGMELGINGPFLSTLVINVIDVMKDFYPYLLEKQNLIETTILIEEEKFLKTLSKGYEALNKMIENDKKVTGKN
ALLLFESYGYPIEQTIEIAEDRKVKVEIEEFNSLLEQAKEQARNARKDLKAWDKQNEIFTKINVESEFTGWEETSHKNAK
IVYIFTDDEILTEATDKEVYVILDKTPFYAEKGGQAADKGYLVNKEARCEVIDTQQGPNHQHIHKILLDGSIKIGDQIDA
FVDETKRTLTMKNHSGTHLIHSALRVVLGETVMQSGSYNDEHGLRMDFTYNDSIKAEELIAAEKLVLDKINEKINREVYF
CSMKDAVSKYGALAFFTEKYDDIVRVVKFGDFSSELCGGTHVDNTIDIEDFMITGLESKGSGVYRVKCLTSKKAINEYLN
TEFNKLLKLIQEQNSKYENTKLIQADQNIENIVKESVTKTISKESIQELKLILEKLSAALKIYERKIEDLLTSKKLEQFK
AFEPITNDKGVGTIEQQVNGLNIKDMKNLVDEYKNKFEKLIIILTSETEEGNFVVVGVSEKIQNEYSAIEIFKNLTISPK
GGGNSSLAQGKF

Sequences:

>Translated_892_residues
MKKLTTNEIRKM*LDFFKEKNHHFLEPVSLIPVEDPSLL*INSGVATLKPYFDGRKTPPSPRLTNSQKAIRTNDIENVGV
TARHHTMFEMLGNFSIGDYFKKEAISFA*ELLTSDKWFAIPVEKLYITVFNEDIEAYEY*INDIGIKQDHIFRLTRDTNF
*DVGQGPCGPNTEIFFDRGEK*DKENIGPRLLAEDIENDRYIEI*NIVFSQFNNDGFNNYSELPRKNIDTGAGLERIASI
FQDTPTNFETDIF*PTIKQIENICDANFKYSIENYFDEKAEQTKINTAFKVIADHVRATSFAIADGVFPGNKDRGYIIRR
LIRRALMKGMELGINGPFLSTLVINVIDVMKDFYPYLLEKQNLIETTILIEEEKFLKTLSKGYEALNKMIENDKKVTGKN
ALLLFESYGYPIEQTIEIAEDRKVKVEIEEFNSLLEQAKEQARNARKDLKA*DKQNEIFTKINVESEFTG*EETSHKNAK
IVYIFTDDEILTEATDKEVYVILDKTPFYAEKGGQAADKGYLVNKEARCEVIDTQQGPNHQHIHKILLDGSIKIGDQIDA
FVDETKRTLTMKNHSGTHLIHSALRVVLGETVMQSGSYNDEHGLRMDFTYNDSIKAEELIAAEKLVLDKINEKINREVYF
CSMKDAVSKYGALAFFTEKYDDIVRVVKFGDFSSELCGGTHVDNTIDIEDFMITGLESKGSGVYRVKCLTSKKAINEYLN
TEFNKLLKLIQEQNSKYENTKLIQADQNIENIVKESVTKTISKESIQELKLILEKLSAALKIYERKIEDLLTSKKLEQFK
AFEPITNDKGVGTIEQQVNGLNIKDMKNLVDEYKNKFEKLIIILTSETEEGNFVVVGVSEKIQNEYSAIEIFKNLTISPK
GGGNSSLAQGKF
>Mature_892_residues
MKKLTTNEIRKM*LDFFKEKNHHFLEPVSLIPVEDPSLL*INSGVATLKPYFDGRKTPPSPRLTNSQKAIRTNDIENVGV
TARHHTMFEMLGNFSIGDYFKKEAISFA*ELLTSDKWFAIPVEKLYITVFNEDIEAYEY*INDIGIKQDHIFRLTRDTNF
*DVGQGPCGPNTEIFFDRGEK*DKENIGPRLLAEDIENDRYIEI*NIVFSQFNNDGFNNYSELPRKNIDTGAGLERIASI
FQDTPTNFETDIF*PTIKQIENICDANFKYSIENYFDEKAEQTKINTAFKVIADHVRATSFAIADGVFPGNKDRGYIIRR
LIRRALMKGMELGINGPFLSTLVINVIDVMKDFYPYLLEKQNLIETTILIEEEKFLKTLSKGYEALNKMIENDKKVTGKN
ALLLFESYGYPIEQTIEIAEDRKVKVEIEEFNSLLEQAKEQARNARKDLKA*DKQNEIFTKINVESEFTG*EETSHKNAK
IVYIFTDDEILTEATDKEVYVILDKTPFYAEKGGQAADKGYLVNKEARCEVIDTQQGPNHQHIHKILLDGSIKIGDQIDA
FVDETKRTLTMKNHSGTHLIHSALRVVLGETVMQSGSYNDEHGLRMDFTYNDSIKAEELIAAEKLVLDKINEKINREVYF
CSMKDAVSKYGALAFFTEKYDDIVRVVKFGDFSSELCGGTHVDNTIDIEDFMITGLESKGSGVYRVKCLTSKKAINEYLN
TEFNKLLKLIQEQNSKYENTKLIQADQNIENIVKESVTKTISKESIQELKLILEKLSAALKIYERKIEDLLTSKKLEQFK
AFEPITNDKGVGTIEQQVNGLNIKDMKNLVDEYKNKFEKLIIILTSETEEGNFVVVGVSEKIQNEYSAIEIFKNLTISPK
GGGNSSLAQGKF

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=858, Percent_Identity=32.4009324009324, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI38569417, Length=956, Percent_Identity=28.2426778242678, Blast_Score=314, Evalue=3e-85,
Organism=Escherichia coli, GI1789048, Length=900, Percent_Identity=37.4444444444444, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17506981, Length=849, Percent_Identity=31.4487632508834, Blast_Score=347, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17536681, Length=752, Percent_Identity=30.5851063829787, Blast_Score=276, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6324911, Length=773, Percent_Identity=35.1875808538163, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24582809, Length=970, Percent_Identity=31.4432989690722, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45552267, Length=970, Percent_Identity=31.4432989690722, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24658214, Length=812, Percent_Identity=24.384236453202, Blast_Score=225, Evalue=9e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 100387; Mature: 100387

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTTNEIRKMLDFFKEKNHHFLEPVSLIPVEDPSLLINSGVATLKPYFDGRKTPPSPR
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECCCHHCCCCCCCCCCCCCCC
LTNSQKAIRTNDIENVGVTARHHTMFEMLGNFSIGDYFKKEAISFAELLTSDKWFAIPVE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHH
KLYITVFNEDIEAYEYINDIGIKQDHIFRLTRDTNFDVGQGPCGPNTEIFFDRGEKDKEN
HEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCHHH
IGPRLLAEDIENDRYIEINIVFSQFNNDGFNNYSELPRKNIDTGAGLERIASIFQDTPTN
CCHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCC
FETDIFPTIKQIENICDANFKYSIENYFDEKAEQTKINTAFKVIADHVRATSFAIADGVF
CCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PGNKDRGYIIRRLIRRALMKGMELGINGPFLSTLVINVIDVMKDFYPYLLEKQNLIETTI
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHE
LIEEEKFLKTLSKGYEALNKMIENDKKVTGKNALLLFESYGYPIEQTIEIAEDRKVKVEI
EECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEEH
EEFNSLLEQAKEQARNARKDLKADKQNEIFTKINVESEFTGEETSHKNAKIVYIFTDDEI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCHH
LTEATDKEVYVILDKTPFYAEKGGQAADKGYLVNKEARCEVIDTQQGPNHQHIHKILLDG
HHCCCCCEEEEEEECCCCHHCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHCC
SIKIGDQIDAFVDETKRTLTMKNHSGTHLIHSALRVVLGETVMQSGSYNDEHGLRMDFTY
CEEECCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
NDSIKAEELIAAEKLVLDKINEKINREVYFCSMKDAVSKYGALAFFTEKYDDIVRVVKFG
CCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHCC
DFSSELCGGTHVDNTIDIEDFMITGLESKGSGVYRVKCLTSKKAINEYLNTEFNKLLKLI
CCHHHHCCCCCCCCEEEHHHHEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
QEQNSKYENTKLIQADQNIENIVKESVTKTISKESIQELKLILEKLSAALKIYERKIEDL
HHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTSKKLEQFKAFEPITNDKGVGTIEQQVNGLNIKDMKNLVDEYKNKFEKLIIILTSETEE
HHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCC
GNFVVVGVSEKIQNEYSAIEIFKNLTISPKGGGNSSLAQGKF
CCEEEEECHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKLTTNEIRKMLDFFKEKNHHFLEPVSLIPVEDPSLLINSGVATLKPYFDGRKTPPSPR
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECCCHHCCCCCCCCCCCCCCC
LTNSQKAIRTNDIENVGVTARHHTMFEMLGNFSIGDYFKKEAISFAELLTSDKWFAIPVE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHH
KLYITVFNEDIEAYEYINDIGIKQDHIFRLTRDTNFDVGQGPCGPNTEIFFDRGEKDKEN
HEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCHHH
IGPRLLAEDIENDRYIEINIVFSQFNNDGFNNYSELPRKNIDTGAGLERIASIFQDTPTN
CCHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCC
FETDIFPTIKQIENICDANFKYSIENYFDEKAEQTKINTAFKVIADHVRATSFAIADGVF
CCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PGNKDRGYIIRRLIRRALMKGMELGINGPFLSTLVINVIDVMKDFYPYLLEKQNLIETTI
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHE
LIEEEKFLKTLSKGYEALNKMIENDKKVTGKNALLLFESYGYPIEQTIEIAEDRKVKVEI
EECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEEH
EEFNSLLEQAKEQARNARKDLKADKQNEIFTKINVESEFTGEETSHKNAKIVYIFTDDEI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCHH
LTEATDKEVYVILDKTPFYAEKGGQAADKGYLVNKEARCEVIDTQQGPNHQHIHKILLDG
HHCCCCCEEEEEEECCCCHHCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHCC
SIKIGDQIDAFVDETKRTLTMKNHSGTHLIHSALRVVLGETVMQSGSYNDEHGLRMDFTY
CEEECCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
NDSIKAEELIAAEKLVLDKINEKINREVYFCSMKDAVSKYGALAFFTEKYDDIVRVVKFG
CCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHCC
DFSSELCGGTHVDNTIDIEDFMITGLESKGSGVYRVKCLTSKKAINEYLNTEFNKLLKLI
CCHHHHCCCCCCCCEEEHHHHEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
QEQNSKYENTKLIQADQNIENIVKESVTKTISKESIQELKLILEKLSAALKIYERKIEDL
HHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTSKKLEQFKAFEPITNDKGVGTIEQQVNGLNIKDMKNLVDEYKNKFEKLIIILTSETEE
HHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCC
GNFVVVGVSEKIQNEYSAIEIFKNLTISPKGGGNSSLAQGKF
CCEEEEECHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA