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The map label for this gene is thiI [H]

Identifier: 50365401

GI number: 50365401

Start: 682846

End: 684048

Strand: Reverse

Name: thiI [H]

Synonym: Mfl584

Alternate gene names: 50365401

Gene position: 684048-682846 (Counterclockwise)

Preceding gene: 50365402

Following gene: 50365400

Centisome position: 86.24

GC content: 27.35

Gene sequence:

>1203_bases
ATGAAAAGTATATTAGTAAGATATGGTGAGTTAACATTAAAAGGGAATAATAAACATATGTTTATTAGCAAACTTTTGGA
TAACATAAAATATAAATTAAAAAAATTTGATCAATCAAAAATTAAATTTATTAAAGATAATAATTCATTAGTTATTGATG
CAGAAGAAGAAATATTAGAAGATGTTCTTGAAGAATTAAAAACAGTTTTTGGTATATATTCATTATCAGTGATTGAAAAA
TGTGAAAAAGATTTAGATAAAATTGCTGAAAAAATCATTGAGATAGCCAAAAAATCTGAATATAAAAGATTTAAACTAGA
AATTACAAGAAAAGATAAAAGTTTTCCAATGACAAGTGCAGACTTAAAGTTAGCACTAGCTCCAATGGTTCTTAAGGCTG
TGGATAATCTTATTGTTGATGTTCATAATCCGGATTTGAAAATTGAAGTGCTTGTTAAAAAAGATGGTATTCAAATTTTT
TCAAAAAGAATACAAGGATTAAAAGGATTGCCTGTAGGTGTTAGTGGCAAAGGACTTTCATTATTAAGCGGTGGAATAGA
TTCACCTGTTGCTTCTTTCTTAACAATGAAAAGAGGTATGCATGTTGATTTCATCCATTTTATGACACCACCGCATACTT
CTCCAGAAGCTTTAGATAAAGTATTTGAACTTGCAAAAATTGTTTCAAAATATAATTCAAAGAAATTTAATCTTTATGTT
TGTGATTTTTCAATGTTATTGCAAGAACTACAACATATGCCAGAAGAAAGCTACAAAATAACAATTATGAGAAGAATGTT
TATGCGTATAGCTAACAAATTAGCCAAACAAAATGGGCAAGAAGCCTTAATAACAGGTGAAAGTTTAGGACAAGTTGCTT
CTCAAACAATTCAAAGTATAGATGTAATAAATTCAACAAGTGAACTGCCTATTTTAAGACCAGTTCTAACATACGATAAA
GAAGAAATTATATTAATTTCTAAATTTATTAAATCTTATGAGACATCTATTTTGCCTTTTGATGATGCATGTTCATTATT
TGTACCAAAACAACCAGTTACAAAACCTAAAAAATGAATGGCTGAACAGCAAGAAAATGCTATTCTATGAAATGAACTAT
TGGATTATACAATGGAAAATAAAATACAAAAATTTGTTTTTTGAAATGGGAGTTTCACAGAAGAAATCAACAAGGAGGAT
TAA

Upstream 100 bases:

>100_bases
AGCAAGAAAACTATACAGAATGTTTAAAAGATAATGTTTTAATTTTGAATAAATTAAATTCTCAAAATATAGCAGTTAGT
TAAAATAGAAATTAGGAAAA

Downstream 100 bases:

>100_bases
TGTCAAATTTCATACCCCTAATAAATGTGCGTAGTGTTTATAATTTTCAAGAGTCATTAATAAAAATTAATGACTATATT
TCTTTTGCGAAAAATGAAGG

Product: thiamin biosynthesis protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKSILVRYGELTLKGNNKHMFISKLLDNIKYKLKKFDQSKIKFIKDNNSLVIDAEEEILEDVLEELKTVFGIYSLSVIEK
CEKDLDKIAEKIIEIAKKSEYKRFKLEITRKDKSFPMTSADLKLALAPMVLKAVDNLIVDVHNPDLKIEVLVKKDGIQIF
SKRIQGLKGLPVGVSGKGLSLLSGGIDSPVASFLTMKRGMHVDFIHFMTPPHTSPEALDKVFELAKIVSKYNSKKFNLYV
CDFSMLLQELQHMPEESYKITIMRRMFMRIANKLAKQNGQEALITGESLGQVASQTIQSIDVINSTSELPILRPVLTYDK
EEIILISKFIKSYETSILPFDDACSLFVPKQPVTKPKKWMAEQQENAILWNELLDYTMENKIQKFVFWNGSFTEEINKED

Sequences:

>Translated_400_residues
MKSILVRYGELTLKGNNKHMFISKLLDNIKYKLKKFDQSKIKFIKDNNSLVIDAEEEILEDVLEELKTVFGIYSLSVIEK
CEKDLDKIAEKIIEIAKKSEYKRFKLEITRKDKSFPMTSADLKLALAPMVLKAVDNLIVDVHNPDLKIEVLVKKDGIQIF
SKRIQGLKGLPVGVSGKGLSLLSGGIDSPVASFLTMKRGMHVDFIHFMTPPHTSPEALDKVFELAKIVSKYNSKKFNLYV
CDFSMLLQELQHMPEESYKITIMRRMFMRIANKLAKQNGQEALITGESLGQVASQTIQSIDVINSTSELPILRPVLTYDK
EEIILISKFIKSYETSILPFDDACSLFVPKQPVTKPKK*MAEQQENAIL*NELLDYTMENKIQKFVF*NGSFTEEINKED
>Mature_400_residues
MKSILVRYGELTLKGNNKHMFISKLLDNIKYKLKKFDQSKIKFIKDNNSLVIDAEEEILEDVLEELKTVFGIYSLSVIEK
CEKDLDKIAEKIIEIAKKSEYKRFKLEITRKDKSFPMTSADLKLALAPMVLKAVDNLIVDVHNPDLKIEVLVKKDGIQIF
SKRIQGLKGLPVGVSGKGLSLLSGGIDSPVASFLTMKRGMHVDFIHFMTPPHTSPEALDKVFELAKIVSKYNSKKFNLYV
CDFSMLLQELQHMPEESYKITIMRRMFMRIANKLAKQNGQEALITGESLGQVASQTIQSIDVINSTSELPILRPVLTYDK
EEIILISKFIKSYETSILPFDDACSLFVPKQPVTKPKK*MAEQQENAIL*NELLDYTMENKIQKFVF*NGSFTEEINKED

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=384, Percent_Identity=27.34375, Blast_Score=141, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 45174; Mature: 45174

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSILVRYGELTLKGNNKHMFISKLLDNIKYKLKKFDQSKIKFIKDNNSLVIDAEEEILE
CCCCEEEECEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHEEEEECCCCEEEECHHHHHH
DVLEELKTVFGIYSLSVIEKCEKDLDKIAEKIIEIAKKSEYKRFKLEITRKDKSFPMTSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCH
DLKLALAPMVLKAVDNLIVDVHNPDLKIEVLVKKDGIQIFSKRIQGLKGLPVGVSGKGLS
HHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHH
LLSGGIDSPVASFLTMKRGMHVDFIHFMTPPHTSPEALDKVFELAKIVSKYNSKKFNLYV
HHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
CDFSMLLQELQHMPEESYKITIMRRMFMRIANKLAKQNGQEALITGESLGQVASQTIQSI
ECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHH
DVINSTSELPILRPVLTYDKEEIILISKFIKSYETSILPFDDACSLFVPKQPVTKPKKMA
HHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHH
EQQENAILNELLDYTMENKIQKFVFNGSFTEEINKED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCC
>Mature Secondary Structure
MKSILVRYGELTLKGNNKHMFISKLLDNIKYKLKKFDQSKIKFIKDNNSLVIDAEEEILE
CCCCEEEECEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHEEEEECCCCEEEECHHHHHH
DVLEELKTVFGIYSLSVIEKCEKDLDKIAEKIIEIAKKSEYKRFKLEITRKDKSFPMTSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCH
DLKLALAPMVLKAVDNLIVDVHNPDLKIEVLVKKDGIQIFSKRIQGLKGLPVGVSGKGLS
HHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHH
LLSGGIDSPVASFLTMKRGMHVDFIHFMTPPHTSPEALDKVFELAKIVSKYNSKKFNLYV
HHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
CDFSMLLQELQHMPEESYKITIMRRMFMRIANKLAKQNGQEALITGESLGQVASQTIQSI
ECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHH
DVINSTSELPILRPVLTYDKEEIILISKFIKSYETSILPFDDACSLFVPKQPVTKPKKMA
HHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHH
EQQENAILNELLDYTMENKIQKFVFNGSFTEEINKED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA