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The map label for this gene is amiD [H]

Identifier: 50364910

GI number: 50364910

Start: 116087

End: 117058

Strand: Direct

Name: amiD [H]

Synonym: Mfl095

Alternate gene names: 50364910

Gene position: 116087-117058 (Clockwise)

Preceding gene: 50364909

Following gene: 50364911

Centisome position: 14.63

GC content: 29.94

Gene sequence:

>972_bases
ATGATAAACATCGATAAAGAAAAATTTGAACGCGAAAATAAAGTTAATTTTGAAGAAATTGACGAAAAAATGTTTGAAGT
AGTCGAAGAACAAACTAGCGAAAGCGAAAGATTAAATTCTAAACCATATAGCTACTGAAAGTCTGTTGGTAAATTATTAG
TTACATCTCCTACTTTCATGATTTCTATTTTAGTATTAATAACTGTTTTACTTTTAGCATTCATTGTTCCAGAAGTAATG
AACTATAAAAATACTTCAAGTACTATTGGAGATCCTGCATTGCCATCTTGAGATCACATATTTGGTATTGGGATGAATGG
TGAAGATTTATTTGCTAGAGTTTGAGCAGGAACAAGAACAACTTTACTATTTGCTTTCTTAATTGCTGCTATTCAAGTTG
CAGTTGGGGTTATATTAGGTTCAATTTGAGGTTACTTTAGAAAATCAGATTTAATCTTCATTCAAGTTGCAAGTATTATT
ACAATTGTTCCTCAATTCATTTTATTACTATTAATGGTTTTCTTATTCAATAGTAAAGGTTACTGAGTTATTGTTTTTGC
TATATCACTTCAAGCATGAGTTGGTATTGCTCAAATGGTAAGGGTACAAATTATGTTAGTTAAAAATACTGACTATAATA
CTGCATCAATTAGTTTAGGTTCAAGTTCATACAGAATCATAAATAAAAATATTATGCCAAAAATTTTACCAGTTATTGTT
CAAACAGCGGCATTTGCAATTCCGACTGCTATTTCAATTGAAGCATCACTTGCATACTTAGCATTTGACTTTATTCCAGC
AGGTCAAACTTCTTTAGGGCAAATTTTAAACCAAGTTATGAATGAAACAAAATGACAAATTTATCCAAACTTATTAATTG
CTCCAATGGCAGTTATTATGATAGTTTCAGTTGTATTCTTCTTAGCTGCTAGAGTATTTGCTGACTCATTAGATCCGAAA
AATCATAGATAG

Upstream 100 bases:

>100_bases
ATCATTAGTAAAAATTGCAGCAACATTAGTTTCTGATTTATTCATGGTATTAATGGATCCAAGAGTTAAATTAAGTGGTA
GATAGAAAGAGGGGAAAAAT

Downstream 100 bases:

>100_bases
GAGACCAACTTATGGCTACAAATAAAGATAAAATTATTTCTCTTCAAGATGTTGTTGTTAAATTCAATGTTCGTGGAAGA
ATGTTAACAGCTATTAGAAA

Product: oligopeptide ABC transporter permease component

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MINIDKEKFERENKVNFEEIDEKMFEVVEEQTSESERLNSKPYSYWKSVGKLLVTSPTFMISILVLITVLLLAFIVPEVM
NYKNTSSTIGDPALPSWDHIFGIGMNGEDLFARVWAGTRTTLLFAFLIAAIQVAVGVILGSIWGYFRKSDLIFIQVASII
TIVPQFILLLLMVFLFNSKGYWVIVFAISLQAWVGIAQMVRVQIMLVKNTDYNTASISLGSSSYRIINKNIMPKILPVIV
QTAAFAIPTAISIEASLAYLAFDFIPAGQTSLGQILNQVMNETKWQIYPNLLIAPMAVIMIVSVVFFLAARVFADSLDPK
NHR

Sequences:

>Translated_323_residues
MINIDKEKFERENKVNFEEIDEKMFEVVEEQTSESERLNSKPYSY*KSVGKLLVTSPTFMISILVLITVLLLAFIVPEVM
NYKNTSSTIGDPALPS*DHIFGIGMNGEDLFARV*AGTRTTLLFAFLIAAIQVAVGVILGSI*GYFRKSDLIFIQVASII
TIVPQFILLLLMVFLFNSKGY*VIVFAISLQA*VGIAQMVRVQIMLVKNTDYNTASISLGSSSYRIINKNIMPKILPVIV
QTAAFAIPTAISIEASLAYLAFDFIPAGQTSLGQILNQVMNETK*QIYPNLLIAPMAVIMIVSVVFFLAARVFADSLDPK
NHR
>Mature_323_residues
MINIDKEKFERENKVNFEEIDEKMFEVVEEQTSESERLNSKPYSY*KSVGKLLVTSPTFMISILVLITVLLLAFIVPEVM
NYKNTSSTIGDPALPS*DHIFGIGMNGEDLFARV*AGTRTTLLFAFLIAAIQVAVGVILGSI*GYFRKSDLIFIQVASII
TIVPQFILLLLMVFLFNSKGY*VIVFAISLQA*VGIAQMVRVQIMLVKNTDYNTASISLGSSSYRIINKNIMPKILPVIV
QTAAFAIPTAISIEASLAYLAFDFIPAGQTSLGQILNQVMNETK*QIYPNLLIAPMAVIMIVSVVFFLAARVFADSLDPK
NHR

Specific function: Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788505, Length=230, Percent_Identity=27.3913043478261, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787760, Length=295, Percent_Identity=22.7118644067797, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787054, Length=257, Percent_Identity=24.9027237354086, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787498, Length=290, Percent_Identity=26.551724137931, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34792; Mature: 34792

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINIDKEKFERENKVNFEEIDEKMFEVVEEQTSESERLNSKPYSYKSVGKLLVTSPTFMI
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHH
SILVLITVLLLAFIVPEVMNYKNTSSTIGDPALPSDHIFGIGMNGEDLFARVAGTRTTLL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
FAFLIAAIQVAVGVILGSIGYFRKSDLIFIQVASIITIVPQFILLLLMVFLFNSKGYVIV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
FAISLQAVGIAQMVRVQIMLVKNTDYNTASISLGSSSYRIINKNIMPKILPVIVQTAAFA
EEEHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHH
IPTAISIEASLAYLAFDFIPAGQTSLGQILNQVMNETKQIYPNLLIAPMAVIMIVSVVFF
HCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LAARVFADSLDPKNHR
HHHHHHHHCCCCCCCC
>Mature Secondary Structure
MINIDKEKFERENKVNFEEIDEKMFEVVEEQTSESERLNSKPYSYKSVGKLLVTSPTFMI
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHH
SILVLITVLLLAFIVPEVMNYKNTSSTIGDPALPSDHIFGIGMNGEDLFARVAGTRTTLL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
FAFLIAAIQVAVGVILGSIGYFRKSDLIFIQVASIITIVPQFILLLLMVFLFNSKGYVIV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
FAISLQAVGIAQMVRVQIMLVKNTDYNTASISLGSSSYRIINKNIMPKILPVIVQTAAFA
EEEHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHH
IPTAISIEASLAYLAFDFIPAGQTSLGQILNQVMNETKQIYPNLLIAPMAVIMIVSVVFF
HCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LAARVFADSLDPKNHR
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2352474; 11463916 [H]