Click here to switch to the map view.

The map label for this gene is mutM [H]

Identifier: 50365398

GI number: 50365398

Start: 676319

End: 677146

Strand: Reverse

Name: mutM [H]

Synonym: Mfl581

Alternate gene names: 50365398

Gene position: 677146-676319 (Counterclockwise)

Preceding gene: 50365399

Following gene: 50365397

Centisome position: 85.37

GC content: 26.45

Gene sequence:

>828_bases
ATGCCAGAACTACCAGAGGTTAGAACGGTTGCTGTTTTTTTAAATAAAAGAATAGTCAATACAACAATTTTAAAAGCAGA
ATGTTTTTTTGAAAAAATGATTTGAAGAAATGAAGTTAAAGACTTTTATAAAAGTGTTTTAAATCAAAAAATATTGAAAG
TTTCAAATTATGGAAAATATTTAATGTTTGAACTTTCAAATCAAATTATTATTAGTCATTTGAGAATGGAAGGCAAATGA
AGCATTTCAAAAAAAGAAATAGATATTTATAATGCAAACCATTTAAGATTGCAATTTGAGCTATCAAATGGTGAATATTT
AAAATATTATGATTCTAGAAAATTTGGAACAATTGAAATTTGAGATAAAAAAAATTATAAACAAAAAAGTGGAATGGATA
AATTAGGTCCAGAGCCATTAAATAGTCAACCTTCTTTTGAATATCTAAAAGAAAAAGCAATTAAATCTAATTCCTTAATT
AAGGCTTTTATATTAGATCAAAGTGTTCTTTGTGGAATTGGTAATATATATGCAAACGAAATTTTATTTGCTGCAGGTAT
TAATCCAGAAAGAATAACAAAAACATTAACAGATGAAGAACTTAAAAAAATTATCATGTTTTCTAATGCAATATTAGAAA
AAGCTATCAGTTTAAAGGGGACTTCAATTCATAGTTATAAATCTGGGGATGGCGAAACTGGTCAATTCCAACATGAATTA
AAAGTGCACTTAAGAAAAGACGAAAAATGCTTTGTTTGCGGAACAAAGATATTAAAAAAACAAGTTGCAGGTCGCGGAAC
ATACTTTTGTGCAAAATGTCAACACTAG

Upstream 100 bases:

>100_bases
TAGTTCAAAAAGAAGAACAACCAATAGAAGTTAAACTTGAAGTTAACAAGTCAAAAGGTAATAATTGGTTTGAGTTAAAA
TAATTTAAGGAGTGGGAAAA

Downstream 100 bases:

>100_bases
CTTTTTGTGGAAATGTGGAAAACACAAAATAAATCTATGTTTAACATAGGTTTTTTTTAATGTTTATAAACACAACAAAA
AATAATTAAAAAATCTATAT

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPELPEVRTVAVFLNKRIVNTTILKAECFFEKMIWRNEVKDFYKSVLNQKILKVSNYGKYLMFELSNQIIISHLRMEGKW
SISKKEIDIYNANHLRLQFELSNGEYLKYYDSRKFGTIEIWDKKNYKQKSGMDKLGPEPLNSQPSFEYLKEKAIKSNSLI
KAFILDQSVLCGIGNIYANEILFAAGINPERITKTLTDEELKKIIMFSNAILEKAISLKGTSIHSYKSGDGETGQFQHEL
KVHLRKDEKCFVCGTKILKKQVAGRGTYFCAKCQH

Sequences:

>Translated_275_residues
MPELPEVRTVAVFLNKRIVNTTILKAECFFEKMI*RNEVKDFYKSVLNQKILKVSNYGKYLMFELSNQIIISHLRMEGK*
SISKKEIDIYNANHLRLQFELSNGEYLKYYDSRKFGTIEI*DKKNYKQKSGMDKLGPEPLNSQPSFEYLKEKAIKSNSLI
KAFILDQSVLCGIGNIYANEILFAAGINPERITKTLTDEELKKIIMFSNAILEKAISLKGTSIHSYKSGDGETGQFQHEL
KVHLRKDEKCFVCGTKILKKQVAGRGTYFCAKCQH
>Mature_274_residues
PELPEVRTVAVFLNKRIVNTTILKAECFFEKMI*RNEVKDFYKSVLNQKILKVSNYGKYLMFELSNQIIISHLRMEGK*S
ISKKEIDIYNANHLRLQFELSNGEYLKYYDSRKFGTIEI*DKKNYKQKSGMDKLGPEPLNSQPSFEYLKEKAIKSNSLIK
AFILDQSVLCGIGNIYANEILFAAGINPERITKTLTDEELKKIIMFSNAILEKAISLKGTSIHSYKSGDGETGQFQHELK
VHLRKDEKCFVCGTKILKKQVAGRGTYFCAKCQH

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1790066, Length=272, Percent_Identity=37.1323529411765, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1786932, Length=284, Percent_Identity=25.3521126760563, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS [H]

EC number: =3.2.2.23; =4.2.99.18 [H]

Molecular weight: Translated: 31177; Mature: 31046

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS01242 ZF_FPG_1 ; PS51066 ZF_FPG_2 ; PS51068 FPG_CAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVRTVAVFLNKRIVNTTILKAECFFEKMIRNEVKDFYKSVLNQKILKVSNYGKYL
CCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
MFELSNQIIISHLRMEGKSISKKEIDIYNANHLRLQFELSNGEYLKYYDSRKFGTIEIDK
EEEECCHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCEEEEECCCCCEEEEECH
KNYKQKSGMDKLGPEPLNSQPSFEYLKEKAIKSNSLIKAFILDQSVLCGIGNIYANEILF
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHEEE
AAGINPERITKTLTDEELKKIIMFSNAILEKAISLKGTSIHSYKSGDGETGQFQHELKVH
EECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEEE
LRKDEKCFVCGTKILKKQVAGRGTYFCAKCQH
ECCCCEEEEHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure 
PELPEVRTVAVFLNKRIVNTTILKAECFFEKMIRNEVKDFYKSVLNQKILKVSNYGKYL
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
MFELSNQIIISHLRMEGKSISKKEIDIYNANHLRLQFELSNGEYLKYYDSRKFGTIEIDK
EEEECCHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCEEEEECCCCCEEEEECH
KNYKQKSGMDKLGPEPLNSQPSFEYLKEKAIKSNSLIKAFILDQSVLCGIGNIYANEILF
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHEEE
AAGINPERITKTLTDEELKKIIMFSNAILEKAISLKGTSIHSYKSGDGETGQFQHELKVH
EECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEEE
LRKDEKCFVCGTKILKKQVAGRGTYFCAKCQH
ECCCCEEEEHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]