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The map label for this gene is ilvD2

Identifier: 50086579

GI number: 50086579

Start: 3547778

End: 3549463

Strand: Reverse

Name: ilvD2

Synonym: ACIAD3636

Alternate gene names: 50086579

Gene position: 3549463-3547778 (Counterclockwise)

Preceding gene: 50086582

Following gene: 50086578

Centisome position: 98.63

GC content: 46.8

Gene sequence:

>1686_bases
ATGAGTAAAGACAATATACGCCAGTATTCGGCACCAGTTTATGACGGTATTGAAAATGCCCCTGCACGTTCGATGATGCG
TGCTGTGGGCTTTCAAGATAAAGATTTTAATCGTCCATTTATTGGCATTGCTTCAACATGGGCCAATGTCACGCCGTGCA
ACATGCACATTGATGGACTGGCACGAGAAGCTGAACATGGGGTGAATCAGGCCAGCGGTAAGGGAATTATTTTTAACACT
ATTACCATTTCTGACGGTATTTCAAACGGTACCGAAGGCATGAAGTATTCTTTGGTTTCTCGTGAAATTATTGCAGACTC
TATTGAGGCGGTCATTGGTTGTCAGGGTTACGATGGTGTAATTGCCATTGGTGGCTGCGACAAAAACATGCCTGGCTGTA
TTATGGGGCTGGCACGTTTGAACCGTCCAGGCTTGTTTATTTATGGCGGCACCATTCAACCAGGTGAAGGACATACCGAC
ATGATTTCGGTGTTTGAAGCGGTGGGACAGTACGCCAAAGGAGAAATCAATGCCATTCAGGTCAAGCAGATTGAAGAAGT
CGCTTTACCAGGACCAGGTTCTTGCGGGGGAATGTATACCGCCAATACCATGGCTTCTGCTATTGAAGCACTGGGAATGA
GTTTACCAGGTTCTTCTGCACAGGATGCCATTTCTGAAGAAAAGCGTCAGGACTGTCATCGTGCGGGCGAGGCGGTGATG
AACCTGCTTCGGCTGGATATTCGTCCACGCGATATTATGACCAAAGCGGCATTTGAGAATGCCATTAAGGTCGTCATTGC
TTTGGGCGGTTCAACCAATGCCGTATTGCATTTGATTGCAATGGCACATACGGCTGAGGTAAAGCTTGATCTGGATGATT
TTGTACGAATTGGTCAGGAAACACCTGTGGTGGCAGATTTACGACCATCGGGTAAGTATCTCATGTCCGAACTGATCAAG
ATTGGTGGGATTCAGCCGTTGATGAAGCGAATGCTGGAACGCGGCATGCTGGATGGTAGTTGTCTGACTGTAACTGGTAA
GACCCTTGCAGAGAATTTGGCCGAGGTTGAGGATTATCCTGCCGATCAACAAATTATCTTGCCATTTGAGCAACCTGTTA
AGAAAGACTCCCATCTAGTGATTCTTAAGGGTAATTTGTCCCCGACAGGTGCCGTTGCCAAAATTACGGGCAAAGAAGGT
TTATATTTTGAAGGGCCAGCCCGCGTTTTTGAAGGCGAAGAAGGCGCCATGCGTGGCATTCTAGACGGAGAAGTACAAGA
GGGCGAAGTTGTCGTCATTCGTGGTGAAGGACCCAAAGGTGGACCAGGCATGCGCGAAATGCTGAAACCAACGTCTGCAA
TTATTGGTAAAGGACTGGGACAATCGGTGGCGCTGATTACCGATGGGCGGTTTTCGGGCGGCAGTCATGGCTTTGTCATT
GGCCATGTGACCCCAGAAGCTTACGAAGGTGGGCCTATTGGTTTGGTTGAAAATGGTGATAAAATTTCAATCAATGCTGA
AACGCGTGAAATCACCTTACATATTTCTAACGAAGAAATGAGCTTACGTCGTGCAAAATGGCAGAAACCAGAACCAAAAT
ATAAATCTGGTGCTTTGGCAAAATTTGCAAAACTTGCAGCAGGTGCGGACAAAGGCGCAGTTACTGATTTAAATCTTGAT
GTGTAA

Upstream 100 bases:

>100_bases
TTTCGCTGTTAGTATTGATTTACGACAGATGGAAATAATAAAGAGTCCCATGATCACCCGCGTAGCGATGGGATCTTGCC
TCAGCCACGGAGAATGACGC

Downstream 100 bases:

>100_bases
TTGATCATTTTGTCCTGAATTGTTATAAACCCTTGTAAGATTACAAGGGTTTATTATTCGAGGCGCTCTTCATGTCACTG
TTAAGACGTTGGTTTGATCC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 2

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MSKDNIRQYSAPVYDGIENAPARSMMRAVGFQDKDFNRPFIGIASTWANVTPCNMHIDGLAREAEHGVNQASGKGIIFNT
ITISDGISNGTEGMKYSLVSREIIADSIEAVIGCQGYDGVIAIGGCDKNMPGCIMGLARLNRPGLFIYGGTIQPGEGHTD
MISVFEAVGQYAKGEINAIQVKQIEEVALPGPGSCGGMYTANTMASAIEALGMSLPGSSAQDAISEEKRQDCHRAGEAVM
NLLRLDIRPRDIMTKAAFENAIKVVIALGGSTNAVLHLIAMAHTAEVKLDLDDFVRIGQETPVVADLRPSGKYLMSELIK
IGGIQPLMKRMLERGMLDGSCLTVTGKTLAENLAEVEDYPADQQIILPFEQPVKKDSHLVILKGNLSPTGAVAKITGKEG
LYFEGPARVFEGEEGAMRGILDGEVQEGEVVVIRGEGPKGGPGMREMLKPTSAIIGKGLGQSVALITDGRFSGGSHGFVI
GHVTPEAYEGGPIGLVENGDKISINAETREITLHISNEEMSLRRAKWQKPEPKYKSGALAKFAKLAAGADKGAVTDLNLD
V

Sequences:

>Translated_561_residues
MSKDNIRQYSAPVYDGIENAPARSMMRAVGFQDKDFNRPFIGIASTWANVTPCNMHIDGLAREAEHGVNQASGKGIIFNT
ITISDGISNGTEGMKYSLVSREIIADSIEAVIGCQGYDGVIAIGGCDKNMPGCIMGLARLNRPGLFIYGGTIQPGEGHTD
MISVFEAVGQYAKGEINAIQVKQIEEVALPGPGSCGGMYTANTMASAIEALGMSLPGSSAQDAISEEKRQDCHRAGEAVM
NLLRLDIRPRDIMTKAAFENAIKVVIALGGSTNAVLHLIAMAHTAEVKLDLDDFVRIGQETPVVADLRPSGKYLMSELIK
IGGIQPLMKRMLERGMLDGSCLTVTGKTLAENLAEVEDYPADQQIILPFEQPVKKDSHLVILKGNLSPTGAVAKITGKEG
LYFEGPARVFEGEEGAMRGILDGEVQEGEVVVIRGEGPKGGPGMREMLKPTSAIIGKGLGQSVALITDGRFSGGSHGFVI
GHVTPEAYEGGPIGLVENGDKISINAETREITLHISNEEMSLRRAKWQKPEPKYKSGALAKFAKLAAGADKGAVTDLNLD
V
>Mature_560_residues
SKDNIRQYSAPVYDGIENAPARSMMRAVGFQDKDFNRPFIGIASTWANVTPCNMHIDGLAREAEHGVNQASGKGIIFNTI
TISDGISNGTEGMKYSLVSREIIADSIEAVIGCQGYDGVIAIGGCDKNMPGCIMGLARLNRPGLFIYGGTIQPGEGHTDM
ISVFEAVGQYAKGEINAIQVKQIEEVALPGPGSCGGMYTANTMASAIEALGMSLPGSSAQDAISEEKRQDCHRAGEAVMN
LLRLDIRPRDIMTKAAFENAIKVVIALGGSTNAVLHLIAMAHTAEVKLDLDDFVRIGQETPVVADLRPSGKYLMSELIKI
GGIQPLMKRMLERGMLDGSCLTVTGKTLAENLAEVEDYPADQQIILPFEQPVKKDSHLVILKGNLSPTGAVAKITGKEGL
YFEGPARVFEGEEGAMRGILDGEVQEGEVVVIRGEGPKGGPGMREMLKPTSAIIGKGLGQSVALITDGRFSGGSHGFVIG
HVTPEAYEGGPIGLVENGDKISINAETREITLHISNEEMSLRRAKWQKPEPKYKSGALAKFAKLAAGADKGAVTDLNLDV

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=605, Percent_Identity=37.6859504132231, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1788157, Length=520, Percent_Identity=29.8076923076923, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI2367371, Length=459, Percent_Identity=32.6797385620915, Blast_Score=196, Evalue=5e-51,
Organism=Escherichia coli, GI1786464, Length=466, Percent_Identity=33.0472103004292, Blast_Score=188, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6322476, Length=562, Percent_Identity=51.067615658363, Blast_Score=584, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD2_ACIAD (Q6F6Q0)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_048089.1
- STRING:   Q6F6Q0
- GeneID:   2878980
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3636
- NMPDR:   fig|62977.3.peg.3282
- eggNOG:   COG0129
- HOGENOM:   HBG671001
- OMA:   NMPGAMI
- PhylomeDB:   Q6F6Q0
- ProtClustDB:   PRK00911
- BioCyc:   ASP62977:ACIAD3636-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59681; Mature: 59550

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDNIRQYSAPVYDGIENAPARSMMRAVGFQDKDFNRPFIGIASTWANVTPCNMHIDGL
CCCCCCHHHCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEHHHH
AREAEHGVNQASGKGIIFNTITISDGISNGTEGMKYSLVSREIIADSIEAVIGCQGYDGV
HHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHEECCCCCCE
IAIGGCDKNMPGCIMGLARLNRPGLFIYGGTIQPGEGHTDMISVFEAVGQYAKGEINAIQ
EEECCCCCCCCHHHHHHHHCCCCCEEEECCEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
VKQIEEVALPGPGSCGGMYTANTMASAIEALGMSLPGSSAQDAISEEKRQDCHRAGEAVM
EEHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NLLRLDIRPRDIMTKAAFENAIKVVIALGGSTNAVLHLIAMAHTAEVKLDLDDFVRIGQE
HHHHHCCCCHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHCEEEECHHHHHHCCCC
TPVVADLRPSGKYLMSELIKIGGIQPLMKRMLERGMLDGSCLTVTGKTLAENLAEVEDYP
CCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCC
ADQQIILPFEQPVKKDSHLVILKGNLSPTGAVAKITGKEGLYFEGPARVFEGEEGAMRGI
CCCEEEECCHHCCCCCCEEEEEECCCCCCCCEEEEECCCCEEECCCHHEECCCCCCCCCC
LDGEVQEGEVVVIRGEGPKGGPGMREMLKPTSAIIGKGLGQSVALITDGRFSGGSHGFVI
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEE
GHVTPEAYEGGPIGLVENGDKISINAETREITLHISNEEMSLRRAKWQKPEPKYKSGALA
EECCCCCCCCCCEEEEECCCEEEEEECEEEEEEEECCCHHHHHHHHCCCCCCCCCCCHHH
KFAKLAAGADKGAVTDLNLDV
HHHHHHCCCCCCCEEEEEECC
>Mature Secondary Structure 
SKDNIRQYSAPVYDGIENAPARSMMRAVGFQDKDFNRPFIGIASTWANVTPCNMHIDGL
CCCCCHHHCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEHHHH
AREAEHGVNQASGKGIIFNTITISDGISNGTEGMKYSLVSREIIADSIEAVIGCQGYDGV
HHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHEECCCCCCE
IAIGGCDKNMPGCIMGLARLNRPGLFIYGGTIQPGEGHTDMISVFEAVGQYAKGEINAIQ
EEECCCCCCCCHHHHHHHHCCCCCEEEECCEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
VKQIEEVALPGPGSCGGMYTANTMASAIEALGMSLPGSSAQDAISEEKRQDCHRAGEAVM
EEHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NLLRLDIRPRDIMTKAAFENAIKVVIALGGSTNAVLHLIAMAHTAEVKLDLDDFVRIGQE
HHHHHCCCCHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHCEEEECHHHHHHCCCC
TPVVADLRPSGKYLMSELIKIGGIQPLMKRMLERGMLDGSCLTVTGKTLAENLAEVEDYP
CCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCC
ADQQIILPFEQPVKKDSHLVILKGNLSPTGAVAKITGKEGLYFEGPARVFEGEEGAMRGI
CCCEEEECCHHCCCCCCEEEEEECCCCCCCCEEEEECCCCEEECCCHHEECCCCCCCCCC
LDGEVQEGEVVVIRGEGPKGGPGMREMLKPTSAIIGKGLGQSVALITDGRFSGGSHGFVI
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEE
GHVTPEAYEGGPIGLVENGDKISINAETREITLHISNEEMSLRRAKWQKPEPKYKSGALA
EECCCCCCCCCCEEEEECCCEEEEEECEEEEEEEECCCHHHHHHHHCCCCCCCCCCCHHH
KFAKLAAGADKGAVTDLNLDV
HHHHHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA