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The map label for this gene is polA [H]

Identifier: 50365399

GI number: 50365399

Start: 677164

End: 679851

Strand: Reverse

Name: polA [H]

Synonym: Mfl582

Alternate gene names: 50365399

Gene position: 679851-677164 (Counterclockwise)

Preceding gene: 50365400

Following gene: 50365398

Centisome position: 85.71

GC content: 25.07

Gene sequence:

>2688_bases
ATGAAGAAAATTTTATTAGTAGATGGAAACTCATTATTATTTAGAGCATATTATGCAAGTGCATACGCAGGACCAGTTTT
AAAAACAACAACCGGTATACCAACAAATGCAATTTTTTCTTTTGCGAATATGCTAACTAGTTTAATAAGTGATAGAAACT
ATTATGATGTAAAAGTAGCTTTTGATAAATCAAAGAAAACTTTCAGACATGATAAGTTAGAAAATTATAAAGCAGGAAGA
TCAGCAACTCCTGAAGACTTAATTCCCCAATTTCAAATTGTTAGAGAATTTTTAGATTCATCAAATATTTCTTGATGAGA
AAAAGAAAACTATGAAGCTGATGATTTAATTGGAACCATGTCAAAAATTATAGAAAATAATTTTAGTGAATTTGAAGTTG
AAATATTAACTAGTGATAAAGATATGTTTCAATTGATTAGTGATAAAACAAAAATATTATTACCAAAAACCGGAACATCA
AATCTAGAATTATTTGGAGAAGCAGAATTATTAGAAAAATGAGAAGTAGCTCCAAAAAATGTTGTTGATTTAAAAGGTCT
TATGGGTGATCCTTCAGATAATCTTAAAGGAGTTGAAGGTGTTGGTGAGAAAACTGCAATTAAATTATTAAAAGAATTCC
AAACTATAGAAGGAATATATGAAAATATTGATTCTATATCAGGTAAATTACAAGAAAAACTTATTAACGGAAAAGACTCA
GCAATTTTATGTAAAGAAATAGCAACAATAAATTGTGAAGTAGAAATAGAAAATTTGGTTTTTGAACCAATAAATTTAAA
TCTAAATGGACTTATTAATTTTTTAGAAAAATATGAAATGAATTCATTAGTAAAAAGATTATCTACTAGAGTTGGCAATA
TTAACGGAAAAGATGAACAAATTAACAAAGAAGAAATAAAGTTTAATCAATTAAAAAAATGAAATAATGAATTTTCTTGT
GAAACAAACTTTTTATATATTGAATCAATAGATGAAAATTATCATAAAGGACAAATATTAGGATTAGCTATTTGTAATGA
AAAAGGGTTCTTTTACTTAAATGACATAATAAATGATTTAGAGCTTGAACAATTTTTCTTAAATGATAATTTTAAAAAAA
ATACTTATGACATTAAGAAAACAGTAACTCTTTTAAGAAACAATAATATAAAATTTTCGTTTTCTAGTTTTACTTATGAT
GCAATGATTGCTTCTTATGTTTTAAATCCTAATGTAACTTCTAGAATTCAAAATTTAGCTAATATAGTAGAAACAGAATT
ATTTGTTGAGGAAGACGAACTTTTCTATGGTAAAGGTGTAAAACAAAATAAAGAAATAGATACTGCATCAAAAGCAAAAT
TTATTGTTGGAAAGTTAGAATTACTTTATTCAACACACAAACCAATAATAAATTTATTGAAAGAAAATAACCAATTTAAT
CTATACGAAAAAATCGAGCTTCCTTTGGTTGAAGTTTTATTTAATATGGAACAAAAAGGTATTTTAATTGATAAAGTTGA
ATTAGATAAGCAAACAGCAAGGACACTTTTTTTAATTGAAGAATTAGAAGAGAAAATGAAAGTTATATTAAAAGATCAAA
TAAGCGAAAATTTTAACTTTGGTTCGCCAAAACAAATTAAAGAATTATTATTTGATCAAATTGGTTTACCATCAAATAAA
AAACAAAGTACAGATAAAGAAGCTTTAGAAAAAATAATACATTTACACCCAGTTATAAATTTATTATTAGAACATAGAAA
ATTAAATAAACTATATTCAACATATCTTAGAGGTTTTGAAAAATTTATTTTTGATGATAATAAAGTTCACACTATATTTA
ATCAAACGCTTACTAATACAGGAAGATTGTCTTCATCTGAACCGAATATTCAAAATATATCTGTTAAGGATGAATTACAA
AGAGAAGCCCGTAAAATATTTATTTCTAATAATGATACAAAGTTTTTTAGTTTTGATTATTCTCAAATTGAACTTAGAGT
TTTGGCCCAGTTAGGACAAGAAGATACTTTATTAAATATTTTTAAGAATGATAGAGATGTTCATGCTGAAGCAGCTAGAA
AAATATTTAAATTAGAAGAAAATGAAGAAATTACAAGTGATCAAAGAAGAATAGCCAAAGTCTTTAACTTTGGAATTATA
TATGGTCTAAGTGACTTTGGGTTAAGTAATGACTTGAAAATATCAATTCCTGAAGCCAAAAGATTTATTGATGATTATTA
TCTTTCATTTCCAAAACTTATGACATATAAGAATAGTTTAATAAATCAAGCAACTGTTAATGGATATGCTGAAACTTACG
CAAACAGAAGAAGAATTGTTAATGAATTGGCGTCTTCGAATTTCATGGTAAAAAACTTTGGAAATAGAATAGCTGTAAAT
ATGCCAATTCAAGGGACTGCTGCTGATATATTAAAAGTAGCAATGAATAATATACATAAAGAATTTAGGGAAAATAATCT
TAAAAGTTTTATGACTGCACAAATTCATGATGAAATCATTTTTGAAATTTTTGAAAGCGAAGAATCACTAGCAGTCAAAA
TAGTTGAAGAATGCATGCAAAATGCTTTTATTGATTTATCAAAAATAGTTCAAAAAGAAGAACAACCAATAGAAGTTAAA
CTTGAAGTTAACAAGTCAAAAGGTAATAATTGGTTTGAGTTAAAATAA

Upstream 100 bases:

>100_bases
AAAAATTACGTTTTAAAAATAAGAACGCAAAAATATAAGAACAAAATAACAGGAATACTTGAATCTATAGTTAAACCGGT
TTAATTGAAAAGAGGGAATT

Downstream 100 bases:

>100_bases
TTTAAGGAGTGGGAAAAATGCCAGAACTACCAGAGGTTAGAACGGTTGCTGTTTTTTTAAATAAAAGAATAGTCAATACA
ACAATTTTAAAAGCAGAATG

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 895; Mature: 895

Protein sequence:

>895_residues
MKKILLVDGNSLLFRAYYASAYAGPVLKTTTGIPTNAIFSFANMLTSLISDRNYYDVKVAFDKSKKTFRHDKLENYKAGR
SATPEDLIPQFQIVREFLDSSNISWWEKENYEADDLIGTMSKIIENNFSEFEVEILTSDKDMFQLISDKTKILLPKTGTS
NLELFGEAELLEKWEVAPKNVVDLKGLMGDPSDNLKGVEGVGEKTAIKLLKEFQTIEGIYENIDSISGKLQEKLINGKDS
AILCKEIATINCEVEIENLVFEPINLNLNGLINFLEKYEMNSLVKRLSTRVGNINGKDEQINKEEIKFNQLKKWNNEFSC
ETNFLYIESIDENYHKGQILGLAICNEKGFFYLNDIINDLELEQFFLNDNFKKNTYDIKKTVTLLRNNNIKFSFSSFTYD
AMIASYVLNPNVTSRIQNLANIVETELFVEEDELFYGKGVKQNKEIDTASKAKFIVGKLELLYSTHKPIINLLKENNQFN
LYEKIELPLVEVLFNMEQKGILIDKVELDKQTARTLFLIEELEEKMKVILKDQISENFNFGSPKQIKELLFDQIGLPSNK
KQSTDKEALEKIIHLHPVINLLLEHRKLNKLYSTYLRGFEKFIFDDNKVHTIFNQTLTNTGRLSSSEPNIQNISVKDELQ
REARKIFISNNDTKFFSFDYSQIELRVLAQLGQEDTLLNIFKNDRDVHAEAARKIFKLEENEEITSDQRRIAKVFNFGII
YGLSDFGLSNDLKISIPEAKRFIDDYYLSFPKLMTYKNSLINQATVNGYAETYANRRRIVNELASSNFMVKNFGNRIAVN
MPIQGTAADILKVAMNNIHKEFRENNLKSFMTAQIHDEIIFEIFESEESLAVKIVEECMQNAFIDLSKIVQKEEQPIEVK
LEVNKSKGNNWFELK

Sequences:

>Translated_895_residues
MKKILLVDGNSLLFRAYYASAYAGPVLKTTTGIPTNAIFSFANMLTSLISDRNYYDVKVAFDKSKKTFRHDKLENYKAGR
SATPEDLIPQFQIVREFLDSSNIS**EKENYEADDLIGTMSKIIENNFSEFEVEILTSDKDMFQLISDKTKILLPKTGTS
NLELFGEAELLEK*EVAPKNVVDLKGLMGDPSDNLKGVEGVGEKTAIKLLKEFQTIEGIYENIDSISGKLQEKLINGKDS
AILCKEIATINCEVEIENLVFEPINLNLNGLINFLEKYEMNSLVKRLSTRVGNINGKDEQINKEEIKFNQLKK*NNEFSC
ETNFLYIESIDENYHKGQILGLAICNEKGFFYLNDIINDLELEQFFLNDNFKKNTYDIKKTVTLLRNNNIKFSFSSFTYD
AMIASYVLNPNVTSRIQNLANIVETELFVEEDELFYGKGVKQNKEIDTASKAKFIVGKLELLYSTHKPIINLLKENNQFN
LYEKIELPLVEVLFNMEQKGILIDKVELDKQTARTLFLIEELEEKMKVILKDQISENFNFGSPKQIKELLFDQIGLPSNK
KQSTDKEALEKIIHLHPVINLLLEHRKLNKLYSTYLRGFEKFIFDDNKVHTIFNQTLTNTGRLSSSEPNIQNISVKDELQ
REARKIFISNNDTKFFSFDYSQIELRVLAQLGQEDTLLNIFKNDRDVHAEAARKIFKLEENEEITSDQRRIAKVFNFGII
YGLSDFGLSNDLKISIPEAKRFIDDYYLSFPKLMTYKNSLINQATVNGYAETYANRRRIVNELASSNFMVKNFGNRIAVN
MPIQGTAADILKVAMNNIHKEFRENNLKSFMTAQIHDEIIFEIFESEESLAVKIVEECMQNAFIDLSKIVQKEEQPIEVK
LEVNKSKGNNWFELK
>Mature_895_residues
MKKILLVDGNSLLFRAYYASAYAGPVLKTTTGIPTNAIFSFANMLTSLISDRNYYDVKVAFDKSKKTFRHDKLENYKAGR
SATPEDLIPQFQIVREFLDSSNIS**EKENYEADDLIGTMSKIIENNFSEFEVEILTSDKDMFQLISDKTKILLPKTGTS
NLELFGEAELLEK*EVAPKNVVDLKGLMGDPSDNLKGVEGVGEKTAIKLLKEFQTIEGIYENIDSISGKLQEKLINGKDS
AILCKEIATINCEVEIENLVFEPINLNLNGLINFLEKYEMNSLVKRLSTRVGNINGKDEQINKEEIKFNQLKK*NNEFSC
ETNFLYIESIDENYHKGQILGLAICNEKGFFYLNDIINDLELEQFFLNDNFKKNTYDIKKTVTLLRNNNIKFSFSSFTYD
AMIASYVLNPNVTSRIQNLANIVETELFVEEDELFYGKGVKQNKEIDTASKAKFIVGKLELLYSTHKPIINLLKENNQFN
LYEKIELPLVEVLFNMEQKGILIDKVELDKQTARTLFLIEELEEKMKVILKDQISENFNFGSPKQIKELLFDQIGLPSNK
KQSTDKEALEKIIHLHPVINLLLEHRKLNKLYSTYLRGFEKFIFDDNKVHTIFNQTLTNTGRLSSSEPNIQNISVKDELQ
REARKIFISNNDTKFFSFDYSQIELRVLAQLGQEDTLLNIFKNDRDVHAEAARKIFKLEENEEITSDQRRIAKVFNFGII
YGLSDFGLSNDLKISIPEAKRFIDDYYLSFPKLMTYKNSLINQATVNGYAETYANRRRIVNELASSNFMVKNFGNRIAVN
MPIQGTAADILKVAMNNIHKEFRENNLKSFMTAQIHDEIIFEIFESEESLAVKIVEECMQNAFIDLSKIVQKEEQPIEVK
LEVNKSKGNNWFELK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=466, Percent_Identity=28.3261802575107, Blast_Score=186, Evalue=1e-46,
Organism=Homo sapiens, GI139394648, Length=621, Percent_Identity=23.3494363929147, Blast_Score=139, Evalue=2e-32,
Organism=Escherichia coli, GI1790294, Length=953, Percent_Identity=31.5844700944386, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI226510970, Length=200, Percent_Identity=32.5, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI86563272, Length=422, Percent_Identity=28.1990521327014, Blast_Score=159, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17933644, Length=546, Percent_Identity=27.2893772893773, Blast_Score=171, Evalue=3e-42,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 102432; Mature: 102432

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLVDGNSLLFRAYYASAYAGPVLKTTTGIPTNAIFSFANMLTSLISDRNYYDVKVA
CCEEEEECCCCEEEHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
FDKSKKTFRHDKLENYKAGRSATPEDLIPQFQIVREFLDSSNISEKENYEADDLIGTMSK
ECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
IIENNFSEFEVEILTSDKDMFQLISDKTKILLPKTGTSNLELFGEAELLEKEVAPKNVVD
HHHCCCCCEEEEEEECCHHHHHHHCCCCEEEEECCCCCCCEEECCHHHHHHHCCCCCHHH
LKGLMGDPSDNLKGVEGVGEKTAIKLLKEFQTIEGIYENIDSISGKLQEKLINGKDSAIL
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
CKEIATINCEVEIENLVFEPINLNLNGLINFLEKYEMNSLVKRLSTRVGNINGKDEQINK
EEHHHEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
EEIKFNQLKKNNEFSCETNFLYIESIDENYHKGQILGLAICNEKGFFYLNDIINDLELEQ
HHHHHHHHCCCCCCCEEECEEEEEECCCCCCCCEEEEEEEECCCCCEEHHHHHCHHHHHH
FFLNDNFKKNTYDIKKTVTLLRNNNIKFSFSSFTYDAMIASYVLNPNVTSRIQNLANIVE
HHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
TELFVEEDELFYGKGVKQNKEIDTASKAKFIVGKLELLYSTHKPIINLLKENNQFNLYEK
HHHEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IELPLVEVLFNMEQKGILIDKVELDKQTARTLFLIEELEEKMKVILKDQISENFNFGSPK
HCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QIKELLFDQIGLPSNKKQSTDKEALEKIIHLHPVINLLLEHRKLNKLYSTYLRGFEKFIF
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDNKVHTIFNQTLTNTGRLSSSEPNIQNISVKDELQREARKIFISNNDTKFFSFDYSQIE
CCCCEEHHHHHHHCCCCCCCCCCCCCEECCHHHHHHHHHHEEEEECCCCEEEEECHHHHH
LRVLAQLGQEDTLLNIFKNDRDVHAEAARKIFKLEENEEITSDQRRIAKVFNFGIIYGLS
HHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECH
DFGLSNDLKISIPEAKRFIDDYYLSFPKLMTYKNSLINQATVNGYAETYANRRRIVNELA
HCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSNFMVKNFGNRIAVNMPIQGTAADILKVAMNNIHKEFRENNLKSFMTAQIHDEIIFEIF
HCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ESEESLAVKIVEECMQNAFIDLSKIVQKEEQPIEVKLEVNKSKGNNWFELK
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEC
>Mature Secondary Structure
MKKILLVDGNSLLFRAYYASAYAGPVLKTTTGIPTNAIFSFANMLTSLISDRNYYDVKVA
CCEEEEECCCCEEEHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
FDKSKKTFRHDKLENYKAGRSATPEDLIPQFQIVREFLDSSNISEKENYEADDLIGTMSK
ECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
IIENNFSEFEVEILTSDKDMFQLISDKTKILLPKTGTSNLELFGEAELLEKEVAPKNVVD
HHHCCCCCEEEEEEECCHHHHHHHCCCCEEEEECCCCCCCEEECCHHHHHHHCCCCCHHH
LKGLMGDPSDNLKGVEGVGEKTAIKLLKEFQTIEGIYENIDSISGKLQEKLINGKDSAIL
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
CKEIATINCEVEIENLVFEPINLNLNGLINFLEKYEMNSLVKRLSTRVGNINGKDEQINK
EEHHHEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
EEIKFNQLKKNNEFSCETNFLYIESIDENYHKGQILGLAICNEKGFFYLNDIINDLELEQ
HHHHHHHHCCCCCCCEEECEEEEEECCCCCCCCEEEEEEEECCCCCEEHHHHHCHHHHHH
FFLNDNFKKNTYDIKKTVTLLRNNNIKFSFSSFTYDAMIASYVLNPNVTSRIQNLANIVE
HHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
TELFVEEDELFYGKGVKQNKEIDTASKAKFIVGKLELLYSTHKPIINLLKENNQFNLYEK
HHHEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IELPLVEVLFNMEQKGILIDKVELDKQTARTLFLIEELEEKMKVILKDQISENFNFGSPK
HCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QIKELLFDQIGLPSNKKQSTDKEALEKIIHLHPVINLLLEHRKLNKLYSTYLRGFEKFIF
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDNKVHTIFNQTLTNTGRLSSSEPNIQNISVKDELQREARKIFISNNDTKFFSFDYSQIE
CCCCEEHHHHHHHCCCCCCCCCCCCCEECCHHHHHHHHHHEEEEECCCCEEEEECHHHHH
LRVLAQLGQEDTLLNIFKNDRDVHAEAARKIFKLEENEEITSDQRRIAKVFNFGIIYGLS
HHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECH
DFGLSNDLKISIPEAKRFIDDYYLSFPKLMTYKNSLINQATVNGYAETYANRRRIVNELA
HCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSNFMVKNFGNRIAVNMPIQGTAADILKVAMNNIHKEFRENNLKSFMTAQIHDEIIFEIF
HCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ESEESLAVKIVEECMQNAFIDLSKIVQKEEQPIEVKLEVNKSKGNNWFELK
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]