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The map label for this gene is gyrA [H]

Identifier: 50364822

GI number: 50364822

Start: 7236

End: 9713

Strand: Direct

Name: gyrA [H]

Synonym: Mfl007

Alternate gene names: 50364822

Gene position: 7236-9713 (Clockwise)

Preceding gene: 50364821

Following gene: 50364823

Centisome position: 0.91

GC content: 31.44

Gene sequence:

>2478_bases
ATGAGTGAAAAAAACTTAGAACATAACCACGGTATTATCAAGGGTATTGATATTGCTGCTGAGGTTAGAAAAGACTTTTT
AGAATATTCAATGTCTGTTATTGTTAGTCGTGCGCTTCCAGACTTGAAAGATGGTTTAAAACCTGTTCACCGTCGTATTA
TTTATGCGATGAATGACTTAGGTATTACTGCAGATAAACCACACAAAAAATCAGCACGTATTGTTGGGGAAGTTATTGGT
AAGTACCACCCCCATGGTGATACAGCTGTTTATGATTCAATGGTAAGAATGGCTCAAGACTTTTCATACCGTTACCCATT
AGTTGATGGACATGGTAACTTTGGTTCAATCGATGGTGATGGAGCTGCTGCTATGCGTTATACCGAAGCTAGATTAGCTA
AAGTATCAAACTTCATCATTAAAGATATTGATATGAACACCGTTCCTTTTGTTGATAACTATGATGCTAGTGAAAGAGAA
CCTGCTTATTTAACAGGATACTTCCCTAACTTATTAGCCAATGGAGCTATGGGTATTGCTGTTGGGATGGCAACAAGTAT
TCCACCCCACAACTTAAGAGAAGTAATTAGTGCAATTCATGCTTTCATTGATAATAAAGACATTACAATTGATGAAATTT
TAGATAACCACATTATGGGACCAGATTTCCCAACAGGTGCGTTAATGACAAATGGAATCAGAATGCGTGAAGGTTATAAA
ACTGGTAGAGGTGCTGTAACTATTCGTGCTAAAGTAGCTCTTGAAGAAAATGATCGTCATGCAAGATTTATAATTACTGA
AATTCCATACCAAACTAATAAAGCTAAATTAATTGAAAGAATTGCTGAATTGGTTAAAACAAAACAATTAGAAGGTATCT
CAGATATTCGTGATGAATCTAACTATGAAGGTATCAGAATTGTTATTGAATTAAGACGTGATTCAAATCCAGATGTTGTT
TTATCTAAGTTATATAAATTTACTAACTTACAAACAACATATTCATTAAACTTATTATCATTACATAATAACATTCCTGT
GTTATTGGATTTAAAATCAATTATTAAACATTATGTTGATTTCCAAATCAATGTAATTATTAAACGTTCAATTTTTGAAA
AAGATAAAATTGAAAAACGTTTCCACATCTTAGAGGCTTTAGATACTGCATTAGATAACATTGATGCTATTGTTAATATT
TTAAGAAACTCACCAGAGTCAAACGAAGCTAAAATGAAATTAACAGAAGCGTTTGGATTTGATGAAGAACAAAACAAAGC
TATCTTAGATATGAGATTACAACGTTTAGTTGGATTAGAACGTGGAAAAATTCAACAAGAAATGGCTCAAATCAAAGAAA
GAATTGATTATTTAACATTATTAATTTCTGATGAAACAGAACAAAACAATGTTCTTAAAAATCAATTATCTGAAATTGCT
GAAAAATTCGGTGATAACAGAAGAACTGAAACAATTGATGAAGGATTAATGGACATCGAAGATGAAGAATTAATTCCTGA
TGTTAAAACAATGATTCTTTTAAGTGATGAAGGATATATCCGTCGTGTTGATCCAGAAGAATTTAGAGTTCAAAAACGTG
GTGGTAGAGGAGTTAGTGTTAACTCATCTAATGAAGACCCAATTGTAATTGCTACAATGGGTAAAATGCGTGACTGAGTA
TTATTCTTTACTAATTCAGGAAAAGTATTTAGAACAAAAGCATATAACATTAGACAATACTCACGTACAGCACGTGGATT
ACCAATTGTTAACTTCTTAAATGGATTAACAGCTGGAGATAAAATTACAGCTATTCTTCCATTAAGAAATGTAAAAGAAA
AAATGAATTACTTAACTTTCATTACTGAAAAAGGAATGATTAAAAAAACAGATATTTCATTATTCGACAATATTAACAAG
AATGGTAAGATTGCTATTAACTTAAAAGAAGATGATCAATTAGTAACAGTATTTGCAACAACTGGAGAAGATACAATTTT
TGTTGCTAATAAATCAGGAAAAGTTATTAGAATCCAAGAAAATATTGTTAGACCACTTTCAAGAACAGCATCAGGAGTTA
AAGCTATTAAACTTGATGAAAATGATGTTGTGGTTGGAGCAGTTACTTCATTTGGAATTGAAAACATAACAACTATTTCA
TCAAAAGGAAGCTTCAAGAAAACAAATATTGATGAATATAGAATTTCAGGCCGTAATGGAAAAGGAATTAAAGTAATGAA
CCTAAATGAAAAAACAGGTGACTTTAAATCAATTATTGCAGCTAGAGAAACTGATTTAGTATGTATTATTTCAACTGATG
GAAACTTAATTAAAACTAAAGCATCAGACATTCCTGTTTTAGGAAGAGCTGCGGCGGGAGTTAGAGGTATTCGTTTAAGT
GAAGGTAATAAAATTCAAGCAGTAATGCTTGAGTACCGTAAACATGGAGAAGAAAACCAAGAATTCGAAGAAGATTAA

Upstream 100 bases:

>100_bases
TGGGAGAAGACCCAGAACTAAGAAGAAATTATATTCAAGAAAACGCAGAGTTTGTTGAAAACATTGACTTCTAGACAAAG
AATAGAAAAGGTGAATTAAA

Downstream 100 bases:

>100_bases
AAAAGAGGGCAACCTCTTTTTTTTATAAAGTATTTCGATAAAAACGAAATACTTTATAATTTTAAGATGCTAAAAGAAAA
AGCTTTATATTATGGGAAAA

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 825; Mature: 824

Protein sequence:

>825_residues
MSEKNLEHNHGIIKGIDIAAEVRKDFLEYSMSVIVSRALPDLKDGLKPVHRRIIYAMNDLGITADKPHKKSARIVGEVIG
KYHPHGDTAVYDSMVRMAQDFSYRYPLVDGHGNFGSIDGDGAAAMRYTEARLAKVSNFIIKDIDMNTVPFVDNYDASERE
PAYLTGYFPNLLANGAMGIAVGMATSIPPHNLREVISAIHAFIDNKDITIDEILDNHIMGPDFPTGALMTNGIRMREGYK
TGRGAVTIRAKVALEENDRHARFIITEIPYQTNKAKLIERIAELVKTKQLEGISDIRDESNYEGIRIVIELRRDSNPDVV
LSKLYKFTNLQTTYSLNLLSLHNNIPVLLDLKSIIKHYVDFQINVIIKRSIFEKDKIEKRFHILEALDTALDNIDAIVNI
LRNSPESNEAKMKLTEAFGFDEEQNKAILDMRLQRLVGLERGKIQQEMAQIKERIDYLTLLISDETEQNNVLKNQLSEIA
EKFGDNRRTETIDEGLMDIEDEELIPDVKTMILLSDEGYIRRVDPEEFRVQKRGGRGVSVNSSNEDPIVIATMGKMRDWV
LFFTNSGKVFRTKAYNIRQYSRTARGLPIVNFLNGLTAGDKITAILPLRNVKEKMNYLTFITEKGMIKKTDISLFDNINK
NGKIAINLKEDDQLVTVFATTGEDTIFVANKSGKVIRIQENIVRPLSRTASGVKAIKLDENDVVVGAVTSFGIENITTIS
SKGSFKKTNIDEYRISGRNGKGIKVMNLNEKTGDFKSIIAARETDLVCIISTDGNLIKTKASDIPVLGRAAAGVRGIRLS
EGNKIQAVMLEYRKHGEENQEFEED

Sequences:

>Translated_825_residues
MSEKNLEHNHGIIKGIDIAAEVRKDFLEYSMSVIVSRALPDLKDGLKPVHRRIIYAMNDLGITADKPHKKSARIVGEVIG
KYHPHGDTAVYDSMVRMAQDFSYRYPLVDGHGNFGSIDGDGAAAMRYTEARLAKVSNFIIKDIDMNTVPFVDNYDASERE
PAYLTGYFPNLLANGAMGIAVGMATSIPPHNLREVISAIHAFIDNKDITIDEILDNHIMGPDFPTGALMTNGIRMREGYK
TGRGAVTIRAKVALEENDRHARFIITEIPYQTNKAKLIERIAELVKTKQLEGISDIRDESNYEGIRIVIELRRDSNPDVV
LSKLYKFTNLQTTYSLNLLSLHNNIPVLLDLKSIIKHYVDFQINVIIKRSIFEKDKIEKRFHILEALDTALDNIDAIVNI
LRNSPESNEAKMKLTEAFGFDEEQNKAILDMRLQRLVGLERGKIQQEMAQIKERIDYLTLLISDETEQNNVLKNQLSEIA
EKFGDNRRTETIDEGLMDIEDEELIPDVKTMILLSDEGYIRRVDPEEFRVQKRGGRGVSVNSSNEDPIVIATMGKMRD*V
LFFTNSGKVFRTKAYNIRQYSRTARGLPIVNFLNGLTAGDKITAILPLRNVKEKMNYLTFITEKGMIKKTDISLFDNINK
NGKIAINLKEDDQLVTVFATTGEDTIFVANKSGKVIRIQENIVRPLSRTASGVKAIKLDENDVVVGAVTSFGIENITTIS
SKGSFKKTNIDEYRISGRNGKGIKVMNLNEKTGDFKSIIAARETDLVCIISTDGNLIKTKASDIPVLGRAAAGVRGIRLS
EGNKIQAVMLEYRKHGEENQEFEED
>Mature_824_residues
SEKNLEHNHGIIKGIDIAAEVRKDFLEYSMSVIVSRALPDLKDGLKPVHRRIIYAMNDLGITADKPHKKSARIVGEVIGK
YHPHGDTAVYDSMVRMAQDFSYRYPLVDGHGNFGSIDGDGAAAMRYTEARLAKVSNFIIKDIDMNTVPFVDNYDASEREP
AYLTGYFPNLLANGAMGIAVGMATSIPPHNLREVISAIHAFIDNKDITIDEILDNHIMGPDFPTGALMTNGIRMREGYKT
GRGAVTIRAKVALEENDRHARFIITEIPYQTNKAKLIERIAELVKTKQLEGISDIRDESNYEGIRIVIELRRDSNPDVVL
SKLYKFTNLQTTYSLNLLSLHNNIPVLLDLKSIIKHYVDFQINVIIKRSIFEKDKIEKRFHILEALDTALDNIDAIVNIL
RNSPESNEAKMKLTEAFGFDEEQNKAILDMRLQRLVGLERGKIQQEMAQIKERIDYLTLLISDETEQNNVLKNQLSEIAE
KFGDNRRTETIDEGLMDIEDEELIPDVKTMILLSDEGYIRRVDPEEFRVQKRGGRGVSVNSSNEDPIVIATMGKMRD*VL
FFTNSGKVFRTKAYNIRQYSRTARGLPIVNFLNGLTAGDKITAILPLRNVKEKMNYLTFITEKGMIKKTDISLFDNINKN
GKIAINLKEDDQLVTVFATTGEDTIFVANKSGKVIRIQENIVRPLSRTASGVKAIKLDENDVVVGAVTSFGIENITTISS
KGSFKKTNIDEYRISGRNGKGIKVMNLNEKTGDFKSIIAARETDLVCIISTDGNLIKTKASDIPVLGRAAAGVRGIRLSE
GNKIQAVMLEYRKHGEENQEFEED

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=343, Percent_Identity=26.2390670553936, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI19913406, Length=345, Percent_Identity=24.0579710144928, Blast_Score=87, Evalue=6e-17,
Organism=Escherichia coli, GI1788562, Length=826, Percent_Identity=42.8571428571429, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=662, Percent_Identity=35.9516616314199, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI212645657, Length=215, Percent_Identity=31.1627906976744, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535065, Length=174, Percent_Identity=33.9080459770115, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI212645845, Length=500, Percent_Identity=22.2, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI115534553, Length=170, Percent_Identity=30, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=200, Percent_Identity=28.5, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136538, Length=355, Percent_Identity=24.7887323943662, Blast_Score=76, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 92547; Mature: 92416

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKNLEHNHGIIKGIDIAAEVRKDFLEYSMSVIVSRALPDLKDGLKPVHRRIIYAMNDL
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GITADKPHKKSARIVGEVIGKYHPHGDTAVYDSMVRMAQDFSYRYPLVDGHGNFGSIDGD
CCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
GAAAMRYTEARLAKVSNFIIKDIDMNTVPFVDNYDASEREPAYLTGYFPNLLANGAMGIA
CCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHCCCCCEEE
VGMATSIPPHNLREVISAIHAFIDNKDITIDEILDNHIMGPDFPTGALMTNGIRMREGYK
EEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCHHHCCCEEEECCCC
TGRGAVTIRAKVALEENDRHARFIITEIPYQTNKAKLIERIAELVKTKQLEGISDIRDES
CCCCEEEEEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCC
NYEGIRIVIELRRDSNPDVVLSKLYKFTNLQTTYSLNLLSLHNNIPVLLDLKSIIKHYVD
CCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCEEEEHHHHHHHHHC
FQINVIIKRSIFEKDKIEKRFHILEALDTALDNIDAIVNILRNSPESNEAKMKLTEAFGF
EEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHCCC
DEEQNKAILDMRLQRLVGLERGKIQQEMAQIKERIDYLTLLISDETEQNNVLKNQLSEIA
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHH
EKFGDNRRTETIDEGLMDIEDEELIPDVKTMILLSDEGYIRRVDPEEFRVQKRGGRGVSV
HHHCCCCCCHHHHHHHCCCCCHHHCCCCEEEEEECCCCCEECCCHHHHHHHHCCCCEEEE
NSSNEDPIVIATMGKMRDVLFFTNSGKVFRTKAYNIRQYSRTARGLPIVNFLNGLTAGDK
CCCCCCCEEEEECCCCCEEEEEECCCCEEEEECCCHHHHHHHHCCCCHHHHHHCCCCCCC
ITAILPLRNVKEKMNYLTFITEKGMIKKTDISLFDNINKNGKIAINLKEDDQLVTVFATT
EEEEECCHHHHHHHHHHEEEECCCCEEECCHHHHHCCCCCCEEEEEECCCCCEEEEEEEC
GEDTIFVANKSGKVIRIQENIVRPLSRTASGVKAIKLDENDVVVGAVTSFGIENITTISS
CCCEEEEECCCCCEEEEHHHHHHHHHHHCCCCEEEEECCCCEEEEEHHHCCCCCEEEECC
KGSFKKTNIDEYRISGRNGKGIKVMNLNEKTGDFKSIIAARETDLVCIISTDGNLIKTKA
CCCCCCCCCCCEEEECCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCEEEECC
SDIPVLGRAAAGVRGIRLSEGNKIQAVMLEYRKHGEENQEFEED
CCCCEECHHHCCCCEEEECCCCEEHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SEKNLEHNHGIIKGIDIAAEVRKDFLEYSMSVIVSRALPDLKDGLKPVHRRIIYAMNDL
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GITADKPHKKSARIVGEVIGKYHPHGDTAVYDSMVRMAQDFSYRYPLVDGHGNFGSIDGD
CCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
GAAAMRYTEARLAKVSNFIIKDIDMNTVPFVDNYDASEREPAYLTGYFPNLLANGAMGIA
CCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHCCCCCEEE
VGMATSIPPHNLREVISAIHAFIDNKDITIDEILDNHIMGPDFPTGALMTNGIRMREGYK
EEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCHHHCCCEEEECCCC
TGRGAVTIRAKVALEENDRHARFIITEIPYQTNKAKLIERIAELVKTKQLEGISDIRDES
CCCCEEEEEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCC
NYEGIRIVIELRRDSNPDVVLSKLYKFTNLQTTYSLNLLSLHNNIPVLLDLKSIIKHYVD
CCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCEEEEHHHHHHHHHC
FQINVIIKRSIFEKDKIEKRFHILEALDTALDNIDAIVNILRNSPESNEAKMKLTEAFGF
EEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHCCC
DEEQNKAILDMRLQRLVGLERGKIQQEMAQIKERIDYLTLLISDETEQNNVLKNQLSEIA
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHH
EKFGDNRRTETIDEGLMDIEDEELIPDVKTMILLSDEGYIRRVDPEEFRVQKRGGRGVSV
HHHCCCCCCHHHHHHHCCCCCHHHCCCCEEEEEECCCCCEECCCHHHHHHHHCCCCEEEE
NSSNEDPIVIATMGKMRDVLFFTNSGKVFRTKAYNIRQYSRTARGLPIVNFLNGLTAGDK
CCCCCCCEEEEECCCCCEEEEEECCCCEEEEECCCHHHHHHHHCCCCHHHHHHCCCCCCC
ITAILPLRNVKEKMNYLTFITEKGMIKKTDISLFDNINKNGKIAINLKEDDQLVTVFATT
EEEEECCHHHHHHHHHHEEEECCCCEEECCHHHHHCCCCCCEEEEEECCCCCEEEEEEEC
GEDTIFVANKSGKVIRIQENIVRPLSRTASGVKAIKLDENDVVVGAVTSFGIENITTISS
CCCEEEEECCCCCEEEEHHHHHHHHHHHCCCCEEEEECCCCEEEEEHHHCCCCCEEEECC
KGSFKKTNIDEYRISGRNGKGIKVMNLNEKTGDFKSIIAARETDLVCIISTDGNLIKTKA
CCCCCCCCCCCEEEECCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCEEEECC
SDIPVLGRAAAGVRGIRLSEGNKIQAVMLEYRKHGEENQEFEED
CCCCEECHHHCCCCEEEECCCCEEHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]