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The map label for this gene is ackA [H]

Identifier: 50364859

GI number: 50364859

Start: 59862

End: 61052

Strand: Direct

Name: ackA [H]

Synonym: Mfl044

Alternate gene names: 50364859

Gene position: 59862-61052 (Clockwise)

Preceding gene: 50364858

Following gene: 50364860

Centisome position: 7.55

GC content: 29.05

Gene sequence:

>1191_bases
ATGATTTTAGTAGTTAATGCAGGAAGTAGTTCAATTAAATTTAGATTATTTAATGATTTAGATAAAAGCAATCCAATTGA
TATTCTTGATGGTTTAGCAGAAAGAATAACTGTTGATGGTGCAGTTTCATTTAAATATGAAGGTAAAAAATATGAATATA
ATGTTGAATTACCAAATCATGAAGTAGCAATTAAGTTTATTTTAGATAAATTAATTGAATTAAATATTATCAGCAATGTA
GATGATATAAATGCTGTAGGATTCAGGGTTGTTCATGGTGGAACAATAAGTAAATCTTCAATTATAGATCAAAAAGTCTT
TGACACAATTAAAGATGCAGTTAAATTAGCACCATTGCATAATCCAGGGGCTATAACAGCCATTGAGGCCATTGAAAAAG
TTATGCCTAAAGCTAAATTGGTAGCATGCTTTGATACAGCATATCATCAAACTTTAGCTGAAGAACAATATTTATACGCA
GTTCCTTATTCTTGATATAAAGAACATGGTGTAAGAAAATACGGTTTTCATGGTATTAGTTACCAATACATAGCAGAAAA
AATGTCAGAAGTATTAGCCAAACCTAAAGACCAATTAAATCTTATCGTTTGCCACTTAGGTAACGGTGCAAGTATAACAT
GTATTAAAAATGGTAAATCATTTGATACAACAATGGGATTAACTCCTCTCGCTGGTGTAATGATGGGAACAAGAAGTGGT
GATATTGATCCATCAATTATCGAATACATGTGTAAAGAATTACAATTGGACGTTTCAAAAATAACAAATATTTTAAATAA
AGAATCAGGTCTGTTAGGATTAAGCGGAAAATCAAGTGATATGCGCGATGTAACTGGTGGATATTTTAAAGGTGATGAAG
ATTACAAAAGAGCACTTAACAAATATACACAAGTTTCTGCGGACTATATCATTAGATTTGCAAACTTATTAGGACACAAT
ATTGATGGTATTGTTTTCACAGCTGGTGTTGGTGAAAATTCAATTCATACAAGACAATTTATTTTAGAAAAATTACCTTT
ATTAGATATTGAAATTGATAATGCTAAAAATGAAGAAAGTTATGGAGACTATAAATATATATCTTCACCAAATTCAAAAA
TAAAAGTATTAGCGGTTAGAACTAATGAAGAATTAATGATTTGTAAAGATACAATCAATTTAACTAAATAG

Upstream 100 bases:

>100_bases
GGATTCAATAAACCAGTTAATGATTTAAGTCGTGGAGCTACATTAACAGATATTATGAATACAGCTATTATTACAATTTA
TCAAGCATTGGAGGCGTAAT

Downstream 100 bases:

>100_bases
AATTAAAAATAACCATTAAGGTTATTTTTTTATCTTTTAAATTATAATTAATAATAAATAAATGAGGTGAAACATGAAAA
GAGTATTATTTATAGGTTTA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MILVVNAGSSSIKFRLFNDLDKSNPIDILDGLAERITVDGAVSFKYEGKKYEYNVELPNHEVAIKFILDKLIELNIISNV
DDINAVGFRVVHGGTISKSSIIDQKVFDTIKDAVKLAPLHNPGAITAIEAIEKVMPKAKLVACFDTAYHQTLAEEQYLYA
VPYSWYKEHGVRKYGFHGISYQYIAEKMSEVLAKPKDQLNLIVCHLGNGASITCIKNGKSFDTTMGLTPLAGVMMGTRSG
DIDPSIIEYMCKELQLDVSKITNILNKESGLLGLSGKSSDMRDVTGGYFKGDEDYKRALNKYTQVSADYIIRFANLLGHN
IDGIVFTAGVGENSIHTRQFILEKLPLLDIEIDNAKNEESYGDYKYISSPNSKIKVLAVRTNEELMICKDTINLTK

Sequences:

>Translated_396_residues
MILVVNAGSSSIKFRLFNDLDKSNPIDILDGLAERITVDGAVSFKYEGKKYEYNVELPNHEVAIKFILDKLIELNIISNV
DDINAVGFRVVHGGTISKSSIIDQKVFDTIKDAVKLAPLHNPGAITAIEAIEKVMPKAKLVACFDTAYHQTLAEEQYLYA
VPYS*YKEHGVRKYGFHGISYQYIAEKMSEVLAKPKDQLNLIVCHLGNGASITCIKNGKSFDTTMGLTPLAGVMMGTRSG
DIDPSIIEYMCKELQLDVSKITNILNKESGLLGLSGKSSDMRDVTGGYFKGDEDYKRALNKYTQVSADYIIRFANLLGHN
IDGIVFTAGVGENSIHTRQFILEKLPLLDIEIDNAKNEESYGDYKYISSPNSKIKVLAVRTNEELMICKDTINLTK
>Mature_396_residues
MILVVNAGSSSIKFRLFNDLDKSNPIDILDGLAERITVDGAVSFKYEGKKYEYNVELPNHEVAIKFILDKLIELNIISNV
DDINAVGFRVVHGGTISKSSIIDQKVFDTIKDAVKLAPLHNPGAITAIEAIEKVMPKAKLVACFDTAYHQTLAEEQYLYA
VPYS*YKEHGVRKYGFHGISYQYIAEKMSEVLAKPKDQLNLIVCHLGNGASITCIKNGKSFDTTMGLTPLAGVMMGTRSG
DIDPSIIEYMCKELQLDVSKITNILNKESGLLGLSGKSSDMRDVTGGYFKGDEDYKRALNKYTQVSADYIIRFANLLGHN
IDGIVFTAGVGENSIHTRQFILEKLPLLDIEIDNAKNEESYGDYKYISSPNSKIKVLAVRTNEELMICKDTINLTK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=405, Percent_Identity=43.7037037037037, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI145693184, Length=400, Percent_Identity=39.75, Blast_Score=286, Evalue=1e-78,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43798; Mature: 43798

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVVNAGSSSIKFRLFNDLDKSNPIDILDGLAERITVDGAVSFKYEGKKYEYNVELPNH
CEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHEEECCEEEEEECCCEEEEEEECCCC
EVAIKFILDKLIELNIISNVDDINAVGFRVVHGGTISKSSIIDQKVFDTIKDAVKLAPLH
HHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
NPGAITAIEAIEKVMPKAKLVACFDTAYHQTLAEEQYLYAVPYSYKEHGVRKYGFHGISY
CCCCHHHHHHHHHHCCCHHEEEHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHCCCCCHH
QYIAEKMSEVLAKPKDQLNLIVCHLGNGASITCIKNGKSFDTTMGLTPLAGVMMGTRSGD
HHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCCC
IDPSIIEYMCKELQLDVSKITNILNKESGLLGLSGKSSDMRDVTGGYFKGDEDYKRALNK
CCHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCHHCCCCCCCCCHHHHHHHHH
YTQVSADYIIRFANLLGHNIDGIVFTAGVGENSIHTRQFILEKLPLLDIEIDNAKNEESY
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCC
GDYKYISSPNSKIKVLAVRTNEELMICKDTINLTK
CCEEEECCCCCEEEEEEEECCCCEEEEECCCCCCC
>Mature Secondary Structure
MILVVNAGSSSIKFRLFNDLDKSNPIDILDGLAERITVDGAVSFKYEGKKYEYNVELPNH
CEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHEEECCEEEEEECCCEEEEEEECCCC
EVAIKFILDKLIELNIISNVDDINAVGFRVVHGGTISKSSIIDQKVFDTIKDAVKLAPLH
HHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
NPGAITAIEAIEKVMPKAKLVACFDTAYHQTLAEEQYLYAVPYSYKEHGVRKYGFHGISY
CCCCHHHHHHHHHHCCCHHEEEHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHCCCCCHH
QYIAEKMSEVLAKPKDQLNLIVCHLGNGASITCIKNGKSFDTTMGLTPLAGVMMGTRSGD
HHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCCC
IDPSIIEYMCKELQLDVSKITNILNKESGLLGLSGKSSDMRDVTGGYFKGDEDYKRALNK
CCHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCHHCCCCCCCCCHHHHHHHHH
YTQVSADYIIRFANLLGHNIDGIVFTAGVGENSIHTRQFILEKLPLLDIEIDNAKNEESY
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCC
GDYKYISSPNSKIKVLAVRTNEELMICKDTINLTK
CCEEEECCCCCEEEEEEEECCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA