Click here to switch to the map view.

The map label for this gene is ldh [H]

Identifier: 50365413

GI number: 50365413

Start: 694198

End: 695151

Strand: Reverse

Name: ldh [H]

Synonym: Mfl596

Alternate gene names: 50365413

Gene position: 695151-694198 (Counterclockwise)

Preceding gene: 50365414

Following gene: 50365412

Centisome position: 87.64

GC content: 34.07

Gene sequence:

>954_bases
ATGAAAAAAACTTCAAATAAAGTTGTATTAGTTGGTACTGGTGCAGTTGGTATGTCATTTATTTATTCAGCAGTAAACCA
AGGTTTAGCTGAAGAATACGTATTAATCGATGTTAATACAAAAGCTGCAGAAGGGAACGCAATTGATATCCAAGATACAA
TGGCTGTTTTAGACAAACCTTTTACAATTAAAGCTGGAACTTACGAAGATTGTAAAGATGCAGATTTAATTGTTATTACT
GCTGGGCGCCCACAAAGACCTGGAGAAACAAGATTAGAATTAATTGCAGATAACTCAAGAATTATGAAAGGTATAGCTGA
AGCTATTAAAGCATCAGGATTCAATGGAGTAACTGTTATTGCTTCAAACCCATGTGATGTTTTAACTACTGTTTATCAAC
AAGTAACTGGTTATGATGAACATTCAGTTGTAGGAGCAGGAACAACACTTGATTCAGCTAGATTAAGAAGATTAGTTGCT
GAAAAATTAAATGTGGCTCCTAAATCAGTTAATGCTTATATTATGGGAGAACATGGAGATTCATCAGTTGCTGCGTACTC
AAAAGCAACAGTTATGGGTCAACCAATTAGCAAATACTTAGCAGAAGGAAAAATTACTGAAGCTGATTTAGAAGAATGTT
GAACTAGAGCTATTAGAATGGCTTATGAAATTATTGAACGTAAAGGTGCTACTTACTACGGAATTGGTGTTTGTTTAAAT
GCAATTTCATCAGCAATCTTGAGAGACGAAAAAACTACATTCATGGTAGGTGCTAAACTAAATGGTGAATATGGACAAAA
AGGGTTCTATACTGGAGTTCCTGTTATTTTAGGGTCAAAAGGATGAGAAACAATCATTGAATGAGATCTATCAGATGCAG
AAAAAGCAGCATTTAAAAAATCATGTGATGCTTTAGATGCAACTTACCAAAAAGCAAAAGAAGCTATTGCTTAA

Upstream 100 bases:

>100_bases
TAAAAAACAAAAAATATTTTCTCTATCAAAAGAGTTAATTTTTTATAATAGAGAGAATATAATTATATGTGTAGAGAGAA
ATAATAAGGAGATATTAAAT

Downstream 100 bases:

>100_bases
TTAAAATTAATAATAAGATGTTAATCATCTTATTTTTTTTGCGCTTTTTGCTTAATTATAAATGAGGTGAACAAATGCTA
AACCAAATCAAAGAAGAAGT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKKTSNKVVLVGTGAVGMSFIYSAVNQGLAEEYVLIDVNTKAAEGNAIDIQDTMAVLDKPFTIKAGTYEDCKDADLIVIT
AGRPQRPGETRLELIADNSRIMKGIAEAIKASGFNGVTVIASNPCDVLTTVYQQVTGYDEHSVVGAGTTLDSARLRRLVA
EKLNVAPKSVNAYIMGEHGDSSVAAYSKATVMGQPISKYLAEGKITEADLEECWTRAIRMAYEIIERKGATYYGIGVCLN
AISSAILRDEKTTFMVGAKLNGEYGQKGFYTGVPVILGSKGWETIIEWDLSDAEKAAFKKSCDALDATYQKAKEAIA

Sequences:

>Translated_317_residues
MKKTSNKVVLVGTGAVGMSFIYSAVNQGLAEEYVLIDVNTKAAEGNAIDIQDTMAVLDKPFTIKAGTYEDCKDADLIVIT
AGRPQRPGETRLELIADNSRIMKGIAEAIKASGFNGVTVIASNPCDVLTTVYQQVTGYDEHSVVGAGTTLDSARLRRLVA
EKLNVAPKSVNAYIMGEHGDSSVAAYSKATVMGQPISKYLAEGKITEADLEEC*TRAIRMAYEIIERKGATYYGIGVCLN
AISSAILRDEKTTFMVGAKLNGEYGQKGFYTGVPVILGSKG*ETIIE*DLSDAEKAAFKKSCDALDATYQKAKEAIA
>Mature_317_residues
MKKTSNKVVLVGTGAVGMSFIYSAVNQGLAEEYVLIDVNTKAAEGNAIDIQDTMAVLDKPFTIKAGTYEDCKDADLIVIT
AGRPQRPGETRLELIADNSRIMKGIAEAIKASGFNGVTVIASNPCDVLTTVYQQVTGYDEHSVVGAGTTLDSARLRRLVA
EKLNVAPKSVNAYIMGEHGDSSVAAYSKATVMGQPISKYLAEGKITEADLEEC*TRAIRMAYEIIERKGATYYGIGVCLN
AISSAILRDEKTTFMVGAKLNGEYGQKGFYTGVPVILGSKG*ETIIE*DLSDAEKAAFKKSCDALDATYQKAKEAIA

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=35.0819672131148, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=35.0819672131148, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI5031857, Length=304, Percent_Identity=35.1973684210526, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI260099723, Length=304, Percent_Identity=35.1973684210526, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI9257228, Length=303, Percent_Identity=35.3135313531353, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4504973, Length=303, Percent_Identity=35.3135313531353, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI47059044, Length=312, Percent_Identity=32.6923076923077, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI221136809, Length=312, Percent_Identity=32.6923076923077, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI15082234, Length=312, Percent_Identity=33.0128205128205, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI260099727, Length=222, Percent_Identity=36.036036036036, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI260099725, Length=224, Percent_Identity=36.6071428571429, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI207028494, Length=304, Percent_Identity=29.6052631578947, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI103472011, Length=279, Percent_Identity=21.505376344086, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI103472015, Length=185, Percent_Identity=21.6216216216216, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17535107, Length=293, Percent_Identity=37.2013651877133, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6322765, Length=146, Percent_Identity=33.5616438356164, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17136226, Length=308, Percent_Identity=31.8181818181818, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI45550422, Length=304, Percent_Identity=27.3026315789474, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24663599, Length=269, Percent_Identity=25.2788104089219, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 33471; Mature: 33471

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTSNKVVLVGTGAVGMSFIYSAVNQGLAEEYVLIDVNTKAAEGNAIDIQDTMAVLDKP
CCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHCCC
FTIKAGTYEDCKDADLIVITAGRPQRPGETRLELIADNSRIMKGIAEAIKASGFNGVTVI
EEEECCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEE
ASNPCDVLTTVYQQVTGYDEHSVVGAGTTLDSARLRRLVAEKLNVAPKSVNAYIMGEHGD
ECCCHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
SSVAAYSKATVMGQPISKYLAEGKITEADLEECTRAIRMAYEIIERKGATYYGIGVCLNA
CCHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHH
ISSAILRDEKTTFMVGAKLNGEYGQKGFYTGVPVILGSKGETIIEDLSDAEKAAFKKSCD
HHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
ALDATYQKAKEAIA
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKKTSNKVVLVGTGAVGMSFIYSAVNQGLAEEYVLIDVNTKAAEGNAIDIQDTMAVLDKP
CCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHCCC
FTIKAGTYEDCKDADLIVITAGRPQRPGETRLELIADNSRIMKGIAEAIKASGFNGVTVI
EEEECCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEE
ASNPCDVLTTVYQQVTGYDEHSVVGAGTTLDSARLRRLVAEKLNVAPKSVNAYIMGEHGD
ECCCHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
SSVAAYSKATVMGQPISKYLAEGKITEADLEECTRAIRMAYEIIERKGATYYGIGVCLNA
CCHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHH
ISSAILRDEKTTFMVGAKLNGEYGQKGFYTGVPVILGSKGETIIEDLSDAEKAAFKKSCD
HHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
ALDATYQKAKEAIA
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA