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The map label for this gene is gyrB [H]

Identifier: 50364821

GI number: 50364821

Start: 5302

End: 7209

Strand: Direct

Name: gyrB [H]

Synonym: Mfl006

Alternate gene names: 50364821

Gene position: 5302-7209 (Clockwise)

Preceding gene: 50364820

Following gene: 50364822

Centisome position: 0.67

GC content: 33.07

Gene sequence:

>1908_bases
ATGGCAGAACAAAATTATGGTGCTGAATCGATTAAAGTTCTTAAGGGTTTAGAAGCTGTTAGAAAACGTCCAGGTATGTA
TATTGGATCAACTTCTAAAGCAGGTCTACACCACTTAGTTTGAGAAATCATGGATAACTCAGTCGATGAAGCAATGGCAG
GGTTTGCAAACAAAATTATTTTAACTATTACTAAAGAAGGGGAAATCATTGTTCAAGATAATGGACGTGGTATTCCTGTT
GGTATTCAAGCTGACTCAGGGAAATCAGCTTTAGAACTAGTTTTTACTCAGTTACACGCTGGGGGAAAATTTGACTCAGA
TTCATATAAAATTTCAGGAGGATTACATGGGGTTGGTGCCTCAGTTGTTAATGCCTTATCATTATATGTTGATGTTCAAG
TTAAACGTGAAGGTAAAATTCATCATCAAGTATTTAGTGGCGGGGGAACTCAACAAACTGAAATTGAAATTATTGGTGAA
ACAAATGAAACAGGAACAATTGTTAGATTTAAACCAGATCCAGAAATCTTTTTAGAAGGAACTGAATTTGATTATGAAAC
AATTAGAAATAAAGTTAAACAACTTTCATATTTAAATAAAGGTTTAGTAATTGAATTAAACGATTTAAGAATTGACAAAC
ATGTAGAGTACCACTTCCCAAATGGTATCTTGGATTATGTAAAAGAAAAAAATGAAACAAAAGTAAAAATTAACCCAAGC
ATTTTCTATGTTGATGATAAACATGATGATATTGAAGTTGAAGTTGCATTACAATACAACGCAGAATATCAAGAAAACTT
AATCACATTTGTTAACAATATTAATACTCATGAAGGTGGAACTCATGAAGACGGTTTAAGACAATCTTTAGTTAGAGTTA
TTAATCGTTATGCTGAAAAAGTTGCAACAGGTAATAAACCAGCTGCTAAATACTCATGAGATGATATTAAAGAAGGAATG
GTATGTATAGTTTCAATTAGACATACTGATCCACAATATGAAGGGCAAACTAAAACAAAATTAGCTAACCCTGATGCTAA
AAAAGCTGTTGATGCTGTAGTTGGTGATGCATTTGAAGAATTCTTATTAAAATCACCAGAAGATGCAAAAGCAATTATTG
ATAAAAATGCAAATGCTCAAAAAGCTAGAATAGCTGCACAAAGAGCTAGAGAAGAAACTAGAAGAAAATCAGCTCTTGAT
ACATTCTCACTTCCTGGTAAATTAGCGGATTGTGAAAGTAAAGATCCAGAAATCGCTGAACTTTACTTAGTCGAAGGGGA
TTCAGCCGGAGGTTCAGCAAAAACTGGACGTAACCGTAGATTCCAAGCTATCTTACCTTTAAGAGGGAAAGTATTGAACG
TTGAGCGTGTTGCTGAAGTTAGAGCTTTTGCAAATAATGAGATTAAATCAATTGTTACAGCAATTGGTACAGGTATTAAA
GAAGATATTGATTTATCAAAATTAAGATATGGAAAAATTGTTATTATGACTGATGCCGATGTCGATGGTGCTCATATTCG
TACTTTATTATTAACATTCTTTTACAGATACATGAAACAATTAGTTGTTGATGGTCACGTGTACATAGCACAACCACCGC
TTTATAAAATTGAAGCAGGTAAAAAAGTTGCTTATGCATATAGTGATGTTGAACTTGAAGAATTAAAAGCAGGTGACTTT
AAAGATTCAAGATTTACAATTCAACGTTACAAAGGACTTGGAGAAATGGATCCAATCCAATTATGAGAAACAACAATGGA
CCCTGAAAAACGTACAATGTTACAAATTAAATTAGAAGATGCTGCAATCGCTAATGAAGTTTTCTCAGACTTAATGGGAG
AAGACCCAGAACTAAGAAGAAATTATATTCAAGAAAACGCAGAGTTTGTTGAAAACATTGACTTCTAG

Upstream 100 bases:

>100_bases
ATGGGTTTTAACTCCATTGCCTGTTTTTATAGTAAAAATGGCTTAATTAATATATAATATATATTAAGGAAACTTAGAAA
GAAACGGGTGAACAAATTAT

Downstream 100 bases:

>100_bases
ACAAAGAATAGAAAAGGTGAATTAAAATGAGTGAAAAAAACTTAGAACATAACCACGGTATTATCAAGGGTATTGATATT
GCTGCTGAGGTTAGAAAAGA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MAEQNYGAESIKVLKGLEAVRKRPGMYIGSTSKAGLHHLVWEIMDNSVDEAMAGFANKIILTITKEGEIIVQDNGRGIPV
GIQADSGKSALELVFTQLHAGGKFDSDSYKISGGLHGVGASVVNALSLYVDVQVKREGKIHHQVFSGGGTQQTEIEIIGE
TNETGTIVRFKPDPEIFLEGTEFDYETIRNKVKQLSYLNKGLVIELNDLRIDKHVEYHFPNGILDYVKEKNETKVKINPS
IFYVDDKHDDIEVEVALQYNAEYQENLITFVNNINTHEGGTHEDGLRQSLVRVINRYAEKVATGNKPAAKYSWDDIKEGM
VCIVSIRHTDPQYEGQTKTKLANPDAKKAVDAVVGDAFEEFLLKSPEDAKAIIDKNANAQKARIAAQRAREETRRKSALD
TFSLPGKLADCESKDPEIAELYLVEGDSAGGSAKTGRNRRFQAILPLRGKVLNVERVAEVRAFANNEIKSIVTAIGTGIK
EDIDLSKLRYGKIVIMTDADVDGAHIRTLLLTFFYRYMKQLVVDGHVYIAQPPLYKIEAGKKVAYAYSDVELEELKAGDF
KDSRFTIQRYKGLGEMDPIQLWETTMDPEKRTMLQIKLEDAAIANEVFSDLMGEDPELRRNYIQENAEFVENIDF

Sequences:

>Translated_635_residues
MAEQNYGAESIKVLKGLEAVRKRPGMYIGSTSKAGLHHLV*EIMDNSVDEAMAGFANKIILTITKEGEIIVQDNGRGIPV
GIQADSGKSALELVFTQLHAGGKFDSDSYKISGGLHGVGASVVNALSLYVDVQVKREGKIHHQVFSGGGTQQTEIEIIGE
TNETGTIVRFKPDPEIFLEGTEFDYETIRNKVKQLSYLNKGLVIELNDLRIDKHVEYHFPNGILDYVKEKNETKVKINPS
IFYVDDKHDDIEVEVALQYNAEYQENLITFVNNINTHEGGTHEDGLRQSLVRVINRYAEKVATGNKPAAKYS*DDIKEGM
VCIVSIRHTDPQYEGQTKTKLANPDAKKAVDAVVGDAFEEFLLKSPEDAKAIIDKNANAQKARIAAQRAREETRRKSALD
TFSLPGKLADCESKDPEIAELYLVEGDSAGGSAKTGRNRRFQAILPLRGKVLNVERVAEVRAFANNEIKSIVTAIGTGIK
EDIDLSKLRYGKIVIMTDADVDGAHIRTLLLTFFYRYMKQLVVDGHVYIAQPPLYKIEAGKKVAYAYSDVELEELKAGDF
KDSRFTIQRYKGLGEMDPIQL*ETTMDPEKRTMLQIKLEDAAIANEVFSDLMGEDPELRRNYIQENAEFVENIDF
>Mature_634_residues
AEQNYGAESIKVLKGLEAVRKRPGMYIGSTSKAGLHHLV*EIMDNSVDEAMAGFANKIILTITKEGEIIVQDNGRGIPVG
IQADSGKSALELVFTQLHAGGKFDSDSYKISGGLHGVGASVVNALSLYVDVQVKREGKIHHQVFSGGGTQQTEIEIIGET
NETGTIVRFKPDPEIFLEGTEFDYETIRNKVKQLSYLNKGLVIELNDLRIDKHVEYHFPNGILDYVKEKNETKVKINPSI
FYVDDKHDDIEVEVALQYNAEYQENLITFVNNINTHEGGTHEDGLRQSLVRVINRYAEKVATGNKPAAKYS*DDIKEGMV
CIVSIRHTDPQYEGQTKTKLANPDAKKAVDAVVGDAFEEFLLKSPEDAKAIIDKNANAQKARIAAQRAREETRRKSALDT
FSLPGKLADCESKDPEIAELYLVEGDSAGGSAKTGRNRRFQAILPLRGKVLNVERVAEVRAFANNEIKSIVTAIGTGIKE
DIDLSKLRYGKIVIMTDADVDGAHIRTLLLTFFYRYMKQLVVDGHVYIAQPPLYKIEAGKKVAYAYSDVELEELKAGDFK
DSRFTIQRYKGLGEMDPIQL*ETTMDPEKRTMLQIKLEDAAIANEVFSDLMGEDPELRRNYIQENAEFVENIDF

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=640, Percent_Identity=26.40625, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI19913408, Length=608, Percent_Identity=26.1513157894737, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI48994957, Length=556, Percent_Identity=51.6187050359712, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI1789408, Length=639, Percent_Identity=39.4366197183099, Blast_Score=427, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI212645845, Length=537, Percent_Identity=28.1191806331471, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17535065, Length=605, Percent_Identity=26.7768595041322, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI212645657, Length=176, Percent_Identity=30.6818181818182, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=620, Percent_Identity=25.6451612903226, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17136538, Length=598, Percent_Identity=24.4147157190635, Blast_Score=126, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 70379; Mature: 70247

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQNYGAESIKVLKGLEAVRKRPGMYIGSTSKAGLHHLVEIMDNSVDEAMAGFANKIIL
CCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHCCCEEEE
TITKEGEIIVQDNGRGIPVGIQADSGKSALELVFTQLHAGGKFDSDSYKISGGLHGVGAS
EEECCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHH
VVNALSLYVDVQVKREGKIHHQVFSGGGTQQTEIEIIGETNETGTIVRFKPDPEIFLEGT
HHHHHEEEEEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEECC
EFDYETIRNKVKQLSYLNKGLVIELNDLRIDKHVEYHFPNGILDYVKEKNETKVKINPSI
CCCHHHHHHHHHHHHHHCCCCEEEEECEEEECEEEEECCCHHHHHHHCCCCCEEEECCEE
FYVDDKHDDIEVEVALQYNAEYQENLITFVNNINTHEGGTHEDGLRQSLVRVINRYAEKV
EEEECCCCCEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ATGNKPAAKYSDDIKEGMVCIVSIRHTDPQYEGQTKTKLANPDAKKAVDAVVGDAFEEFL
HCCCCCCHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LKSPEDAKAIIDKNANAQKARIAAQRAREETRRKSALDTFSLPGKLADCESKDPEIAELY
HCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
LVEGDSAGGSAKTGRNRRFQAILPLRGKVLNVERVAEVRAFANNEIKSIVTAIGTGIKED
EEECCCCCCCCCCCCCCEEEEEECCCCCEECHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
IDLSKLRYGKIVIMTDADVDGAHIRTLLLTFFYRYMKQLVVDGHVYIAQPPLYKIEAGKK
CCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCE
VAYAYSDVELEELKAGDFKDSRFTIQRYKGLGEMDPIQLETTMDPEKRTMLQIKLEDAAI
EEEEECCCCHHHHCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECHHHH
ANEVFSDLMGEDPELRRNYIQENAEFVENIDF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEQNYGAESIKVLKGLEAVRKRPGMYIGSTSKAGLHHLVEIMDNSVDEAMAGFANKIIL
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHCCCEEEE
TITKEGEIIVQDNGRGIPVGIQADSGKSALELVFTQLHAGGKFDSDSYKISGGLHGVGAS
EEECCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHH
VVNALSLYVDVQVKREGKIHHQVFSGGGTQQTEIEIIGETNETGTIVRFKPDPEIFLEGT
HHHHHEEEEEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEECC
EFDYETIRNKVKQLSYLNKGLVIELNDLRIDKHVEYHFPNGILDYVKEKNETKVKINPSI
CCCHHHHHHHHHHHHHHCCCCEEEEECEEEECEEEEECCCHHHHHHHCCCCCEEEECCEE
FYVDDKHDDIEVEVALQYNAEYQENLITFVNNINTHEGGTHEDGLRQSLVRVINRYAEKV
EEEECCCCCEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ATGNKPAAKYSDDIKEGMVCIVSIRHTDPQYEGQTKTKLANPDAKKAVDAVVGDAFEEFL
HCCCCCCHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LKSPEDAKAIIDKNANAQKARIAAQRAREETRRKSALDTFSLPGKLADCESKDPEIAELY
HCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
LVEGDSAGGSAKTGRNRRFQAILPLRGKVLNVERVAEVRAFANNEIKSIVTAIGTGIKED
EEECCCCCCCCCCCCCCEEEEEECCCCCEECHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
IDLSKLRYGKIVIMTDADVDGAHIRTLLLTFFYRYMKQLVVDGHVYIAQPPLYKIEAGKK
CCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCE
VAYAYSDVELEELKAGDFKDSRFTIQRYKGLGEMDPIQLETTMDPEKRTMLQIKLEDAAI
EEEEECCCCHHHHCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECHHHH
ANEVFSDLMGEDPELRRNYIQENAEFVENIDF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7764984 [H]