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The map label for this gene is trxB [H]

Identifier: 50364879

GI number: 50364879

Start: 82747

End: 83682

Strand: Direct

Name: trxB [H]

Synonym: Mfl064

Alternate gene names: 50364879

Gene position: 82747-83682 (Clockwise)

Preceding gene: 50364878

Following gene: 50364880

Centisome position: 10.43

GC content: 31.62

Gene sequence:

>936_bases
ATGAAAAAACATATTAATGAAAATTTAAAAGATATTTTAATCATTGGTGGTGGACCTGCAGGTTTGACTGCTGGGGTTTA
CGCAGCAAGAGCTGGAATGAAAACAATCATTTTAGAAAAAGAAGCACCAGGTGGGAAAATGGTTAAAACTGACACAATTG
AAAACTACCCAGGCTTTGATAGTATAAAAGGACCAGACCTTGCATTAAAAATGTACATGCAAGTAATGAATCTTGGAGCT
GAATTTGTATATGATGAAGTTATTAAAATTGAAAAAATGGACAATTATTTTATTGTAACTACTTTAAACGGGCAAATAAT
TGAAGCTTTAAGTGTTATTGTTGCAACAGGTACGTTAGAAAATAAATTAGGTATACCAGGTGAAGATAATCTTTATGGAA
AAGGTGTTAGTTATTGTGCTGTTTGTGATGGAGCATTCCATAAAGGAAATCCCGTTGCCATAGTTGGTGGAGGATACTCA
GCTGTAGAAGAGGGCATATACTTAAGTAAATTTGTAAGTAAGTTATATGTTGTTGTAAGAAAAGATCATTTTAGAGTAGA
CCCTGTAACTTTATCTAAATTAGAACAACTTGAAAATGTTGAATTTCTAATGAATTCTGTTGTTAAAAAAGTTAATGGTA
TTGATAAAGTAGAATCTGTTGAAATTGAAAATGTTATTACAAAAGAAATTAAAACTGTACCTGTTACTGGTTTATTTCCA
TATATAGGAGCAACTCCGGTAACTGAATTTTTAGAAAATCTTAATTTAGATAAAAATGAAGGTTACTTAACCGGAGACGC
TAAATTAAAATCTAATATTAAAGGTTTATTCATAGCAGGAGATGTTAGGGATGTTCCTTTAAGACAAATAGCTATTGCTG
CAGGAGATGGAGCATTAGCAGGCCAAATGGCAGTTAATTACGTACAAGAACTATAA

Upstream 100 bases:

>100_bases
AATTTTTTAGTCTTAAGTTTAATAATATTAACAGGAATATTTATTATTTTTATGGCGCAATTTGTAATTCCATATAAATG
AAGAGAAATAGGGTGATTAT

Downstream 100 bases:

>100_bases
AGATATTTAAATGTAGAAAGGTGATCATATGAATATAAAAGATTTTAAGATAAATAAAATTTCGTTTGATTTTAAACGTG
ACAAATTAAAGTGAATTTCA

Product: thioredoxin reductase NADPH

Products: NA

Alternate protein names: TRXR; General stress protein 35; GSP35 [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKKHINENLKDILIIGGGPAGLTAGVYAARAGMKTIILEKEAPGGKMVKTDTIENYPGFDSIKGPDLALKMYMQVMNLGA
EFVYDEVIKIEKMDNYFIVTTLNGQIIEALSVIVATGTLENKLGIPGEDNLYGKGVSYCAVCDGAFHKGNPVAIVGGGYS
AVEEGIYLSKFVSKLYVVVRKDHFRVDPVTLSKLEQLENVEFLMNSVVKKVNGIDKVESVEIENVITKEIKTVPVTGLFP
YIGATPVTEFLENLNLDKNEGYLTGDAKLKSNIKGLFIAGDVRDVPLRQIAIAAGDGALAGQMAVNYVQEL

Sequences:

>Translated_311_residues
MKKHINENLKDILIIGGGPAGLTAGVYAARAGMKTIILEKEAPGGKMVKTDTIENYPGFDSIKGPDLALKMYMQVMNLGA
EFVYDEVIKIEKMDNYFIVTTLNGQIIEALSVIVATGTLENKLGIPGEDNLYGKGVSYCAVCDGAFHKGNPVAIVGGGYS
AVEEGIYLSKFVSKLYVVVRKDHFRVDPVTLSKLEQLENVEFLMNSVVKKVNGIDKVESVEIENVITKEIKTVPVTGLFP
YIGATPVTEFLENLNLDKNEGYLTGDAKLKSNIKGLFIAGDVRDVPLRQIAIAAGDGALAGQMAVNYVQEL
>Mature_311_residues
MKKHINENLKDILIIGGGPAGLTAGVYAARAGMKTIILEKEAPGGKMVKTDTIENYPGFDSIKGPDLALKMYMQVMNLGA
EFVYDEVIKIEKMDNYFIVTTLNGQIIEALSVIVATGTLENKLGIPGEDNLYGKGVSYCAVCDGAFHKGNPVAIVGGGYS
AVEEGIYLSKFVSKLYVVVRKDHFRVDPVTLSKLEQLENVEFLMNSVVKKVNGIDKVESVEIENVITKEIKTVPVTGLFP
YIGATPVTEFLENLNLDKNEGYLTGDAKLKSNIKGLFIAGDVRDVPLRQIAIAAGDGALAGQMAVNYVQEL

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=313, Percent_Identity=33.8658146964856, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI87081763, Length=303, Percent_Identity=34.983498349835, Blast_Score=156, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320560, Length=314, Percent_Identity=36.624203821656, Blast_Score=189, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6321898, Length=311, Percent_Identity=34.7266881028939, Blast_Score=182, Evalue=7e-47,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR005982 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.8.1.9 [H]

Molecular weight: Translated: 33640; Mature: 33640

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHINENLKDILIIGGGPAGLTAGVYAARAGMKTIILEKEAPGGKMVKTDTIENYPGFD
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCCCCCCC
SIKGPDLALKMYMQVMNLGAEFVYDEVIKIEKMDNYFIVTTLNGQIIEALSVIVATGTLE
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHEEECCCEEEEEECCCHHHHHHHHHHHCCCCC
NKLGIPGEDNLYGKGVSYCAVCDGAFHKGNPVAIVGGGYSAVEEGIYLSKFVSKLYVVVR
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHCHHHHHHHHHHEEEEE
KDHFRVDPVTLSKLEQLENVEFLMNSVVKKVNGIDKVESVEIENVITKEIKTVPVTGLFP
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
YIGATPVTEFLENLNLDKNEGYLTGDAKLKSNIKGLFIAGDVRDVPLRQIAIAAGDGALA
HCCCCHHHHHHHHCCCCCCCCEEECCHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCHH
GQMAVNYVQEL
HHHHHHHHHHC
>Mature Secondary Structure
MKKHINENLKDILIIGGGPAGLTAGVYAARAGMKTIILEKEAPGGKMVKTDTIENYPGFD
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCCCCCCC
SIKGPDLALKMYMQVMNLGAEFVYDEVIKIEKMDNYFIVTTLNGQIIEALSVIVATGTLE
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHEEECCCEEEEEECCCHHHHHHHHHHHCCCCC
NKLGIPGEDNLYGKGVSYCAVCDGAFHKGNPVAIVGGGYSAVEEGIYLSKFVSKLYVVVR
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHCHHHHHHHHHHEEEEE
KDHFRVDPVTLSKLEQLENVEFLMNSVVKKVNGIDKVESVEIENVITKEIKTVPVTGLFP
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
YIGATPVTEFLENLNLDKNEGYLTGDAKLKSNIKGLFIAGDVRDVPLRQIAIAAGDGALA
HCCCCHHHHHHHHCCCCCCCCEEECCHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCHH
GQMAVNYVQEL
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 9298659 [H]