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The map label for this gene is lplA [H]

Identifier: 50364853

GI number: 50364853

Start: 52658

End: 53662

Strand: Direct

Name: lplA [H]

Synonym: Mfl038

Alternate gene names: 50364853

Gene position: 52658-53662 (Clockwise)

Preceding gene: 50364852

Following gene: 50364854

Centisome position: 6.64

GC content: 25.27

Gene sequence:

>1005_bases
ATGATTAATCTATTTATTTCAAAATCAAACGATCCAGCATACAACTTAGCTGTTGAAGAGTATTTGACATATCATTACCA
AACTAAAGACCCTATTTTGTATATTTGACAAAATAGCAACACAATTGTTGTTGGTAGAAATCAAAATACTTATGCTGAAA
TTAACATTGCTGAAGCGATGAAAGATGAAGTAAAAATTATAAGAAGAAACACGGGTGGAGGTACTGTTTTCCATGATATG
GGAAATGTGTGTTATTCACTAATTGTTAACAATGATAAAAATTCACAATCTAACTTTGAAATTGCTTTACAACCAATTAT
TCAGTATTTAAGATCTGAAGGTTTAAATGCTAATTTCTCAGGAAGAAATGATATTGAAATTGACGGTTATAAAATATCAG
GAAATGCACAATTAAAAACAAATAATAAAATTCTACAACATGGTACATTATTGTTTGATGTTGAATTACCAAGAATTTTA
AAATATTTAAATGTAGATCCTGAAAAAATTAAACACCAAAAAGTTAAATCTAAACCAGCTAGAGTTGCAAATATAAAAGC
ACTTTTAGAAGGTATAAATAAAAATATTGAACTTACTGACTTTATAAACAATATAATAAATAGCTATACAAAAAATGAAG
AAACTAAATGAATCGAATTTAGTGAAACTGATGAATTAGCTATTAATAAATGTTTTGAAGAAAAGTATCAATCTCGTGAT
TGAACATTTGCTAAAAATGAAAATTTTGAAATCAGTAACAAGAAATACTTAGAATCAAAAGGTTTAACAGAAATTAAAAT
AAATGTTGATAAGGGAAAAATCCAAAATATTAAAATTTATGGGGACTTCTTAGGGTACATTGGAACTGAAGCATTAGAAT
TTTCATTATTAAATGTTAATTATGATTATCACTCAGTTAAAAACATTCTAGATAAGTTTGCATTAAAAGAAATATTTGGT
GATAACTTTGAAGCAGAAGATATTTTGAATTTAATATTCAATTAA

Upstream 100 bases:

>100_bases
TTAGTTGATATATTCTTCTTACCTCACTTCAACAAGCCATACAACTTTGTTACATTAGCAGGTTTAGAAGTATTAGGATT
AAATTACTTTAAAAAATAAG

Downstream 100 bases:

>100_bases
GAAAGGTATAAATATGAAATATATAGGAAAATTTGATCCTCAAAAAGATGAAATTGTTCGTGTAATGGATAAAGATGGTA
AAATCATTAATCCTAAATTA

Product: lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MINLFISKSNDPAYNLAVEEYLTYHYQTKDPILYIWQNSNTIVVGRNQNTYAEINIAEAMKDEVKIIRRNTGGGTVFHDM
GNVCYSLIVNNDKNSQSNFEIALQPIIQYLRSEGLNANFSGRNDIEIDGYKISGNAQLKTNNKILQHGTLLFDVELPRIL
KYLNVDPEKIKHQKVKSKPARVANIKALLEGINKNIELTDFINNIINSYTKNEETKWIEFSETDELAINKCFEEKYQSRD
WTFAKNENFEISNKKYLESKGLTEIKINVDKGKIQNIKIYGDFLGYIGTEALEFSLLNVNYDYHSVKNILDKFALKEIFG
DNFEAEDILNLIFN

Sequences:

>Translated_334_residues
MINLFISKSNDPAYNLAVEEYLTYHYQTKDPILYI*QNSNTIVVGRNQNTYAEINIAEAMKDEVKIIRRNTGGGTVFHDM
GNVCYSLIVNNDKNSQSNFEIALQPIIQYLRSEGLNANFSGRNDIEIDGYKISGNAQLKTNNKILQHGTLLFDVELPRIL
KYLNVDPEKIKHQKVKSKPARVANIKALLEGINKNIELTDFINNIINSYTKNEETK*IEFSETDELAINKCFEEKYQSRD
*TFAKNENFEISNKKYLESKGLTEIKINVDKGKIQNIKIYGDFLGYIGTEALEFSLLNVNYDYHSVKNILDKFALKEIFG
DNFEAEDILNLIFN
>Mature_334_residues
MINLFISKSNDPAYNLAVEEYLTYHYQTKDPILYI*QNSNTIVVGRNQNTYAEINIAEAMKDEVKIIRRNTGGGTVFHDM
GNVCYSLIVNNDKNSQSNFEIALQPIIQYLRSEGLNANFSGRNDIEIDGYKISGNAQLKTNNKILQHGTLLFDVELPRIL
KYLNVDPEKIKHQKVKSKPARVANIKALLEGINKNIELTDFINNIINSYTKNEETK*IEFSETDELAINKCFEEKYQSRD
*TFAKNENFEISNKKYLESKGLTEIKINVDKGKIQNIKIYGDFLGYIGTEALEFSLLNVNYDYHSVKNILDKFALKEIFG
DNFEAEDILNLIFN

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=279, Percent_Identity=28.673835125448, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI21729884, Length=279, Percent_Identity=28.673835125448, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI21729882, Length=279, Percent_Identity=28.673835125448, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI21729880, Length=279, Percent_Identity=28.673835125448, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI21729878, Length=279, Percent_Identity=28.673835125448, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1790846, Length=310, Percent_Identity=30, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71981909, Length=267, Percent_Identity=28.8389513108614, Blast_Score=85, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322415, Length=192, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI28573464, Length=232, Percent_Identity=24.5689655172414, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37918; Mature: 37918

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLFISKSNDPAYNLAVEEYLTYHYQTKDPILYIQNSNTIVVGRNQNTYAEINIAEAMK
CEEEEEECCCCCCEEEEHHHHHEEEEECCCCEEEEECCCEEEEECCCCCEEEEEHHHHHH
DEVKIIRRNTGGGTVFHDMGNVCYSLIVNNDKNSQSNFEIALQPIIQYLRSEGLNANFSG
HHHHEEEECCCCCEEHHHHHHHEEEEEECCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCC
RNDIEIDGYKISGNAQLKTNNKILQHGTLLFDVELPRILKYLNVDPEKIKHQKVKSKPAR
CCCEEEECEEECCCEEEEECCEEEECCCEEEEECHHHHHHHHCCCHHHHHHHHHHCCCCH
VANIKALLEGINKNIELTDFINNIINSYTKNEETKIEFSETDELAINKCFEEKYQSRDTF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCC
AKNENFEISNKKYLESKGLTEIKINVDKGKIQNIKIYGDFLGYIGTEALEFSLLNVNYDY
CCCCCEEECCCHHHHCCCCEEEEEEECCCCEEEEEEEEHHHHHHCCHHEEEEEEEECCCH
HSVKNILDKFALKEIFGDNFEAEDILNLIFN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MINLFISKSNDPAYNLAVEEYLTYHYQTKDPILYIQNSNTIVVGRNQNTYAEINIAEAMK
CEEEEEECCCCCCEEEEHHHHHEEEEECCCCEEEEECCCEEEEECCCCCEEEEEHHHHHH
DEVKIIRRNTGGGTVFHDMGNVCYSLIVNNDKNSQSNFEIALQPIIQYLRSEGLNANFSG
HHHHEEEECCCCCEEHHHHHHHEEEEEECCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCC
RNDIEIDGYKISGNAQLKTNNKILQHGTLLFDVELPRILKYLNVDPEKIKHQKVKSKPAR
CCCEEEECEEECCCEEEEECCEEEECCCEEEEECHHHHHHHHCCCHHHHHHHHHHCCCCH
VANIKALLEGINKNIELTDFINNIINSYTKNEETKIEFSETDELAINKCFEEKYQSRDTF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCC
AKNENFEISNKKYLESKGLTEIKINVDKGKIQNIKIYGDFLGYIGTEALEFSLLNVNYDY
CCCCCEEECCCHHHHCCCCEEEEEEECCCCEEEEEEEEHHHHHHCCHHEEEEEEEECCCH
HSVKNILDKFALKEIFGDNFEAEDILNLIFN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]