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The map label for this gene is scrB [H]

Identifier: 50365343

GI number: 50365343

Start: 618620

End: 620059

Strand: Reverse

Name: scrB [H]

Synonym: Mfl526

Alternate gene names: 50365343

Gene position: 620059-618620 (Counterclockwise)

Preceding gene: 50365344

Following gene: 50365342

Centisome position: 78.17

GC content: 25.76

Gene sequence:

>1440_bases
ATGAAATTAAATAAAGAAACAATATTAAATTTAATAACAGAACATAATCAAGATGATATTAAAAAAGCAAATGAATTAGT
AGCAAGTGATAAATATTATAGACCAACATATCATATAGCACCTCCAAATGGATTATTGAATGATCCAAATGGATTAGTTT
ATATAGATGGAGAACATTATATTCATTATCAATGATCACCACTTCAACCATATCATGGGATGAAACATTGACGTTTAGTT
AAAACTAAAGATTTTGTTAATTATGATGATTTAGGTGTATCAGTAATACCAACAGAAGAATTCGAAAGAACAGGAGCATT
TTCTGGATCAGCATTTAAAGAAAAAGACGGAGTAAAAATTTACTACACAGGTAATATTGAAGAAAATGGCGAAATGATTG
AAGAAGTGCAAATCAAAGCAGACTTCATTGATGAAAAAATTGTAAATAAAAGAGTTGTTGTAGAGCACGATAAAAATCTT
TTCACACCTCACGTAAGAGATCCAAAAATATTTGAATATGAAAATAACAAATATATGATTTTTGGTGCTCAGTGTAAAGC
TGATATGCTTGGTGGGTTAGTTTTCTACAAAACTGATGATATGGAAAAATATACTTTTGATAGAATACTAAAACCATCAT
TAGACCAAACCTATGGATACATGTGAGAATGCCCTAATTTAGATTATCTAGAAGGAAAAATGCTATTTATGCAATCATCA
GAAGGGTGATTTAATGAAGAAAATCCTTATGAACTAAATAGCTCAAGAAATGTTGTTTATACTCAAATAGATAAATTAGA
TTTTGAAAACAATAAATTGAATGAAAAATCAATTTTAAAAACAATGGATTTCGGACATGATTTTTATGCACCGCAAACTT
ATTGAGTTGATAAAAAACTTTTAATGATAGGTTGATTAGGTGCGGTTGATGTTCAATATCCAACAGATGAATACTCATGA
CACTCAATGTTAACAATACCAAGAGAACTAAGCTGATCAAAAGAAATTTTAGTTCAAAAACCTTACTCAGAATTCAAAAA
AAATGTTTTAACTAAAACAACTAAAAAAGCATTTAAAGAATTAAGTGTAAATAAAGCAATACATTTAGAGTTTGATTTAA
GCGATAATTTAGACATTAAAATTCTTAACGAAAAAAATGAATTTATAGAAATAAAATTTACTGATAAAGAAATAATTTTA
GATAGAACAAATCAAACTGCAAAAGTTGATTGAAATTTTGAAACACCAAGAAAAGCATTAAGAAAATTCAAAAATCAAAA
AGTTGAAATATTTATTGATTCATCATCAATTGAAATATTTGCGGATAATTATGAAACAATTTTTACTTCAAGATTTTTTG
TAAAAAACTTTAATAAAATTCAATTAAATAAAGAACATGAAATGTTACTATCTGATATTAAAGAAATGAAATTAAAATAA

Upstream 100 bases:

>100_bases
TTTATAACAACTTTCTTTGCAACAATTGGTTTATCAAAAATTAAAGTTTTCAAAGAAATGAATGCAAAAGTTTTAGAAAG
AGATTTTTCGGTGAATTAAC

Downstream 100 bases:

>100_bases
TAAATAATTAAGCAACTTTTAGGTTGCTTTTTATAATAAAAATTTTAATTTCTTTAATTACAAGCAACTGTGCTATAATA
TATAAATGACTTGTGATTTA

Product: sucrose-6-phosphate hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKLNKETILNLITEHNQDDIKKANELVASDKYYRPTYHIAPPNGLLNDPNGLVYIDGEHYIHYQWSPLQPYHGMKHWRLV
KTKDFVNYDDLGVSVIPTEEFERTGAFSGSAFKEKDGVKIYYTGNIEENGEMIEEVQIKADFIDEKIVNKRVVVEHDKNL
FTPHVRDPKIFEYENNKYMIFGAQCKADMLGGLVFYKTDDMEKYTFDRILKPSLDQTYGYMWECPNLDYLEGKMLFMQSS
EGWFNEENPYELNSSRNVVYTQIDKLDFENNKLNEKSILKTMDFGHDFYAPQTYWVDKKLLMIGWLGAVDVQYPTDEYSW
HSMLTIPRELSWSKEILVQKPYSEFKKNVLTKTTKKAFKELSVNKAIHLEFDLSDNLDIKILNEKNEFIEIKFTDKEIIL
DRTNQTAKVDWNFETPRKALRKFKNQKVEIFIDSSSIEIFADNYETIFTSRFFVKNFNKIQLNKEHEMLLSDIKEMKLK

Sequences:

>Translated_479_residues
MKLNKETILNLITEHNQDDIKKANELVASDKYYRPTYHIAPPNGLLNDPNGLVYIDGEHYIHYQ*SPLQPYHGMKH*RLV
KTKDFVNYDDLGVSVIPTEEFERTGAFSGSAFKEKDGVKIYYTGNIEENGEMIEEVQIKADFIDEKIVNKRVVVEHDKNL
FTPHVRDPKIFEYENNKYMIFGAQCKADMLGGLVFYKTDDMEKYTFDRILKPSLDQTYGYM*ECPNLDYLEGKMLFMQSS
EG*FNEENPYELNSSRNVVYTQIDKLDFENNKLNEKSILKTMDFGHDFYAPQTY*VDKKLLMIG*LGAVDVQYPTDEYS*
HSMLTIPRELS*SKEILVQKPYSEFKKNVLTKTTKKAFKELSVNKAIHLEFDLSDNLDIKILNEKNEFIEIKFTDKEIIL
DRTNQTAKVD*NFETPRKALRKFKNQKVEIFIDSSSIEIFADNYETIFTSRFFVKNFNKIQLNKEHEMLLSDIKEMKLK
>Mature_479_residues
MKLNKETILNLITEHNQDDIKKANELVASDKYYRPTYHIAPPNGLLNDPNGLVYIDGEHYIHYQ*SPLQPYHGMKH*RLV
KTKDFVNYDDLGVSVIPTEEFERTGAFSGSAFKEKDGVKIYYTGNIEENGEMIEEVQIKADFIDEKIVNKRVVVEHDKNL
FTPHVRDPKIFEYENNKYMIFGAQCKADMLGGLVFYKTDDMEKYTFDRILKPSLDQTYGYM*ECPNLDYLEGKMLFMQSS
EG*FNEENPYELNSSRNVVYTQIDKLDFENNKLNEKSILKTMDFGHDFYAPQTY*VDKKLLMIG*LGAVDVQYPTDEYS*
HSMLTIPRELS*SKEILVQKPYSEFKKNVLTKTTKKAFKELSVNKAIHLEFDLSDNLDIKILNEKNEFIEIKFTDKEIIL
DRTNQTAKVD*NFETPRKALRKFKNQKVEIFIDSSSIEIFADNYETIFTSRFFVKNFNKIQLNKEHEMLLSDIKEMKLK

Specific function: Enables the bacterium to metabolize sucrose as a sole carbon source [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 54835; Mature: 54835

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNKETILNLITEHNQDDIKKANELVASDKYYRPTYHIAPPNGLLNDPNGLVYIDGEHY
CCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEECCCEE
IHYQSPLQPYHGMKHRLVKTKDFVNYDDLGVSVIPTEEFERTGAFSGSAFKEKDGVKIYY
EEECCCCCCCCCCHHHHCCHHCCCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCEEEEE
TGNIEENGEMIEEVQIKADFIDEKIVNKRVVVEHDKNLFTPHVRDPKIFEYENNKYMIFG
ECCCCCCCHHHHHEEEHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCEEEEEE
AQCKADMLGGLVFYKTDDMEKYTFDRILKPSLDQTYGYMECPNLDYLEGKMLFMQSSEGF
CCCCHHHHCCEEEEECCCCCHHHHHHHCCCCHHHCCCEEECCCCCCCCCEEEEEECCCCC
NEENPYELNSSRNVVYTQIDKLDFENNKLNEKSILKTMDFGHDFYAPQTYVDKKLLMIGL
CCCCCEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHCCEEEEEEC
GAVDVQYPTDEYSHSMLTIPRELSSKEILVQKPYSEFKKNVLTKTTKKAFKELSVNKAIH
CEEEEECCCCCCCCCEEEECHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEE
LEFDLSDNLDIKILNEKNEFIEIKFTDKEIILDRTNQTAKVDNFETPRKALRKFKNQKVE
EEEECCCCCEEEEEECCCCEEEEEECCCEEEEECCCCCEECCCCCCHHHHHHHHCCCEEE
IFIDSSSIEIFADNYETIFTSRFFVKNFNKIQLNKEHEMLLSDIKEMKLK
EEEECCCEEEEECCCEEEEEEEHHHCCCCEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLNKETILNLITEHNQDDIKKANELVASDKYYRPTYHIAPPNGLLNDPNGLVYIDGEHY
CCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEECCCEE
IHYQSPLQPYHGMKHRLVKTKDFVNYDDLGVSVIPTEEFERTGAFSGSAFKEKDGVKIYY
EEECCCCCCCCCCHHHHCCHHCCCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCEEEEE
TGNIEENGEMIEEVQIKADFIDEKIVNKRVVVEHDKNLFTPHVRDPKIFEYENNKYMIFG
ECCCCCCCHHHHHEEEHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCEEEEEE
AQCKADMLGGLVFYKTDDMEKYTFDRILKPSLDQTYGYMECPNLDYLEGKMLFMQSSEGF
CCCCHHHHCCEEEEECCCCCHHHHHHHCCCCHHHCCCEEECCCCCCCCCEEEEEECCCCC
NEENPYELNSSRNVVYTQIDKLDFENNKLNEKSILKTMDFGHDFYAPQTYVDKKLLMIGL
CCCCCEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHCCEEEEEEC
GAVDVQYPTDEYSHSMLTIPRELSSKEILVQKPYSEFKKNVLTKTTKKAFKELSVNKAIH
CEEEEECCCCCCCCCEEEECHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEE
LEFDLSDNLDIKILNEKNEFIEIKFTDKEIILDRTNQTAKVDNFETPRKALRKFKNQKVE
EEEECCCCCEEEEEECCCCEEEEEECCCEEEEECCCCCEECCCCCCHHHHHHHHCCCEEE
IFIDSSSIEIFADNYETIFTSRFFVKNFNKIQLNKEHEMLLSDIKEMKLK
EEEECCCEEEEECCCEEEEEEEHHHCCCCEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2551785 [H]