Click here to switch to the map view.

The map label for this gene is ybdL [H]

Identifier: 50086507

GI number: 50086507

Start: 3477927

End: 3479081

Strand: Direct

Name: ybdL [H]

Synonym: ACIAD3553

Alternate gene names: 50086507

Gene position: 3477927-3479081 (Clockwise)

Preceding gene: 50086506

Following gene: 50086508

Centisome position: 96.65

GC content: 45.54

Gene sequence:

>1155_bases
ATGCAGCCGCTTTCATCTAAACTTCCCAATCAGGGCGTTACCATTTTTAGTACCATGACAGCATTGGCACAGCGTCTAAA
TGCAATTAATCTATCTCAGGGTTTTCCAGATTTTGCGACACCTGATGCCTTGATCGAAGCCCTTTGTGATGCGACTCGTG
CAGGTTTTAATCAGTACGTATCTGGAGATGGCTGGTTACCATTACGTGAGCAGGTTGTGCAACAATTTGAACAACGTGAC
CAAGTGCGGCTTGATCCAGTTCATGAAATTACCATTACCCCAGGCGCGACCATTGCGATCTTTGCTGCCATTCAGGCTGT
TATTCATACGGGTGATGAAGTTGTTATTTTCGATCCAAGTTATGACAGCTATGCGCCGGCTGTACGTGTGGCGGGTGGAA
TACCTGTGCATCTTGCACTTACGGCTCCTGATTTTCGGGTAGACTGGCAACAGGTTCAAGACAATATCAACGCCCGTACT
CGCATGATTATTGTGAATACACCACATAATCCTACTGGTACTTTATGGACACTGGATGATTGGCATCACCTCATTGAACT
GGCAGAAAAGCATGACCTGATTGTTTTGTCCGATGAGGTCTATGAACATCTGGTTTTTGATGGACATCATCATTATAGTG
CGCTGCATTTTTCTACGTTGCGCGAACGCAGCTTTGTTGTCGGTTCATTTGGTAAGACGTTTCATGTCACTGGATGGAAA
ACGGGTTACTGTGTTGCAAGCCCGATGCTCATGAAACTCTTCCGACAGATTTATCAATACGCCAATTTCTGTGGTAATAC
GCCTGCCCAAGTTGCGCTTAGCCAGTATATGCAGCAGCATCCAGAGCATATTCAGGAGCTTGGAAGTTTCTATCAGGCCA
AACGCGATCTGTTTAATCAAGGGCTAACTCAATCGAGATTTAAGTTTCAACCGACCCACGGCACTTACTTCCAAACGGTA
GACTACACTGCGATACGCCCAGATCTGAACGATCTTGAAATGTGCTATTTTTTAGCCGAACAGTATGGGGTGGTTGCGAT
TCCGTTGTCGGTGTTTTATCAACAGCCCGATCCTGAGTTACGTTTGATTCGATTCTGTTTTGCTAAAACAGAGCAGACCC
TAAAAGATGCCACCCAGCGTCTGAGTCAATGTTAA

Upstream 100 bases:

>100_bases
GTGGTGGTAGGGGCATTTATTGGATTAATCCTAGGGATTATCCGTGCACTTTTCGATTATATGCTATAACCCTAACATGA
TTTTTATTTGAACTGATTCT

Downstream 100 bases:

>100_bases
TCCCGACACACCCATCTATAGCAAAAATCACACTTAATCTAGAGTCTGACTATGCTATGTTCTGAAACATCGCCTTTCAG
ATTACTTCGTGCGCCAAGGA

Product: aminotransferase

Products: oxaloacetate; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MQPLSSKLPNQGVTIFSTMTALAQRLNAINLSQGFPDFATPDALIEALCDATRAGFNQYVSGDGWLPLREQVVQQFEQRD
QVRLDPVHEITITPGATIAIFAAIQAVIHTGDEVVIFDPSYDSYAPAVRVAGGIPVHLALTAPDFRVDWQQVQDNINART
RMIIVNTPHNPTGTLWTLDDWHHLIELAEKHDLIVLSDEVYEHLVFDGHHHYSALHFSTLRERSFVVGSFGKTFHVTGWK
TGYCVASPMLMKLFRQIYQYANFCGNTPAQVALSQYMQQHPEHIQELGSFYQAKRDLFNQGLTQSRFKFQPTHGTYFQTV
DYTAIRPDLNDLEMCYFLAEQYGVVAIPLSVFYQQPDPELRLIRFCFAKTEQTLKDATQRLSQC

Sequences:

>Translated_384_residues
MQPLSSKLPNQGVTIFSTMTALAQRLNAINLSQGFPDFATPDALIEALCDATRAGFNQYVSGDGWLPLREQVVQQFEQRD
QVRLDPVHEITITPGATIAIFAAIQAVIHTGDEVVIFDPSYDSYAPAVRVAGGIPVHLALTAPDFRVDWQQVQDNINART
RMIIVNTPHNPTGTLWTLDDWHHLIELAEKHDLIVLSDEVYEHLVFDGHHHYSALHFSTLRERSFVVGSFGKTFHVTGWK
TGYCVASPMLMKLFRQIYQYANFCGNTPAQVALSQYMQQHPEHIQELGSFYQAKRDLFNQGLTQSRFKFQPTHGTYFQTV
DYTAIRPDLNDLEMCYFLAEQYGVVAIPLSVFYQQPDPELRLIRFCFAKTEQTLKDATQRLSQC
>Mature_384_residues
MQPLSSKLPNQGVTIFSTMTALAQRLNAINLSQGFPDFATPDALIEALCDATRAGFNQYVSGDGWLPLREQVVQQFEQRD
QVRLDPVHEITITPGATIAIFAAIQAVIHTGDEVVIFDPSYDSYAPAVRVAGGIPVHLALTAPDFRVDWQQVQDNINART
RMIIVNTPHNPTGTLWTLDDWHHLIELAEKHDLIVLSDEVYEHLVFDGHHHYSALHFSTLRERSFVVGSFGKTFHVTGWK
TGYCVASPMLMKLFRQIYQYANFCGNTPAQVALSQYMQQHPEHIQELGSFYQAKRDLFNQGLTQSRFKFQPTHGTYFQTV
DYTAIRPDLNDLEMCYFLAEQYGVVAIPLSVFYQQPDPELRLIRFCFAKTEQTLKDATQRLSQC

Specific function: Shows aminotransferase activity with methionine and histidine as substrates, and to a lesser extent also with phenylalanine [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI95147551, Length=394, Percent_Identity=31.2182741116751, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI169881279, Length=394, Percent_Identity=31.2182741116751, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI56713254, Length=394, Percent_Identity=32.741116751269, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI56713256, Length=394, Percent_Identity=32.741116751269, Blast_Score=208, Evalue=5e-54,
Organism=Homo sapiens, GI169881281, Length=392, Percent_Identity=28.0612244897959, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4507369, Length=377, Percent_Identity=22.8116710875332, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786816, Length=378, Percent_Identity=49.2063492063492, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1788722, Length=377, Percent_Identity=23.0769230769231, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788627, Length=314, Percent_Identity=27.7070063694268, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787909, Length=355, Percent_Identity=22.8169014084507, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994472, Length=402, Percent_Identity=31.3432835820896, Blast_Score=220, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI71994476, Length=402, Percent_Identity=31.3432835820896, Blast_Score=220, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI17567369, Length=400, Percent_Identity=29.5, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17567663, Length=194, Percent_Identity=27.319587628866, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322401, Length=383, Percent_Identity=27.4151436031332, Blast_Score=161, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28573069, Length=410, Percent_Identity=29.5121951219512, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI24646114, Length=410, Percent_Identity=29.5121951219512, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI28573067, Length=410, Percent_Identity=29.5121951219512, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI28573065, Length=410, Percent_Identity=29.5121951219512, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI18859735, Length=259, Percent_Identity=26.6409266409266, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.1

Molecular weight: Translated: 43604; Mature: 43604

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLSSKLPNQGVTIFSTMTALAQRLNAINLSQGFPDFATPDALIEALCDATRAGFNQYV
CCCCCHHCCCCCCHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
SGDGWLPLREQVVQQFEQRDQVRLDPVHEITITPGATIAIFAAIQAVIHTGDEVVIFDPS
CCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCC
YDSYAPAVRVAGGIPVHLALTAPDFRVDWQQVQDNINARTRMIIVNTPHNPTGTLWTLDD
CCCCCCCEEECCCCEEEEEEECCCCCCCHHHHHCCCCCEEEEEEEECCCCCCCCEEEHHH
WHHLIELAEKHDLIVLSDEVYEHLVFDGHHHYSALHFSTLRERSFVVGSFGKTFHVTGWK
HHHHHHHHHCCCEEEECHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCEEEECCCC
TGYCVASPMLMKLFRQIYQYANFCGNTPAQVALSQYMQQHPEHIQELGSFYQAKRDLFNQ
CCHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
GLTQSRFKFQPTHGTYFQTVDYTAIRPDLNDLEMCYFLAEQYGVVAIPLSVFYQQPDPEL
CCCCCCCEECCCCCCEEEEECCEEECCCCCHHHHHHHHHHHCCEEEEEHHHHCCCCCCHH
RLIRFCFAKTEQTLKDATQRLSQC
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQPLSSKLPNQGVTIFSTMTALAQRLNAINLSQGFPDFATPDALIEALCDATRAGFNQYV
CCCCCHHCCCCCCHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
SGDGWLPLREQVVQQFEQRDQVRLDPVHEITITPGATIAIFAAIQAVIHTGDEVVIFDPS
CCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCC
YDSYAPAVRVAGGIPVHLALTAPDFRVDWQQVQDNINARTRMIIVNTPHNPTGTLWTLDD
CCCCCCCEEECCCCEEEEEEECCCCCCCHHHHHCCCCCEEEEEEEECCCCCCCCEEEHHH
WHHLIELAEKHDLIVLSDEVYEHLVFDGHHHYSALHFSTLRERSFVVGSFGKTFHVTGWK
HHHHHHHHHCCCEEEECHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCEEEECCCC
TGYCVASPMLMKLFRQIYQYANFCGNTPAQVALSQYMQQHPEHIQELGSFYQAKRDLFNQ
CCHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
GLTQSRFKFQPTHGTYFQTVDYTAIRPDLNDLEMCYFLAEQYGVVAIPLSVFYQQPDPEL
CCCCCCCEECCCCCCEEEEECCEEECCCCCHHHHHHHHHHHCCEEEEEHHHHCCCCCCHH
RLIRFCFAKTEQTLKDATQRLSQC
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate; 2-oxoglutarate

Specific reaction: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]