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The map label for this gene is bglH [H]

Identifier: 50364824

GI number: 50364824

Start: 12376

End: 13839

Strand: Direct

Name: bglH [H]

Synonym: Mfl009

Alternate gene names: 50364824

Gene position: 12376-13839 (Clockwise)

Preceding gene: 50364823

Following gene: 50364825

Centisome position: 1.56

GC content: 28.48

Gene sequence:

>1464_bases
ATGAAAAAATTAAATTTCCCAAAATCATTTTTATGAGGTGGAGCAACATCTGCTGCTCAAATAGAGGGTGCATGAAATAT
AGATGGTAAGTCACTCACTTTGCCAGAAATACAGCCCTTTATAGAGCTAAAAGATAAAAGTGATTTATCAAAATTACATA
ATGAAAGAAATATAATTTTTAAAAATGCTTTAGAAGGTAAGTTCGAAGGTCATTATCCAAAAAGATTTGGAATAGATTTT
TATCATAGATATAAGGAAGATATTGCTTTATTCAAAGAAGCTGGAATGAACATATTTAGAATGTCTATTTCTTGGGCAAG
AATATTCCCAAATGCATTCGATGAAAAACCAAATTTAAATGGCCTTAAATTTTACAGAGATGTGTTTGAAGAATGTAAAA
AAAATAATATGGAAATCATGGTTACAATGAGTCACTTTGATTATCCTTTTGAATTAATGAAGTCAAACCCAAAAGGATGG
CTAGATCCAAAAGTTAAAGAATTATTTTTAAAGTATGCAAAAACAATATTAGATGAGTATGCAGATATAGTTAAATATTG
ACTTCCTTTTAATGAAATTAATATAGGTGCTTTTGCTGTAGAGGCAGGCTTAGGTATTATGCCAGATAGCAAAAGAACTG
AAGCAGAAACTAAAGAGCTTTCAATAAAAGGTCTTCATAATCTATTTGTTGCGCAAGCAGAAATAGTAAAGTATGCAAAA
AAATTTAAAAATATTAAATTAGGTTGTATGATGGCAGATATGACAACTTATTCACTAGATTGCAACCCTGTTAATGTTCT
TGCAAATATGAAGAGAGAACAAATACTTAAGTTCATGTTTTACGATGTAATGATCAAAGGAAAATATCCAGGTTACTTTA
AAAATATCGTAAAAACATTGACTAATAAAGAAATAACTTTTGATGAAAATGAGTTAAAACTTTTAAAAGAAAATCCTCTT
GAATTTATAAGCTTCAGTTATTATATGTCTAACACAGTTTCAACTGATCCAAATCAAGAAAAAACTCAAGGTAATTTAAC
ATCAGCTGGAAAAAATCCGTTTTTGAAAGCAACAGATTGAGGATGACAAATTGATCCAATTGGATTAAGATATGTTTTAA
ATCAGCTATGAGATAGATATGAAGTTCCTTTATTTATTTCAGAAAATGGAATTGGTGTTTTAGAAACACTAAATGAAGAA
AATGCAGTTAATGATGACTATCGTATAGACTATCTAAGTAAACATATTGAACAAATAGATTTAGCCCTAAAAGACGGTGT
TGATGTTTTTGGATATACAATGTGAACTCCAATAGATCTTGTATCTGCAAGCACCTGTGAAATGGCAAAAAGATATGGAT
TAATATTTGTAGATTATGATGATTATCATAACGGGACTGGTAACAGATATCCCAAAAAATCATTTAATTGATTTAAAGAA
TTTATTAAAACAAAAGAAATTTAA

Upstream 100 bases:

>100_bases
GAAAAAGAAAATAATGTTGATTTATCAAAATATAAAAATGAATACTCAAAAGATGTTGATTCATTGTTATTAAATTATGC
TTTATAGAGAGAATTAAAAA

Downstream 100 bases:

>100_bases
AATAGTGCTCTTTAAGGGCACTATTTTATTTTATAATTATAAAAGAATAGAGGTTTTTATGAAAGAAAAAATGGCTTTTA
GATTTCTTGAAGAAATAGCT

Product: beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKKLNFPKSFLWGGATSAAQIEGAWNIDGKSLTLPEIQPFIELKDKSDLSKLHNERNIIFKNALEGKFEGHYPKRFGIDF
YHRYKEDIALFKEAGMNIFRMSISWARIFPNAFDEKPNLNGLKFYRDVFEECKKNNMEIMVTMSHFDYPFELMKSNPKGW
LDPKVKELFLKYAKTILDEYADIVKYWLPFNEINIGAFAVEAGLGIMPDSKRTEAETKELSIKGLHNLFVAQAEIVKYAK
KFKNIKLGCMMADMTTYSLDCNPVNVLANMKREQILKFMFYDVMIKGKYPGYFKNIVKTLTNKEITFDENELKLLKENPL
EFISFSYYMSNTVSTDPNQEKTQGNLTSAGKNPFLKATDWGWQIDPIGLRYVLNQLWDRYEVPLFISENGIGVLETLNEE
NAVNDDYRIDYLSKHIEQIDLALKDGVDVFGYTMWTPIDLVSASTCEMAKRYGLIFVDYDDYHNGTGNRYPKKSFNWFKE
FIKTKEI

Sequences:

>Translated_487_residues
MKKLNFPKSFL*GGATSAAQIEGA*NIDGKSLTLPEIQPFIELKDKSDLSKLHNERNIIFKNALEGKFEGHYPKRFGIDF
YHRYKEDIALFKEAGMNIFRMSISWARIFPNAFDEKPNLNGLKFYRDVFEECKKNNMEIMVTMSHFDYPFELMKSNPKGW
LDPKVKELFLKYAKTILDEYADIVKY*LPFNEINIGAFAVEAGLGIMPDSKRTEAETKELSIKGLHNLFVAQAEIVKYAK
KFKNIKLGCMMADMTTYSLDCNPVNVLANMKREQILKFMFYDVMIKGKYPGYFKNIVKTLTNKEITFDENELKLLKENPL
EFISFSYYMSNTVSTDPNQEKTQGNLTSAGKNPFLKATD*G*QIDPIGLRYVLNQL*DRYEVPLFISENGIGVLETLNEE
NAVNDDYRIDYLSKHIEQIDLALKDGVDVFGYTM*TPIDLVSASTCEMAKRYGLIFVDYDDYHNGTGNRYPKKSFN*FKE
FIKTKEI
>Mature_487_residues
MKKLNFPKSFL*GGATSAAQIEGA*NIDGKSLTLPEIQPFIELKDKSDLSKLHNERNIIFKNALEGKFEGHYPKRFGIDF
YHRYKEDIALFKEAGMNIFRMSISWARIFPNAFDEKPNLNGLKFYRDVFEECKKNNMEIMVTMSHFDYPFELMKSNPKGW
LDPKVKELFLKYAKTILDEYADIVKY*LPFNEINIGAFAVEAGLGIMPDSKRTEAETKELSIKGLHNLFVAQAEIVKYAK
KFKNIKLGCMMADMTTYSLDCNPVNVLANMKREQILKFMFYDVMIKGKYPGYFKNIVKTLTNKEITFDENELKLLKENPL
EFISFSYYMSNTVSTDPNQEKTQGNLTSAGKNPFLKATD*G*QIDPIGLRYVLNQL*DRYEVPLFISENGIGVLETLNEE
NAVNDDYRIDYLSKHIEQIDLALKDGVDVFGYTM*TPIDLVSASTCEMAKRYGLIFVDYDDYHNGTGNRYPKKSFN*FKE
FIKTKEI

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=440, Percent_Identity=25, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI110681710, Length=500, Percent_Identity=24.2, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI13273313, Length=475, Percent_Identity=24.2105263157895, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI28376633, Length=483, Percent_Identity=23.6024844720497, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI24497614, Length=387, Percent_Identity=25.8397932816537, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI2367270, Length=483, Percent_Identity=40.7867494824017, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1789070, Length=478, Percent_Identity=39.9581589958159, Blast_Score=315, Evalue=6e-87,
Organism=Escherichia coli, GI2367174, Length=490, Percent_Identity=39.1836734693878, Blast_Score=303, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17539390, Length=505, Percent_Identity=29.1089108910891, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17552856, Length=496, Percent_Identity=28.8306451612903, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21356577, Length=510, Percent_Identity=23.5294117647059, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55017; Mature: 55017

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLNFPKSFLGGATSAAQIEGANIDGKSLTLPEIQPFIELKDKSDLSKLHNERNIIFKN
CCCCCCCHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHEECCCHHHHHHHCCCCEEEEC
ALEGKFEGHYPKRFGIDFYHRYKEDIALFKEAGMNIFRMSISWARIFPNAFDEKPNLNGL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCHHCCCCCCCHH
KFYRDVFEECKKNNMEIMVTMSHFDYPFELMKSNPKGWLDPKVKELFLKYAKTILDEYAD
HHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IVKYLPFNEINIGAFAVEAGLGIMPDSKRTEAETKELSIKGLHNLFVAQAEIVKYAKKFK
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NIKLGCMMADMTTYSLDCNPVNVLANMKREQILKFMFYDVMIKGKYPGYFKNIVKTLTNK
CCEEEEEEECCEEEEECCCHHHHHHHCCHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCC
EITFDENELKLLKENPLEFISFSYYMSNTVSTDPNQEKTQGNLTSAGKNPFLKATDGQID
CEEECCHHHHHHHCCCHHHEEEHHHHCCCCCCCCCCHHHCCCCCCCCCCCCEECCCCCCC
PIGLRYVLNQLDRYEVPLFISENGIGVLETLNEENAVNDDYRIDYLSKHIEQIDLALKDG
CHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
VDVFGYTMTPIDLVSASTCEMAKRYGLIFVDYDDYHNGTGNRYPKKSFNFKEFIKTKEI
CCEEEEEECHHHHHCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MKKLNFPKSFLGGATSAAQIEGANIDGKSLTLPEIQPFIELKDKSDLSKLHNERNIIFKN
CCCCCCCHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHEECCCHHHHHHHCCCCEEEEC
ALEGKFEGHYPKRFGIDFYHRYKEDIALFKEAGMNIFRMSISWARIFPNAFDEKPNLNGL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCHHCCCCCCCHH
KFYRDVFEECKKNNMEIMVTMSHFDYPFELMKSNPKGWLDPKVKELFLKYAKTILDEYAD
HHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IVKYLPFNEINIGAFAVEAGLGIMPDSKRTEAETKELSIKGLHNLFVAQAEIVKYAKKFK
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NIKLGCMMADMTTYSLDCNPVNVLANMKREQILKFMFYDVMIKGKYPGYFKNIVKTLTNK
CCEEEEEEECCEEEEECCCHHHHHHHCCHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCC
EITFDENELKLLKENPLEFISFSYYMSNTVSTDPNQEKTQGNLTSAGKNPFLKATDGQID
CEEECCHHHHHHHCCCHHHEEEHHHHCCCCCCCCCCHHHCCCCCCCCCCCCEECCCCCCC
PIGLRYVLNQLDRYEVPLFISENGIGVLETLNEENAVNDDYRIDYLSKHIEQIDLALKDG
CHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
VDVFGYTMTPIDLVSASTCEMAKRYGLIFVDYDDYHNGTGNRYPKKSFNFKEFIKTKEI
CCEEEEEECHHHHHCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7883710; 7704263; 9384377 [H]