Click here to switch to the map view.

The map label for this gene is pyrE [H]

Identifier: 50086481

GI number: 50086481

Start: 3453068

End: 3453781

Strand: Reverse

Name: pyrE [H]

Synonym: ACIAD3525

Alternate gene names: 50086481

Gene position: 3453781-3453068 (Counterclockwise)

Preceding gene: 50086484

Following gene: 50086480

Centisome position: 95.98

GC content: 45.94

Gene sequence:

>714_bases
TTGGATAGCTATCCTTTGGTATCATGCGCAAGTTTGACCTCAATTGATGAATCTGGAGCACCTATGACAACGCCATCGTC
TTTTAACCCGCAAGCCTTTATCGAACTCGCGTTATCACGCGGTGTGCTTAAATTTGGTGAGTTTACCTTAAAATCAGGGC
GTGTTAGTCCGTATTTTTTTAACGCAGGTTTGTTAAACGATGGTGAGGCATTGTCACTTTTGGCGCAAGGTTATGCCGAT
CAATTGATGCAATGTCAGCATGTCGACGTCATTTTTGGGCCTGCATACAAAGGTATTCCATTTGTGGCTGCAACGGCAGT
GGCATTATCACAACTGCATGCTAAGAGTGTGCCTTGGGGCTTTAACCGTAAAGAAGCCAAAGATCATGGTGAAGGCGGTG
TGTTGGTCGGTGCATCGGTTGAGGGCAAAAAAGTCTGGATCATCGATGATGTCATCACTGCGGGCACAGCCATTCGTGAA
GTAGTGACTATCTTGAAAAATGCGGGCGCAACCATTGCGGGTGTGCTGGTGGCGCTGGATCGTCAGGAACGTGGTCAGGG
CCAATTATCCGCTATTCAGGAAGTTCAACAAGAACTTGAGATTCCTGTACATGCATTGATTACCATGAAAGACTTAATGA
ATTATCTTGATGCGAAAGGCGAAACACAGGCGTTGGCTAAAATGGAAGATTATCGTCTGAAATACGGCATCTGA

Upstream 100 bases:

>100_bases
TCTGCTTCGGATTTTTCGAGCCATTCGAGCAGCCCTTTGGTCACAGATGAACGTAAGCCATTTACGTTGATGGAAACCAC
ACGTAAAATTTTTACATCAC

Downstream 100 bases:

>100_bases
TTTCAAGTGAAAAAGGAAGCACAAGCTTCCTTTTTCACTAAGGTGCGATTATCTAAAATTATAAAAATAAATCATCCCAT
TTGATCTGGTTTGTCAAAAA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MDSYPLVSCASLTSIDESGAPMTTPSSFNPQAFIELALSRGVLKFGEFTLKSGRVSPYFFNAGLLNDGEALSLLAQGYAD
QLMQCQHVDVIFGPAYKGIPFVAATAVALSQLHAKSVPWGFNRKEAKDHGEGGVLVGASVEGKKVWIIDDVITAGTAIRE
VVTILKNAGATIAGVLVALDRQERGQGQLSAIQEVQQELEIPVHALITMKDLMNYLDAKGETQALAKMEDYRLKYGI

Sequences:

>Translated_237_residues
MDSYPLVSCASLTSIDESGAPMTTPSSFNPQAFIELALSRGVLKFGEFTLKSGRVSPYFFNAGLLNDGEALSLLAQGYAD
QLMQCQHVDVIFGPAYKGIPFVAATAVALSQLHAKSVPWGFNRKEAKDHGEGGVLVGASVEGKKVWIIDDVITAGTAIRE
VVTILKNAGATIAGVLVALDRQERGQGQLSAIQEVQQELEIPVHALITMKDLMNYLDAKGETQALAKMEDYRLKYGI
>Mature_237_residues
MDSYPLVSCASLTSIDESGAPMTTPSSFNPQAFIELALSRGVLKFGEFTLKSGRVSPYFFNAGLLNDGEALSLLAQGYAD
QLMQCQHVDVIFGPAYKGIPFVAATAVALSQLHAKSVPWGFNRKEAKDHGEGGVLVGASVEGKKVWIIDDVITAGTAIRE
VVTILKNAGATIAGVLVALDRQERGQGQLSAIQEVQQELEIPVHALITMKDLMNYLDAKGETQALAKMEDYRLKYGI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=188, Percent_Identity=28.7234042553192, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790073, Length=209, Percent_Identity=57.4162679425837, Blast_Score=238, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17554964, Length=186, Percent_Identity=27.4193548387097, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508631, Length=199, Percent_Identity=25.1256281407035, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323530, Length=212, Percent_Identity=46.6981132075472, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6323927, Length=215, Percent_Identity=43.7209302325581, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 25462; Mature: 25462

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSYPLVSCASLTSIDESGAPMTTPSSFNPQAFIELALSRGVLKFGEFTLKSGRVSPYFF
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHCCEEECCCCCCCEEE
NAGLLNDGEALSLLAQGYADQLMQCQHVDVIFGPAYKGIPFVAATAVALSQLHAKSVPWG
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
FNRKEAKDHGEGGVLVGASVEGKKVWIIDDVITAGTAIREVVTILKNAGATIAGVLVALD
CCCCHHCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
RQERGQGQLSAIQEVQQELEIPVHALITMKDLMNYLDAKGETQALAKMEDYRLKYGI
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDSYPLVSCASLTSIDESGAPMTTPSSFNPQAFIELALSRGVLKFGEFTLKSGRVSPYFF
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHCCEEECCCCCCCEEE
NAGLLNDGEALSLLAQGYADQLMQCQHVDVIFGPAYKGIPFVAATAVALSQLHAKSVPWG
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
FNRKEAKDHGEGGVLVGASVEGKKVWIIDDVITAGTAIREVVTILKNAGATIAGVLVALD
CCCCHHCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
RQERGQGQLSAIQEVQQELEIPVHALITMKDLMNYLDAKGETQALAKMEDYRLKYGI
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA